pcr clean up  (MACHEREY NAGEL)

 
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    Name:
    NucleoSpin Gel and PCR Clean‑up Columns for gel extraction and PCR clean up
    Description:
    PCR clean up and gel extraction the two in one kit
    Catalog Number:
    740609.50s
    Price:
    None
    Size:
    50 Pieces
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    Bioanalysis Kits
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    Structured Review

    MACHEREY NAGEL pcr clean up
    NucleoSpin Gel and PCR Clean‑up Columns for gel extraction and PCR clean up
    PCR clean up and gel extraction the two in one kit
    https://www.bioz.com/result/pcr clean up/product/MACHEREY NAGEL
    Average 99 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Development of a DNA-Based Detection Method for Cocos Nucifera Using TaqMan™ Real-Time PCR"

    Article Title: Development of a DNA-Based Detection Method for Cocos Nucifera Using TaqMan™ Real-Time PCR

    Journal: Foods

    doi: 10.3390/foods9030332

    Gel electrophoresis of DNA extracted from different palm species to test the specificity of different sets of primer. Lane 1: Chilean wine palm ( Jubaea chilensis ); lane 2: oil palm ( Elaeis guineensis ); lane 3: coconut palm ( Cocos nucifera ), lane 4: jelly palm ( Butia capitata ); lane 5: non-template sample with water; lane 6: 100 bp DNA ladder. ( a ) PCR amplification with the primer set cocosPRK. ( b ) PCR amplification using the primer set cocosITS109. ( c ) PCR amplification performed with the primer set cocosITS197.
    Figure Legend Snippet: Gel electrophoresis of DNA extracted from different palm species to test the specificity of different sets of primer. Lane 1: Chilean wine palm ( Jubaea chilensis ); lane 2: oil palm ( Elaeis guineensis ); lane 3: coconut palm ( Cocos nucifera ), lane 4: jelly palm ( Butia capitata ); lane 5: non-template sample with water; lane 6: 100 bp DNA ladder. ( a ) PCR amplification with the primer set cocosPRK. ( b ) PCR amplification using the primer set cocosITS109. ( c ) PCR amplification performed with the primer set cocosITS197.

    Techniques Used: Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Amplification

    Sensitivity test of the real-time PCR assay with 10-fold diluted DNA of coconut in a concentration range from 100 ng/µL to 0.1 pg/µL. The reactions were performed in duplicates. ( a ) The amplification plot shows C t values for samples from 100 ng/µL to 1 pg/µL. ( b ) Standard curve of the coconut TagMan real-time PCR system by plotting C t values against the log 10 input DNA concentration.
    Figure Legend Snippet: Sensitivity test of the real-time PCR assay with 10-fold diluted DNA of coconut in a concentration range from 100 ng/µL to 0.1 pg/µL. The reactions were performed in duplicates. ( a ) The amplification plot shows C t values for samples from 100 ng/µL to 1 pg/µL. ( b ) Standard curve of the coconut TagMan real-time PCR system by plotting C t values against the log 10 input DNA concentration.

    Techniques Used: Real-time Polymerase Chain Reaction, Concentration Assay, Amplification

    2) Product Images from "Color Intensity of the Red-Fleshed Berry Phenotype of Vitis vinifera Teinturier Grapes Varies Due to a 408 bp Duplication in the Promoter of VvmybA1"

    Article Title: Color Intensity of the Red-Fleshed Berry Phenotype of Vitis vinifera Teinturier Grapes Varies Due to a 408 bp Duplication in the Promoter of VvmybA1

    Journal: Genes

    doi: 10.3390/genes11080891

    The tandem repeat mutation in the promoter region of VvmybA1 specific for teinturier varieties. ( A ) PCR result of the white ( MybA1a ), red ( MybA1c ) and teinturier alleles ( MybA1t2 , MybA1t3 and MybA1t5 ) of DNA prepared from leaves (L1 + L2) and roots (L2) of three different ‘Teinturier’ clones. ‘Pinot Noir’ as reference. ( B ) Schematic diagram of the three teinturier alleles found ( MybA1t2 , MybA1t3 and MybA1t5 ) named based on the repeat number of the 408 bp GCE element (grapevine color enhancer) in the promoter region of VvmybA1. Red and white alleles as reference.
    Figure Legend Snippet: The tandem repeat mutation in the promoter region of VvmybA1 specific for teinturier varieties. ( A ) PCR result of the white ( MybA1a ), red ( MybA1c ) and teinturier alleles ( MybA1t2 , MybA1t3 and MybA1t5 ) of DNA prepared from leaves (L1 + L2) and roots (L2) of three different ‘Teinturier’ clones. ‘Pinot Noir’ as reference. ( B ) Schematic diagram of the three teinturier alleles found ( MybA1t2 , MybA1t3 and MybA1t5 ) named based on the repeat number of the 408 bp GCE element (grapevine color enhancer) in the promoter region of VvmybA1. Red and white alleles as reference.

