pcr mix  (Qiagen)

 
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    QIAGEN PCR Cloning Kit
    Description:
    For direct cloning of PCR products generated by Taq DNA polymerases Kit contents Qiagen PCR Cloning Kit 10 rxns For Direct Cloning of PCR Products Generated by Taq DNA Polymerases pDrive Cloning Vector Just 40 min from PCR Product to Plated Cells Ready to use Ligation Master Mix High specificity UA Hybridization for Efficient Cloning Includes 2x Ligation Master Mix 50L pDrive Cloning Vector 0 5g Distilled Water 1 7mL Benefits Just 40 minutes from PCR product to plated cells Ready to use Ligation Master Mix High specificity UA hybridization for efficient cloning Immediate plating of transformed competent cells
    Catalog Number:
    231122
    Price:
    110
    Category:
    QIAGEN PCR Cloning Kit
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    Structured Review

    Qiagen pcr mix
    QIAGEN PCR Cloning Kit
    For direct cloning of PCR products generated by Taq DNA polymerases Kit contents Qiagen PCR Cloning Kit 10 rxns For Direct Cloning of PCR Products Generated by Taq DNA Polymerases pDrive Cloning Vector Just 40 min from PCR Product to Plated Cells Ready to use Ligation Master Mix High specificity UA Hybridization for Efficient Cloning Includes 2x Ligation Master Mix 50L pDrive Cloning Vector 0 5g Distilled Water 1 7mL Benefits Just 40 minutes from PCR product to plated cells Ready to use Ligation Master Mix High specificity UA hybridization for efficient cloning Immediate plating of transformed competent cells
    https://www.bioz.com/result/pcr mix/product/Qiagen
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    pcr mix - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Cellular retinol binding protein 1 could be a tumor suppressor gene in cervical cancer"

    Article Title: Cellular retinol binding protein 1 could be a tumor suppressor gene in cervical cancer

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Molecular events for CRBP1 gene in cervical epithelium samples. A: In order to know the gain of copy number of the CRBP1 gene, DNA of healthy cervix and CC samples, were subjected to real time PCR with specific Taqman probes. White bar (healthy cervix samples) represents the mean of the normal cervices (n = 26) without extra copies of CRBP1 ]. In X-axis represents cervical samples, Y-axis relative copies fold change of CRBP1 gene . B: CRBP1 expression was observed as positive immunostaining result on tissue microarray as mentioned in Methods section The DNAs used for gain of copy number (panel A) were also used for the methylation assay. Methylation result represents the methylation of the CRBP1 promoter. In this case, each healthy or CC sample, correspond to each column for CRBP1 expression and methylation status. Interestingly, in most of the cases, there was an association between the lack of expression of the CRBP1 gene and its methylation status.
    Figure Legend Snippet: Molecular events for CRBP1 gene in cervical epithelium samples. A: In order to know the gain of copy number of the CRBP1 gene, DNA of healthy cervix and CC samples, were subjected to real time PCR with specific Taqman probes. White bar (healthy cervix samples) represents the mean of the normal cervices (n = 26) without extra copies of CRBP1 ]. In X-axis represents cervical samples, Y-axis relative copies fold change of CRBP1 gene . B: CRBP1 expression was observed as positive immunostaining result on tissue microarray as mentioned in Methods section The DNAs used for gain of copy number (panel A) were also used for the methylation assay. Methylation result represents the methylation of the CRBP1 promoter. In this case, each healthy or CC sample, correspond to each column for CRBP1 expression and methylation status. Interestingly, in most of the cases, there was an association between the lack of expression of the CRBP1 gene and its methylation status.

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Immunostaining, Microarray, Methylation

    2) Product Images from "Aberrant cellular retinol binding protein 1 (CRBP1) gene expression and promoter methylation in prostate cancer"

    Article Title: Aberrant cellular retinol binding protein 1 (CRBP1) gene expression and promoter methylation in prostate cancer

    Journal: Journal of Clinical Pathology

    doi: 10.1136/jcp.2003.014555

    Example of methylation specific polymerase chain reaction for the CRBP1 promoter region in morphologically normal prostate tissue from radical prostatectomy specimens (N) and cystoprostatectomy specimens (CP). Lanes U and M correspond to unmethylated (109 bp) and methylated (99 bp) reactions, respectively. Sample 32N shows both unmethylated and methylated alleles, whereas samples 16N, 30N, and CP1 are completely unmethylated. Normal leucocyte DNA was used as a negative control for methylation (NL), DNA from normal leucocytes methylated with an excess of SssI bacterial methyltransferase was used as a positive control for methylation (IVD), and water was used as a negative polymerase chain reaction control (H 2 O). The HiLo marker is depicted on the right side.
    Figure Legend Snippet: Example of methylation specific polymerase chain reaction for the CRBP1 promoter region in morphologically normal prostate tissue from radical prostatectomy specimens (N) and cystoprostatectomy specimens (CP). Lanes U and M correspond to unmethylated (109 bp) and methylated (99 bp) reactions, respectively. Sample 32N shows both unmethylated and methylated alleles, whereas samples 16N, 30N, and CP1 are completely unmethylated. Normal leucocyte DNA was used as a negative control for methylation (NL), DNA from normal leucocytes methylated with an excess of SssI bacterial methyltransferase was used as a positive control for methylation (IVD), and water was used as a negative polymerase chain reaction control (H 2 O). The HiLo marker is depicted on the right side.

