Structured Review

TaKaRa pcr mix
Exon/intron structure and alternative mRNA transcripts of mouse GAD1 gene. The new arrangement of mouse GAD1 exons and introns as determined after the analysis of genomic and <t>cDNA</t> sequence data using bioinformatics, 3′-RACE, <t>RT-PCR,</t> cloning and sequencing. Exons are shown as numbered boxes (red numbers represent alternatively spliced exons) and introns as lines. Large boxes indicate the coding DNA sequence and the small boxes the 5′- and 3′-untranslated regions. The red arrowheads are showing the locations of the alternative promoters. The schematic representation of GAD1 splicing isoforms in relation to the gene is shown below the gene structure. The length in base pairs and the position of start and stop codons are indicated above each isoform. Diagrams are not to scale.
Pcr Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr mix/product/TaKaRa
Average 99 stars, based on 168 article reviews
Price from $9.99 to $1999.99
pcr mix - by Bioz Stars, 2020-09
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Images

1) Product Images from "Glutamic acid decarboxylase 1 alternative splicing isoforms: characterization, expression and quantification in the mouse brain"

Article Title: Glutamic acid decarboxylase 1 alternative splicing isoforms: characterization, expression and quantification in the mouse brain

Journal: BMC Neuroscience

doi: 10.1186/1471-2202-15-114

Exon/intron structure and alternative mRNA transcripts of mouse GAD1 gene. The new arrangement of mouse GAD1 exons and introns as determined after the analysis of genomic and cDNA sequence data using bioinformatics, 3′-RACE, RT-PCR, cloning and sequencing. Exons are shown as numbered boxes (red numbers represent alternatively spliced exons) and introns as lines. Large boxes indicate the coding DNA sequence and the small boxes the 5′- and 3′-untranslated regions. The red arrowheads are showing the locations of the alternative promoters. The schematic representation of GAD1 splicing isoforms in relation to the gene is shown below the gene structure. The length in base pairs and the position of start and stop codons are indicated above each isoform. Diagrams are not to scale.
Figure Legend Snippet: Exon/intron structure and alternative mRNA transcripts of mouse GAD1 gene. The new arrangement of mouse GAD1 exons and introns as determined after the analysis of genomic and cDNA sequence data using bioinformatics, 3′-RACE, RT-PCR, cloning and sequencing. Exons are shown as numbered boxes (red numbers represent alternatively spliced exons) and introns as lines. Large boxes indicate the coding DNA sequence and the small boxes the 5′- and 3′-untranslated regions. The red arrowheads are showing the locations of the alternative promoters. The schematic representation of GAD1 splicing isoforms in relation to the gene is shown below the gene structure. The length in base pairs and the position of start and stop codons are indicated above each isoform. Diagrams are not to scale.

Techniques Used: Sequencing, Reverse Transcription Polymerase Chain Reaction, Clone Assay

Expression analysis of mouse GAD1 splicing isoforms in adult brain. (A) RT-PCR analysis of the expression of GAD1 mRNA splicing isoforms in adult mouse brain by using a forward primer either in Exon1 or Exon2 and a specific reverse primer for each transcript. (B) Analysis of the expression of Isoforms 1 to 6 by using the PCR product of lanes Exon1 and Exon2 in (A) as template for nested PCR with specific primers for each isoform. (C) Gel electrophoresis of Isoforms 3/4 and 5/6 , amplified from mouse brain cDNA using specific forward primer and reverse primer, close to the position of the polyadenylation signal. Plasmid containing Isoforms 1/2 was used as a negative control with the primers for Isoforms 3/4 and 5/6 and as a positive control with primers amplifying the 3′-end of Isoforms 1/2 . (D) Southern blotting of the gel in (C) . The membrane was probed with Probe-GAD1 against 3′-region of Isoforms 1 and 2 . Ma, marker λ/HindIII-ϕX174/HaeIII.
Figure Legend Snippet: Expression analysis of mouse GAD1 splicing isoforms in adult brain. (A) RT-PCR analysis of the expression of GAD1 mRNA splicing isoforms in adult mouse brain by using a forward primer either in Exon1 or Exon2 and a specific reverse primer for each transcript. (B) Analysis of the expression of Isoforms 1 to 6 by using the PCR product of lanes Exon1 and Exon2 in (A) as template for nested PCR with specific primers for each isoform. (C) Gel electrophoresis of Isoforms 3/4 and 5/6 , amplified from mouse brain cDNA using specific forward primer and reverse primer, close to the position of the polyadenylation signal. Plasmid containing Isoforms 1/2 was used as a negative control with the primers for Isoforms 3/4 and 5/6 and as a positive control with primers amplifying the 3′-end of Isoforms 1/2 . (D) Southern blotting of the gel in (C) . The membrane was probed with Probe-GAD1 against 3′-region of Isoforms 1 and 2 . Ma, marker λ/HindIII-ϕX174/HaeIII.

