pcr gel  (Qiagen)


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    Structured Review

    Qiagen pcr gel
    Pcr Gel, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr gel/product/Qiagen
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr gel - by Bioz Stars, 2020-04
    87/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Total RNA was extracted from 50mg brain tissue using stratagen RNA extraction protocol and amplified by superscript II one step RT-PCR procedure from Invitrogen .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

    Amplification:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Total RNA was extracted from 50mg brain tissue using stratagen RNA extraction protocol and amplified by superscript II one step RT-PCR procedure from Invitrogen .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

    Polymerase Chain Reaction:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7). .. After analyzing the electropherogram for its nucleotide signals and purity, sequence text files were blasted with the M. fascicularies MDR1 coding region sequence (accession #AF537134) and M. nemestrinas MDR1 sequence was constructed by aligning both the forward and reverse primers.

    Construct:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Primate MDR1 cDNA Sequence for M. nemestrinas was constructed for sequence homology analysis with the human MDR1. .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

    Purification:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7). .. After analyzing the electropherogram for its nucleotide signals and purity, sequence text files were blasted with the M. fascicularies MDR1 coding region sequence (accession #AF537134) and M. nemestrinas MDR1 sequence was constructed by aligning both the forward and reverse primers.

    Sequencing:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7). .. After analyzing the electropherogram for its nucleotide signals and purity, sequence text files were blasted with the M. fascicularies MDR1 coding region sequence (accession #AF537134) and M. nemestrinas MDR1 sequence was constructed by aligning both the forward and reverse primers.

    Expressing:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Expression of possible mini and full length P-glycoprotein in the brain of M. nemestines . .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Total RNA was extracted from 50mg brain tissue using stratagen RNA extraction protocol and amplified by superscript II one step RT-PCR procedure from Invitrogen .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

    Activated Clotting Time Assay:

    Article Title: Identification and characterization of primate P-glycoprotein
    Article Snippet: Both forward and reverse primers were designed as pF11- AGT GTC CAG GTC GGA GCA AAG CGC CAG TGA A and pR11- TTC ACT GGC GCT TTG CTC CAG CCT GGA CAC T, based on M. fascicularies MDR1 cDNA sequence and acquired from Invitrogen . .. 3.2 pmol primers and 150 ng purified PCR gel (Qiagen kit) [ ] product were used for sequencing (ABI Prism, Model 3100, Version 3.7).

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    Qiagen gel based rt pcr
    Results of the <t>DENV</t> <t>RT-PCR</t> assay performed on serum samples obtained 1 to 9 days after onset of symptoms. (A) Archived serum samples that had been collected from 60 patients on days 1–3 (n = 5), 4 (n = 10), 5 (n = 7), 6 (n = 11), 7 (n = 12), 8 (n = 8), and 9 (n = 7) after disease onset were tested by the DENV RT-PCR assay, an NS1 antigen detection test, IgM capture ELISA, and IFA detecting DENV-specific IgG antibodies. The criteria for a positive result in each of these analyses are explained in Methods . The curves show the percent of samples testing positive in the individual assays for the specified days after disease onset. (B) Viral load in samples collected on days 1–9 after onset of symptoms. Each dot represents the mean of results for duplicate samples from a single patient. GCE = genome copy equivalents.
    Gel Based Rt Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel based rt pcr/product/Qiagen
    Average 86 stars, based on 2 article reviews
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    gel based rt pcr - by Bioz Stars, 2020-04
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    92
    Qiagen gel purified pcr
    Relative expression of maeK , <t>maeE</t> , maeP , and maeR in ABSA 834 and UPSA 807 following growth in 12.5-ml cultures of synthetic human urine (SHU). <t>qRT-PCR</t> data show fold change relative to the reference rpoB in the presence and absence of 40 mM malic acid.
    Gel Purified Pcr, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gel purified pcr/product/Qiagen
    Average 92 stars, based on 15 article reviews
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    86
    Qiagen polymerase chain reaction denaturing gradient gel electrophoresis
    Bacterial community of weaned piglets fed diet supplemented with antibiotics or B. subtilis . (a) DGGE profiles of the V6 to V8 regions of the 16S rDNA gene fragments from the samples. The denaturant <t>gradient</t> range is 42% to 58% and the major difference bands are numbered. Lane S (Standard ladder, which indicates PCR products generated from different bacterial 16S rDNA genes with primers 968F-GC and 1401R); NC (negative control, basal diet); PC (positive control, diet supplemented with antibiotics); L, M, H (diets supplemented with probiotics 2×10 9 , 4×10 9 , and 20×10 9 CFU/kg feed, respectively); (b) Unweighted pair-group method with arithmetic means (UPGMA) analysis of Dice similarity indices from DGGE profiles. DGGE, <t>denaturing</t> gradient <t>gel</t> <t>electrophoresis;</t> PCR, <t>polymerase</t> <t>chain</t> <t>reaction.</t>
    Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polymerase chain reaction denaturing gradient gel electrophoresis/product/Qiagen
    Average 86 stars, based on 1 article reviews
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    Image Search Results