    Techniques Used: Methylation, Polymerase Chain Reaction, Negative Control, Positive Control, Marker

    Illustrative example of methylation specific polymerase chain reaction for the CRBP1 promoter region in high grade prostatic intraepithelial neoplasia (PIN) and adenocarcinoma (T). Lanes U and M correspond to unmethylated (109 bp) and methylated (99 bp) reactions, respectively. In patient 16, both lesions are methylated, whereas in patient 5 both are unmethylated. In patient 30, methylated alleles were only detected in the adenocarcinoma. In each case, normal leucocyte DNA was used as a negative control for methylation (NL), DNA from normal leucocytes methylated with an excess of SssI bacterial methyltransferase was used as a positive control for methylation (IVD), and water was used as a negative polymerase chain reaction control (H 2 O). The HiLo marker is depicted on the right side.
    Figure Legend Snippet: Illustrative example of methylation specific polymerase chain reaction for the CRBP1 promoter region in high grade prostatic intraepithelial neoplasia (PIN) and adenocarcinoma (T). Lanes U and M correspond to unmethylated (109 bp) and methylated (99 bp) reactions, respectively. In patient 16, both lesions are methylated, whereas in patient 5 both are unmethylated. In patient 30, methylated alleles were only detected in the adenocarcinoma. In each case, normal leucocyte DNA was used as a negative control for methylation (NL), DNA from normal leucocytes methylated with an excess of SssI bacterial methyltransferase was used as a positive control for methylation (IVD), and water was used as a negative polymerase chain reaction control (H 2 O). The HiLo marker is depicted on the right side.

    Techniques Used: Methylation, Polymerase Chain Reaction, Negative Control, Positive Control, Marker

    3) Product Images from "Advances in multiplex PCR: balancing primer efficiencies and improving detection success"

    Article Title: Advances in multiplex PCR: balancing primer efficiencies and improving detection success

    Journal: Methods in Ecology and Evolution

    doi: 10.1111/j.2041-210X.2012.00215.x

    Multiplex PCR conducted with standardised numbers of DNA templates and separated with QIAxcel (Qiagen) where an internal marker (15 and 3000 bp) is run with each sample. Description of Lanes: Pn, Pardosa nigra ; Nr, Nebria rufescens ; Oc, Oreonebria castanea ; Mg, Mitopus glacialis ; Nj, Nebria jockischii ; Ng, Nebria germari ; Col, Collembola; each with 10 000 double-stranded copies as template (tc); M1–M4 standardised DNA mixes. M1, 2100 tc per target; M2, 1000 tc per target; M3, 200 tc per target; M4, 100 tc per target; E, electropherogram of Lane M3. Note: when a single target was present at high concentrations, signal strength was not balanced (e.g. Oc and Ng resulted in stronger signals); however, this did not occur at lower concentrations.
    Figure Legend Snippet: Multiplex PCR conducted with standardised numbers of DNA templates and separated with QIAxcel (Qiagen) where an internal marker (15 and 3000 bp) is run with each sample. Description of Lanes: Pn, Pardosa nigra ; Nr, Nebria rufescens ; Oc, Oreonebria castanea ; Mg, Mitopus glacialis ; Nj, Nebria jockischii ; Ng, Nebria germari ; Col, Collembola; each with 10 000 double-stranded copies as template (tc); M1–M4 standardised DNA mixes. M1, 2100 tc per target; M2, 1000 tc per target; M3, 200 tc per target; M4, 100 tc per target; E, electropherogram of Lane M3. Note: when a single target was present at high concentrations, signal strength was not balanced (e.g. Oc and Ng resulted in stronger signals); however, this did not occur at lower concentrations.

