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Fisher Scientific pcr cleanup kit
Pcr Cleanup Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr cleanup kit/product/Fisher Scientific
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pcr cleanup kit - by Bioz Stars, 2020-07
93/100 stars

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Polymerase Chain Reaction:

Article Title: Using a soil bacterial species balance index to estimate potato crop productivity
Article Snippet: .. The PCR reaction was purified using the Axygen PCR cleanup kit (Fisher Scientific, Nepean, ON, Canada). ..

Purification:

Article Title: Using a soil bacterial species balance index to estimate potato crop productivity
Article Snippet: .. The PCR reaction was purified using the Axygen PCR cleanup kit (Fisher Scientific, Nepean, ON, Canada). ..

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    Fisher Scientific rna cleanup kit
    <t>MCPIP1</t> selectively inhibits the mRNA expression of anti-apoptotic genes. (A) Clustergram of the apoptosis related genes affected differentially in MDA-MB-231/Tet-On cells at 32 hours after adding Dox (500 ng/ml). MCPIP1: cells with Dox; Control: cells without Dox. MDA-MB-231/Tet-On cells were induced to express MCPIP1 by Dox (500 ng/ml) for different times. Then total <t>RNA</t> was collected to detect mRNA levels of selected anti-apoptotic genes by qRT-PCR, including bcl2l1 (B), bric3 (C), bcl3 (D), and relb (E). The mRNA levels of pro-apoptotic genes in the above cells were measured by qRT-PCR, including bad (F), ripk2 (G), fas (H), and dedd2 (I). MDA-MB-231/Tet-On cells were induced to express GFP/MCPIP1 fusion protein by Dox (500 ng/ml) for 36 h, followed by lysate extraction, immunoprecipitation with anti-GFP antibody or IgG, and RNA extraction. RT-PCR was performed to detect anti-apoptotic gene transcripts (J) and pro-apoptotic gene transcripts (K). IL-6 transcript was used as positive control and GAPDH served as negative control.
    Rna Cleanup Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna cleanup kit/product/Fisher Scientific
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna cleanup kit - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Fisher Scientific pcr cleanup kit
    <t>MCPIP1</t> selectively inhibits the mRNA expression of anti-apoptotic genes. (A) Clustergram of the apoptosis related genes affected differentially in MDA-MB-231/Tet-On cells at 32 hours after adding Dox (500 ng/ml). MCPIP1: cells with Dox; Control: cells without Dox. MDA-MB-231/Tet-On cells were induced to express MCPIP1 by Dox (500 ng/ml) for different times. Then total <t>RNA</t> was collected to detect mRNA levels of selected anti-apoptotic genes by qRT-PCR, including bcl2l1 (B), bric3 (C), bcl3 (D), and relb (E). The mRNA levels of pro-apoptotic genes in the above cells were measured by qRT-PCR, including bad (F), ripk2 (G), fas (H), and dedd2 (I). MDA-MB-231/Tet-On cells were induced to express GFP/MCPIP1 fusion protein by Dox (500 ng/ml) for 36 h, followed by lysate extraction, immunoprecipitation with anti-GFP antibody or IgG, and RNA extraction. RT-PCR was performed to detect anti-apoptotic gene transcripts (J) and pro-apoptotic gene transcripts (K). IL-6 transcript was used as positive control and GAPDH served as negative control.
    Pcr Cleanup Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr cleanup kit/product/Fisher Scientific
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr cleanup kit - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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    MCPIP1 selectively inhibits the mRNA expression of anti-apoptotic genes. (A) Clustergram of the apoptosis related genes affected differentially in MDA-MB-231/Tet-On cells at 32 hours after adding Dox (500 ng/ml). MCPIP1: cells with Dox; Control: cells without Dox. MDA-MB-231/Tet-On cells were induced to express MCPIP1 by Dox (500 ng/ml) for different times. Then total RNA was collected to detect mRNA levels of selected anti-apoptotic genes by qRT-PCR, including bcl2l1 (B), bric3 (C), bcl3 (D), and relb (E). The mRNA levels of pro-apoptotic genes in the above cells were measured by qRT-PCR, including bad (F), ripk2 (G), fas (H), and dedd2 (I). MDA-MB-231/Tet-On cells were induced to express GFP/MCPIP1 fusion protein by Dox (500 ng/ml) for 36 h, followed by lysate extraction, immunoprecipitation with anti-GFP antibody or IgG, and RNA extraction. RT-PCR was performed to detect anti-apoptotic gene transcripts (J) and pro-apoptotic gene transcripts (K). IL-6 transcript was used as positive control and GAPDH served as negative control.

    Journal: Cancer research

    Article Title: MCPIP1 selectively destabilizes transcripts associated with an anti-apoptotic gene expression program in breast cancer cells that can elicit complete tumor regression

    doi: 10.1158/0008-5472.CAN-15-1115

    Figure Lengend Snippet: MCPIP1 selectively inhibits the mRNA expression of anti-apoptotic genes. (A) Clustergram of the apoptosis related genes affected differentially in MDA-MB-231/Tet-On cells at 32 hours after adding Dox (500 ng/ml). MCPIP1: cells with Dox; Control: cells without Dox. MDA-MB-231/Tet-On cells were induced to express MCPIP1 by Dox (500 ng/ml) for different times. Then total RNA was collected to detect mRNA levels of selected anti-apoptotic genes by qRT-PCR, including bcl2l1 (B), bric3 (C), bcl3 (D), and relb (E). The mRNA levels of pro-apoptotic genes in the above cells were measured by qRT-PCR, including bad (F), ripk2 (G), fas (H), and dedd2 (I). MDA-MB-231/Tet-On cells were induced to express GFP/MCPIP1 fusion protein by Dox (500 ng/ml) for 36 h, followed by lysate extraction, immunoprecipitation with anti-GFP antibody or IgG, and RNA extraction. RT-PCR was performed to detect anti-apoptotic gene transcripts (J) and pro-apoptotic gene transcripts (K). IL-6 transcript was used as positive control and GAPDH served as negative control.

    Article Snippet: Total RNA was extracted with Trizol from MCPIP1-expressed and -unexpressed MDA-MB-231 cells, purified using the RNA Cleanup Kit (Fisher Scientific), reverse-transcribed to cDNA, and added to each well of the PCR Array plates combined with TaqMan Master Mix according to the manufacturer's instructions.

    Techniques: Expressing, Multiple Displacement Amplification, Quantitative RT-PCR, Immunoprecipitation, RNA Extraction, Reverse Transcription Polymerase Chain Reaction, Positive Control, Negative Control