    Results of the DENV RT-PCR assay performed on serum samples obtained 1 to 9 days after onset of symptoms. (A) Archived serum samples that had been collected from 60 patients on days 1–3 (n = 5), 4 (n = 10), 5 (n = 7), 6 (n = 11), 7 (n = 12), 8 (n = 8), and 9 (n = 7) after disease onset were tested by the DENV RT-PCR assay, an NS1 antigen detection test, IgM capture ELISA, and IFA detecting DENV-specific IgG antibodies. The criteria for a positive result in each of these analyses are explained in Methods . The curves show the percent of samples testing positive in the individual assays for the specified days after disease onset. (B) Viral load in samples collected on days 1–9 after onset of symptoms. Each dot represents the mean of results for duplicate samples from a single patient. GCE = genome copy equivalents.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections

    doi: 10.1371/journal.pntd.0003416

    Figure Lengend Snippet: Results of the DENV RT-PCR assay performed on serum samples obtained 1 to 9 days after onset of symptoms. (A) Archived serum samples that had been collected from 60 patients on days 1–3 (n = 5), 4 (n = 10), 5 (n = 7), 6 (n = 11), 7 (n = 12), 8 (n = 8), and 9 (n = 7) after disease onset were tested by the DENV RT-PCR assay, an NS1 antigen detection test, IgM capture ELISA, and IFA detecting DENV-specific IgG antibodies. The criteria for a positive result in each of these analyses are explained in Methods . The curves show the percent of samples testing positive in the individual assays for the specified days after disease onset. (B) Viral load in samples collected on days 1–9 after onset of symptoms. Each dot represents the mean of results for duplicate samples from a single patient. GCE = genome copy equivalents.

    Article Snippet: Gel-based RT-PCR and sequencing DENV-specific RNA was amplified in 25-µL reaction mixtures by primer pairs 5′-TCAATATGCTGAAACGCGHGAG-3′ and 5′-GCGCCTTCNGNNGACATCCA-3′ using a OneStep RT-PCR kit (Qiagen).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunofluorescence

    Overview of the primers and probe in the DENV RT-PCR assay. The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serotypes 1–3 and serotype 4, respectively. Vertical bars and percentages show the fraction of sequences with nucleotides deviating from the consensus of DENV serotypes 1–3 (A) and serotype 4 (B). Percentages below 1 are not shown. Numbers indicate genomic positions.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections

    doi: 10.1371/journal.pntd.0003416

    Figure Lengend Snippet: Overview of the primers and probe in the DENV RT-PCR assay. The reverse primers DENV_R1–3 (A) and DENV_R4 (B) were specifically designed to target DENV serotypes 1–3 and serotype 4, respectively. Vertical bars and percentages show the fraction of sequences with nucleotides deviating from the consensus of DENV serotypes 1–3 (A) and serotype 4 (B). Percentages below 1 are not shown. Numbers indicate genomic positions.