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Marker

    4) Product Images from "Human herpesvirus–encoded kinase induces B cell lymphomas in vivo"

    Article Title: Human herpesvirus–encoded kinase induces B cell lymphomas in vivo

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI97053

    Lymphomas from individual aged vPK mice are monoclonal or polyclonal. D–JH segments of IgH in genomic DNA were PCR amplified, electrophoresed, and stained with ethidium bromide. D–JH segments of IgH in normal WT spleens (WT Sp) and vPK lymphomas. Node, mouse 3; liver, mice 4, 7, and 13; spleen, mice 6, 8–12, and 14. Asterisks indicate the preferential amplification of 1 D–JH segment. A total of 20 lymphoma tissues each from a different aged vPK mouse were evaluated. Polyclonal, n = 12; monoclonal, n = 8.
    Figure Legend Snippet: Lymphomas from individual aged vPK mice are monoclonal or polyclonal. D–JH segments of IgH in genomic DNA were PCR amplified, electrophoresed, and stained with ethidium bromide. D–JH segments of IgH in normal WT spleens (WT Sp) and vPK lymphomas. Node, mouse 3; liver, mice 4, 7, and 13; spleen, mice 6, 8–12, and 14. Asterisks indicate the preferential amplification of 1 D–JH segment. A total of 20 lymphoma tissues each from a different aged vPK mouse were evaluated. Polyclonal, n = 12; monoclonal, n = 8.

    Techniques Used: Mouse Assay, Polymerase Chain Reaction, Amplification, Staining

    Generation of vPK transgenic mice. FLAG-tagged vPK was cloned, using HinDIII-HF and BamHI-HF, into a plasmid containing a Ub- and hGH-stabilization element. The linearized transgene fragment was microinjected into the embryos of C57BL/6 mice and implanted into pseudopregnant female mice. Each vPK line was developed from breeding a vPK founder to a C57BL/6 mouse. ( A ) Schematic of the linearized construct used to generate the vPK transgenic mice, as well as the cut sites and probe for the Southern blot in D . ( B ) DNA from the tails of 2 WT (wA and wB) and 2 vPK transgenic mice (vA and vB) for lines 1 and 2 was isolated and evaluated by PCR for the vPK transgene. ( C ) Expression of vPK protein in lysates from spleens of the mice in B as determined by SDS-PAGE and Western blot. Lysate from HEK-293 cells that transiently express vPK was used as a positive control for vPK expression. ( D ) Southern blot of WT, vPK1, and vPK2. DNA was isolated from the spleens of WT and vPK lines 1 and 2. D, double digest with HinDIII-HF and BamHI-HF; S, single digestion with AFlII; p-vPK, plasmid Ub.vPK.hGH.
    Figure Legend Snippet: Generation of vPK transgenic mice. FLAG-tagged vPK was cloned, using HinDIII-HF and BamHI-HF, into a plasmid containing a Ub- and hGH-stabilization element. The linearized transgene fragment was microinjected into the embryos of C57BL/6 mice and implanted into pseudopregnant female mice. Each vPK line was developed from breeding a vPK founder to a C57BL/6 mouse. ( A ) Schematic of the linearized construct used to generate the vPK transgenic mice, as well as the cut sites and probe for the Southern blot in D . ( B ) DNA from the tails of 2 WT (wA and wB) and 2 vPK transgenic mice (vA and vB) for lines 1 and 2 was isolated and evaluated by PCR for the vPK transgene. ( C ) Expression of vPK protein in lysates from spleens of the mice in B as determined by SDS-PAGE and Western blot. Lysate from HEK-293 cells that transiently express vPK was used as a positive control for vPK expression. ( D ) Southern blot of WT, vPK1, and vPK2. DNA was isolated from the spleens of WT and vPK lines 1 and 2. D, double digest with HinDIII-HF and BamHI-HF; S, single digestion with AFlII; p-vPK, plasmid Ub.vPK.hGH.

    Techniques Used: Transgenic Assay, Mouse Assay, Clone Assay, Plasmid Preparation, Construct, Southern Blot, Western Blot, Isolation, Polymerase Chain Reaction, Expressing, SDS Page, Positive Control

    5) Product Images from "Molecular Detection of Human Metapneumovirus and Human Bocavirus on Oropharyngeal Swabs Collected from Young Children with Acute Respiratory Tract Infections from Rural and Peri-Urban Communities in South India"

    Article Title: Molecular Detection of Human Metapneumovirus and Human Bocavirus on Oropharyngeal Swabs Collected from Young Children with Acute Respiratory Tract Infections from Rural and Peri-Urban Communities in South India

    Journal: Molecular Diagnosis & Therapy

    doi: 10.1007/s40291-013-0030-y

    Gel picture showing amplification of a human metapneumovirus (hMPV) semi-nested polymerase chain reaction (PCR) and b human bocavirus (hBoV) one-step PCR in patient samples. a Lanes 1 and 4 patient samples showing specific amplification for hMPV at 365 bp; lane 16 positive control; lane 17 molecular weight marker (100 bp ladder). b Lane 1 patient sample showing specific amplification for HBoV; lane 10 positive control; lane 11 molecular weight marker (100 bp ladder)
    Figure Legend Snippet: Gel picture showing amplification of a human metapneumovirus (hMPV) semi-nested polymerase chain reaction (PCR) and b human bocavirus (hBoV) one-step PCR in patient samples. a Lanes 1 and 4 patient samples showing specific amplification for hMPV at 365 bp; lane 16 positive control; lane 17 molecular weight marker (100 bp ladder). b Lane 1 patient sample showing specific amplification for HBoV; lane 10 positive control; lane 11 molecular weight marker (100 bp ladder)