    Techniques Used: Mutagenesis, Polymerase Chain Reaction, Clone Assay

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Heterologous expression of plant glycosyltransferases for biochemistry and structural biology
    Article Snippet: .. Gel purify the completed attB-PCR products using the NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel) and clone into pDONR221 (ThermoFisher Scientific) using Gateway BP Clonase II Enzyme Mix (ThermoFisher Scientific) according to the manufacturer’s instructions. .. Screen resultant colonies to identify positive transformants by colony PCR and confirm by sequence analysis using M13 universal primers: M13 (−20) Forward and M13 Reverse (ThermoFisher Scientific).

    Article Title: The fenestrae-associated protein Plvap regulates the rate of blood-borne protein passage into the hypophysis
    Article Snippet: .. To genotype for the plvapbsa13080 allele, the amplified DNA region was detected by 0.8-1% agarose gel electrophoresis, then extracted and purified by using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). .. Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science.

    Article Title: Hippocampal Stimulation Promotes Intracellular Tip60 Dynamics with Concomitant Genome Reorganization and Synaptic Gene Activation.
    Article Snippet: .. Resulting double-stranded PCR products were purified using NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). .. Purified double-stranded DNA was then used as the template for the generation of single-stranded DNA FISH probes via asymmetrical PCR with the incorporation of modified nucleotides.

    Amplification:

    Article Title: The fenestrae-associated protein Plvap regulates the rate of blood-borne protein passage into the hypophysis
    Article Snippet: .. To genotype for the plvapbsa13080 allele, the amplified DNA region was detected by 0.8-1% agarose gel electrophoresis, then extracted and purified by using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). .. Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science.

    Agarose Gel Electrophoresis:

    Article Title: The fenestrae-associated protein Plvap regulates the rate of blood-borne protein passage into the hypophysis
    Article Snippet: .. To genotype for the plvapbsa13080 allele, the amplified DNA region was detected by 0.8-1% agarose gel electrophoresis, then extracted and purified by using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). .. Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science.

    other:

    Article Title: Three New Reports of Trichoderma in Algeria: T. atrobrunneum, (South) T. longibrachiatum (South), and T. afroharzianum (Northwest)
    Article Snippet: The PCR products were detected using 1% agarose gel electrophoresis and purified according to the gel extraction (NucleoSpin Extract II, Macherey-Nagel, Düren, Germany) clean-up kit protocol.

    Purification:

    Article Title: The fenestrae-associated protein Plvap regulates the rate of blood-borne protein passage into the hypophysis
    Article Snippet: .. To genotype for the plvapbsa13080 allele, the amplified DNA region was detected by 0.8-1% agarose gel electrophoresis, then extracted and purified by using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). .. Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science.

    Article Title: Hippocampal Stimulation Promotes Intracellular Tip60 Dynamics with Concomitant Genome Reorganization and Synaptic Gene Activation.
    Article Snippet: .. Resulting double-stranded PCR products were purified using NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). .. Purified double-stranded DNA was then used as the template for the generation of single-stranded DNA FISH probes via asymmetrical PCR with the incorporation of modified nucleotides.

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  • 92
    MACHEREY NAGEL pcr clean up kit
    Host and viral <t>DNA</t> content of differently treated ASFV samples. Quantitative dual <t>PCR</t> was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.
    Pcr Clean Up Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/MACHEREY NAGEL
    Average 92 stars, based on 844 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    85
    MACHEREY NAGEL pcr clean up spin columns
    Real-time <t>PCR</t> analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target <t>amplicon,</t> the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
    Pcr Clean Up Spin Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up spin columns/product/MACHEREY NAGEL
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr clean up spin columns - by Bioz Stars, 2021-01
    85/100 stars
      Buy from Supplier