    Article Snippet: Gel-based RT-PCR and sequencing DENV-specific RNA was amplified in 25-µL reaction mixtures by primer pairs 5′-TCAATATGCTGAAACGCGHGAG-3′ and 5′-GCGCCTTCNGNNGACATCCA-3′ using a OneStep RT-PCR kit (Qiagen).

    Techniques: Reverse Transcription Polymerase Chain Reaction

    Dynamic range and limit of detection of the DENV RT-PCR assay. (A) The linear dynamic range of the DENV RT-PCR assay was determined by testing triplicates of 10-fold serially diluted in vitro transcribed RNA. The sequences of the transcript RNA (RNA[DENV_R1–3] and RNA[DENV_R4]) were matched with the two reverse primers DENV_R1–3 and DENV_R4, respectively. Each dot represents the mean Cq-value from three replicates, the error bars indicate the 95% confidence interval, and the lines illustrate the result of the lin-log regression analysis. (B and C) Limit of detection was determined by assaying eight replicates of twofold serially diluted RNA transcripts in three separate experiments, and the results of testing are shown for RNA[DENV_R1–3] (B) and RNA[DENV_R4] (C). Horizontal lines indicate mean values, boxes denote the 25th to 75th percentiles and whiskers the 5–95% percentiles, and dots represent outliers. The number of positives per total number of replicates tested is given above each box. Limit of detection was defined as the last dilution in which transcript RNA was detected in all 24 replicates. GCE = genome copy equivalents.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections

    doi: 10.1371/journal.pntd.0003416

    Figure Lengend Snippet: Dynamic range and limit of detection of the DENV RT-PCR assay. (A) The linear dynamic range of the DENV RT-PCR assay was determined by testing triplicates of 10-fold serially diluted in vitro transcribed RNA. The sequences of the transcript RNA (RNA[DENV_R1–3] and RNA[DENV_R4]) were matched with the two reverse primers DENV_R1–3 and DENV_R4, respectively. Each dot represents the mean Cq-value from three replicates, the error bars indicate the 95% confidence interval, and the lines illustrate the result of the lin-log regression analysis. (B and C) Limit of detection was determined by assaying eight replicates of twofold serially diluted RNA transcripts in three separate experiments, and the results of testing are shown for RNA[DENV_R1–3] (B) and RNA[DENV_R4] (C). Horizontal lines indicate mean values, boxes denote the 25th to 75th percentiles and whiskers the 5–95% percentiles, and dots represent outliers. The number of positives per total number of replicates tested is given above each box. Limit of detection was defined as the last dilution in which transcript RNA was detected in all 24 replicates. GCE = genome copy equivalents.

    Article Snippet: Gel-based RT-PCR and sequencing DENV-specific RNA was amplified in 25-µL reaction mixtures by primer pairs 5′-TCAATATGCTGAAACGCGHGAG-3′ and 5′-GCGCCTTCNGNNGACATCCA-3′ using a OneStep RT-PCR kit (Qiagen).

    Techniques: Reverse Transcription Polymerase Chain Reaction, In Vitro

    Flow-chart showing the laboratory test results for samples from returning travelers with dengue-compatible symptomatology. Serum samples collected during January and February 2014 were tested consecutively by the newly developed DENV RT-PCR method, an NS1 antigen detection test, IgM capture ELISA, and/or an in-house IFA detecting DENV-specific IgG antibodies. The criteria for a positive result in each individual assay are explained in Methods . The results of the laboratory analysis of the first sample arriving at the Public Health Agency of Sweden are shown. w/o = without.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: Universal Single-Probe RT-PCR Assay for Diagnosis of Dengue Virus Infections

    doi: 10.1371/journal.pntd.0003416

    Figure Lengend Snippet: Flow-chart showing the laboratory test results for samples from returning travelers with dengue-compatible symptomatology. Serum samples collected during January and February 2014 were tested consecutively by the newly developed DENV RT-PCR method, an NS1 antigen detection test, IgM capture ELISA, and/or an in-house IFA detecting DENV-specific IgG antibodies. The criteria for a positive result in each individual assay are explained in Methods . The results of the laboratory analysis of the first sample arriving at the Public Health Agency of Sweden are shown. w/o = without.