    Techniques Used: Amplification, Nested PCR, Polymerase Chain Reaction, Positive Control, Molecular Weight, Marker

    6) Product Images from "De novo analysis of transcriptome reveals genes associated with leaf abscission in sugarcane (Saccharum officinarum L.)"

    Article Title: De novo analysis of transcriptome reveals genes associated with leaf abscission in sugarcane (Saccharum officinarum L.)

    Journal: BMC Genomics

    doi: 10.1186/s12864-016-2552-2

    qRT-PCR validation for candidate transcripts
    Figure Legend Snippet: qRT-PCR validation for candidate transcripts

    Techniques Used: Quantitative RT-PCR

    7) Product Images from "A Dual-Modality Herpes Simplex Virus 2 Vaccine for Preventing Genital Herpes by Using Glycoprotein C and D Subunit Antigens To Induce Potent Antibody Responses and Adenovirus Vectors Containing Capsid and Tegument Proteins as T Cell Immunogens"

    Article Title: A Dual-Modality Herpes Simplex Virus 2 Vaccine for Preventing Genital Herpes by Using Glycoprotein C and D Subunit Antigens To Induce Potent Antibody Responses and Adenovirus Vectors Containing Capsid and Tegument Proteins as T Cell Immunogens

    Journal: Journal of Virology

    doi: 10.1128/JVI.01089-15

    HSV-2 DNA copies and detection of LAT in DRG. DRG were isolated at 81 days postchallenge and analyzed for HSV-2 DNA copies. (A) HSV-2 DNA log 10 copy numbers in DRG. (B and C) LAT and GAPDH transcripts in DRG were plotted as real-time PCR C T values. The gray boxes indicate that there was no transcript amplification by 45 PCR cycles. The negative LAT samples were assigned a C T value of 46 ( > 45 cycles) for calculations of mean ± SEM values. C T values rather than copy numbers were used to express results because purified RNA was not available to develop standard curves.
    Figure Legend Snippet: HSV-2 DNA copies and detection of LAT in DRG. DRG were isolated at 81 days postchallenge and analyzed for HSV-2 DNA copies. (A) HSV-2 DNA log 10 copy numbers in DRG. (B and C) LAT and GAPDH transcripts in DRG were plotted as real-time PCR C T values. The gray boxes indicate that there was no transcript amplification by 45 PCR cycles. The negative LAT samples were assigned a C T value of 46 ( > 45 cycles) for calculations of mean ± SEM values. C T values rather than copy numbers were used to express results because purified RNA was not available to develop standard curves.

    Techniques Used: Isolation, Real-time Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Purification

    Related Articles

    DNA Extraction:

    Article Title: The Circular RNA Interacts with STAT3, Increasing Its Nuclear Translocation and Wound Repair by Modulating Dnmt3a and miR-17 Function
    Article Snippet: .. RNA and DNA extraction kits and RNA RT and PCR kits were obtained from QIAGEN. .. Immunoblotting was performed using the ECL western blot detection kit (Amersham Biosciences).

    Clone Assay:

    Article Title: Transcription-Associated R-Loop Formation across the Human FMR1 CGG-Repeat Region
    Article Snippet: .. PCR-amplified DNA was sub-cloned using the Qiagen PCR Cloning Kit. .. Chemically competent E. coli Top10 cells (Life Technologies) were transformed by heat-shock with ligated plasmid, and were grown overnight at 37°C on LB agar plates with 100 mg/ml ampicillin selection.

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools
    Article Snippet: .. Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen). .. Sequencing was performed by GENEWIZ and BaseClear, and the resulting sequence data were analyzed using Sequencher software (Gene Codes).

    Article Title: A Quantitative PCR Protocol for Detection of Oxyspirura petrowi in Northern Bobwhites (Colinus virginianus)
    Article Snippet: .. Standard Curve and Assay Optimization To create a standard curve to determine qPCR efficiency we cloned a 244 base pair fragment using Qiagen PCR Cloning plus kit (Qiagen Inc., Germantown, MD). ..

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    Article Title: Expression of microRNA-146 in osteoarthritis cartilage
    Article Snippet: .. For in situ hybridization, primary miR-146a fragments were derived from PCR products, cloned using the Qiagen PCR cloning kit into the pDrive vector (Qiagen, Chatsworth, CA), and sequenced. ..

