pcr mixture  (Roche)


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    Structured Review

    Roche pcr mixture
    Sensitivity and specificity of the <t>LightCycler</t> real-time <t>PCR</t> assays for detection of target virulence genes in purified sDEC cultures.
    Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture/product/Roche
    Average 94 stars, based on 403 article reviews
    Price from $9.99 to $1999.99
    pcr mixture - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Comparison between O Serotyping Method and Multiplex Real-Time PCR To Identify Diarrheagenic Escherichia coli in Taiwan ▿"

    Article Title: Comparison between O Serotyping Method and Multiplex Real-Time PCR To Identify Diarrheagenic Escherichia coli in Taiwan ▿

    Journal:

    doi: 10.1128/JCM.00596-07

    Sensitivity and specificity of the LightCycler real-time PCR assays for detection of target virulence genes in purified sDEC cultures.
    Figure Legend Snippet: Sensitivity and specificity of the LightCycler real-time PCR assays for detection of target virulence genes in purified sDEC cultures.

    Techniques Used: Real-time Polymerase Chain Reaction, Purification

    2) Product Images from "The Lactobacillus plantarum ftsH Gene Is a Novel Member of the CtsR Stress Response Regulon ▿"

    Article Title: The Lactobacillus plantarum ftsH Gene Is a Novel Member of the CtsR Stress Response Regulon ▿

    Journal:

    doi: 10.1128/JB.01551-08

    Comparison of ftsH mRNA levels in wild-type and Δ ctsR mutant strains of L. plantarum by qRT-PCR. ftsH expression was analyzed in wild-type (filled bars) and Δ ctsR mutant (hatched bars) strains under optimal-temperature (30°C) growth
    Figure Legend Snippet: Comparison of ftsH mRNA levels in wild-type and Δ ctsR mutant strains of L. plantarum by qRT-PCR. ftsH expression was analyzed in wild-type (filled bars) and Δ ctsR mutant (hatched bars) strains under optimal-temperature (30°C) growth

    Techniques Used: Mutagenesis, Quantitative RT-PCR, Expressing

    Relative mRNA levels of L. plantarum ftsH in response to various types of stress as determined by qRT-PCR. mRNA levels were calculated relative to the transcript level detected in corresponding unstressed cultures and were normalized using ldhD as an
    Figure Legend Snippet: Relative mRNA levels of L. plantarum ftsH in response to various types of stress as determined by qRT-PCR. mRNA levels were calculated relative to the transcript level detected in corresponding unstressed cultures and were normalized using ldhD as an

    Techniques Used: Quantitative RT-PCR

    Related Articles

    Polymerase Chain Reaction:

    Article Title: F4-related mutation and expression analysis of the aminopeptidase N gene in pigs 1
    Article Snippet: .. A 10-μL PCR mix containing 0.5 U FastStart Taq DNA polymerase (Roche Diagnostics, Brussels, Belgium), 10x FastStart Buffer with 20 mM MgCl2 (Roche Diagnostics, Brussels, Belgium), 200 μM deoxynucleotide triphosphates (Bioline, London, UK), 0.5 μM primers, and the RNA template was used. ..

    Article Title: Comparison between O Serotyping Method and Multiplex Real-Time PCR To Identify Diarrheagenic Escherichia coli in Taiwan ▿
    Article Snippet: .. Each PCR mixture contained the following: 4 μl of LightCycler FastStart DNA MasterPlus HybProbe 5×-concentrated reagent (Roche Diagnostics, Penzberg, Germany), 0.5 μM each forward and reverse primers, 0.2 μM each FL and LC probes, and 5 μl of the boiled bacterial extract. .. The PCR amplification program consisted of one 10-min denaturation step at 95°C, followed by a cycling step of 45 cycles.

