pcr clean up (TaKaRa)
Name:
NucleoSpin Gel and PCR Clean Up
Description:
With the NucleoSpin Gel and PCR Clean Up kits you get two applications in one The supplied columns which are suitable for gel extraction as well as PCR purification bind DNA to a silica membrane in the presence of a chaotropic salt Once the binding mixture is loaded onto the column contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3 providing rapid DNA purification
Catalog Number:
740609.50
Price:
None
Size:
50 Preps
Category:
NucleoSpin Gel and PCR Clean Up DNA cleanup kits Nucleic acid purification
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Structured Review

With the NucleoSpin Gel and PCR Clean Up kits you get two applications in one The supplied columns which are suitable for gel extraction as well as PCR purification bind DNA to a silica membrane in the presence of a chaotropic salt Once the binding mixture is loaded onto the column contaminants are removed by simple wash steps with ethanolic Wash Buffer NT3 providing rapid DNA purification
https://www.bioz.com/result/pcr clean up/product/TaKaRa
Average 99 stars, based on 38 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors"
Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors
Journal: Nature protocols
doi: 10.1038/nprot.2017.153

Figure Legend Snippet: Schematic of iv TRT and ssDNA preparation steps. A dsDNA template can be a PCR product, or a plasmid with a suitable restriction enzyme (RE) site distal to the insertion cassette (Steps 1–2) . The gel on the right shows a plasmid digested with a RE. RNA is synthesized using in vitro transcription (Steps 3–18) . The gel on the right shows an RNA of ~ 900 bases long. cDNA is synthesized by reverse transcription using a reverse primer (Steps 19–23) . The gel on the right shows a sample ssDNA (cDNA). Note that the cDNA preparation typically runs like a smear with a prominent band within the smear. Purification of ssDNA (Steps 24–35) . The image on the left shows the gel after excision of the prominent band (for purification) and the gel on the right shows the purified ssDNA. The GeneRuler DNA Ladder Mix (ThermoFisher Scientific, cat. no. SM0331) was used in all the gels as a DNA size marker.
Techniques Used: Polymerase Chain Reaction, Plasmid Preparation, Synthesized, In Vitro, Purification, Marker
2) Product Images from "CLICK: one-step generation of conditional knockout mice"
Article Title: CLICK: one-step generation of conditional knockout mice
Journal: BMC Genomics
doi: 10.1186/s12864-018-4713-y

Figure Legend Snippet: Rat floxed alleles generated by zygote electroporation. a Schematic representation of CLICK: CRISPR with lssDNA inducing conditional knockout alleles. b Schematic approach to generate rat Vapb floxed alleles using the CRISPR/Cas system with lssDNA composed of the targeted exon flanked by two loxP sites (Additional file 11 : Figure S11). c PCR analysis of rat pups (#1–6) generated by CLICK, showing different types of mutations, indels, LD (via NHEJ), inversions, and floxed alleles (via HDR) (black arrow). The primer sets were used for PCR and sequence analysis (Additional file 2 : Figure S2). M: 100 bp DNA ladder marker. d Sequence analysis of 6 pups showing a variety of mutations, indels, inversion or LD indicated by red letters, and loxP insertions and flox indicated by orange letters. All pups were used for testing germline transmission (Additional file 15 : Table S1). Asterisks indicate pups used for testing the Cre-loxP system (Additional file 17 : Table S3)
Techniques Used: Generated, Electroporation, CRISPR, Knock-Out, Polymerase Chain Reaction, Non-Homologous End Joining, Sequencing, Marker, Transmission Assay

Figure Legend Snippet: One-step generation of conditional knockout animals (F0) by CLICK. a Schematic representation of applying CLICK in oocytes for in vitro fertilization (IVF) with Cre-driver mice, Emx1-cre, resulting in brain-specific recombination at the targeted floxed alleles. b PCR analysis of representative delivered mouse pups by microinjection (#1–8) or electroporation (#9–15) showing different types of mutations, indels, LD, and floxed alleles (black arrow) at the targeted Serpina3n locus. The primer sets (‘small’ in Additional file 2 : Figure S2) were used for PCR and sequence analysis. c Genotyping in several tissues: cerebrum (Cr), cerebellum (Cl), heart (H), liver (L), spleen (S), and testis (Ts), indicating recombination (red arrow) by brain-specific Cre expression in #14 mouse carrying heterozygous floxed with LD alleles. M: 100 bp DNA ladder marker. d Time-schedule comparisons of targeting methods using ES cells by CRISPR in B6 oocytes, and CLICK in Cre oocytes. CLICK saves about 6 months of crossing and reduces breeding costs during the study period (Additional file 13 : Figure S13)
Techniques Used: Knock-Out, In Vitro, Mouse Assay, Polymerase Chain Reaction, Electroporation, Sequencing, Expressing, Marker, CRISPR

