pcr cleanup kit  (Thermo Fisher)


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    Name:
    ChargeSwitch PCR Clean Up Kit
    Description:
    • Sample size 25 50 µl• High recovery of DNA fragments• Rapid one tube 5 minute protocolChargeSwitch PCR Clean Up Kit is the fastest and most effective method of removing primers unincorporated dNTPs thermostable polymerases and salts Figure 1 This kit is scalable for use on liquid handling robots This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory Please see the Accessories Chapter Chapter 6 to select the best magnetic accessory for your needs
    Catalog Number:
    cs12000
    Price:
    None
    Applications:
    DNA & RNA Purification & Analysis|DNA Extraction|PCR Product Clean-Up
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher pcr cleanup kit
    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious <t>DNA.</t> CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV <t>PCR</t> products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.
    • Sample size 25 50 µl• High recovery of DNA fragments• Rapid one tube 5 minute protocolChargeSwitch PCR Clean Up Kit is the fastest and most effective method of removing primers unincorporated dNTPs thermostable polymerases and salts Figure 1 This kit is scalable for use on liquid handling robots This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory Please see the Accessories Chapter Chapter 6 to select the best magnetic accessory for your needs
    https://www.bioz.com/result/pcr cleanup kit/product/Thermo Fisher
    Average 90 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    pcr cleanup kit - by Bioz Stars, 2020-07
    90/100 stars

    Images

    1) Product Images from "A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput"

    Article Title: A Simplified Positive-Sense-RNA Virus Construction Approach That Enhances Analysis Throughput

    Journal: Journal of Virology

    doi: 10.1128/JVI.02261-13

    Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious DNA. CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV PCR products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.
    Figure Legend Snippet: Construction of DENV by Gibson assembly. (A) Assembly schemes of DENV infectious DNA. CMV, CMV promoter; HDV, HDV ribozyme; SV40PA, polyadenylation signal. (Left) Assembly scheme for DENV2-16681 and DENV4-H241; (right) assembly scheme for DENV4-v17. (B) The comparison between the foci of the original virus stocks and the foci of viruses recovered through Gibson assembly. The number of DENV PCR products assembled (# PCR) is shown. (C) PCR products for sequencing of virus recovered from transfected 293T cells were generated from cDNA and not contaminated assembled DNA. +, viral RNA was treated with RNase A during cDNA synthesis. (D) Comparison between sequence heterogeneities of the original v17 strain, v17 recovered by our method, and v17 cultured in C6/36 cells. The heterogeneous positions are highlighted in yellow.

    Techniques Used: Polymerase Chain Reaction, Sequencing, Transfection, Generated, Cell Culture

    Construction of a DENV library with randomized amino acids at position E1690. (A) Diagram showing how the randomization was achieved at the assembly site of two PCR products (dark gray and black) during Gibson ligation. The primer sites for PCR are depicted in letters, while the rest of DNA strands are represented by solid lines. The arrows represent the direction of repair by Phusion polymerase during the assembly reaction. (B) Chromatograms of sequences at prM847, E1690, and NS4B7016 of prM+E+NS4B virus with amino acid randomization at E1690. The randomized position in E and heterogeneous nucleotides in M and NS4B are highlighted in yellow. (C) Comparison between the foci of the DENV library (prM+LibE+NS4B) and the foci of the virus with the same genetic background without randomization at E1690 (prM+E+NS4B). The foci for each virus stock in duplicate wells are shown.
    Figure Legend Snippet: Construction of a DENV library with randomized amino acids at position E1690. (A) Diagram showing how the randomization was achieved at the assembly site of two PCR products (dark gray and black) during Gibson ligation. The primer sites for PCR are depicted in letters, while the rest of DNA strands are represented by solid lines. The arrows represent the direction of repair by Phusion polymerase during the assembly reaction. (B) Chromatograms of sequences at prM847, E1690, and NS4B7016 of prM+E+NS4B virus with amino acid randomization at E1690. The randomized position in E and heterogeneous nucleotides in M and NS4B are highlighted in yellow. (C) Comparison between the foci of the DENV library (prM+LibE+NS4B) and the foci of the virus with the same genetic background without randomization at E1690 (prM+E+NS4B). The foci for each virus stock in duplicate wells are shown.