    Article Title: Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing
    Article Snippet: .. Fungal rDNA amplicons were cloned into the pDRIVE vector using a PCR cloning kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Ligated plasmids were then transformed into TOP10 chemically competent Escherichia coli cells (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions.

    Amplification:

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    Purification:

    Article Title: Canonical and Atypical E2Fs Regulate the Mammalian Endocycle
    Article Snippet: .. Recovered DNA was purified using Qiagen PCR extraction kit. .. 5% input and 2µl of eluted DNA was used for quantitative Real-Time PCR (RT-PCR) with gene-specific primers and SYBR Green (BioRad 170–8880).

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    Real-time Polymerase Chain Reaction:

    Article Title: A Quantitative PCR Protocol for Detection of Oxyspirura petrowi in Northern Bobwhites (Colinus virginianus)
    Article Snippet: .. Standard Curve and Assay Optimization To create a standard curve to determine qPCR efficiency we cloned a 244 base pair fragment using Qiagen PCR Cloning plus kit (Qiagen Inc., Germantown, MD). ..

    Polymerase Chain Reaction:

    Article Title: Transcription-Associated R-Loop Formation across the Human FMR1 CGG-Repeat Region
    Article Snippet: .. PCR-amplified DNA was sub-cloned using the Qiagen PCR Cloning Kit. .. Chemically competent E. coli Top10 cells (Life Technologies) were transformed by heat-shock with ligated plasmid, and were grown overnight at 37°C on LB agar plates with 100 mg/ml ampicillin selection.

    Article Title: Telomere Length Homeostasis Responds to Changes in Intracellular dNTP Pools
    Article Snippet: .. Telomere VI-R PCR products were cloned using a PCR Cloning Kit (Qiagen). .. Sequencing was performed by GENEWIZ and BaseClear, and the resulting sequence data were analyzed using Sequencher software (Gene Codes).

    Article Title: Canonical and Atypical E2Fs Regulate the Mammalian Endocycle
    Article Snippet: .. Recovered DNA was purified using Qiagen PCR extraction kit. .. 5% input and 2µl of eluted DNA was used for quantitative Real-Time PCR (RT-PCR) with gene-specific primers and SYBR Green (BioRad 170–8880).

    Article Title: A Quantitative PCR Protocol for Detection of Oxyspirura petrowi in Northern Bobwhites (Colinus virginianus)
    Article Snippet: .. Standard Curve and Assay Optimization To create a standard curve to determine qPCR efficiency we cloned a 244 base pair fragment using Qiagen PCR Cloning plus kit (Qiagen Inc., Germantown, MD). ..

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    Article Title: The Circular RNA Interacts with STAT3, Increasing Its Nuclear Translocation and Wound Repair by Modulating Dnmt3a and miR-17 Function
    Article Snippet: .. RNA and DNA extraction kits and RNA RT and PCR kits were obtained from QIAGEN. .. Immunoblotting was performed using the ECL western blot detection kit (Amersham Biosciences).

    Article Title: Expression of microRNA-146 in osteoarthritis cartilage
    Article Snippet: .. For in situ hybridization, primary miR-146a fragments were derived from PCR products, cloned using the Qiagen PCR cloning kit into the pDrive vector (Qiagen, Chatsworth, CA), and sequenced. ..

    Article Title: Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing
    Article Snippet: .. Fungal rDNA amplicons were cloned into the pDRIVE vector using a PCR cloning kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Ligated plasmids were then transformed into TOP10 chemically competent Escherichia coli cells (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    Article Title: Expression of microRNA-146 in osteoarthritis cartilage
    Article Snippet: .. For in situ hybridization, primary miR-146a fragments were derived from PCR products, cloned using the Qiagen PCR cloning kit into the pDrive vector (Qiagen, Chatsworth, CA), and sequenced. ..

    Article Title: Comparison of DNA extraction methodologies used for assessing fungal diversity via ITS sequencing
    Article Snippet: .. Fungal rDNA amplicons were cloned into the pDRIVE vector using a PCR cloning kit (Qiagen, Valencia, CA, USA) according to the manufacturer’s instructions. .. Ligated plasmids were then transformed into TOP10 chemically competent Escherichia coli cells (Invitrogen, Carlsbad, CA, USA) according to manufacturer’s instructions.

    Sequencing:

    Article Title: Recombination Drives Evolution of the Clostridium difficile 16S-23S rRNA Intergenic Spacer Region
    Article Snippet: .. Cloning and sequencing of 16S-23S rRNA ISR The purified amplification products were cloned into the pDrive vector (PCR Cloning Plus kit; Qiagen) according to the manufactureŕs instructions. .. Plasmids were isolated from transformed overnight colonies using QIAprep Spin Miniprep kit (Qiagen).