    92
    MACHEREY NAGEL pcr clean up
    Identification of heterozygous fish carrying the expected nucleotide substitution at the target site. ( A ) Schematic representation of allele-specific <t>PCR</t> analysis of the <t>chd</t> gene. ( B ) An example of the results after allele-specific PCR amplification of the chd gene. The 203 bp DNA fragments in the”w” lanes were amplified by the primer set of chd _wF and chd _wR primers, whereas fragments in the “m” lanes were amplified by the primer set chd _mF and chd _wR. The 635 bp DNA fragments were amplified by a primer set recognizing a different region of the chd gene as an internal control. ( C ) Results of analyzing all individuals by allele-specific PCR for the chd gene. “Negative” indicates the absence of a nucleotide substitution at the target site, while “positive” indicates the presence of a nucleotide substitution at the target site. ( D ) Results of analyzing 12 positive individuals by sequencing the targeted region of the chd gene. ( E ) Schematic representation of allele-specific PCR analysis for the oep gene. ( F ) An example of results after allele-specific PCR amplification of the oep gene. The 183 bp DNA fragments in the”w” lanes were amplified by the primer set oep _wF and oep _wR, whereas fragments in the “m” lanes were amplified by the primer set oep _wF and oep _mR. The 415 bp DNA fragments were amplified by a primer set recognizing a different region of the oep gene as an internal control. ( G ) Results of analyzing all individuals by allele-specific PCR for the oep gene. ( H ) Results of analyzing 16 positive individuals by sequencing the targeted region of the oep gene. ( D , H ) The red arrowheads indicate the nucleotide substitution at the target site. ( H ) The black arrowhead indicates the nucleotide substitution at the untargeted site.
    Pcr Clean Up, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up/product/MACHEREY NAGEL
    Average 92 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    89
    MACHEREY NAGEL pcr clean up columns
    Characterization of a stable <t>eys</t> rmc101/rmc101 zebrafish line. (A) Sanger sequencing identified a five base pair deletion in exon 20 in eys rmc101/rmc101 zebrafish. (B) Representative gel image of <t>RT-PCR</t> analysis using RNA from a pool of larvae (n = 15), which shows that eys transcripts are present in both wild-type and eys rmc101/rmc101 zebrafish (upper panel). Sanger sequencing confirmed the presence of the five base pair deletion in the eys rmc101/rmc101 transcript (lower panel). (C) Protein domain structures of wild-type Eys and the truncated Eys protein that is predicted in eys rmc101/rmc101 zebrafish.
    Pcr Clean Up Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 89/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up columns/product/MACHEREY NAGEL
    Average 89 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    pcr clean up columns - by Bioz Stars, 2021-01
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    Image Search Results


    Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Journal: Viruses

    Article Title: A Simple Method for Sample Preparation to Facilitate Efficient Whole-Genome Sequencing of African Swine Fever Virus

    doi: 10.3390/v11121129

    Figure Lengend Snippet: Host and viral DNA content of differently treated ASFV samples. Quantitative dual PCR was executed by Virotype ASFV PCR Kit. C t values of individual and averaged samples represented by colored circles and grey box respectively. Averages were calculated from numbers of samples indicated on X axis. Gray circles indicate minimum and maximum values. C t values higher than 40 (undetectable host DNA) are represented by 41. Whole genome amplification (WGA) was executed by REPLI-g Mini Kit.

    Article Snippet: Amplified DNA Clean Up REPLI-g samples were purified using the NucleoSpin Gel and PCR clean-up Kit (Macherey-Nagel Düren, Germany).

    Techniques: Polymerase Chain Reaction, Whole Genome Amplification

    Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.

    Journal: Nucleic Acids Research

    Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases

    doi: 10.1093/nar/gkx116

    Figure Lengend Snippet: Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.

    Article Snippet: PCR amplicon DNA was isolated from gel slices using NuceloSpin Gel and PCR Clean-up spin columns, eluting in 30 μl volume (Macherey-Nagel).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Amplification

    Identification of heterozygous fish carrying the expected nucleotide substitution at the target site. ( A ) Schematic representation of allele-specific PCR analysis of the chd gene. ( B ) An example of the results after allele-specific PCR amplification of the chd gene. The 203 bp DNA fragments in the”w” lanes were amplified by the primer set of chd _wF and chd _wR primers, whereas fragments in the “m” lanes were amplified by the primer set chd _mF and chd _wR. The 635 bp DNA fragments were amplified by a primer set recognizing a different region of the chd gene as an internal control. ( C ) Results of analyzing all individuals by allele-specific PCR for the chd gene. “Negative” indicates the absence of a nucleotide substitution at the target site, while “positive” indicates the presence of a nucleotide substitution at the target site. ( D ) Results of analyzing 12 positive individuals by sequencing the targeted region of the chd gene. ( E ) Schematic representation of allele-specific PCR analysis for the oep gene. ( F ) An example of results after allele-specific PCR amplification of the oep gene. The 183 bp DNA fragments in the”w” lanes were amplified by the primer set oep _wF and oep _wR, whereas fragments in the “m” lanes were amplified by the primer set oep _wF and oep _mR. The 415 bp DNA fragments were amplified by a primer set recognizing a different region of the oep gene as an internal control. ( G ) Results of analyzing all individuals by allele-specific PCR for the oep gene. ( H ) Results of analyzing 16 positive individuals by sequencing the targeted region of the oep gene. ( D , H ) The red arrowheads indicate the nucleotide substitution at the target site. ( H ) The black arrowhead indicates the nucleotide substitution at the untargeted site.

