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tiangen biotech co pcr mix
Pcr Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr mix/product/tiangen biotech co
Average 92 stars, based on 28 article reviews
Price from $9.99 to $1999.99
pcr mix - by Bioz Stars, 2020-10
92/100 stars

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Polymerase Chain Reaction:

Article Title: Interleukin-27 rs153109 polymorphism and the risk of non-small-cell lung cancer in a Chinese population
Article Snippet: .. A 20-μL volume of the PCR mixture contained 50–150 ng genomic DNA and 10 μL of 2× PCR mix (Tiangen Biotech[Beijing]Co., Ltd.). .. For PCR amplification, an initial denaturation at 94°C for 5 minutes was followed by 36 cycles at 94°C for 30 seconds, at 64°C for 30 seconds, at 72°C for 30 seconds, and a final extension at 72°C for 10 minutes.

Article Title: p33ING1b methylation in fecal DNA as a molecular screening tool for colorectal cancer and precancerous lesions
Article Snippet: .. Briefly, 3 μ l bisulfite-modified DNA was added in a final volume of 25 μ l PCR mixture containing 2.5 μ l 10X PCR buffer, 2 μ l deoxynucleotide triphosphates (2.5 mmol/l), 0.5 μ l each nested primer (10 μ mol/l) and 1 unit Long Taq polymerase (Tiangen Biotech (Beijing) Co., Ltd., Beijing, China). .. The PCR amplification protocol for stage 1 was as follows: 94°C for 4 min, followed by 35 cycles at 94°C for 30 sec, specific annealing temperature 53°C for 30 sec and 72°C for 30 sec, and finally 72°C for 5 min. For stage 2 amplifications [PCR with the methylation-specific primers (Sangon Biotech (Shanghai) Co. Ltd., Shanghai, China)] 1 μ l first-step PCR products was added in a final volume of 25 μ l PCR mixture: 2.5 μ l 10X PCR buffer, 2 μ l deoxynucleotide triphosphates (2.5 mmol/l), 0.5 μ l each MSP primer (10 μ mol/l) and 1 unit Taq polymerase (Takara, Dalian, China).

Article Title: High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers
Article Snippet: .. PCR amplification was done in a T-100 Thermal Cycler (Bio-Rad, USA) and reactions consisting of 100 ng template DNA, 25 μL 2 × PCR Mix (Tiangen Biotech, China), and 0.5 μM of each primer in a final reaction volume of 50 μL. .. The PCR cycling conditions were as follows: initial denaturation at 94 °C for 3 min, followed by 40 cycles at 94 °C for 45 s, 58 °C for 45 s, 72 °C for 60 s, and a final extension at 72 °C for 10 min.

Article Title: High prevelance of human parvovirus infection in patients with malignant tumors
Article Snippet: .. The extracted DNA (2.5 μl) was added to the PCR mixture containing 2.5 μl of 10X reaction buffer (Tiangen Biotech, Beijing Co. Ltd, China), 200 μM of each dATP, dCTP, dGTP and dTTP, 12.5 pmol of each primer and 1.25 units of Taq polymerase (Tiangen Biotech). .. The nested-PCR of B19 was performed under the following conditions: denaturation at 94°C for 4 min, 30 cycles of 94°C for 30 sec, annealing at 56°C for 30 sec and 72°C for 1 min and elongation at 72°C for 10 min.

Article Title: IL-27 rs153109 polymorphism increases the risk of colorectal cancer in Chinese Han population
Article Snippet: .. A 20 μL PCR mixture contained 50–150 ng of genomic DNA and 10 μL 2× PCR mix (Tiangen Biotech). .. For PCR amplification, an initial denaturation at 94°C for 5 minutes was followed by 36 cycles at 94°C for 30 seconds, at 64°C for 30 seconds, at 72°C for 30 seconds, and a final extension at 72°C for 10 minutes.

Article Title: A novel splice site mutation in the COL4A5 gene in a Chinese female patient with rare ocular abnormalities
Article Snippet: .. The PCR mixture (per 25 μl) contained cDNA template 1 μl, 2× Taq plus Master Mix (Tiangen Biotech Co., Ltd., Beijing, China) 12.5 μl, and 5 pmol/μl of each primer 1μl. .. The PCR parameters were optimized as follows: an initial denaturation at 94 °C for 7 min, 35 cycles of denaturation (94 °C for 30 s), annealing (58 °C for 30 s), elongation (72 °C for 45 s), and a final elongation for 7 min at 72 °C.

