pcr mixtures  (Roche)


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    Structured Review

    Roche pcr mixtures
    Pcr Mixtures, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixtures/product/Roche
    Average 92 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    pcr mixtures - by Bioz Stars, 2020-09
    92/100 stars

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    Polymerase Chain Reaction:

    Article Title: Global radiation in a rare biosphere soil diatom
    Article Snippet: .. The PCR mixtures contained per sample 1 µL of the template DNA, 0.4 µM of each primer, 200 µM of each deoxynucleotide triphosphate, 2.5 µL of 10x PCR buffer, and 0.25U of Fast Start High fidelity Taq polymerase (Roche Inc.). .. The final reaction volume was adjusted to 25 µL using HPLC water.

    Article Title: The Native Bacterioplankton Community in the Central Baltic Sea Is Influenced by Freshwater Bacterial Species ▿The Native Bacterioplankton Community in the Central Baltic Sea Is Influenced by Freshwater Bacterial Species ▿ †
    Article Snippet: .. PCR mixtures (50 μl) contained 0.8 mM deoxynucleoside triphosphates, 0.2 μM of primers 27F and 1492R , 100 ng bovine serum albumin, 1× PCR buffer with MgCl2 , and 1 U Taq DNA polymerase (Roche). .. The PCR thermal cycling program was as follows: 95°C for 2 min; 30 cycles of 95°C for 30 s, 50°C for 30 s, and 72°C for 45 s; and 72°C for 7 min. PCR products were cleaved using HaeIII and NdeII (Roche) and analyzed on 2% agarose gels.

    Article Title: Biochar Application Alleviated Negative Plant-Soil Feedback by Modifying Soil Microbiome
    Article Snippet: .. The PCR mixtures contain LightCycler 480 SYBR Green I Master (Roche Diagnostics Gmbh, Germany) 10 μL, ddH2 O 3 μL, forward primer (10 μM) 1 μL, reverse primer (10 μM) 1 μL, and template DNA 5 μL. .. Statistical Analysis The significant differences in the seed germination and seedling survival rate, chemical properties of soils, disease index and the incidence of disease were calculated at the 5% level using one-way analysis of variance (ANOVA) by SPSS version 18.0 software (SPSS Inc. Chicago, IL, United States).

    Article Title: Impact of Chemical and Alternative Fungicides Applied to Grapevine cv Nebbiolo on Microbial Ecology and Chemical-Physical Grape Characteristics at Harvest
    Article Snippet: .. PCR mixtures (25 μL) were prepared using 12.5 μL of the 2X Kapa HiFi HotStart ReadyMix Taq (Roche, Milan, Italy), 1 μM of each primer, 2.5 μL of DNA template, and PCR-grade water. ..

    Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells
    Article Snippet: .. The PCR mixtures were then cleaned-up using Roche PCR purification kit (Roche 11732676001). .. DNA was further purified with Ampure XP beads (Agencourt A63882).

    Article Title: B7 Costimulation Molecules Expressed from the Herpes Simplex Virus 2 Genome Rescue Immune Induction in B7-Deficient Mice ▿
    Article Snippet: .. One-tenth of the reverse transcription reaction was added to PCR mixtures containing high-fidelity Taq buffer with MgCl2 (Roche), 0.2 mM deoxynucleoside triphosphates, 50 pmol each primer, and 1 U Taq polymerase. .. Each reaction mixture was subjected to a hot start at 94°C for 4 min, followed by denaturation at 94°C for 50 s, annealing at 55°C for 50 s, and extension at 72°C for 60 s. Thirty cycles of amplification were followed by a final extension at 72°C for 5 min. PCR products were visualized on 2% agarose gels stained with ethidium bromide.

