pcr clean up (Thermo Fisher)
Thermo Fisher is a verified supplier 
99
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Name:
ExoSAP IT Express PCR Product Cleanup Reagent
Description:
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
Catalog Number:
75001.1.ea
Price:
None
Applications:
PCR|PCR & Real-Time PCR|Sequencing
Category:
Proteins Enzymes Peptides
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Structured Review
Thermo Fisher
pcr clean up
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
https://www.bioz.com/result/pcr clean up/product/Thermo Fisher
Average 99 stars, based on 31 article reviews
Price from $9.99 to $1999.99
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
https://www.bioz.com/result/pcr clean up/product/Thermo Fisher
Average 99 stars, based on 31 article reviews
Price from $9.99 to $1999.99
pcr clean up - by Bioz Stars,
2021-01
99/100 stars
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Clone Assay:Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S] Article Snippet: .. PCR products of positive clones were cleaned with Polymerase Chain Reaction:Article Title: Association of FOXP3 Single Nucleotide Polymorphisms With Clinical Outcomes After Allogenic Hematopoietic Stem Cell Transplantation Article Snippet: .. Next, 2 µL Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S] Article Snippet: .. PCR products of positive clones were cleaned with Article Title: Use of Phage Display to Identify Novel Mineralocorticoid Receptor-Interacting Proteins Article Snippet: .. PCR products were purified using Article Title: A New PCR-Based Method Shows That Blue Crabs (Callinectes sapidus (Rathbun)) Consume Winter Flounder (Pseudopleuronectes americanus (Walbaum)) Article Snippet: .. DNA Sequencing Selected PCR products were treated with Mutagenesis:Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by Purification:Article Title: Prevalence of resistance-associated substitutions to direct-acting antiviral agents in hemodialysis and renal transplant patients infected with hepatitis C virus Article Snippet: .. The nested PCR products were purified with 4 µL of Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by Article Title: Use of Phage Display to Identify Novel Mineralocorticoid Receptor-Interacting Proteins Article Snippet: .. PCR products were purified using Nested PCR:Article Title: Prevalence of resistance-associated substitutions to direct-acting antiviral agents in hemodialysis and renal transplant patients infected with hepatitis C virus Article Snippet: .. The nested PCR products were purified with 4 µL of Incubation:Article Title: Association of FOXP3 Single Nucleotide Polymorphisms With Clinical Outcomes After Allogenic Hematopoietic Stem Cell Transplantation Article Snippet: .. Next, 2 µL DNA Sequencing:Article Title: A New PCR-Based Method Shows That Blue Crabs (Callinectes sapidus (Rathbun)) Consume Winter Flounder (Pseudopleuronectes americanus (Walbaum)) Article Snippet: .. DNA Sequencing Selected PCR products were treated with Sequencing:Article Title: Variations in the WDR36 gene in German patients with normal tension glaucoma Article Snippet: .. Mutation detection by direct sequencing PCR fragments were purified by Article Title: Microsomal triglyceride transfer protein in the ectoparasitic crustacean salmon louse (Lepeophtheirus salmonis) [S] Article Snippet: .. PCR products of positive clones were cleaned with |
