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TaKaRa pcr machine
Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr machine/product/TaKaRa
Average 93 stars, based on 10 article reviews
Price from $9.99 to $1999.99
pcr machine - by Bioz Stars, 2020-02
93/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Mechanistic Studies of Anti-Hyperpigmentary Compounds: Elucidating Their Inhibitory and Regulatory Actions
Article Snippet: PCR machine was the product of Takara Bio Inc. (Otsu, Shiga, Japan). .. The cAMP EIA kit was purchased from Cayman Chemical Company (Ann Arbor, MI, USA).

RNA Extraction:

Article Title: Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes
Article Snippet: Paragraph title: 4.5. RNA Extraction and cDNA Synthesis ... RNA (50 ng) was converted into cDNA using a PCR machine (TaKaRa Bio, Kusatsu, Shiga, Japan) under the following conditions, which include initial incubation at 70 °C for 5 min followed by primer annealing at 25 °C for 5 min, extension at 42 °C for 60 min, and inactivation at 70 °C for 15 min.

Amplification:

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: .. The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio). ..

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: .. The transcribed products were mixed with each primer set and Go Taq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio). ..

Article Title: Association between Genetic Polymorphism of Multidrug Resistance 1 Gene and Sasang Constitutions
Article Snippet: .. The PCR reaction was amplified using a PCR machine (TAKARA, Japan). ..

Agarose Gel Electrophoresis:

Article Title: Association between Genetic Polymorphism of Multidrug Resistance 1 Gene and Sasang Constitutions
Article Snippet: The PCR reaction was amplified using a PCR machine (TAKARA, Japan). .. Electrophoresis of the reactant in 2% agarose gel confirmed the presence of the amplicons obtained from the PCR.

Polymerase Chain Reaction:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. Next, the compounds were spun down and inserted into PCR machine with 5-min pre-denaturation program at 95°C, 35 denaturation cycles at 95°C in 30 s, 30-min annealing at 55°C, and 30-s extension at 72°C. .. The making of agarose gel First, 0.060 g agarose was added with 30 mL Tris-Boric acid-EDTA (TBE) buffer pH 8.3 and heated in a microwave until being dissolved.

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini). .. The female mice were induced with PMSG (Folligon) with a dosage of 5 IU and followed by 5 IU human chorionic gonadotropin (Chorulon) injection after 48 h. The female mice were mated with vasectomized male mice to induce ovulation.

Article Title: Mechanistic Studies of Anti-Hyperpigmentary Compounds: Elucidating Their Inhibitory and Regulatory Actions
Article Snippet: .. PCR machine was the product of Takara Bio Inc. (Otsu, Shiga, Japan). .. NanoDrop 2000c spectrophotometer for nucleic acid measurement was purchased from Thermo Scientific (Wilmington, DE, USA).

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: .. The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio). ..

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: .. The transcribed products were mixed with each primer set and Go Taq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio). ..

Article Title: Association between Genetic Polymorphism of Multidrug Resistance 1 Gene and Sasang Constitutions
Article Snippet: .. The PCR reaction was amplified using a PCR machine (TAKARA, Japan). ..

Article Title: Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes
Article Snippet: .. RNA (50 ng) was converted into cDNA using a PCR machine (TaKaRa Bio, Kusatsu, Shiga, Japan) under the following conditions, which include initial incubation at 70 °C for 5 min followed by primer annealing at 25 °C for 5 min, extension at 42 °C for 60 min, and inactivation at 70 °C for 15 min. .. Gene Expression Analysis Using a Quantitative Polymerase Chain Reaction (qPCR) Quantification of the gene expression of 3T3-L1 adipocytes and PWATs was performed using QuantStudio3 RT-PCR system (Applied Biosystems, Thermo Fisher Scientific, San Jose, CA, USA).