    Article Title: Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
    Article Snippet: .. PCR mixtures consisted of 5-μL LightCycler 480 SYBR Green I Master (Roche Applied Sciences, Indianapolis, IN, USA), 2.5 μL each primer of corresponding concentration 5 μM (tetA, B, C, L, M, O, X, sulI , II ), 2 μM (tetG, K, sulIII ), 1 μM (tetQ ) and 1 μL DNA template of 5 ng/μL. .. In each run, 1 μL microbial DNA-free water as negative control was included.

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. All PCR mixtures contained PCR buffer (final concentration, 10 mM Tris–HCl (pH 9.0), 50 mM KCl, 2 mM MgCl2 , and 0.1% Triton X-100), 250 μM deoxynucleoside triphosphate (Roche Molecular Biochemicals), 0.5 μM of each PCR primer, 0.5× SYBR Green I (Molecular Probes, Carlsbad, CA), 5% DMSO, and 1 unit of Taq DNA polymerase (Promega) with 2 μl of cDNA in a 25-μl final volume reaction mix. ..

    SYBR Green Assay:

    Article Title: Biochar Application Alleviated Negative Plant-Soil Feedback by Modifying Soil Microbiome
    Article Snippet: .. The PCR mixtures contain LightCycler 480 SYBR Green I Master (Roche Diagnostics Gmbh, Germany) 10 μL, ddH2 O 3 μL, forward primer (10 μM) 1 μL, reverse primer (10 μM) 1 μL, and template DNA 5 μL. .. Statistical Analysis The significant differences in the seed germination and seedling survival rate, chemical properties of soils, disease index and the incidence of disease were calculated at the 5% level using one-way analysis of variance (ANOVA) by SPSS version 18.0 software (SPSS Inc. Chicago, IL, United States).

    Article Title: Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
    Article Snippet: .. PCR mixtures consisted of 5-μL LightCycler 480 SYBR Green I Master (Roche Applied Sciences, Indianapolis, IN, USA), 2.5 μL each primer of corresponding concentration 5 μM (tetA, B, C, L, M, O, X, sulI , II ), 2 μM (tetG, K, sulIII ), 1 μM (tetQ ) and 1 μL DNA template of 5 ng/μL. .. In each run, 1 μL microbial DNA-free water as negative control was included.

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. All PCR mixtures contained PCR buffer (final concentration, 10 mM Tris–HCl (pH 9.0), 50 mM KCl, 2 mM MgCl2 , and 0.1% Triton X-100), 250 μM deoxynucleoside triphosphate (Roche Molecular Biochemicals), 0.5 μM of each PCR primer, 0.5× SYBR Green I (Molecular Probes, Carlsbad, CA), 5% DMSO, and 1 unit of Taq DNA polymerase (Promega) with 2 μl of cDNA in a 25-μl final volume reaction mix. ..

    Purification:

    Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells
    Article Snippet: .. The PCR mixtures were then cleaned-up using Roche PCR purification kit (Roche 11732676001). .. DNA was further purified with Ampure XP beads (Agencourt A63882).

    Concentration Assay:

    Article Title: Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
    Article Snippet: .. PCR mixtures consisted of 5-μL LightCycler 480 SYBR Green I Master (Roche Applied Sciences, Indianapolis, IN, USA), 2.5 μL each primer of corresponding concentration 5 μM (tetA, B, C, L, M, O, X, sulI , II ), 2 μM (tetG, K, sulIII ), 1 μM (tetQ ) and 1 μL DNA template of 5 ng/μL. .. In each run, 1 μL microbial DNA-free water as negative control was included.

    Article Title: ESE-1 Is a Potent Repressor of Type II Collagen Gene (COL2A1) Transcription In Human Chondrocytes
    Article Snippet: .. All PCR mixtures contained PCR buffer (final concentration, 10 mM Tris–HCl (pH 9.0), 50 mM KCl, 2 mM MgCl2 , and 0.1% Triton X-100), 250 μM deoxynucleoside triphosphate (Roche Molecular Biochemicals), 0.5 μM of each PCR primer, 0.5× SYBR Green I (Molecular Probes, Carlsbad, CA), 5% DMSO, and 1 unit of Taq DNA polymerase (Promega) with 2 μl of cDNA in a 25-μl final volume reaction mix. ..