    Article Title: Nested PCR Amplification Secures DNA Template Quality and Quantity in Real-time mCOP-PCR Screening for SMA
    Article Snippet: .. For pre-amplification with a single PCR, 2 μL of DNA solution was added to 28 μL of PCR mixture containing 1× PCR buffer, 2 mM MgCl2 , 0.1 mM of each dNTP, 0.3 μM of each primer (R111, X7-Dra), and 1.0 U FastStart Taq DNA polymerase (Roche Applied Science, Mannheim, Germany). .. The PCR condition for each step was: (1) initial denaturation at 94°C for 7 min, (2) 35 cycles of denaturation at 94°C, annealing at 56°C for 1 min, and extension at 72°C for 1 min, (3) additional extension at 72°C for 7 min, and (4) hold at 10°C (GeneAmp PCR system 9700 Applied Bio-system, Foster city, CA, USA).

    Article Title: Single-cell RNA counting at allele- and isoform-resolution using Smart-seq3
    Article Snippet: .. The reaction was terminated by incubating at 85 degrees for 5 min. PCR preamplification was performed directly after reverse transcription by adding 6 μL of PCR mix, bringing reaction concentrations to 1x KAPA HiFi PCR buffer (contains 2mM MgCl2 at 1×) (Roche), 0.02u/μl DNA polymerase (Roche), 0.3mM dNTPs, 0.1μM Smartseq3 Forward PCR primer (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATTGCGCAATG-3’; IDT), 0.1μM Smartseq3 Reverse PCR primer (5’-ACGAGCATCAGCAGCATACGA-3’; IDT). ..

    Article Title: Splice Site Variants in the KCNQ1 and SCN5A Genes: Transcript Analysis as a Tool in Supporting Pathogenicity
    Article Snippet: .. The secondary (nested) PCR mixture comprised 1 µL of the first PCR product, together with 1× FastStart PCR buffer, 2 mM magnesium chloride, 1× GC-rich solution, 0.8 µM each of the forward and reverse inner primer , 0.4 mM dNTP, and 0.04 U FastStart Taq DNA Polymerase (Roche). .. The following cycling conditions were used: 95 °C for 4 min, 30 cycles of 94 °C for 45 s, 60 °C for 30 s, 72 °C for 2 min 45 s, and a final extension at 72 °C for 10 min. All PCR amplicons were visualized by electrophoresis in 2% EX Agarose Gels (Life Technologies) and amplicons were extracted from gels using the QIAquick Gel Extraction Kit (Qiagen) according to the manufacturer’s instructions.

    Article Title: The Lactobacillus plantarum ftsH Gene Is a Novel Member of the CtsR Stress Response Regulon ▿
    Article Snippet: .. For PCR experiments, 20 ng of genomic DNA from L. plantarum was added to a 50-μl PCR mixture and amplified with the Expand Long Template PCR system (Roche, Milan, Italy) by following the manufacturer's instructions. ..

    Article Title: The G-rich Repeats in FMR1 and C9orf72 Loci Are Hotspots for Local Unpairing of DNA
    Article Snippet: .. For ssDNA displacement at FMR1 , GC-RICH solution was added to the PCR mix, according to the manufacturer’s instructions (Roche). .. Amplified products were cloned, and single colonies were analyzed for cytosine conversions by direct sequencing (ABI 3130), using the BigDye Terminator v3.1 Cycle Sequencing Kit.

    Article Title: Spinal Muscular Atrophy: New Screening System with Real-Time mCOP-PCR and PCR-RFLP for SMN1 Deletion
    Article Snippet: .. Two μl of template solution (200~300 ng DNA from DBS) was directly added to PCR mixture (total volume, 28 μl) containing 1× PCR buffer [final concentration], 2 mM MgCl2 [final concentration], 0.1 mM of each dNTP, 0.3 μM of each primer (R111 and X7-Dra), and 1.0 U FastStart Taq DNA polymerase (Roche Applied Science, Mannheim, Germany). ..