    Techniques Used: Polymerase Chain Reaction, Ligation

    Related Articles

    Amplification:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. The resulting trimmed data set was analysed using a pipeline created at the Alkek Center for Metagenomics and Microbiome Research at Baylor College of Medicine .

    Construct:

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. The resulting trimmed data set was analysed using a pipeline created at the Alkek Center for Metagenomics and Microbiome Research at Baylor College of Medicine .

    Purification:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: The dynamic DNA methylation landscape of the mutL homolog 1 shore is altered by MLH1-93G > A polymorphism in normal tissues and colorectal cancer
    Article Snippet: .. PCR product was purified using ChargeSwitch PCR Clean-Up Kit (Invitrogen). .. PCR products were cloned using the pGEM-T Easy Vector System (Promega, Madison, WI) and MAX Efficiency DH5α Competent Cells (Life Technologies, Carlsbad, CA) according to manufacturer’s protocol.

    Article Title: Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment
    Article Snippet: .. PCR products were purified using the ChargeSwitch PCR Clean-Up Kit (Invitrogen) followed by restriction digestion. ..

    Article Title: Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium
    Article Snippet: .. PCR products were purified with the ChargeSwitch® PCR Clean-Up kit (Life Technologies) and subsequently sequenced by the Ion Torrent Personal Genome Machine System (PGM) available at the Microbiology Laboratory of the National Centre for Environmental Research, Assessment and Impact Evaluation—EMBRAPA (Jaguariúna, São Paulo, Brazil). .. Sequence analysis was conducted using QIIME (Quantitative Insights into Microbial Ecology) software [ ].

    Article Title: Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil
    Article Snippet: .. DNA templates were extracted by the thermal lysis method [ ] and purified using the commercial kit ChargeSwitch® PCR Clean-up kit (Invitrogen, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: A Set of Multiplex Polymerase Chain Reactions for Genomic Detection of Nine Edible Insect Species in Foods
    Article Snippet: .. Successively, amplicons were purified using a commercial purification kit (ChargeSwitch PCR Clean-Up Kit, Invitrogen, Grand Island, NY) and sequenced (ABI Prism 310 Genetic Analyser, Applied Biosystems, Foster City, CA) to confirm the identity of the insect species and of the PCR amplified products. ..

    Article Title: The dynamic DNA methylation landscape of the mutL homolog 1 shore is altered by MLH1-93G > A polymorphism in normal tissues and colorectal cancer
    Article Snippet: .. PCR product was purified using ChargeSwitch PCR Clean-Up Kit (Invitrogen). .. PCR products were cloned using the pGEM-T Easy Vector System (Promega, Madison, WI) and MAX Efficiency DH5α Competent Cells (Life Technologies, Carlsbad, CA) according to manufacturer’s protocol.

    Article Title: Biosynthesis of the Escherichia coli K1 group 2 polysialic acid capsule occurs within a protected cytoplasmic compartment
    Article Snippet: .. PCR products were purified using the ChargeSwitch PCR Clean-Up Kit (Invitrogen) followed by restriction digestion. ..

    Article Title: Shifts in the bacterial community composition along deep soil profiles in monospecific and mixed stands of Eucalyptus grandis and Acacia mangium
    Article Snippet: .. PCR products were purified with the ChargeSwitch® PCR Clean-Up kit (Life Technologies) and subsequently sequenced by the Ion Torrent Personal Genome Machine System (PGM) available at the Microbiology Laboratory of the National Centre for Environmental Research, Assessment and Impact Evaluation—EMBRAPA (Jaguariúna, São Paulo, Brazil). .. Sequence analysis was conducted using QIIME (Quantitative Insights into Microbial Ecology) software [ ].

    Article Title: Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil
    Article Snippet: .. DNA templates were extracted by the thermal lysis method [ ] and purified using the commercial kit ChargeSwitch® PCR Clean-up kit (Invitrogen, CA, USA). ..

    Article Title: Seasonal and ontogenetic variation of skin microbial communities and relationships to natural disease dynamics in declining amphibians
    Article Snippet: .. We pooled 50 ng of all PCR products into a single vial and cleaned it using ChargeSwitch PCR clean-up kit (Invitrogen, Inc.) before sequencing. ..

    Random Hexamer Labeling:

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit. .. The resulting viral amplicons were normalized, pooled, and made into an Illumina library without shearing.

    Article Title: Multi-omics of the gut microbial ecosystem in inflammatory bowel diseases
    Article Snippet: .. After short molecule and random hexamer removal using ChargeSwitch (CS12000, Thermo Fisher), molecules were amplified and tagged with a BC12-V8A2 construct using AccuPrimeTM Taq polymerase and cleaned with ChargeSwitch kit.). .. The resulting trimmed data set was analysed using a pipeline created at the Alkek Center for Metagenomics and Microbiome Research at Baylor College of Medicine .

    Sequencing:

    Article Title: Seasonal and ontogenetic variation of skin microbial communities and relationships to natural disease dynamics in declining amphibians
    Article Snippet: .. We pooled 50 ng of all PCR products into a single vial and cleaned it using ChargeSwitch PCR clean-up kit (Invitrogen, Inc.) before sequencing. ..

    Lysis:

    Article Title: Molecular Typing of Mycobacterium bovis from Cattle Reared in Midwest Brazil
    Article Snippet: .. DNA templates were extracted by the thermal lysis method [ ] and purified using the commercial kit ChargeSwitch® PCR Clean-up kit (Invitrogen, CA, USA). ..

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  • 96
    Thermo Fisher dna cleanup micro kit
    <t>DNA</t> gyrase behavior depends on a DNA substrate gyrase binding score. ( A ) EMSA analysis of DNA gyrase binding with 133 bp fragments; fragments scores are showed above the pictures; molar gyrase/DNA ratio is indicated under the lanes. SC—scrambled consensus, C—consensus. ( B ) Time-course of gyrase supercoiling of <t>pUC19</t> plasmids harboring indicated 133 bp fragments; RE—relaxed plasmid, NSC—negatively supercoiled plasmid. 1—scrambled consensus, 2—Mu SGS, 3—consensus. ( C ) Time-course of ATP-independent relaxation of pUC19 plasmids harbouring indicated 133 bp fragments. ( D ) Time-course of ATP-dependent relaxation of the same plasmids by truncated GyrA59 2 GyrB 2 gyrase lacking CTD.
    Dna Cleanup Micro Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna cleanup micro kit/product/Thermo Fisher
    Average 96 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    dna cleanup micro kit - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    DNA gyrase behavior depends on a DNA substrate gyrase binding score. ( A ) EMSA analysis of DNA gyrase binding with 133 bp fragments; fragments scores are showed above the pictures; molar gyrase/DNA ratio is indicated under the lanes. SC—scrambled consensus, C—consensus. ( B ) Time-course of gyrase supercoiling of pUC19 plasmids harboring indicated 133 bp fragments; RE—relaxed plasmid, NSC—negatively supercoiled plasmid. 1—scrambled consensus, 2—Mu SGS, 3—consensus. ( C ) Time-course of ATP-independent relaxation of pUC19 plasmids harbouring indicated 133 bp fragments. ( D ) Time-course of ATP-dependent relaxation of the same plasmids by truncated GyrA59 2 GyrB 2 gyrase lacking CTD.

    Journal: Nucleic Acids Research

    Article Title: Single-nucleotide-resolution mapping of DNA gyrase cleavage sites across the Escherichia coli genome

    doi: 10.1093/nar/gky1222

    Figure Lengend Snippet: DNA gyrase behavior depends on a DNA substrate gyrase binding score. ( A ) EMSA analysis of DNA gyrase binding with 133 bp fragments; fragments scores are showed above the pictures; molar gyrase/DNA ratio is indicated under the lanes. SC—scrambled consensus, C—consensus. ( B ) Time-course of gyrase supercoiling of pUC19 plasmids harboring indicated 133 bp fragments; RE—relaxed plasmid, NSC—negatively supercoiled plasmid. 1—scrambled consensus, 2—Mu SGS, 3—consensus. ( C ) Time-course of ATP-independent relaxation of pUC19 plasmids harbouring indicated 133 bp fragments. ( D ) Time-course of ATP-dependent relaxation of the same plasmids by truncated GyrA59 2 GyrB 2 gyrase lacking CTD.

    Article Snippet: For EMSA and competition assays DNA fragments were PCR amplified using pUC19_for and pUC19_rev primers ( ) and purified with Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific).

    Techniques: Binding Assay, Plasmid Preparation