    In Situ Hybridization:

    Article Title: Expression of microRNA-146 in osteoarthritis cartilage
    Article Snippet: .. For in situ hybridization, primary miR-146a fragments were derived from PCR products, cloned using the Qiagen PCR cloning kit into the pDrive vector (Qiagen, Chatsworth, CA), and sequenced. ..

    Derivative Assay:

    Article Title: Expression of microRNA-146 in osteoarthritis cartilage
    Article Snippet: .. For in situ hybridization, primary miR-146a fragments were derived from PCR products, cloned using the Qiagen PCR cloning kit into the pDrive vector (Qiagen, Chatsworth, CA), and sequenced. ..

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    Qiagen multiplex pcr mix
    Multiplex <t>PCR</t> assay using three specific markers for the perilla subspecies in a single reaction. A mixture of three specific markers, PfLS , PfMyb - P1pro , and PfDFRpro , was used for PCR amplification. Lanes on 3% electrophoresis gel: M: 100 bp DNA ladder; 1–3: ‘Cham-Dlggae’, ‘Ip-Dlggae’, and ‘Bora-Dlggae’, respectively, which represent the three cultivar types of P . frutescens var. japonica ; 4: ‘Jureum-soyeop’, representing P . frutescens var. crispa ; 5: <t>‘Jasoyeop’,</t> representing P . frutescens var. acuta ; 6: ‘Chungsoyeop’, representing P . frutescens f. viridis .
    Multiplex Pcr Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr mix/product/Qiagen
    Average 90 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr mix - by Bioz Stars, 2020-09
    90/100 stars
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    94
    Qiagen pcr mixture
    Multiplex <t>PCR</t> assay for SCC mec typing. Lane 1: negative control; Lane 2: positive control; Lanes 3, 4, and 6: 433-bp PVL gene fragment; Lane 5: isolate negative for the PVL gene; Lane M: 100-bp <t>DNA</t> ladder
    Pcr Mixture, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr mixture/product/Qiagen
    Average 94 stars, based on 503 article reviews
    Price from $9.99 to $1999.99
    pcr mixture - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Multiplex PCR assay using three specific markers for the perilla subspecies in a single reaction. A mixture of three specific markers, PfLS , PfMyb - P1pro , and PfDFRpro , was used for PCR amplification. Lanes on 3% electrophoresis gel: M: 100 bp DNA ladder; 1–3: ‘Cham-Dlggae’, ‘Ip-Dlggae’, and ‘Bora-Dlggae’, respectively, which represent the three cultivar types of P . frutescens var. japonica ; 4: ‘Jureum-soyeop’, representing P . frutescens var. crispa ; 5: ‘Jasoyeop’, representing P . frutescens var. acuta ; 6: ‘Chungsoyeop’, representing P . frutescens f. viridis .

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Development of User-Friendly Method to Distinguish Subspecies of the Korean Medicinal Herb Perilla frutescens Using Multiplex-PCR

    doi: 10.3390/molecules22040665

    Figure Lengend Snippet: Multiplex PCR assay using three specific markers for the perilla subspecies in a single reaction. A mixture of three specific markers, PfLS , PfMyb - P1pro , and PfDFRpro , was used for PCR amplification. Lanes on 3% electrophoresis gel: M: 100 bp DNA ladder; 1–3: ‘Cham-Dlggae’, ‘Ip-Dlggae’, and ‘Bora-Dlggae’, respectively, which represent the three cultivar types of P . frutescens var. japonica ; 4: ‘Jureum-soyeop’, representing P . frutescens var. crispa ; 5: ‘Jasoyeop’, representing P . frutescens var. acuta ; 6: ‘Chungsoyeop’, representing P . frutescens f. viridis .

    Article Snippet: Genomic DNA samples isolated from commercial perilla cultivars and dried Jasoyeop products were amplified in a multiplex PCR mix containing 25 μL 2x Qiagen Multiplex PCR master mix, 5 μL 5x Q-solution, 20 ng template DNA, and 5 μL 10x primer mix (final primer concentration: 0.5 μM PfLS , 1 μM PfMyb -P1 , and 1 μM PfDFR ).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Amplification, Electrophoresis

    Multiplex PCR identification of 11 commercial dried leaves and twigs of Jasoyeop products. ( a ) Lanes on 3% electrophoresis gel: M: 100 bp DNA ladder; A–K: purchased commercial dried Jasoyeop products (see Table 3 for full details); ( b , c ) Sequence analysis of PCR products amplified using the PfMybpro and PfDFRpro marker primers, respectively, aligned using Clustal W2.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Development of User-Friendly Method to Distinguish Subspecies of the Korean Medicinal Herb Perilla frutescens Using Multiplex-PCR

    doi: 10.3390/molecules22040665

    Figure Lengend Snippet: Multiplex PCR identification of 11 commercial dried leaves and twigs of Jasoyeop products. ( a ) Lanes on 3% electrophoresis gel: M: 100 bp DNA ladder; A–K: purchased commercial dried Jasoyeop products (see Table 3 for full details); ( b , c ) Sequence analysis of PCR products amplified using the PfMybpro and PfDFRpro marker primers, respectively, aligned using Clustal W2.