Figure Legend Snippet: Mouse floxed alleles generated by microinjection of two gRNA, Cas9 mRNA and lssDNA into zygotes. a An approach to generate Serpina3n floxed alleles using the CRISPR/Cas system with long single-stranded DNA (lssDNA) composed of the targeted exon flanked by two loxP sites (Additional file 2 : Figure S2). b PCR analysis of representative delivered mouse pups (#1–15) showing different types of mutations, indels, LD, and floxed alleles (black arrow) at the targeted Serpina3n locus. The primer sets (‘small’ in Additional file 2 : Figure S2) were used for PCR and sequence analysis. Asterisks indicate pups used for testing germline transmission (Additional file 15 : Table S1). M: 100 bp DNA ladder marker. c Representative examples of the targeted Serpina3n loci generated by the microinjection of two gRNA (gRNA-1 and gRNA-2), Cas9 mRNA and lssDNA into B6 mouse zygotes (Additional file 3 : Figure S3)
Techniques Used: Generated, CRISPR, Polymerase Chain Reaction, Sequencing, Transmission Assay, Marker
3) Product Images from "Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors"
Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors
Journal: Nature protocols
doi: 10.1038/nprot.2017.153

Figure Legend Snippet: Schematic of iv TRT and ssDNA preparation steps. A dsDNA template can be a PCR product, or a plasmid with a suitable restriction enzyme (RE) site distal to the insertion cassette (Steps 1–2) . The gel on the right shows a plasmid digested with a RE. RNA is synthesized using in vitro transcription (Steps 3–18) . The gel on the right shows an RNA of ~ 900 bases long. cDNA is synthesized by reverse transcription using a reverse primer (Steps 19–23) . The gel on the right shows a sample ssDNA (cDNA). Note that the cDNA preparation typically runs like a smear with a prominent band within the smear. Purification of ssDNA (Steps 24–35) . The image on the left shows the gel after excision of the prominent band (for purification) and the gel on the right shows the purified ssDNA. The GeneRuler DNA Ladder Mix (ThermoFisher Scientific, cat. no. SM0331) was used in all the gels as a DNA size marker.
Techniques Used: Polymerase Chain Reaction, Plasmid Preparation, Synthesized, In Vitro, Purification, Marker
Related Articles
Polymerase Chain Reaction:Article Title: CLICK: one-step generation of conditional knockout mice Article Snippet: .. Bands corresponding to a single-strand DNA fragment were extracted using NucleoSpin® Gel and Article Title: Expression Patterns of PACAP and PAC1R Genes and Anorexigenic Action of PACAP1 and PACAP2 in Zebrafish Article Snippet: .. The PCR step comprised 98°C for the first 2 min, followed by 40 cycles of 98°C for 10 s, 58°C for 30 s, and 68°C for 1 min. After agarose electrophoresis, target bands were cut out and DNA was isolated using Article Title: CLICK: one-step generation of conditional knockout mice Article Snippet: .. Bands corresponding to a single-strand DNA fragment were extracted using NucleoSpin® Gel and Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. 28) Extract DNA from the gel slice using NucleoSpin Gel or Article Title: Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters Article Snippet: .. 10 μL) were purified using Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and Article Title: Multiple Skin Cancers in a Renal Transplant Recipient: A Patient Report with Analyses of Human Papillomavirus and Human Polyomavirus Infection Article Snippet: .. The PCR products were purified before sequencing using Article Title: Comparative Analysis of Gammaherpesvirus Circular RNA Repertoires: Conserved and Unique Viral Circular RNAs Article Snippet: .. PCR products were cut out and purified using the Ethanol Precipitation:Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. 28) Extract DNA from the gel slice using NucleoSpin Gel or Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and Purification:Article Title: Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High-Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters Article Snippet: .. 10 μL) were purified using Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and Article Title: Multiple Skin Cancers in a Renal Transplant Recipient: A Patient Report with Analyses of Human Papillomavirus and Human Polyomavirus Infection Article Snippet: .. The PCR products were purified before sequencing using Article Title: Comparative Analysis of Gammaherpesvirus Circular RNA Repertoires: Conserved and Unique Viral Circular RNAs Article Snippet: .. PCR products were cut out and purified using the Electrophoresis:Article Title: Expression Patterns of PACAP and PAC1R Genes and Anorexigenic Action of PACAP1 and PACAP2 in Zebrafish Article Snippet: .. The PCR step comprised 98°C for the first 2 min, followed by 40 cycles of 98°C for 10 s, 58°C for 30 s, and 68°C for 1 min. After agarose electrophoresis, target bands were cut out and DNA was isolated using Transgenic Assay:Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and Isolation:Article Title: Expression Patterns of PACAP and PAC1R Genes and Anorexigenic Action of PACAP1 and PACAP2 in Zebrafish Article Snippet: .. The PCR step comprised 98°C for the first 2 min, followed by 40 cycles of 98°C for 10 s, 58°C for 30 s, and 68°C for 1 min. After agarose electrophoresis, target bands were cut out and DNA was isolated using Sequencing:Article Title: Multiple Skin Cancers in a Renal Transplant Recipient: A Patient Report with Analyses of Human Papillomavirus and Human Polyomavirus Infection Article Snippet: .. The PCR products were purified before sequencing using Injection:Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and Gel Purification:Article Title: Easi-CRISPR protocol for creating knock-in and conditional knockout mouse models using long ssDNA donors Article Snippet: .. Steps 1–2, Preparation of dsDNA template: 5h Steps 3–5, RNA synthesis using T7 RiboMax Express: 1–4h Steps 6–18, Purification of RNA using MEGAclear kit: 1h Steps 19–23, Synthesis of cDNA from RNA: 1.5h Steps 24–28, Ethanol precipitation of cDNA and gel purification: 1.5h Step 28A, Using NucleoSpin Gel and |