Article Title: Study of a common azo food dye in mice model: Toxicity reports and its relation to carcinogenicity, et al. Study of a common azo food dye in mice model: Toxicity reports and its relation to carcinogenicity
Article Snippet: .. Each PCR mixture consisted of 1× Taq DNA polymerase buffer, 0.5 μl of each primer from 10 mM stock, 0.5 μl of dNTPs mix (10 mM each), and 0.25 U of Tiangen Taq platinum DNA polymerase (Tiangen Biotech Co. Ltd., Beijing, China) in a Astec‐482 (Astec, Japan) thermal cycler. .. The cycling condition was initial PCR activation step of 6 min at 94°C, followed by 35 cycles of a 45‐s denaturation step at 94°C, a 45‐s annealing step at 52°C (for GAPDH and PARP), 60‐s synthesis step at 72°C, and a final extension of 72°C for 10 min.

Article Title: Molecular Sex Identification in the Hardy Rubber Tree (Eucommia ulmoides Oliver) via ddRAD Markers
Article Snippet: .. We used the volume of 25 μ L for PCR amplification reaction: 12.5 μL PCR mix (Tiangen Biotech, Beijing, China), 2.0 μ L random decamer primers (25 μ mol·μ L−1 ), 0.5 μ L genomic DNA (100 ng·μ L−1 ), and 10.0 μ L ddH2O. .. The following PCR profile was used: an initial denaturation at 94°C for 3 min followed by 30 cycles of denaturation (30 s at 94°C), annealing (30 s at 59°C) and extension (1 min at 72°C), and a final extension 72°C for 5 min.

Amplification:

Article Title: High-density genetic map construction and mapping of the homologous transformation sterility gene (hts) in wheat using GBS markers
Article Snippet: .. PCR amplification was done in a T-100 Thermal Cycler (Bio-Rad, USA) and reactions consisting of 100 ng template DNA, 25 μL 2 × PCR Mix (Tiangen Biotech, China), and 0.5 μM of each primer in a final reaction volume of 50 μL. .. The PCR cycling conditions were as follows: initial denaturation at 94 °C for 3 min, followed by 40 cycles at 94 °C for 45 s, 58 °C for 45 s, 72 °C for 60 s, and a final extension at 72 °C for 10 min.

Article Title: Molecular Sex Identification in the Hardy Rubber Tree (Eucommia ulmoides Oliver) via ddRAD Markers
Article Snippet: .. We used the volume of 25 μ L for PCR amplification reaction: 12.5 μL PCR mix (Tiangen Biotech, Beijing, China), 2.0 μ L random decamer primers (25 μ mol·μ L−1 ), 0.5 μ L genomic DNA (100 ng·μ L−1 ), and 10.0 μ L ddH2O. .. The following PCR profile was used: an initial denaturation at 94°C for 3 min followed by 30 cycles of denaturation (30 s at 94°C), annealing (30 s at 59°C) and extension (1 min at 72°C), and a final extension 72°C for 5 min.

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    tiangen biotech co pcr master mix
    BstUI <t>PCR‐RFLP</t> analysis of the PLIN1 biallelic polymorphism. M, <t>DNA</t> ladder; Lane1, AA; Lane2, AG; Lane3, GG.
    Pcr Master Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr master mix/product/tiangen biotech co
    Average 94 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    pcr master mix - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    92
    tiangen biotech co quantitative rt pcr qrt pcr fastquant rt super mix
    Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by <t>qRT-PCR.</t> Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p
    Quantitative Rt Pcr Qrt Pcr Fastquant Rt Super Mix, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative rt pcr qrt pcr fastquant rt super mix/product/tiangen biotech co
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative rt pcr qrt pcr fastquant rt super mix - by Bioz Stars, 2020-10
    92/100 stars
      Buy from Supplier

    Image Search Results


    BstUI PCR‐RFLP analysis of the PLIN1 biallelic polymorphism. M, DNA ladder; Lane1, AA; Lane2, AG; Lane3, GG.

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: An Improved PCR‐RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene

    doi: 10.1002/jcla.21968

    Figure Lengend Snippet: BstUI PCR‐RFLP analysis of the PLIN1 biallelic polymorphism. M, DNA ladder; Lane1, AA; Lane2, AG; Lane3, GG.

    Article Snippet: PCR was performed in a total volume of 25 μL containing 100 ng of genomic DNA, 1× PCR master Mix (Tiangen), and 5 pmol of each primer.

    Techniques: Polymerase Chain Reaction

    Examples of DNA sequencing of PCR product of PLIN1 gene.

    Journal: Journal of Clinical Laboratory Analysis

    Article Title: An Improved PCR‐RFLP Assay for the Detection of a Polymorphism rs2289487 of PLIN1 Gene

    doi: 10.1002/jcla.21968

    Figure Lengend Snippet: Examples of DNA sequencing of PCR product of PLIN1 gene.

    Article Snippet: PCR was performed in a total volume of 25 μL containing 100 ng of genomic DNA, 1× PCR master Mix (Tiangen), and 5 pmol of each primer.