    Article Snippet: Genomic DNA samples isolated from commercial perilla cultivars and dried Jasoyeop products were amplified in a multiplex PCR mix containing 25 μL 2x Qiagen Multiplex PCR master mix, 5 μL 5x Q-solution, 20 ng template DNA, and 5 μL 10x primer mix (final primer concentration: 0.5 μM PfLS , 1 μM PfMyb -P1 , and 1 μM PfDFR ).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Electrophoresis, Sequencing, Amplification, Marker

    Multiplex PCR assay for SCC mec typing. Lane 1: negative control; Lane 2: positive control; Lanes 3, 4, and 6: 433-bp PVL gene fragment; Lane 5: isolate negative for the PVL gene; Lane M: 100-bp DNA ladder

    Journal: Pakistan Journal of Medical Sciences

    Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals

    doi:

    Figure Lengend Snippet: Multiplex PCR assay for SCC mec typing. Lane 1: negative control; Lane 2: positive control; Lanes 3, 4, and 6: 433-bp PVL gene fragment; Lane 5: isolate negative for the PVL gene; Lane M: 100-bp DNA ladder

    Article Snippet: The 50-µL PCR mixture contained 8 µL of DNA template, 1 µL (100 pmol) of each primer, and a 25 µL of Taq PCR Master Mix polymerase containing 100 mM Tris-HCl, 500 mM KCl (pH 8.3), 1.5 mM MgCl2 , 200 µM of each deoxyribonucleoside triphosphate, and 0.025 U of Taq polymerase (Qiagen, USA).

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Negative Control, Positive Control

    PCR detection of the PVL gene among selected MRSA isolates. Lane 1: negative control; Lane 2: positive control; Lanes 3, 4, and 6: 433-bp PVL gene fragment; Lane 5: isolate negative for the PVL gene; Lane M: 100-bp DNA ladder

    Journal: Pakistan Journal of Medical Sciences

    Article Title: Molecular characterization of methicillin-resistant Staphylococcus aureus isolated from tertiary care hospitals

    doi:

    Figure Lengend Snippet: PCR detection of the PVL gene among selected MRSA isolates. Lane 1: negative control; Lane 2: positive control; Lanes 3, 4, and 6: 433-bp PVL gene fragment; Lane 5: isolate negative for the PVL gene; Lane M: 100-bp DNA ladder

    Article Snippet: The 50-µL PCR mixture contained 8 µL of DNA template, 1 µL (100 pmol) of each primer, and a 25 µL of Taq PCR Master Mix polymerase containing 100 mM Tris-HCl, 500 mM KCl (pH 8.3), 1.5 mM MgCl2 , 200 µM of each deoxyribonucleoside triphosphate, and 0.025 U of Taq polymerase (Qiagen, USA).

    Techniques: Polymerase Chain Reaction, Negative Control, Positive Control

    RT-PCR analysis of tonB1 and tbpB expression. (a) RT-PCR analysis of tonB1 expression using primers tonBGER1 and tonBGER4. Lanes contain products from RNA templates under different growth conditions as follows: 1, wild type, iron replete; 2, wild type, iron restricted; 3, tonB1 mutant, iron restricted; 4, tonB1 ::pLURO3, iron restricted; 5, tonB2 mutant, iron restricted. M, size marker. (b) RT-PCR analysis of tbpB expression in A. pleuropneumoniae grown under conditions of iron restriction, using primers tbpB1 and tbpB2. Lanes contain products from RNA or DNA templates as follows: 1, wild-type RNA; 2, tonB1 mutant RNA; 3, wild-type RNA treated with RNase A (negative control); 4, tonB1 mutant RNA treated with RNase A (negative control); 5, wild-type DNA (positive control).