Isolation:

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Next, 2 µg of isolated total RNA was mixed with the Reverse Transcription Master premix kit (Elpis Bio, Daejeon, Korea), and the mixture was incubated for 60 min at 37 °C. .. The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Next, 2 µg of isolated total RNA was mixed with the Reverse Transcription Master premix kit (Elpis Bio, Daejeon, Korea), and the mixture was incubated for 60 min at 37 °C. .. The transcribed products were mixed with each primer set and Go Taq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

Article Title: Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes
Article Snippet: RNA Extraction and cDNA Synthesis Total RNA was isolated from the D3G-treated cells using an RNA isolation kit, according to the manufacturer’s instructions. .. RNA (50 ng) was converted into cDNA using a PCR machine (TaKaRa Bio, Kusatsu, Shiga, Japan) under the following conditions, which include initial incubation at 70 °C for 5 min followed by primer annealing at 25 °C for 5 min, extension at 42 °C for 60 min, and inactivation at 70 °C for 15 min.

Spectrophotometry:

Article Title: Mechanistic Studies of Anti-Hyperpigmentary Compounds: Elucidating Their Inhibitory and Regulatory Actions
Article Snippet: PCR machine was the product of Takara Bio Inc. (Otsu, Shiga, Japan). .. NanoDrop 2000c spectrophotometer for nucleic acid measurement was purchased from Thermo Scientific (Wilmington, DE, USA).

Mouse Assay:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: A total of 30 female mice, strain Balb/C, 3 months old, weight ranged from 30 g to 35 g, and 30 male mice, strain Balb/C, 4 months old, weight ranged from 40 g to 45 g, were collected from Veterinary Pharmacy Center, Surabaya, Indonesia. .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini).

Electrophoresis:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini). .. The female mice were induced with PMSG (Folligon) with a dosage of 5 IU and followed by 5 IU human chorionic gonadotropin (Chorulon) injection after 48 h. The female mice were mated with vasectomized male mice to induce ovulation.

Article Title: Association between Genetic Polymorphism of Multidrug Resistance 1 Gene and Sasang Constitutions
Article Snippet: The PCR reaction was amplified using a PCR machine (TAKARA, Japan). .. Electrophoresis of the reactant in 2% agarose gel confirmed the presence of the amplicons obtained from the PCR.

Marker:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: The materials utilized on this research were pregnant mare serum gonadotropin (PMSG) (Folligon® , Intervet, Boxmeer, Holland), human chorionic gonadotropin (Chorulon® , Intervet, Boxmeer, Holland), phosphate buffer saline (PBS), Dulbecco’s Medium Eagle’s Medium (Sigma® , St. Louis, USA), ethylene glycol (Sigma® , St. Louis, USA), propanediol (Sigma® , St. Louis, USA), mineral oil (Sigma® , St. Louis, USA), gentamicin sulfate, CO2 , lysis buffer to isolate the DNA, protease enzyme, constriction enzyme, chloroform (Merck), phenol (Merck), isoamyl alcohol (Merck), EDTA (Bioworld), PCR mix (Go Taq green Promega), primer (integrated DNA technologies), NaOH (Merck), bromophenol blue (Nacalai Tesque), urea (Promega), boric acid (Bioworld), SDS (Bioworld), DTT (Nacalai Tesque), agaroses (intron), formalin (Merck), silver nitrate (SAS), marker DNA 10kb (Intron), tetramethylethylenediamine (TEMED) (Wako), and APS (Nacalai Tesque). .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini).

Incubation:

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Next, 2 µg of isolated total RNA was mixed with the Reverse Transcription Master premix kit (Elpis Bio, Daejeon, Korea), and the mixture was incubated for 60 min at 37 °C. .. The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Next, 2 µg of isolated total RNA was mixed with the Reverse Transcription Master premix kit (Elpis Bio, Daejeon, Korea), and the mixture was incubated for 60 min at 37 °C. .. The transcribed products were mixed with each primer set and Go Taq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

Article Title: Anti-Adipogenic Effects of Delphinidin-3-O-β-Glucoside in 3T3-L1 Preadipocytes and Primary White Adipocytes
Article Snippet: .. RNA (50 ng) was converted into cDNA using a PCR machine (TaKaRa Bio, Kusatsu, Shiga, Japan) under the following conditions, which include initial incubation at 70 °C for 5 min followed by primer annealing at 25 °C for 5 min, extension at 42 °C for 60 min, and inactivation at 70 °C for 15 min. .. Gene Expression Analysis Using a Quantitative Polymerase Chain Reaction (qPCR) Quantification of the gene expression of 3T3-L1 adipocytes and PWATs was performed using QuantStudio3 RT-PCR system (Applied Biosystems, Thermo Fisher Scientific, San Jose, CA, USA).