    Techniques: DNA Sequencing, Polymerase Chain Reaction

    Effect of daphnetin and 5-aza-dc on expression of DR3, PDCD5, FasL, p53 (A) and DNMT1, DNMT3a, DNMT3b (B) in CIA rat synovial cells. Four experimental groups consisting of untreated cell control, or treated with 5-aza-dc at 20 μM, daphnetin at 40 μg/mL, or combination of 5-aza-dc (20 μM) and daphnetin (40 μg/mL). Cells were cultured, treated and harvested as described in Matreials and Methods. Total RNA was extracted and cDNA was synthesized. After reverse transcription, cDNA was used for real time-PCR. Relative quantification of gene expression was performed by the 2-∆∆Ct method. The results show the mean ± S.D. of six independent experiments. ▲P

    Journal: Journal of Translational Medicine

    Article Title: Therapeutic effect of daphnetin on the autoimmune arthritis through demethylation of proapoptotic genes in synovial cells

    doi: 10.1186/s12967-014-0287-x

    Figure Lengend Snippet: Effect of daphnetin and 5-aza-dc on expression of DR3, PDCD5, FasL, p53 (A) and DNMT1, DNMT3a, DNMT3b (B) in CIA rat synovial cells. Four experimental groups consisting of untreated cell control, or treated with 5-aza-dc at 20 μM, daphnetin at 40 μg/mL, or combination of 5-aza-dc (20 μM) and daphnetin (40 μg/mL). Cells were cultured, treated and harvested as described in Matreials and Methods. Total RNA was extracted and cDNA was synthesized. After reverse transcription, cDNA was used for real time-PCR. Relative quantification of gene expression was performed by the 2-∆∆Ct method. The results show the mean ± S.D. of six independent experiments. ▲P

    Article Snippet: Real-time PCR was performed in the ABI 7500 sequence detection system (Applied Biosystems) with a reaction mixture that consisted of SYBR Green 2 × PCR Master Mix (Tiangen biotech co., LTD., Beijing, China), cDNA template, forward primer and reverse primer.

    Techniques: Expressing, Cell Culture, Synthesized, Real-time Polymerase Chain Reaction

    Construction and assay of pEGFP-C1/HHEX. (A) HHEX cDNA electrophoresis products of PCR (1: DNA marker III, 2: HHEX cDNA, 3: negative control); (B) pGM-T/HHEX digestion products (1: DNA marker III, 2: pGM-T/HHEXdigestion products); (C) pEGFP-C1/HHEX digestion products (1: 1Kb DNA marker; 2: pEGFP-C1 digestion products; 3: pEGFP-C1/HHEX digestion products); (D) Normal control; (E) pEGFP-C1; (F) pEGFP-C1/HHEX.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: HHEX: A Crosstalker between HCMV Infection and Proliferation of VSMCs

    doi: 10.3389/fcimb.2016.00169

    Figure Lengend Snippet: Construction and assay of pEGFP-C1/HHEX. (A) HHEX cDNA electrophoresis products of PCR (1: DNA marker III, 2: HHEX cDNA, 3: negative control); (B) pGM-T/HHEX digestion products (1: DNA marker III, 2: pGM-T/HHEXdigestion products); (C) pEGFP-C1/HHEX digestion products (1: 1Kb DNA marker; 2: pEGFP-C1 digestion products; 3: pEGFP-C1/HHEX digestion products); (D) Normal control; (E) pEGFP-C1; (F) pEGFP-C1/HHEX.

    Article Snippet: Plasmid extraction kit and DNA gel extraction kit were supplied by OMEGA, USA. pGM-T vector kit and PCR master mix were from Tiangen (Beijing) Co., Ltd. TRIzol and Lipofectamine LTX and PLUS reagents were from Life Technologies, USA. cDNA first strand synthesis kit was from Toyobo (Shanghai) biological Technology Co. CCK 8 cell proliferation test kit was supplied by Dongren chemical technology (Shanghai) Co., LTD. Hoechst33342/PI double dye cell apoptosis detection kit was from Beibo (Shanghai) biological technology Co., LTD.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Marker, Negative Control

    Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by qRT-PCR. Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Molecular and Bioinformatics Analyses Identify 7 Circular RNAs Involved in Regulation of Oncogenic Transformation and Cell Proliferation in Human Bladder Cancer

    doi: 10.12659/MSM.908837

    Figure Lengend Snippet: Validation of the differentially expressed circRNAs. To confirm the findings from the microarray analysis, the relative expression of circRNAs was measured by qRT-PCR. Five out of the 7 significantly differentially expressed circRNAs were measured. Note that some circRNAs have not yet been named yet, and are tentatively named here using the “chromosome: start_position” format. * p

    Article Snippet: Quantitative RT-PCR (qRT-PCR) FastQuant RT Super Mix (Tiangen, Beijing, China) was used to synthesize cDNA templates.

    Techniques: Microarray, Expressing, Quantitative RT-PCR