pcr clean up (Thermo Fisher)
Thermo Fisher is a verified supplier 
99
|
Name:
ExoSAP IT Express PCR Product Cleanup Reagent
Description:
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
Catalog Number:
75001.1.ea
Price:
None
Applications:
PCR|PCR & Real-Time PCR|Sequencing
Category:
Proteins Enzymes Peptides
|
Buy from Supplier |
Structured Review
Thermo Fisher
pcr clean up
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
https://www.bioz.com/result/pcr clean up/product/Thermo Fisher
Average 99 stars, based on 31 article reviews
Price from $9.99 to $1999.99
In only 5 minutes ExoSAP IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction This enzymatic cleanup method offers precision increased yield and a faster turnaround time compared to alternative purification methods such as spin columns or beads • Remove unincorporated primers and nucleotides in about 5 minutes • Prepare DNA sequencing samples with one pipetting step • Conserve precious PCR products with up to 100 recovery regardless of amplicon length • Minimize spin column or bead cleanup ExoSAP IT Express PCR Product Cleanup Reagent consists of two hydrolytic enzymes a novel exonuclease I and shrimp alkaline phosphatase SAP in a specially formulated buffer The reaction setup is complete with one pipetting step which is followed by two short incubations The first incubation hydrolyzes excess primer and dephosphorylates nucleotides The second high temperature incubation completely and irreversibly inactivates the enzymes in about one minute Adding ExoSAP IT Express reagent directly to the PCR product eliminates transfer steps to tubes wells or columns conserves PCR amplicons helping reduce the chance of cross contamination no further processing is needed Samples are ready for DNA sequencing or SNP analysis
https://www.bioz.com/result/pcr clean up/product/Thermo Fisher
Average 99 stars, based on 31 article reviews
Price from $9.99 to $1999.99
pcr clean up - by Bioz Stars,
2020-08
99/100 stars
Images
Related Articles
Amplification:Article Title: Molecular evidence supports the expansion of visceral leishmaniasis towards non-program districts of Nepal Article Snippet: .. Amplicon purification and sequencing The PCR product was purified using Agarose Gel Electrophoresis:Article Title: GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors Article Snippet: .. The PCR product was separated on an agarose gel by electrophoresis, extracted using the QIAquick Gel Extraction Kit (Qiagen), re-amplified using the same primers, and purified using Purification:Article Title: Disrupted auto-regulation of the spliceosomal gene SNRPB causes cerebro–costo–mandibular syndrome Article Snippet: .. A quantity of 1.2 μl of 1/20 dilution of the PCR product was purified in a reaction with 1 μl Article Title: Molecular evidence supports the expansion of visceral leishmaniasis towards non-program districts of Nepal Article Snippet: .. Amplicon purification and sequencing The PCR product was purified using Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR Article Snippet: .. BNA-clamp PCR products were purified using Article Title: GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors Article Snippet: .. The PCR product was separated on an agarose gel by electrophoresis, extracted using the QIAquick Gel Extraction Kit (Qiagen), re-amplified using the same primers, and purified using Electrophoresis:Article Title: GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors Article Snippet: .. The PCR product was separated on an agarose gel by electrophoresis, extracted using the QIAquick Gel Extraction Kit (Qiagen), re-amplified using the same primers, and purified using Polymerase Chain Reaction:Article Title: Accurate detection of de novo and transmitted indels within exome-capture data using micro-assembly Article Snippet: .. PCR product was verified on E-Gel® 96 gels (Invitrogen Corp., Carlsbad, CA, USA) and subsequently pooled for Article Title: Disrupted auto-regulation of the spliceosomal gene SNRPB causes cerebro–costo–mandibular syndrome Article Snippet: .. A quantity of 1.2 μl of 1/20 dilution of the PCR product was purified in a reaction with 1 μl Article Title: Extended-Spectrum Beta-Lactamase Gene Sequences in Gram-Negative Saprophytes on Retail Organic and Nonorganic Spinach ▿ Article Snippet: .. Each PCR product that showed an electrophoretic band of an expected size was cleaned up by using Article Title: Molecular evidence supports the expansion of visceral leishmaniasis towards non-program districts of Nepal Article Snippet: .. Amplicon purification and sequencing The PCR product was purified using Article Title: Accurate detection of KRAS, NRAS and BRAF mutations in metastatic colorectal cancers by bridged nucleic acid-clamp real-time PCR Article Snippet: .. BNA-clamp PCR products were purified using Article Title: C3orf70 Is Involved in Neural and Neurobehavioral Development Article Snippet: .. The PCR product corresponding to the homo-KO of c3orf70a or c3orf70b was subjected to sequence analysis using Article Title: New susceptibility locus for obesity and dyslipidaemia on chromosome 3q22.3 Article Snippet: .. Five microlitres of PCR product was then treated with 2 μl of Article Title: GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors Article Snippet: .. The PCR product was separated on an agarose gel by electrophoresis, extracted using the QIAquick Gel Extraction Kit (Qiagen), re-amplified using the same primers, and purified using Sequencing:Article Title: Disrupted auto-regulation of the spliceosomal gene SNRPB causes cerebro–costo–mandibular syndrome Article Snippet: .. A quantity of 1.2 μl of 1/20 dilution of the PCR product was purified in a reaction with 1 μl Article Title: Molecular evidence supports the expansion of visceral leishmaniasis towards non-program districts of Nepal Article Snippet: .. Amplicon purification and sequencing The PCR product was purified using Article Title: C3orf70 Is Involved in Neural and Neurobehavioral Development Article Snippet: .. The PCR product corresponding to the homo-KO of c3orf70a or c3orf70b was subjected to sequence analysis using Gel Extraction:Article Title: GATA4 mediates gene repression in the mature mouse small intestine through interactions with Friend of GATA (FOG) cofactors Article Snippet: .. The PCR product was separated on an agarose gel by electrophoresis, extracted using the QIAquick Gel Extraction Kit (Qiagen), re-amplified using the same primers, and purified using |