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    Roche telomerase activity assays cancer cells
    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding <t>telomerase</t> activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.
    Telomerase Activity Assays Cancer Cells, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Roche plasma hbv dna
    Comparison of <t>HBV</t> replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV <t>DNA</t> from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p
    Plasma Hbv Dna, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche p malariae strains greece
    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. <t>malariae</t> (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control
    P Malariae Strains Greece, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gfp  (Roche)
    92
    Roche gfp
    Colocalization and effects of direct NPFFR2 signalling on <t>NPY</t> neurons. a Representative image of <t>GFP</t> expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p
    Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: STAT3 binding to promoter region hTERT were inhibited by the resveratrol and 5-FU alone and in combination and corresponding telomerase activities were decreased upon the combination treatments Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. (A) HCT116 was treated with 5-FU and resveratrol alone and in combination and applied to ChIP assay. STAT3 binding was quantified by the ChIP band intensity relative to untreated control, which was set as 1.0. (B) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. (C) DLD1 was treated with 5-FU (10 μM) alone and associated with 25 μM resveratrol combination, then applied to ChIP assay to quantify the STAT3 binding. (D) DLD1 was treated with 5-FU and resveratrol alone and in combination and applied to TRAP-PCR-Elisa assay to quantify telomerase activities. Error bars represent standard deviation. Telomerase activities were measured three times independently.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Standard Deviation

    Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Journal: Oncotarget

    Article Title: Combination of resveratrol and 5-flurouracil enhanced anti-telomerase activity and apoptosis by inhibiting STAT3 and Akt signaling pathways in human colorectal cancer cells

    doi: 10.18632/oncotarget.25993

    Figure Lengend Snippet: Schematic representation of combination treatment effects with 5-FU and resveratrol in colorectal cancer cells The combined treatments with 5-FU and resveratrol inhibit Akt and STAT3 signaling pathways in colorectal cancer. Akt plays a key role in cell proliferation and survival and STAT3 is key transcription factor for telomerase and other target genes involved in immune response and potential stem-like traits. Our model suggests that combined treatments of 5-FU and resveratrol drive apoptosis by inhibiting Akt and STAT3, concurrently decreasing telomerase activity and downregulating target genes of STAT3 leading to re-sensitization of colorectal cancer to chemotherapy.

    Article Snippet: Telomerase activity assays Cancer cells were processed according to the manufacturer's protocol for the TeloTAGGG Telomerase PCR ELISA kit (Roche, Orange, CA.

    Techniques: Activity Assay

    Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Comparison of HBV replication between wild-type (WT) and mutants SPII in HepG2 cells. The p1.2/PC-based SPII WT and mutant plasmids were transfected into HepG2 cells that were harvested 72 hours after transfection. Total RNA was isolated for detection of HBV total RNA ( A ) and pregenomic RNA (pgRNA) ( B ) using RT-qPCR. HBV DNA from cell supernatant ( C ) and cell lysate ( D ) was measured by qPCR. Data shown as fold change relative to WT. Mann–Whitney U tests were employed. ** p

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, Transfection, Isolation, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Journal: Viruses

    Article Title: Naturally Occurring Mutations within HBV Surface Promoter II Sequences Affect Transcription Activity, HBsAg and HBV DNA Levels in HBeAg-Positive Chronic Hepatitis B Patients

    doi: 10.3390/v11010078

    Figure Lengend Snippet: Scatter plots presenting levels of HBsAg ( A )/HBV DNA ( B ) for 87 chronic hepatitis B (CHB) C2-subgenotype patients without/with G120A mutation. According to Mann–Whitney U tests, the virological characteristics between the two groups were statistically different ( p = 0.0040 for HBsAg and p = 0.0237 for HBV DNA, respectively).

    Article Snippet: The detection limit of plasma HBV DNA by the Roche TaqMan48 automatic florescence quantitative polymerase chain reaction (qPCR) kit was 12 IU/mL.

    Techniques: Mutagenesis, MANN-WHITNEY

    Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using MSP1 19 proteins from four Plasmodium species. A combined dilution (1:400 of each serum) containing sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) was incubated with the indicated concentrations of the MSP1 19 competitor protein for 1 h at room temperature. Competitor proteins used were: a P. falciparum MSP1 19 ; b P. malariae MSP1 19 ; c P. ovale MSP1 19 ; d P. vivax MSP1 19 . Multiplex bead assays were performed as described in “ Methods ” and the multiplex response in MFI-bg units are plotted versus the competitor concentration. Multiplex responses are presented as a percentage of the assay results for the PBS control

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Antibody competition titration assays using homologous MSP1 19 proteins. Dilutions (1:400) of P. falciparum Lot 6 defined human serum or of sera from chimpanzees experimentally infected with either P. malariae (Klimatis), P. ovale (Alpert) or P. vivax (Duff) were incubated with the indicated concentrations of the homologous MSP1 19 competitor protein for 1 h at room temperature. Multiplex bead assays were performed as described in “ Methods ”, and the multiplex responses in MFI-bg units are plotted versus the competitor concentration

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Titration, Infection, Incubation, Multiplex Assay, Concentration Assay

    Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Journal: Malaria Journal

    Article Title: Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP119 subunit proteins in multiplexed serologic assays

    doi: 10.1186/s12936-018-2566-0

    Figure Lengend Snippet: Alignment of predicted Plasmodium spp. MSP1 19 protein sequences using COBALT [ 61 ]. Residues in the P. malariae sequence that differ from the Cameroon sequence of Birkenmeyer et al. [ 38 ] are shaded. Predicted protein sequences resulting from the oligonucleotides used in PCR amplification are underlined. The positions of residues conserved among all the presented MSP1 19 protein sequences are indicated in the consensus with divergent residues indicated by a dot. GenBank accession numbers are MH577181, P. ovale Nigeria I strain; MH577182, P. malariae China I strain; MH577183, P. malariae Greece I strain; MH577184, P. malariae Uganda I strain; and MH577185, P. malariae Guyana strain

    Article Snippet: Comparison of Plasmodium malariae MSP119 sequences from other geographic locations Ten nanograms of DNA from P. malariae strains Greece I, Guyana, and Uganda I were PCR amplified using the forward and reverse long deoxyoligonucleotides described above and the Expand High Fidelity PCR system (Roche Applied Science, Indianapolis, IN, USA).

    Techniques: Sequencing, Polymerase Chain Reaction, Amplification

    Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Journal: Nature Communications

    Article Title: Diet-induced adaptive thermogenesis requires neuropeptide FF receptor-2 signalling

    doi: 10.1038/s41467-018-06462-0

    Figure Lengend Snippet: Colocalization and effects of direct NPFFR2 signalling on NPY neurons. a Representative image of GFP expression in the Arc of a NPY-TRAP mouse brain. Scale bar = 100 µm. b, c Quantification of the expression of Npy and Npffr2 mRNA in the input and immunoprecipitated (IP) RNA isolated from the Arc of NPY-TRAP ( n = 10), Ins-TRAP ( n = 3) and WT-TRAP ( n = 3) mice. One-way ANOVA was used to determine difference between groups. ∗∗ p

    Article Snippet: RT-qPCR using primers for Npy , GFP and Npffr2 was carried out in samples prior (input) and after the immunoprecipitation in at least triplicates from 1:5 dilution cDNA from each sample using the LightCycler® (LightCycler® 480 Real-Time PCR system, Roche Applied Science, Germany), SYBR Green I (Molecular Probes) and Platinum Taq DNA Polymerase (Invitrogen).

    Techniques: Expressing, Immunoprecipitation, Isolation, Mouse Assay