    Journal: Scientific Reports

    Article Title: In vivo targeted single-nucleotide editing in zebrafish

    doi: 10.1038/s41598-018-29794-9

    Figure Lengend Snippet: Identification of heterozygous fish carrying the expected nucleotide substitution at the target site. ( A ) Schematic representation of allele-specific PCR analysis of the chd gene. ( B ) An example of the results after allele-specific PCR amplification of the chd gene. The 203 bp DNA fragments in the”w” lanes were amplified by the primer set of chd _wF and chd _wR primers, whereas fragments in the “m” lanes were amplified by the primer set chd _mF and chd _wR. The 635 bp DNA fragments were amplified by a primer set recognizing a different region of the chd gene as an internal control. ( C ) Results of analyzing all individuals by allele-specific PCR for the chd gene. “Negative” indicates the absence of a nucleotide substitution at the target site, while “positive” indicates the presence of a nucleotide substitution at the target site. ( D ) Results of analyzing 12 positive individuals by sequencing the targeted region of the chd gene. ( E ) Schematic representation of allele-specific PCR analysis for the oep gene. ( F ) An example of results after allele-specific PCR amplification of the oep gene. The 183 bp DNA fragments in the”w” lanes were amplified by the primer set oep _wF and oep _wR, whereas fragments in the “m” lanes were amplified by the primer set oep _wF and oep _mR. The 415 bp DNA fragments were amplified by a primer set recognizing a different region of the oep gene as an internal control. ( G ) Results of analyzing all individuals by allele-specific PCR for the oep gene. ( H ) Results of analyzing 16 positive individuals by sequencing the targeted region of the oep gene. ( D , H ) The red arrowheads indicate the nucleotide substitution at the target site. ( H ) The black arrowhead indicates the nucleotide substitution at the untargeted site.

    Article Snippet: The PCR products from the chd and oep amplifications were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany) and sequenced by Fasmac (Atsugi, Japan) or by Macrogen Japan (Kyoto, Japan) with the chd _1st_F and oep _1st_F primers, respectively.

    Techniques: Fluorescence In Situ Hybridization, Polymerase Chain Reaction, Amplification, Sequencing

    Characterization of a stable eys rmc101/rmc101 zebrafish line. (A) Sanger sequencing identified a five base pair deletion in exon 20 in eys rmc101/rmc101 zebrafish. (B) Representative gel image of RT-PCR analysis using RNA from a pool of larvae (n = 15), which shows that eys transcripts are present in both wild-type and eys rmc101/rmc101 zebrafish (upper panel). Sanger sequencing confirmed the presence of the five base pair deletion in the eys rmc101/rmc101 transcript (lower panel). (C) Protein domain structures of wild-type Eys and the truncated Eys protein that is predicted in eys rmc101/rmc101 zebrafish.

    Journal: PLoS ONE

    Article Title: Eyes shut homolog is important for the maintenance of photoreceptor morphology and visual function in zebrafish

    doi: 10.1371/journal.pone.0200789

    Figure Lengend Snippet: Characterization of a stable eys rmc101/rmc101 zebrafish line. (A) Sanger sequencing identified a five base pair deletion in exon 20 in eys rmc101/rmc101 zebrafish. (B) Representative gel image of RT-PCR analysis using RNA from a pool of larvae (n = 15), which shows that eys transcripts are present in both wild-type and eys rmc101/rmc101 zebrafish (upper panel). Sanger sequencing confirmed the presence of the five base pair deletion in the eys rmc101/rmc101 transcript (lower panel). (C) Protein domain structures of wild-type Eys and the truncated Eys protein that is predicted in eys rmc101/rmc101 zebrafish.

    Article Snippet: To verify that the amplified products indeed correspond to eys , PCR products were purified on Nucleospin Gel and PCR Clean-up columns (Macherey Nagel) and either directly sequenced or cloned in the pCR4-TOPO vector with the use of the TOPO TA Cloning Kit (Invitrogen) for sequencing with T7 and T3 sequencing primers.

    Techniques: Sequencing, Reverse Transcription Polymerase Chain Reaction