    Journal: Infection and Immunity

    Article Title: Two TonB Systems in Actinobacillus pleuropneumoniae: Their Roles in Iron Acquisition and Virulence

    doi: 10.1128/IAI.72.2.701-708.2004

    Figure Lengend Snippet: RT-PCR analysis of tonB1 and tbpB expression. (a) RT-PCR analysis of tonB1 expression using primers tonBGER1 and tonBGER4. Lanes contain products from RNA templates under different growth conditions as follows: 1, wild type, iron replete; 2, wild type, iron restricted; 3, tonB1 mutant, iron restricted; 4, tonB1 ::pLURO3, iron restricted; 5, tonB2 mutant, iron restricted. M, size marker. (b) RT-PCR analysis of tbpB expression in A. pleuropneumoniae grown under conditions of iron restriction, using primers tbpB1 and tbpB2. Lanes contain products from RNA or DNA templates as follows: 1, wild-type RNA; 2, tonB1 mutant RNA; 3, wild-type RNA treated with RNase A (negative control); 4, tonB1 mutant RNA treated with RNase A (negative control); 5, wild-type DNA (positive control).

    Article Snippet: Negative control experiments to test for the presence of contaminating DNA were performed by incubating the RT-PCR mixture (prior to the addition of enzyme) with 4 μl of RNase A (100 mg/ml) for 15 min at 37°C or by using HotStart Taq (Qiagen) instead of the supplied enzyme mixture, thus eliminating the reverse transcriptase step.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Mutagenesis, Marker, Negative Control, Positive Control

    RT-PCR analysis. (a) Products of RT-PCR of wild-type A. pleuropneumoniae grown under iron-restricted conditions, with primers tonB11 ( exbB2 ) and tonB16 ( tonB2 ) and templates as follows. Lane 1, total RNA treated with RNase A (negative control); lane 2, total RNA; lane 3, chromosomal DNA (positive control). M, size marker. (b) RT-PCR analysis of tonB expression in wild-type A. pleuropneumoniae , with primers tonBGER1 and tonBGER4 for tonB1 (lanes 1 to 4) and primers tonB10 and tonB18 for tonB2 (lanes 5 to 8), to generate bands between 200 and 400 bp as indicated. Templates were as follows. Lanes 1 and 5, RNA treated with RNase A, extracted from bacteria grown under iron-restricted conditions (negative control); lanes 2 and 6, RNA from bacteria grown under iron-replete conditions; lanes 3 and 7, RNA from bacteria grown under iron-restricted conditions; lanes 4 and 8, whole cellular DNA (positive control).

    Journal: Infection and Immunity

    Article Title: Two TonB Systems in Actinobacillus pleuropneumoniae: Their Roles in Iron Acquisition and Virulence

    doi: 10.1128/IAI.72.2.701-708.2004

    Figure Lengend Snippet: RT-PCR analysis. (a) Products of RT-PCR of wild-type A. pleuropneumoniae grown under iron-restricted conditions, with primers tonB11 ( exbB2 ) and tonB16 ( tonB2 ) and templates as follows. Lane 1, total RNA treated with RNase A (negative control); lane 2, total RNA; lane 3, chromosomal DNA (positive control). M, size marker. (b) RT-PCR analysis of tonB expression in wild-type A. pleuropneumoniae , with primers tonBGER1 and tonBGER4 for tonB1 (lanes 1 to 4) and primers tonB10 and tonB18 for tonB2 (lanes 5 to 8), to generate bands between 200 and 400 bp as indicated. Templates were as follows. Lanes 1 and 5, RNA treated with RNase A, extracted from bacteria grown under iron-restricted conditions (negative control); lanes 2 and 6, RNA from bacteria grown under iron-replete conditions; lanes 3 and 7, RNA from bacteria grown under iron-restricted conditions; lanes 4 and 8, whole cellular DNA (positive control).

    Article Snippet: Negative control experiments to test for the presence of contaminating DNA were performed by incubating the RT-PCR mixture (prior to the addition of enzyme) with 4 μl of RNase A (100 mg/ml) for 15 min at 37°C or by using HotStart Taq (Qiagen) instead of the supplied enzyme mixture, thus eliminating the reverse transcriptase step.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Negative Control, Positive Control, Marker, Expressing

    RT-PCR, sequencing, and analysis of VP1 genes for 92 nonserotypeable HEV clinical isolates.

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Identification and Analysis of Nonserotypeable Human Enteroviruses ▿Molecular Identification and Analysis of Nonserotypeable Human Enteroviruses ▿ †

    doi: 10.1128/JCM.02384-09

    Figure Lengend Snippet: RT-PCR, sequencing, and analysis of VP1 genes for 92 nonserotypeable HEV clinical isolates.

    Article Snippet: For VP1 PCR, the PCR mixture contained the following: 2 μl template cDNA; 1 μl VP1-All-Sn (100 pmol μl−1 ); 0.5 μl each of VP1-A-Ab, VP1-B-Ab, and VP1-C-Ab (100 pmol μl−1 ); 2.4 μl dNTPs (2.5 mM each dNTP); 3 μl 10× PCR buffer; 4.2 μl MgCl2 (25 mM); 0.2 μl Qiagen HotStarTaq DNA polymerase (5 units μl−1 ); and water to 30 μl.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing