pcr clean up  (Thermo Fisher)


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    Name:
    ExoSAP IT Express PCR Product Cleanup Reagent
    Description:
    In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
    Catalog Number:
    75001.1.ea
    Price:
    None
    Applications:
    PCR|PCR & Real-Time PCR|Sequencing
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher pcr clean up
    In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
    https://www.bioz.com/result/pcr clean up/product/Thermo Fisher
    Average 99 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S]
    Article Snippet: .. PCR products of positive clones were cleaned with ExoSAP-it (Affymetrix) and sequenced using BigDye Terminator v3.1 reagent (Applied Biosystems) at the sequencing facility of the University of Bergen. .. In situ hybridization To confirm the in situ hybridization specificity, two different single stranded digoxigenin (DIG)-labeled RNA probes of 476 bp and 604 bp lengths corresponding to different regions of LsMTP transcripts ( ) were synthesized separately from cDNA using the DIG RNA labeling kit (Roche).

    Polymerase Chain Reaction:

    Article Title: Association of FOXP3 Single Nucleotide Polymorphisms With Clinical Outcomes After Allogenic Hematopoietic Stem Cell Transplantation
    Article Snippet: .. Next, 2 µL ExoSAP-IT PCR Clean Up (Affymetrix, Santa Clara, CA, USA) was added to 5 µL of PCR product, followed by incubation at 37℃ for 15 minutes and at 80℃ for 15 minutes. .. We then added 1 µL of 5 pmol/µL sequencing primer, 4 µL of deionized water, and 4 µL of BigDye Terminator Ready Reaction Mix (Life Technologies, Grand Island, NY, USA) to 1 µL of purified PCR product.

    Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S]
    Article Snippet: .. PCR products of positive clones were cleaned with ExoSAP-it (Affymetrix) and sequenced using BigDye Terminator v3.1 reagent (Applied Biosystems) at the sequencing facility of the University of Bergen. .. In situ hybridization To confirm the in situ hybridization specificity, two different single stranded digoxigenin (DIG)-labeled RNA probes of 476 bp and 604 bp lengths corresponding to different regions of LsMTP transcripts ( ) were synthesized separately from cDNA using the DIG RNA labeling kit (Roche).

    Article Title: Use of Phage Display to Identify Novel Mineralocorticoid Receptor-Interacting Proteins
    Article Snippet: .. PCR products were purified using ExoSap-IT according to the manufacturer's protocol (Affymetrix/USB; number 78200). .. Five microliters of the PCR product and 1 μL of ExoSap-IT was mixed and incubated at 37°C for 30 minutes.

    Article Title: A New PCR-Based Method Shows That Blue Crabs (Callinectes sapidus (Rathbun)) Consume Winter Flounder (Pseudopleuronectes americanus (Walbaum))
    Article Snippet: .. DNA Sequencing Selected PCR products were treated with ExoSAP-It in accordance with the manufacturer’s protocol (Affymetrix, Santa Clara, CA, USA), combined with an appropriate primer and submitted to the Stony Brook University DNA Sequencing Facility. ..

    Mutagenesis:

    Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma
    Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by ExoSAP-IT treatment (USB, Cleveland, OH), sequenced using Big Dye Termination chemistry (Applied Biosystems, Weiterstadt, Germany) and products separated on a DNA capillary sequencer (ABI 3100 Genetic Analyzer). .. Detection of nucleotide variants by restriction fragment length polymorphism analysis A 361 bp PCR product encompassing exon 8 of the WDR36 gene was digested with 1 U Hpy CH4III restriction enzyme (NEB, Beverly, MA).

    Purification:

    Article Title: Prevalence of resistance-associated substitutions to direct-acting antiviral agents in hemodialysis and renal transplant patients infected with hepatitis C virus
    Article Snippet: .. The nested PCR products were purified with 4 µL of ExoSAP-IT reagent (Affymetrix, Santa Clara, CA, USA) according to manufacturer instructions. .. A cycle-sequencing reaction was performed using the BigDye® Terminator v3.1 Kit (Thermo Fisher Scientific) and the same primers as employed in the nested PCR.

    Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma
    Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by ExoSAP-IT treatment (USB, Cleveland, OH), sequenced using Big Dye Termination chemistry (Applied Biosystems, Weiterstadt, Germany) and products separated on a DNA capillary sequencer (ABI 3100 Genetic Analyzer). .. Detection of nucleotide variants by restriction fragment length polymorphism analysis A 361 bp PCR product encompassing exon 8 of the WDR36 gene was digested with 1 U Hpy CH4III restriction enzyme (NEB, Beverly, MA).

    Article Title: Use of Phage Display to Identify Novel Mineralocorticoid Receptor-Interacting Proteins
    Article Snippet: .. PCR products were purified using ExoSap-IT according to the manufacturer's protocol (Affymetrix/USB; number 78200). .. Five microliters of the PCR product and 1 μL of ExoSap-IT was mixed and incubated at 37°C for 30 minutes.

    Nested PCR:

    Article Title: Prevalence of resistance-associated substitutions to direct-acting antiviral agents in hemodialysis and renal transplant patients infected with hepatitis C virus
    Article Snippet: .. The nested PCR products were purified with 4 µL of ExoSAP-IT reagent (Affymetrix, Santa Clara, CA, USA) according to manufacturer instructions. .. A cycle-sequencing reaction was performed using the BigDye® Terminator v3.1 Kit (Thermo Fisher Scientific) and the same primers as employed in the nested PCR.

    Incubation:

    Article Title: Association of FOXP3 Single Nucleotide Polymorphisms With Clinical Outcomes After Allogenic Hematopoietic Stem Cell Transplantation
    Article Snippet: .. Next, 2 µL ExoSAP-IT PCR Clean Up (Affymetrix, Santa Clara, CA, USA) was added to 5 µL of PCR product, followed by incubation at 37℃ for 15 minutes and at 80℃ for 15 minutes. .. We then added 1 µL of 5 pmol/µL sequencing primer, 4 µL of deionized water, and 4 µL of BigDye Terminator Ready Reaction Mix (Life Technologies, Grand Island, NY, USA) to 1 µL of purified PCR product.

    DNA Sequencing:

    Article Title: A New PCR-Based Method Shows That Blue Crabs (Callinectes sapidus (Rathbun)) Consume Winter Flounder (Pseudopleuronectes americanus (Walbaum))
    Article Snippet: .. DNA Sequencing Selected PCR products were treated with ExoSAP-It in accordance with the manufacturer’s protocol (Affymetrix, Santa Clara, CA, USA), combined with an appropriate primer and submitted to the Stony Brook University DNA Sequencing Facility. ..

    Sequencing:

    Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma
    Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by ExoSAP-IT treatment (USB, Cleveland, OH), sequenced using Big Dye Termination chemistry (Applied Biosystems, Weiterstadt, Germany) and products separated on a DNA capillary sequencer (ABI 3100 Genetic Analyzer). .. Detection of nucleotide variants by restriction fragment length polymorphism analysis A 361 bp PCR product encompassing exon 8 of the WDR36 gene was digested with 1 U Hpy CH4III restriction enzyme (NEB, Beverly, MA).

    Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S]
    Article Snippet: .. PCR products of positive clones were cleaned with ExoSAP-it (Affymetrix) and sequenced using BigDye Terminator v3.1 reagent (Applied Biosystems) at the sequencing facility of the University of Bergen. .. In situ hybridization To confirm the in situ hybridization specificity, two different single stranded digoxigenin (DIG)-labeled RNA probes of 476 bp and 604 bp lengths corresponding to different regions of LsMTP transcripts ( ) were synthesized separately from cDNA using the DIG RNA labeling kit (Roche).

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  • 99
    Thermo Fisher chargeswitch pcr clean up kit
    Chargeswitch Pcr Clean Up Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chargeswitch pcr clean up kit/product/Thermo Fisher
    Average 99 stars, based on 53 article reviews
    Price from $9.99 to $1999.99
    chargeswitch pcr clean up kit - by Bioz Stars, 2021-01
    99/100 stars
      Buy from Supplier

    92
    Thermo Fisher pcr clean up kit
    Essential role of PRAMEF12 in spermatogonial differentiation. a Immunofluorescence of whole-mount testes from P2, P4, and P7 Pramef12 Null and Pramef12 Het mice after staining with antibodies to DDX4 (left) or KIT (middle) and merged (right). KIT-positive spermatogonia were rarely detected at P2 in either Pramef12 Null or Pramef12 Het tubules but were present at P4 and increased at P7 in Pramef12 Het tubules. Few KIT-positive spermatogonia (arrow) were observed in P7 Pramef12 Null tubules. Scale bar, 50 μm. b Immunohistochemistry of sections from P7 Pramef12 Null (top panels) and Pramef12 Het (bottom panels) testes after staining with antibodies to DDX4 (left) or KIT (middle) and merged with Hoechst 33342 to stain DNA (right). Arrowheads, KIT-positive cells in Pramef12 Null testes. Scale bar, 50 μm. c Quantitative real-time <t>RT-PCR</t> of Kit <t>mRNA</t> abundance in P7 Pramef12 Null and Pramef12 Het testes using β-actin as an internal load control and setting the abundance in Pramef12 Het testis to 1. Mean ± s.d, n = 3 biologically independent samples per condition. * P = 1.43E-05 by two-tailed Student’s t test. d Immunoblot of KIT protein in P7 Pramef12 Null and Pramef12 Het testes using α-tubulin as a load control. e Same as a , but with antibodies to SOHLH1, SOHLH2, and PLZF. The number of spermatogonia positive for SOHLH1 and SOHLH2 was significantly reduced in P7 Pramef12 Null tubules (top panels). Representative of n = 3 a , b , d , e independent biological replicates with similar results per condition
    Pcr Clean Up Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/Thermo Fisher
    Average 92 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    88
    Thermo Fisher pcr clean up kit machery nagel
    Essential role of PRAMEF12 in spermatogonial differentiation. a Immunofluorescence of whole-mount testes from P2, P4, and P7 Pramef12 Null and Pramef12 Het mice after staining with antibodies to DDX4 (left) or KIT (middle) and merged (right). KIT-positive spermatogonia were rarely detected at P2 in either Pramef12 Null or Pramef12 Het tubules but were present at P4 and increased at P7 in Pramef12 Het tubules. Few KIT-positive spermatogonia (arrow) were observed in P7 Pramef12 Null tubules. Scale bar, 50 μm. b Immunohistochemistry of sections from P7 Pramef12 Null (top panels) and Pramef12 Het (bottom panels) testes after staining with antibodies to DDX4 (left) or KIT (middle) and merged with Hoechst 33342 to stain DNA (right). Arrowheads, KIT-positive cells in Pramef12 Null testes. Scale bar, 50 μm. c Quantitative real-time <t>RT-PCR</t> of Kit <t>mRNA</t> abundance in P7 Pramef12 Null and Pramef12 Het testes using β-actin as an internal load control and setting the abundance in Pramef12 Het testis to 1. Mean ± s.d, n = 3 biologically independent samples per condition. * P = 1.43E-05 by two-tailed Student’s t test. d Immunoblot of KIT protein in P7 Pramef12 Null and Pramef12 Het testes using α-tubulin as a load control. e Same as a , but with antibodies to SOHLH1, SOHLH2, and PLZF. The number of spermatogonia positive for SOHLH1 and SOHLH2 was significantly reduced in P7 Pramef12 Null tubules (top panels). Representative of n = 3 a , b , d , e independent biological replicates with similar results per condition
    Pcr Clean Up Kit Machery Nagel, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit machery nagel/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit machery nagel - by Bioz Stars, 2021-01
    88/100 stars
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    Image Search Results


    Essential role of PRAMEF12 in spermatogonial differentiation. a Immunofluorescence of whole-mount testes from P2, P4, and P7 Pramef12 Null and Pramef12 Het mice after staining with antibodies to DDX4 (left) or KIT (middle) and merged (right). KIT-positive spermatogonia were rarely detected at P2 in either Pramef12 Null or Pramef12 Het tubules but were present at P4 and increased at P7 in Pramef12 Het tubules. Few KIT-positive spermatogonia (arrow) were observed in P7 Pramef12 Null tubules. Scale bar, 50 μm. b Immunohistochemistry of sections from P7 Pramef12 Null (top panels) and Pramef12 Het (bottom panels) testes after staining with antibodies to DDX4 (left) or KIT (middle) and merged with Hoechst 33342 to stain DNA (right). Arrowheads, KIT-positive cells in Pramef12 Null testes. Scale bar, 50 μm. c Quantitative real-time RT-PCR of Kit mRNA abundance in P7 Pramef12 Null and Pramef12 Het testes using β-actin as an internal load control and setting the abundance in Pramef12 Het testis to 1. Mean ± s.d, n = 3 biologically independent samples per condition. * P = 1.43E-05 by two-tailed Student’s t test. d Immunoblot of KIT protein in P7 Pramef12 Null and Pramef12 Het testes using α-tubulin as a load control. e Same as a , but with antibodies to SOHLH1, SOHLH2, and PLZF. The number of spermatogonia positive for SOHLH1 and SOHLH2 was significantly reduced in P7 Pramef12 Null tubules (top panels). Representative of n = 3 a , b , d , e independent biological replicates with similar results per condition

    Journal: Nature Communications

    Article Title: Sertoli cell-only phenotype and scRNA-seq define PRAMEF12 as a factor essential for spermatogenesis in mice

    doi: 10.1038/s41467-019-13193-3

    Figure Lengend Snippet: Essential role of PRAMEF12 in spermatogonial differentiation. a Immunofluorescence of whole-mount testes from P2, P4, and P7 Pramef12 Null and Pramef12 Het mice after staining with antibodies to DDX4 (left) or KIT (middle) and merged (right). KIT-positive spermatogonia were rarely detected at P2 in either Pramef12 Null or Pramef12 Het tubules but were present at P4 and increased at P7 in Pramef12 Het tubules. Few KIT-positive spermatogonia (arrow) were observed in P7 Pramef12 Null tubules. Scale bar, 50 μm. b Immunohistochemistry of sections from P7 Pramef12 Null (top panels) and Pramef12 Het (bottom panels) testes after staining with antibodies to DDX4 (left) or KIT (middle) and merged with Hoechst 33342 to stain DNA (right). Arrowheads, KIT-positive cells in Pramef12 Null testes. Scale bar, 50 μm. c Quantitative real-time RT-PCR of Kit mRNA abundance in P7 Pramef12 Null and Pramef12 Het testes using β-actin as an internal load control and setting the abundance in Pramef12 Het testis to 1. Mean ± s.d, n = 3 biologically independent samples per condition. * P = 1.43E-05 by two-tailed Student’s t test. d Immunoblot of KIT protein in P7 Pramef12 Null and Pramef12 Het testes using α-tubulin as a load control. e Same as a , but with antibodies to SOHLH1, SOHLH2, and PLZF. The number of spermatogonia positive for SOHLH1 and SOHLH2 was significantly reduced in P7 Pramef12 Null tubules (top panels). Representative of n = 3 a , b , d , e independent biological replicates with similar results per condition

    Article Snippet: Cas9 mRNA (Addgene #42251) was generated by linearization with PmeI, purified with PCR clean-up kit, and transcribed with mMESSAGE mMACHINE T7 Kit (Thermo Fisher Scientific).

    Techniques: Immunofluorescence, Mouse Assay, Staining, Immunohistochemistry, Quantitative RT-PCR, Two Tailed Test