    Article Snippet: Gel-based RT-PCR and sequencing DENV-specific RNA was amplified in 25-µL reaction mixtures by primer pairs 5′-TCAATATGCTGAAACGCGHGAG-3′ and 5′-GCGCCTTCNGNNGACATCCA-3′ using a OneStep RT-PCR kit (Qiagen).

    Techniques: Flow Cytometry, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Immunofluorescence

    Relative expression of maeK , maeE , maeP , and maeR in ABSA 834 and UPSA 807 following growth in 12.5-ml cultures of synthetic human urine (SHU). qRT-PCR data show fold change relative to the reference rpoB in the presence and absence of 40 mM malic acid.

    Journal: Infection and Immunity

    Article Title: Discovery and Characterization of Human-Urine Utilization by Asymptomatic-Bacteriuria-Causing Streptococcus agalactiae

    doi: 10.1128/IAI.00938-15

    Figure Lengend Snippet: Relative expression of maeK , maeE , maeP , and maeR in ABSA 834 and UPSA 807 following growth in 12.5-ml cultures of synthetic human urine (SHU). qRT-PCR data show fold change relative to the reference rpoB in the presence and absence of 40 mM malic acid.

    Article Snippet: The constructs for mutation of maeE and maeK were generated by introducing gel-purified PCR products (QIAquick gel extraction kit; Qiagen) into pHY304-aad9 using restriction sites listed in Table S1 and designated pDI102 and pGU2436, respectively.

    Techniques: Expressing, Quantitative RT-PCR

    Bacterial community of weaned piglets fed diet supplemented with antibiotics or B. subtilis . (a) DGGE profiles of the V6 to V8 regions of the 16S rDNA gene fragments from the samples. The denaturant gradient range is 42% to 58% and the major difference bands are numbered. Lane S (Standard ladder, which indicates PCR products generated from different bacterial 16S rDNA genes with primers 968F-GC and 1401R); NC (negative control, basal diet); PC (positive control, diet supplemented with antibiotics); L, M, H (diets supplemented with probiotics 2×10 9 , 4×10 9 , and 20×10 9 CFU/kg feed, respectively); (b) Unweighted pair-group method with arithmetic means (UPGMA) analysis of Dice similarity indices from DGGE profiles. DGGE, denaturing gradient gel electrophoresis; PCR, polymerase chain reaction.

    Journal: Asian-Australasian Journal of Animal Sciences

    Article Title: Effects of Bacillus subtilis KN-42 on Growth Performance, Diarrhea and Faecal Bacterial Flora of Weaned Piglets

    doi: 10.5713/ajas.2013.13737

    Figure Lengend Snippet: Bacterial community of weaned piglets fed diet supplemented with antibiotics or B. subtilis . (a) DGGE profiles of the V6 to V8 regions of the 16S rDNA gene fragments from the samples. The denaturant gradient range is 42% to 58% and the major difference bands are numbered. Lane S (Standard ladder, which indicates PCR products generated from different bacterial 16S rDNA genes with primers 968F-GC and 1401R); NC (negative control, basal diet); PC (positive control, diet supplemented with antibiotics); L, M, H (diets supplemented with probiotics 2×10 9 , 4×10 9 , and 20×10 9 CFU/kg feed, respectively); (b) Unweighted pair-group method with arithmetic means (UPGMA) analysis of Dice similarity indices from DGGE profiles. DGGE, denaturing gradient gel electrophoresis; PCR, polymerase chain reaction.

    Article Snippet: DNA extraction and polymerase chain reaction-denaturing gradient gel electrophoresis The DNA from faecal samples was extracted with the QIAamp DNA Stool Mini Kit (Qiagen, Duesseldorf, Germany) according to the manufacturer’s instructions, at 95°C for the initial lysis step ( ).

    Techniques: Denaturing Gradient Gel Electrophoresis, Polymerase Chain Reaction, Generated, Negative Control, Positive Control