CRAfT Assay:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini). .. The female mice were induced with PMSG (Folligon) with a dosage of 5 IU and followed by 5 IU human chorionic gonadotropin (Chorulon) injection after 48 h. The female mice were mated with vasectomized male mice to induce ovulation.

Inverted Microscopy:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini). .. The female mice were induced with PMSG (Folligon) with a dosage of 5 IU and followed by 5 IU human chorionic gonadotropin (Chorulon) injection after 48 h. The female mice were mated with vasectomized male mice to induce ovulation.

Lysis:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: The materials utilized on this research were pregnant mare serum gonadotropin (PMSG) (Folligon® , Intervet, Boxmeer, Holland), human chorionic gonadotropin (Chorulon® , Intervet, Boxmeer, Holland), phosphate buffer saline (PBS), Dulbecco’s Medium Eagle’s Medium (Sigma® , St. Louis, USA), ethylene glycol (Sigma® , St. Louis, USA), propanediol (Sigma® , St. Louis, USA), mineral oil (Sigma® , St. Louis, USA), gentamicin sulfate, CO2 , lysis buffer to isolate the DNA, protease enzyme, constriction enzyme, chloroform (Merck), phenol (Merck), isoamyl alcohol (Merck), EDTA (Bioworld), PCR mix (Go Taq green Promega), primer (integrated DNA technologies), NaOH (Merck), bromophenol blue (Nacalai Tesque), urea (Promega), boric acid (Bioworld), SDS (Bioworld), DTT (Nacalai Tesque), agaroses (intron), formalin (Merck), silver nitrate (SAS), marker DNA 10kb (Intron), tetramethylethylenediamine (TEMED) (Wako), and APS (Nacalai Tesque). .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini).

Sequencing:

Article Title: Association between Genetic Polymorphism of Multidrug Resistance 1 Gene and Sasang Constitutions
Article Snippet: Polymerase Chain Reaction and Restriction Fragment Length Polymorphism Analysis of MDR1 C1236T The SNPs were detected by sequence-specific primer polymerase chain reaction (SSP-PCR) using primer mixes ( ). .. The PCR reaction was amplified using a PCR machine (TAKARA, Japan).

Western Blot:

Article Title: Mechanistic Studies of Anti-Hyperpigmentary Compounds: Elucidating Their Inhibitory and Regulatory Actions
Article Snippet: Materials Apparatus for Western blot analysis was purchased from Bio-Rad (Hercules, CA, USA), except the film exposure cassette was from Amersham Biosciences Corporation (Piscataway, NJ, USA). .. PCR machine was the product of Takara Bio Inc. (Otsu, Shiga, Japan).

Real-time Polymerase Chain Reaction:

Article Title: The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method
Article Snippet: .. The instruments of this research were CO2 incubator (Thermo), inverted microscope (Nikon), real-time PCR, Tris (Bioworld), incubator (Memmert), vortex (Vortex mixer), centrifuge (Hettich), ethanol (Merck), acrylamide 30% (Nacalai Tesque), PCR machine (Takara), Tube 1.5 ml (Stardeck), vertical electrophoresis chamber (Bio Craft), PCR tube (Biologix), UV transilluminator (Bio-Rad), and spin-down (Force Mini). .. The female mice were induced with PMSG (Folligon) with a dosage of 5 IU and followed by 5 IU human chorionic gonadotropin (Chorulon) injection after 48 h. The female mice were mated with vasectomized male mice to induce ovulation.

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Paragraph title: Real-time PCR and reverse transcription (RT)-PCR ... The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Therapeutic potential of a phospholipase D1 inhibitory peptide fused with a cell-penetrating peptide as a novel anti-asthmatic drug in a Der f 2-induced airway inflammation model
Article Snippet: Paragraph title: Real-time PCR and reverse transcription (RT)-PCR ... The transcribed products were mixed with each primer set and GoTaq DNA polymerase (Promega, Madison, WI, USA) and were amplified in a PCR machine (Takara Bio).