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Nested PCR, Nucleic Acid Electrophoresis, Amplification, Plasmid Preparation, Negative Control, Positive Control, Southern Blot, Marker

Expression of GAD1 mRNA splicing isoforms and GAD2 in different mouse tissues and during development. The expression level of Isoforms 1/2 (A) , GAD2 (B) , Isoforms 3/4 (C) , Isoforms 5/6 (D) , Isoforms 7/8 (E) , Isoforms 9/10 (F) are compared by PCR amplification using mouse Multiple Tissue cDNA Panel I and cDNA from pancreas, small intestine, and large intestine as cDNA template. (G) Control PCR reaction to verify the specificity of the primers for Isoforms 1/2, 3/4 and 5/6 . In the control reaction each primer pair was tested with a plasmid containing each full length insert as a template. The template in lanes 1, 4 and 7 was plasmid containing Isoforms 1/2 as template; lanes 2, 5 and 8, plasmid containing Isoforms 5/6 ; and lanes 3, 6, 9 plasmid containing Isoforms 3/4 . Lanes (Ht) heart; (Br) brain; (Sp) spleen; (L) lung; (Li) liver; (Ms) muscle; (K) kidney; (Ts) testis; (E7) 7-day embryo; (E11) 11-day embryo; (E15) 15-day embryo; (E17) 17-day embryo; (P) pancreas; (SI) small intestine; (LI) large intestine; (N) no template control; (−) plasmid containing Isoforms 1/2 is used as template for the amplification; and Ma, marker ϕX174/HaeIII.
Figure Legend Snippet: Expression of GAD1 mRNA splicing isoforms and GAD2 in different mouse tissues and during development. The expression level of Isoforms 1/2 (A) , GAD2 (B) , Isoforms 3/4 (C) , Isoforms 5/6 (D) , Isoforms 7/8 (E) , Isoforms 9/10 (F) are compared by PCR amplification using mouse Multiple Tissue cDNA Panel I and cDNA from pancreas, small intestine, and large intestine as cDNA template. (G) Control PCR reaction to verify the specificity of the primers for Isoforms 1/2, 3/4 and 5/6 . In the control reaction each primer pair was tested with a plasmid containing each full length insert as a template. The template in lanes 1, 4 and 7 was plasmid containing Isoforms 1/2 as template; lanes 2, 5 and 8, plasmid containing Isoforms 5/6 ; and lanes 3, 6, 9 plasmid containing Isoforms 3/4 . Lanes (Ht) heart; (Br) brain; (Sp) spleen; (L) lung; (Li) liver; (Ms) muscle; (K) kidney; (Ts) testis; (E7) 7-day embryo; (E11) 11-day embryo; (E15) 15-day embryo; (E17) 17-day embryo; (P) pancreas; (SI) small intestine; (LI) large intestine; (N) no template control; (−) plasmid containing Isoforms 1/2 is used as template for the amplification; and Ma, marker ϕX174/HaeIII.

Techniques Used: Expressing, Polymerase Chain Reaction, Amplification, Plasmid Preparation, Mass Spectrometry, Marker

Related Articles

Amplification:

Article Title: Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1 †
Article Snippet: .. After heat inactivation at 80°C for 20 min, 1 μl of the reaction mixture was amplified by PCR for 35 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C for 30 s, with an initial denaturation at 96°C for 2 min and a final extension at 72°C for 5 min. PCR mixtures contained 200 μM concentrations of each dNTP, 2 mM MgCl2 , 50 mM KCl, 0.5 U of Ex- Taq (TaKaRa), and 0.5 μM concentrations of each primer (see Table S1 in the supplemental material). .. Purified SART1 complex was overlaid onto 3.3 ml of 10 to 40% glycerol gradient in the HG500 buffer.

Article Title: Association between polymorphisms in the GRIN1 gene 5′ regulatory region and schizophrenia in a northern Han Chinese population and haplotype effects on protein expression in vitro
Article Snippet: .. Genomic DNA (1 μL, about 30 ng) was amplified under the following reaction contents: 1 μL (5 pmol) each of sense and antisense primers, 2 μL (3 nmol) of dNTP mix, 0.2 μL (about 0.5 U) of PrimeSTAR® HS DNA polymerase (Takara, Dalian, China) and 10 μL 2 × Prime STAR HS GC buffer. ..

DNA Synthesis:

Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

Marker:

Article Title: Small Molecular Contaminant and Microorganism Can Be Simultaneously Detected Based on Nanobody-Phage: Using Carcinogen Aflatoxin and Its Main Fungal Aspergillus Section Flavi spp. in Stored Maize for Demonstration
Article Snippet: .. DNA polymerase (iTaq), Mg2+ , dNTPs, 6× loading buffer, and DNA marker were bought from Takara Bio (Beijing, China). .. The anti-aflatoxins monoclonal antibody 1C11 (mAb 1C11) and V2–5 phage displaying nanobody specific for 1C11 were produced by our team ( ; ).

Activity Assay:

Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease
Article Snippet: .. Resumption of DNA Synthesis by DNA Polymerase after the Removal of CTNAs The hybridized oligonucleotides (400 fmol) were first treated with ERCC1-XPF (230 fmol) in 10 µL of 50 mM Tris-HCl buffer (pH 8.0), containing 2 mM MgCl2 , 0.5 mM DTT and 0.1 mg·mL−1 BSA, at 25 °C for 16 h. To the reaction mixture was added 5 µL of 30 mM Tris-HCl buffer (pH 7.9), containing 150 mM NaCl, 30 mM MgCl2 , 30 mM DTT, 300 µM dNTPs and the Klenow fragment of Escherichia coli DNA polymerase I, lacking the 3′–5′ exonuclease activity (KF− , 0.1 unit, Takara Bio). ..

Polymerase Chain Reaction:

Article Title: Essential Domains for Ribonucleoprotein Complex Formation Required for Retrotransposition of Telomere-Specific Non-Long Terminal Repeat Retrotransposon SART1 †
Article Snippet: .. After heat inactivation at 80°C for 20 min, 1 μl of the reaction mixture was amplified by PCR for 35 cycles of 98°C for 20 s, 60°C for 30 s, and 72°C for 30 s, with an initial denaturation at 96°C for 2 min and a final extension at 72°C for 5 min. PCR mixtures contained 200 μM concentrations of each dNTP, 2 mM MgCl2 , 50 mM KCl, 0.5 U of Ex- Taq (TaKaRa), and 0.5 μM concentrations of each primer (see Table S1 in the supplemental material). .. Purified SART1 complex was overlaid onto 3.3 ml of 10 to 40% glycerol gradient in the HG500 buffer.

Article Title: Functional variations of the TLR4 gene in association with chronic obstructive pulmonary disease and pulmonary tuberculosis
Article Snippet: .. PCR was performed to amplify the fragments of Wt, Mut1 and Mut2 in 0.5 ml tubes that included 0.5 μl of PrimeSTAR HS DNA polymerase (Takara), 1 μl of 10 μM forward and reverse primers, 4 μl of 2.5 mM each dNTP Mix (Takara), 10 ng of genomic DNA and 10 μl of 5 × PS Buffer (Takara). ..

Plasmid Preparation:

Article Title: A transcription and translation-coupled DNA replication system using rolling-circle replication
Article Snippet: .. Assay of the TTcDR reaction The optimized composition of the TTcDR system was as follows: template plasmid DNA (1 ng/μl), dNTPs (0.3 mM each, Takara), [32 -P] dCTP (3.3 μM, PerkinElmer), magnesium acetate (7.9 mM, Wako), potassium glutamate (70 mM, Wako), spermidine (0.375 mM, Nakarai), dithiothreitol (6 mM, Nakarai), ATP (0.375 mM, GE Healthcare), GTP (0.25 mM, GE Healthcare), CTP (0.125 mM, GE Healthcare), UTP (0.125 mM, GE Healthcare), creatine phosphate (25 mM, Nakarai), E. coli tRNA mixture (0.518 μg/μl, Roche), 10-formyl-5,6,7,8-tetrahydrofolic acid (10 ng/μl), Cys (0.3 mM, Wako), Tyr (0.3 mM, Wako), the other 18 amino acids except for Cys and Tyr (0.36 mM, Wako), 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (100 mM, pH 7.6, Sigma), ribosomes (1 μM), IF1 (25 μM), IF2 (1 μM), IF3 (4.9 μM), EF-G (1.1 μM), EF-Tu (80 μM), EF-Ts (3.3 μM), RF1 (0.05 μM), RF2 (0.05 μM), RF3 (0.17 μM), RRF (3.9 μM), AlaRS (730 nM), ArgRS (30 nM), AsnRS (420 nM), AspRS (120 nM), CysRS (20 nM), GlnRS (60 nM), GluRS (230 nM), GlyRS (90 nM), HisRS (90 nM), IleRS (370 nM), LeuRS (40 nM), LysRS (120 nM), MetRS (110 nM), PheRS (130 nM), ProRS (170 nM), SerRS (80 nM), ThrRS (80 nM), TrpRS (30 nM), TyrRS (150 nM), ValRS (20 nM), MTF (590 nM), creatine kinase (0.25 μM), myokinase (1.4 μM), nucleoside-diphosphate kinase (20 nM), pyrophosphatase (40 nM), yeast inorganic pyrophosphatase (0.2 mU/μl, New England BioLabs (NEB)), ribonuclease inhibitor (0.1 U/μl; Promega), and T7 RNA polymerase (0.42 U/μl; Takara). .. Most of the proteins described above were purified according to a previously described method except for yeast inorganic pyrophosphatase, ribonuclease inhibitor, and T7 RNA polymerase.

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    TaKaRa qrt pcr mixture
    Overexpression of PpGLK1 in u/u tomato. (A) <t>qRT-PCR</t> and western blot analysis confirming PpGLK1 overexpression in u/u tomato. (B) Phenotype of PpGLK1 -overexpressing u/u tomato. (C) Chlorophyll contents in u/u tomato and PpGLK1 -overexpressing u/u tomato. (D) Chloroplast development in fruit observed by TEM in PpGLK1 -overexpressing u/u tomato fruit and u/u tomato fruit. Data is presented as the means ± SD, n = 3. Different letters above bars denote statistical significance according to one-way ANOVA and Duncan's test ( P
    Qrt Pcr Mixture, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 28 article reviews
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    qrt pcr mixture - by Bioz Stars, 2020-09
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    Overexpression of PpGLK1 in u/u tomato. (A) qRT-PCR and western blot analysis confirming PpGLK1 overexpression in u/u tomato. (B) Phenotype of PpGLK1 -overexpressing u/u tomato. (C) Chlorophyll contents in u/u tomato and PpGLK1 -overexpressing u/u tomato. (D) Chloroplast development in fruit observed by TEM in PpGLK1 -overexpressing u/u tomato fruit and u/u tomato fruit. Data is presented as the means ± SD, n = 3. Different letters above bars denote statistical significance according to one-way ANOVA and Duncan's test ( P