    Nested PCR:

    Article Title: Splice Site Variants in the KCNQ1 and SCN5A Genes: Transcript Analysis as a Tool in Supporting Pathogenicity
    Article Snippet: .. The secondary (nested) PCR mixture comprised 1 µL of the first PCR product, together with 1× FastStart PCR buffer, 2 mM magnesium chloride, 1× GC-rich solution, 0.8 µM each of the forward and reverse inner primer , 0.4 mM dNTP, and 0.04 U FastStart Taq DNA Polymerase (Roche). .. The following cycling conditions were used: 95 °C for 4 min, 30 cycles of 94 °C for 45 s, 60 °C for 30 s, 72 °C for 2 min 45 s, and a final extension at 72 °C for 10 min. All PCR amplicons were visualized by electrophoresis in 2% EX Agarose Gels (Life Technologies) and amplicons were extracted from gels using the QIAquick Gel Extraction Kit (Qiagen) according to the manufacturer’s instructions.

    Amplification:

    Article Title: The Lactobacillus plantarum ftsH Gene Is a Novel Member of the CtsR Stress Response Regulon ▿
    Article Snippet: .. For PCR experiments, 20 ng of genomic DNA from L. plantarum was added to a 50-μl PCR mixture and amplified with the Expand Long Template PCR system (Roche, Milan, Italy) by following the manufacturer's instructions. ..

    Concentration Assay:

    Article Title: Spinal Muscular Atrophy: New Screening System with Real-Time mCOP-PCR and PCR-RFLP for SMN1 Deletion
    Article Snippet: .. Two μl of template solution (200~300 ng DNA from DBS) was directly added to PCR mixture (total volume, 28 μl) containing 1× PCR buffer [final concentration], 2 mM MgCl2 [final concentration], 0.1 mM of each dNTP, 0.3 μM of each primer (R111 and X7-Dra), and 1.0 U FastStart Taq DNA polymerase (Roche Applied Science, Mannheim, Germany). ..

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    Roche lc pcr master mix
    Detection of HSV <t>DNA</t> from clinical samples by LightCycler <t>PCR.</t>
    Lc Pcr Master Mix, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lc pcr master mix/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lc pcr master mix - by Bioz Stars, 2020-09
    85/100 stars
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    94
    Roche pcr mix
    Overview of single-cell RNA-sequencing in Smart-seq3. (a) Library strategy for Smart-seq3. PolyA+ RNA molecules are reverse transcribed and template switching is carried out at the 5’ end. After <t>PCR</t> <t>preamplification,</t> tagmentation via Tn5 introduces near-random cuts in the cDNA, producing 5’ UMI-tagged fragments and internal fragments spanning the whole gene body. ( b ) Gene body coverage averaged over HEK293FT (n = 96) cells sequenced with the Smart-seq3 protocol. Shown is the mean coverage of UMI reads (green) and internal reads (blue) shaded by the standard deviation. ( c ) Effect of tagmentation conditions on the fraction of UMI-containing reads (16 HEK293FT cells per condition). Left panel: varying Tn5 with constant 200 pg cDNA input. Right panel: varying cDNA input with constant 0.5ul Tn5. ( d ) Gene detection sensitivity for Smart-seq2 (44 cells) and Smart-seq3 (88 cells), downsampled to 1 million raw reads per HEK293FT cell. Shown are number of genes detected over 0 or 1 RPKM. P-value was computed as a two-sided t -test. ( e ) Reproducibility in gene expression quantification across HEKF293FT cells for Smart-seq2 (44 cells) and Smart-seq3 (88 cells) at RPKM and UMI level. Shown are adjusted r^2 for all pairwise cell to cell linear model fits in libraries downsampled to 1 million reads per cell. ( f ) Sensitivity to detect RNA molecules in Smart-seq3 shown by summarizing the number of unique error-corrected UMI sequences and genes detected per HEK293FT cell. Colors indicate the per cell downsampling depth ranging from 10.000 (n = 24 cells) to 750.000 (n = 16 cells) UMI-containing sequencing reads. ( g ) Violin plots summarizing the number of molecules detected per cell with Smart-seq2-UMI, Smart-seq3 and using smRNA-FISH for four X chromosomal genes (Hdac6, Igbp1, Mpp1 and Msl3). ( h ) Estimating the percent of smRNA-FISH molecules that were detected in cells using Smart-seq2-UMI and Smart-seq3. Shown are means and 95% confidence intervals.
    Pcr Mix, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mix/product/Roche
    Average 94 stars, based on 215 article reviews
    Price from $9.99 to $1999.99
    pcr mix - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    85
    Roche runx2 pcr mixtures
    Distribution of <t>RUNX2</t> gene expression (A) on patient’s population and statistically significant correlations between ovulation induction factors, such as number of follicles, oocytes, fertilized oocytes (B) and hormone serum levels for E2 (C) and LH (D) respectively. RUNX2 gene expression was determined by real-time <t>PCR</t> for 41 patients and categorized into two groups of patients, with expression and without expression. Expression ratios (RUNX2 copies/G6PDH copies) were used for the evaluation of RUNX2 gene expression.
    Runx2 Pcr Mixtures, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/runx2 pcr mixtures/product/Roche
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    runx2 pcr mixtures - by Bioz Stars, 2020-09
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    85
    Roche alu ltr pcr mixture
    Analysis of transcripts and nuclear HIV-1 DNA in FcγR-stimulated or control MDM. MDM (10 6 cells/well of a 12-well plate) were activated with hIgG and immediately infected with HIV-1 BaL (5 × 10 2 TCID 50 /10 6 cells) for 1 h at 37°C. Cells were lysed at the indicated time points after infection, and 5 μl of cell lysate (corresponding to approximately 20,000 cells) was analyzed by <t>PCR.</t> Primers specific for intermediate ( pol ) and late (U3/ gag ) reverse transcripts, nuclear 2LTR circles, or integrated provirus ( <t>Alu</t> -LTR) were used. PCR products were analyzed by gel electrophoresis. Four independent experiments performed with MDM from different donors showed similar PCR amplification profiles despite signal intensity variations. NI, noninfected; −, absence of hIgG; +, presence of hIgG.
    Alu Ltr Pcr Mixture, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alu ltr pcr mixture/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alu ltr pcr mixture - by Bioz Stars, 2020-09
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    Image Search Results


    Detection of HSV DNA from clinical samples by LightCycler PCR.

    Journal: BMC Microbiology

    Article Title: Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture

    doi: 10.1186/1471-2180-2-12

    Figure Lengend Snippet: Detection of HSV DNA from clinical samples by LightCycler PCR.

    Article Snippet: The LC-PCR master mix contained the following: 1× FastStart Taq DNA polymerase reaction buffer (Roche Molecular Diagnostics) which included a dNTP mix (containing dUTP instead of dTTP), 3 mM MgCl2 , 0.5 μM of each primer, 0.2 μM HSV-2 FLU and 0.4 μM HSV-2 LCR.

    Techniques: Polymerase Chain Reaction

    Overview of single-cell RNA-sequencing in Smart-seq3. (a) Library strategy for Smart-seq3. PolyA+ RNA molecules are reverse transcribed and template switching is carried out at the 5’ end. After PCR preamplification, tagmentation via Tn5 introduces near-random cuts in the cDNA, producing 5’ UMI-tagged fragments and internal fragments spanning the whole gene body. ( b ) Gene body coverage averaged over HEK293FT (n = 96) cells sequenced with the Smart-seq3 protocol. Shown is the mean coverage of UMI reads (green) and internal reads (blue) shaded by the standard deviation. ( c ) Effect of tagmentation conditions on the fraction of UMI-containing reads (16 HEK293FT cells per condition). Left panel: varying Tn5 with constant 200 pg cDNA input. Right panel: varying cDNA input with constant 0.5ul Tn5. ( d ) Gene detection sensitivity for Smart-seq2 (44 cells) and Smart-seq3 (88 cells), downsampled to 1 million raw reads per HEK293FT cell. Shown are number of genes detected over 0 or 1 RPKM. P-value was computed as a two-sided t -test. ( e ) Reproducibility in gene expression quantification across HEKF293FT cells for Smart-seq2 (44 cells) and Smart-seq3 (88 cells) at RPKM and UMI level. Shown are adjusted r^2 for all pairwise cell to cell linear model fits in libraries downsampled to 1 million reads per cell. ( f ) Sensitivity to detect RNA molecules in Smart-seq3 shown by summarizing the number of unique error-corrected UMI sequences and genes detected per HEK293FT cell. Colors indicate the per cell downsampling depth ranging from 10.000 (n = 24 cells) to 750.000 (n = 16 cells) UMI-containing sequencing reads. ( g ) Violin plots summarizing the number of molecules detected per cell with Smart-seq2-UMI, Smart-seq3 and using smRNA-FISH for four X chromosomal genes (Hdac6, Igbp1, Mpp1 and Msl3). ( h ) Estimating the percent of smRNA-FISH molecules that were detected in cells using Smart-seq2-UMI and Smart-seq3. Shown are means and 95% confidence intervals.

    Journal: bioRxiv

    Article Title: Single-cell RNA counting at allele- and isoform-resolution using Smart-seq3

    doi: 10.1101/817924

    Figure Lengend Snippet: Overview of single-cell RNA-sequencing in Smart-seq3. (a) Library strategy for Smart-seq3. PolyA+ RNA molecules are reverse transcribed and template switching is carried out at the 5’ end. After PCR preamplification, tagmentation via Tn5 introduces near-random cuts in the cDNA, producing 5’ UMI-tagged fragments and internal fragments spanning the whole gene body. ( b ) Gene body coverage averaged over HEK293FT (n = 96) cells sequenced with the Smart-seq3 protocol. Shown is the mean coverage of UMI reads (green) and internal reads (blue) shaded by the standard deviation. ( c ) Effect of tagmentation conditions on the fraction of UMI-containing reads (16 HEK293FT cells per condition). Left panel: varying Tn5 with constant 200 pg cDNA input. Right panel: varying cDNA input with constant 0.5ul Tn5. ( d ) Gene detection sensitivity for Smart-seq2 (44 cells) and Smart-seq3 (88 cells), downsampled to 1 million raw reads per HEK293FT cell. Shown are number of genes detected over 0 or 1 RPKM. P-value was computed as a two-sided t -test. ( e ) Reproducibility in gene expression quantification across HEKF293FT cells for Smart-seq2 (44 cells) and Smart-seq3 (88 cells) at RPKM and UMI level. Shown are adjusted r^2 for all pairwise cell to cell linear model fits in libraries downsampled to 1 million reads per cell. ( f ) Sensitivity to detect RNA molecules in Smart-seq3 shown by summarizing the number of unique error-corrected UMI sequences and genes detected per HEK293FT cell. Colors indicate the per cell downsampling depth ranging from 10.000 (n = 24 cells) to 750.000 (n = 16 cells) UMI-containing sequencing reads. ( g ) Violin plots summarizing the number of molecules detected per cell with Smart-seq2-UMI, Smart-seq3 and using smRNA-FISH for four X chromosomal genes (Hdac6, Igbp1, Mpp1 and Msl3). ( h ) Estimating the percent of smRNA-FISH molecules that were detected in cells using Smart-seq2-UMI and Smart-seq3. Shown are means and 95% confidence intervals.

    Article Snippet: The reaction was terminated by incubating at 85 degrees for 5 min. PCR preamplification was performed directly after reverse transcription by adding 6 μL of PCR mix, bringing reaction concentrations to 1x KAPA HiFi PCR buffer (contains 2mM MgCl2 at 1×) (Roche), 0.02u/μl DNA polymerase (Roche), 0.3mM dNTPs, 0.1μM Smartseq3 Forward PCR primer (5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGATTGCGCAATG-3’; IDT), 0.1μM Smartseq3 Reverse PCR primer (5’-ACGAGCATCAGCAGCATACGA-3’; IDT).

    Techniques: RNA Sequencing Assay, Polymerase Chain Reaction, Standard Deviation, Expressing, Sequencing, Fluorescence In Situ Hybridization

    Distribution of RUNX2 gene expression (A) on patient’s population and statistically significant correlations between ovulation induction factors, such as number of follicles, oocytes, fertilized oocytes (B) and hormone serum levels for E2 (C) and LH (D) respectively. RUNX2 gene expression was determined by real-time PCR for 41 patients and categorized into two groups of patients, with expression and without expression. Expression ratios (RUNX2 copies/G6PDH copies) were used for the evaluation of RUNX2 gene expression.

    Journal: Reproductive Biology and Endocrinology : RB & E

    Article Title: Detection of RUNX2 gene expression in cumulus cells in women undergoing controlled ovarian stimulation

    doi: 10.1186/1477-7827-10-99

    Figure Lengend Snippet: Distribution of RUNX2 gene expression (A) on patient’s population and statistically significant correlations between ovulation induction factors, such as number of follicles, oocytes, fertilized oocytes (B) and hormone serum levels for E2 (C) and LH (D) respectively. RUNX2 gene expression was determined by real-time PCR for 41 patients and categorized into two groups of patients, with expression and without expression. Expression ratios (RUNX2 copies/G6PDH copies) were used for the evaluation of RUNX2 gene expression.

    Article Snippet: RUNX2 PCR mixtures contained 5 μl cDNA, 5x master mix (LightCycler 480 Genotyping Master, Roche), 20 μM of each primer, 20 μM of each probe and PCR-grade water (LightCycler 480 Genotyping Master, Roche) to a total volume of 20 μl.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Analysis of transcripts and nuclear HIV-1 DNA in FcγR-stimulated or control MDM. MDM (10 6 cells/well of a 12-well plate) were activated with hIgG and immediately infected with HIV-1 BaL (5 × 10 2 TCID 50 /10 6 cells) for 1 h at 37°C. Cells were lysed at the indicated time points after infection, and 5 μl of cell lysate (corresponding to approximately 20,000 cells) was analyzed by PCR. Primers specific for intermediate ( pol ) and late (U3/ gag ) reverse transcripts, nuclear 2LTR circles, or integrated provirus ( Alu -LTR) were used. PCR products were analyzed by gel electrophoresis. Four independent experiments performed with MDM from different donors showed similar PCR amplification profiles despite signal intensity variations. NI, noninfected; −, absence of hIgG; +, presence of hIgG.

    Journal: Journal of Virology

    Article Title: Fc? Receptor-Mediated Suppression of Human Immunodeficiency Virus Type 1 Replication in Primary Human Macrophages

    doi: 10.1128/JVI.77.7.4081-4094.2003

    Figure Lengend Snippet: Analysis of transcripts and nuclear HIV-1 DNA in FcγR-stimulated or control MDM. MDM (10 6 cells/well of a 12-well plate) were activated with hIgG and immediately infected with HIV-1 BaL (5 × 10 2 TCID 50 /10 6 cells) for 1 h at 37°C. Cells were lysed at the indicated time points after infection, and 5 μl of cell lysate (corresponding to approximately 20,000 cells) was analyzed by PCR. Primers specific for intermediate ( pol ) and late (U3/ gag ) reverse transcripts, nuclear 2LTR circles, or integrated provirus ( Alu -LTR) were used. PCR products were analyzed by gel electrophoresis. Four independent experiments performed with MDM from different donors showed similar PCR amplification profiles despite signal intensity variations. NI, noninfected; −, absence of hIgG; +, presence of hIgG.

    Article Snippet: The Alu -LTR PCR mixture contained 5 μl of lysate, 5 μl of 10× PCR buffer (Roche Diagnostics GmbH), 1.75 μl of 10 nM dNTP, 1.5 μl each of sense and antisense primers (from a 10 μM solution), and 0.75 μl of Long Expand Template Taq DNA polymerase (Roche Diagnostics GmbH).

    Techniques: Infection, Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Amplification