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  • 90
    TaKaRa thermal cycler real time pcr machine
    HBO attenuates the expression of iNOS and nNOS, but not eNOS in the spinal dorsal horn following CCI Real-time <t>RT-PCR</t> analysis of iNOS, nNOS and eNOS mRNA in the spinal dorsal horn. Statistical results: (A) Relative iNOS mRNA expression <t>(iNOS/β-actin);</t> (B) Relative nNOS mRNA expression (nNOS/β-actin); (C) Relative eNOS mRNA expression (eNOS/β-actin). Data are shown as mean ± S.E.M, n= 6. * P
    Thermal Cycler Real Time Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermal cycler real time pcr machine/product/TaKaRa
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    thermal cycler real time pcr machine - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    79
    TaKaRa polymerase chain reaction pcr machine
    <t>PCR</t> amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) <t>cDNA.</t> PCR, polymerase chain reaction.
    Polymerase Chain Reaction Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction pcr machine/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polymerase chain reaction pcr machine - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    93
    TaKaRa real time pcr machine
    Administration of ATRES extract increased the mRNA expression of <t>IGF-1</t> on the dorsal skin area. Total RNA was isolated from the dorsal skin area of the mice treated with 40% ethanol (negative control), MXD (3%, w/v, positive control), the n -butanol (3%, w/v) extract, the DW (3%, w/v) extract, and the n -butanol-n4 fraction (3%, w/v). Total RNA was isolated from HaCaT cells treated with 40% ethanol (negative control) and ethanol extract. The relative levels of expression of IGF-1 ((a), (d)), KGF (b), and VEGF (c), normalized to beta actin, were determined by <t>qRT-PCR.</t> The data were analyzed by ANOVA, and the Student-Newman-Keuls test was then conducted. The data are presented as the means ± SD ( n = 3). ∗ indicates a statistically significant difference compared with the control group ( P
    Real Time Pcr Machine, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time pcr machine/product/TaKaRa
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    real time pcr machine - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    Image Search Results


    HBO attenuates the expression of iNOS and nNOS, but not eNOS in the spinal dorsal horn following CCI Real-time RT-PCR analysis of iNOS, nNOS and eNOS mRNA in the spinal dorsal horn. Statistical results: (A) Relative iNOS mRNA expression (iNOS/β-actin); (B) Relative nNOS mRNA expression (nNOS/β-actin); (C) Relative eNOS mRNA expression (eNOS/β-actin). Data are shown as mean ± S.E.M, n= 6. * P

    Journal: Oncotarget

    Article Title: Early hyperbaric oxygen effects on neuropathic pain and nitric oxide synthase isoforms in CCI rats

    doi: 10.18632/oncotarget.23867

    Figure Lengend Snippet: HBO attenuates the expression of iNOS and nNOS, but not eNOS in the spinal dorsal horn following CCI Real-time RT-PCR analysis of iNOS, nNOS and eNOS mRNA in the spinal dorsal horn. Statistical results: (A) Relative iNOS mRNA expression (iNOS/β-actin); (B) Relative nNOS mRNA expression (nNOS/β-actin); (C) Relative eNOS mRNA expression (eNOS/β-actin). Data are shown as mean ± S.E.M, n= 6. * P

    Article Snippet: Real-time PCR of NOS isoforms and β-actin was performed in Thermal Cycler Real-Time PCR machine (Takara, Kyoto, JPN) using the following program: 95°C for 30 s, 95°C for 5 s, 60°C for 30 s, and 72°C for 10 s. A total of 45 cycles were run followed by a melting curve analysis.