    Journal: Frontiers in Plant Science

    Article Title: Transcriptomic and Functional Analyses Reveal That PpGLK1 Regulates Chloroplast Development in Peach (Prunus persica)

    doi: 10.3389/fpls.2018.00034

    Figure Lengend Snippet: Overexpression of PpGLK1 in u/u tomato. (A) qRT-PCR and western blot analysis confirming PpGLK1 overexpression in u/u tomato. (B) Phenotype of PpGLK1 -overexpressing u/u tomato. (C) Chlorophyll contents in u/u tomato and PpGLK1 -overexpressing u/u tomato. (D) Chloroplast development in fruit observed by TEM in PpGLK1 -overexpressing u/u tomato fruit and u/u tomato fruit. Data is presented as the means ± SD, n = 3. Different letters above bars denote statistical significance according to one-way ANOVA and Duncan's test ( P

    Article Snippet: The qRT-PCR mixture consisted of 10 μL SYBR Premix Ex Taq (TaKaRa Biotechnology, Dalian, China), 1 μL cDNA, and 1 μL each primer pair.

    Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Transmission Electron Microscopy

    qRT-PCR for detecting the transcription levels of genes related to the glutathione-based antioxidative system, including PpGPX1 , PpGLR1 , PpGSH1 , PpYAP1 , and PpPMP20 , in the Pichia pastoris transformants pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 when exposed to 50 mM H 2 O 2 . (A to E) Time course detection of the transcription level of PpGPX1 (A), PpGLR1 (B), PpGSH1 (C), PpYAP1 (D), and PpPMP20 (E) in the Pichia pastoris transformants pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 when exposed to 50 mM H 2 O 2 . To evaluate the change of gene transcription over time, the transcription levels of various genes at 0 h were set as 1-fold. (F) Comparison of the transcription levels of PpGPX1 , PpPMP20 , PpGLR1 , PpGSH1 , and PpYAP1 between pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 exposed to 50 mM H 2 O 2 for 12 h. The transcription levels of various genes in pPIC3.5K/GS115 were set as 1-fold. PpACT1 is the housekeeping gene that encodes the actin of Pichia pastoris. PpGPX1 encodes glutathione peroxidase. PpPMP20 encodes peroxisome glutathione peroxidase. PpGLR1 encodes glutathione reductase. PpGSH1 encodes γ-glutamylcysteine synthetase. PpYAP1 encodes the PpYAP1 transcription factor. Results are means ± standard deviations ( n = 3).

    Journal: Applied and Environmental Microbiology

    Article Title: Expression of the Laccase Gene from a White Rot Fungus in Pichia pastoris Can Enhance the Resistance of This Yeast to H2O2-Mediated Oxidative Stress by Stimulating the Glutathione-Based Antioxidative System

    doi: 10.1128/AEM.00218-12

    Figure Lengend Snippet: qRT-PCR for detecting the transcription levels of genes related to the glutathione-based antioxidative system, including PpGPX1 , PpGLR1 , PpGSH1 , PpYAP1 , and PpPMP20 , in the Pichia pastoris transformants pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 when exposed to 50 mM H 2 O 2 . (A to E) Time course detection of the transcription level of PpGPX1 (A), PpGLR1 (B), PpGSH1 (C), PpYAP1 (D), and PpPMP20 (E) in the Pichia pastoris transformants pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 when exposed to 50 mM H 2 O 2 . To evaluate the change of gene transcription over time, the transcription levels of various genes at 0 h were set as 1-fold. (F) Comparison of the transcription levels of PpGPX1 , PpPMP20 , PpGLR1 , PpGSH1 , and PpYAP1 between pPIC3.5K-lac5930-1/GS115 and pPIC3.5K/GS115 exposed to 50 mM H 2 O 2 for 12 h. The transcription levels of various genes in pPIC3.5K/GS115 were set as 1-fold. PpACT1 is the housekeeping gene that encodes the actin of Pichia pastoris. PpGPX1 encodes glutathione peroxidase. PpPMP20 encodes peroxisome glutathione peroxidase. PpGLR1 encodes glutathione reductase. PpGSH1 encodes γ-glutamylcysteine synthetase. PpYAP1 encodes the PpYAP1 transcription factor. Results are means ± standard deviations ( n = 3).

    Article Snippet: The qRT-PCR mixture (25 μl) contained 2.0 μl of cDNA and 0.4 μM each gene-specific primer as well as 1× SYBR Premix Ex Taq II (TaKaRa).

    Techniques: Quantitative RT-PCR

    2.3. Sequences of immune related genes and primer design for qRT-PCR

    Journal: Results in Immunology

    Article Title: Antimicrobial peptide gene induction, involvement of Toll and IMD pathways and defense against bacteria in the red flour beetle, Tribolium castaneum

    doi: 10.1016/j.rinim.2012.03.002

    Figure Lengend Snippet: 2.3. Sequences of immune related genes and primer design for qRT-PCR

    Article Snippet: Each qRT-PCR mixture (12.5 μl) contained 0.5 μl of first strand cDNA, and the real-time detection and analyses were done based on SYBR green dye chemistry using a SYBR Premix Ex Taq Perfect Real Time Kit (TAKARA) and a Thermal Cycler Dice Real Time System (model TP800, TAKARA).

    Techniques: Quantitative RT-PCR

    Knockdown of MyD88 and IMD at the mRNA level by dsRNA injection. Day 1 pupae were injected with 100 ng of either MyD88 or IMD dsRNA, and 72 h later the mRNA amounts of the targets were determined by qRT-PCR. Pupae injected with malE dsRNA

    Journal: Results in Immunology

    Article Title: Antimicrobial peptide gene induction, involvement of Toll and IMD pathways and defense against bacteria in the red flour beetle, Tribolium castaneum

    doi: 10.1016/j.rinim.2012.03.002

    Figure Lengend Snippet: Knockdown of MyD88 and IMD at the mRNA level by dsRNA injection. Day 1 pupae were injected with 100 ng of either MyD88 or IMD dsRNA, and 72 h later the mRNA amounts of the targets were determined by qRT-PCR. Pupae injected with malE dsRNA

    Article Snippet: Each qRT-PCR mixture (12.5 μl) contained 0.5 μl of first strand cDNA, and the real-time detection and analyses were done based on SYBR green dye chemistry using a SYBR Premix Ex Taq Perfect Real Time Kit (TAKARA) and a Thermal Cycler Dice Real Time System (model TP800, TAKARA).

    Techniques: Radial Immuno Diffusion, Injection, Quantitative RT-PCR

    Overexpression of PpGLK1 in u/u tomato. (A) qRT-PCR and western blot analysis confirming PpGLK1 overexpression in u/u tomato. (B) Phenotype of PpGLK1 -overexpressing u/u tomato. (C) Chlorophyll contents in u/u tomato and PpGLK1 -overexpressing u/u tomato. (D) Chloroplast development in fruit observed by TEM in PpGLK1 -overexpressing u/u tomato fruit and u/u tomato fruit. Data is presented as the means ± SD, n = 3. Different letters above bars denote statistical significance according to one-way ANOVA and Duncan's test ( P

    Journal: Frontiers in Plant Science

    Article Title: Transcriptomic and Functional Analyses Reveal That PpGLK1 Regulates Chloroplast Development in Peach (Prunus persica)

    doi: 10.3389/fpls.2018.00034

    Figure Lengend Snippet: Overexpression of PpGLK1 in u/u tomato. (A) qRT-PCR and western blot analysis confirming PpGLK1 overexpression in u/u tomato. (B) Phenotype of PpGLK1 -overexpressing u/u tomato. (C) Chlorophyll contents in u/u tomato and PpGLK1 -overexpressing u/u tomato. (D) Chloroplast development in fruit observed by TEM in PpGLK1 -overexpressing u/u tomato fruit and u/u tomato fruit. Data is presented as the means ± SD, n = 3. Different letters above bars denote statistical significance according to one-way ANOVA and Duncan's test ( P

    Article Snippet: The qRT-PCR mixture consisted of 10 μL SYBR Premix Ex Taq (TaKaRa Biotechnology, Dalian, China), 1 μL cDNA, and 1 μL each primer pair.

    Techniques: Over Expression, Quantitative RT-PCR, Western Blot, Transmission Electron Microscopy