    Techniques: Expressing, Quantitative RT-PCR

    HBO attenuates the expression of iNOS and nNOS, but not eNOS in the spinal dorsal horn following CCI Real-time RT-PCR analysis of iNOS, nNOS and eNOS mRNA in the spinal dorsal horn. Statistical results: (A) Relative iNOS mRNA expression (iNOS/β-actin); (B) Relative nNOS mRNA expression (nNOS/β-actin); (C) Relative eNOS mRNA expression (eNOS/β-actin). Data are shown as mean ± S.E.M, n= 6. * P

    Journal: Oncotarget

    Article Title: Early hyperbaric oxygen effects on neuropathic pain and nitric oxide synthase isoforms in CCI rats

    doi: 10.18632/oncotarget.23867

    Figure Lengend Snippet: HBO attenuates the expression of iNOS and nNOS, but not eNOS in the spinal dorsal horn following CCI Real-time RT-PCR analysis of iNOS, nNOS and eNOS mRNA in the spinal dorsal horn. Statistical results: (A) Relative iNOS mRNA expression (iNOS/β-actin); (B) Relative nNOS mRNA expression (nNOS/β-actin); (C) Relative eNOS mRNA expression (eNOS/β-actin). Data are shown as mean ± S.E.M, n= 6. * P

    Article Snippet: Real-time PCR of NOS isoforms and β-actin was performed in Thermal Cycler Real-Time PCR machine (Takara, Kyoto, JPN) using the following program: 95°C for 30 s, 95°C for 5 s, 60°C for 30 s, and 72°C for 10 s. A total of 45 cycles were run followed by a melting curve analysis.

    Techniques: Expressing, Quantitative RT-PCR

    PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Journal: Molecular Medicine Reports

    Article Title: Lentivirus transduced interleukin-1 receptor antagonist gene expression in murine bone marrow-derived mesenchymal stem cells in vitro

    doi: 10.3892/mmr.2015.4003

    Figure Lengend Snippet: PCR amplified object fragment. The agarose gel electrophoresis revealed that the object band was in the expected position. M, DL2000 DNA marker; 1 and 2, PCR product (502 bp) cDNA. PCR, polymerase chain reaction.

    Article Snippet: The IL-1Ra cDNA was amplified using a polymerase chain reaction (PCR) machine (Takara Bio, Inc.) and the expression vector was cloned and validated by sequencing (Invitrogen Life Technologies).

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker

    Administration of ATRES extract increased the mRNA expression of IGF-1 on the dorsal skin area. Total RNA was isolated from the dorsal skin area of the mice treated with 40% ethanol (negative control), MXD (3%, w/v, positive control), the n -butanol (3%, w/v) extract, the DW (3%, w/v) extract, and the n -butanol-n4 fraction (3%, w/v). Total RNA was isolated from HaCaT cells treated with 40% ethanol (negative control) and ethanol extract. The relative levels of expression of IGF-1 ((a), (d)), KGF (b), and VEGF (c), normalized to beta actin, were determined by qRT-PCR. The data were analyzed by ANOVA, and the Student-Newman-Keuls test was then conducted. The data are presented as the means ± SD ( n = 3). ∗ indicates a statistically significant difference compared with the control group ( P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Extract of Allium tuberosum Rottler ex Spreng Promoted the Hair Growth through Regulating the Expression of IGF-1

    doi: 10.1155/2015/413538

    Figure Lengend Snippet: Administration of ATRES extract increased the mRNA expression of IGF-1 on the dorsal skin area. Total RNA was isolated from the dorsal skin area of the mice treated with 40% ethanol (negative control), MXD (3%, w/v, positive control), the n -butanol (3%, w/v) extract, the DW (3%, w/v) extract, and the n -butanol-n4 fraction (3%, w/v). Total RNA was isolated from HaCaT cells treated with 40% ethanol (negative control) and ethanol extract. The relative levels of expression of IGF-1 ((a), (d)), KGF (b), and VEGF (c), normalized to beta actin, were determined by qRT-PCR. The data were analyzed by ANOVA, and the Student-Newman-Keuls test was then conducted. The data are presented as the means ± SD ( n = 3). ∗ indicates a statistically significant difference compared with the control group ( P

    Article Snippet: Changes in the expression levels of IGF-1, VEGF, and KGF were calculated using a real-time PCR machine (2640A; Takara) as previously described [ ].

    Techniques: Expressing, Isolation, Mouse Assay, Negative Control, Positive Control, Quantitative RT-PCR