pcr clean up  (Beckman Coulter)


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    Structured Review

    Beckman Coulter pcr clean up
    Library QC Tape station electropherogram. <t>Nextera</t> <t>Post-PCR</t> libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Pcr Clean Up, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up/product/Beckman Coulter
    Average 93 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2020-09
    93/100 stars

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    1) Product Images from "Improved workflows for high throughput library preparation using the transposome-based nextera system"

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-13-104

    Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Figure Legend Snippet: Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Techniques Used: Polymerase Chain Reaction, Construct, Produced

    Related Articles

    Multiplex Assay:

    Article Title: DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
    Article Snippet: .. Two independent PCR reactions with multiplex oligos for Illumina sequencing were performed and pooled for the final PCR clean-up by magnetic AMPure bead (Beckman Coulter) purification. ..

    Amplification:

    Article Title: Autism Spectrum Disorder (ASD) with and without Mental Regression Is Associated with Changes in the Fecal Microbiota
    Article Snippet: .. All PCRs were performed in 25 μL reaction volumes containing 12.5 μL 2X KAPA HiFi Hotstart ready mix (KAPA Biosystems, Woburn, MA, USA), 5 μL of each forward and reverse primers (1 μM) and 2.5 μL of extracted DNA (10 ng) under the following cycling conditions: initial denaturation at 95 °C for 3 min, followed by cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and elongation at 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR clean-up was performed using AMPure XP beads (Beckman Coulter, Indianapolis, IN, USA) to purify the 16S V3 and V4 amplicon away from free primers and primer dimer species. .. Then, the next step was the index PCR, in this step attaches dual indices and Illumina sequencing adapters using the Nextera XT Index Kit (Illumina, San Diego, CA, USA), PCR conditions were: 95 °C for 3 min; 8 cycles of 95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s; 72 °C for 5 min, and hold at 4 °C.

    Article Title: Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants
    Article Snippet: .. The PCR clean-up was performed with AMPure XP beads (Beckman Coulter, Copenhagen, Denmark), and confirmation of the correct amplicon size (ca. .. ~ 640 base pairs) was performed on a Bioanalyzer DNA 1000 chip (Agilent Technology, Santa Clara, CA, USA).

    Article Title: Perturbed DNA methylation by sustained overexpression of Gadd45b induces chromatin disorganization, DNA strand breaks and dopaminergic neuron death in mice
    Article Snippet: .. PCR clean-up after the final library amplification was performed using a 1.45x beads:sample ratio of Agencourt® AMPure® XP (Beckman Coulter). .. DNA concentration of the pools was measured using the Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific).

    Purification:

    Article Title: DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
    Article Snippet: .. Two independent PCR reactions with multiplex oligos for Illumina sequencing were performed and pooled for the final PCR clean-up by magnetic AMPure bead (Beckman Coulter) purification. ..

    Polymerase Chain Reaction:

    Article Title: Autism Spectrum Disorder (ASD) with and without Mental Regression Is Associated with Changes in the Fecal Microbiota
    Article Snippet: .. All PCRs were performed in 25 μL reaction volumes containing 12.5 μL 2X KAPA HiFi Hotstart ready mix (KAPA Biosystems, Woburn, MA, USA), 5 μL of each forward and reverse primers (1 μM) and 2.5 μL of extracted DNA (10 ng) under the following cycling conditions: initial denaturation at 95 °C for 3 min, followed by cycles of denaturation at 95 °C for 30 s, annealing at 55 °C for 30 s, and elongation at 72 °C for 30 s, with a final extension at 72 °C for 5 min. PCR clean-up was performed using AMPure XP beads (Beckman Coulter, Indianapolis, IN, USA) to purify the 16S V3 and V4 amplicon away from free primers and primer dimer species. .. Then, the next step was the index PCR, in this step attaches dual indices and Illumina sequencing adapters using the Nextera XT Index Kit (Illumina, San Diego, CA, USA), PCR conditions were: 95 °C for 3 min; 8 cycles of 95 °C for 30 s, 55 °C for 30 s, 72 °C for 30 s; 72 °C for 5 min, and hold at 4 °C.

    Article Title: DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
    Article Snippet: .. Two independent PCR reactions with multiplex oligos for Illumina sequencing were performed and pooled for the final PCR clean-up by magnetic AMPure bead (Beckman Coulter) purification. ..

    Article Title: Mouse Vendor Influence on the Bacterial and Viral Gut Composition Exceeds the Effect of Diet
    Article Snippet: .. Cycling conditions applied were: 72 °C for 3 min., 95 °C for 30 s, 16 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, followed by final step at 72 °C for 5 min. PCR clean-up: 25 µL PCR product was mixed with AMPure XP beads (Beckman Coulter Genomic, USA), and incubated for 5 min. at RT and mounted to the magnetic stand for 2 min before continuing. .. 27 µL of PCR-grade water was added, incubated at RT for 2 min., and mounted to a magnetic stand for 2 min. before sampling of 25 µL clean DNA products.

    Article Title: Probiotic supplementation restores normal microbiota composition and function in antibiotic-treated and in caesarean-born infants
    Article Snippet: .. The PCR clean-up was performed with AMPure XP beads (Beckman Coulter, Copenhagen, Denmark), and confirmation of the correct amplicon size (ca. .. ~ 640 base pairs) was performed on a Bioanalyzer DNA 1000 chip (Agilent Technology, Santa Clara, CA, USA).

    Article Title: Perturbed DNA methylation by sustained overexpression of Gadd45b induces chromatin disorganization, DNA strand breaks and dopaminergic neuron death in mice
    Article Snippet: .. PCR clean-up after the final library amplification was performed using a 1.45x beads:sample ratio of Agencourt® AMPure® XP (Beckman Coulter). .. DNA concentration of the pools was measured using the Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Integration of Kinase and Calcium Signaling at the Level of Chromatin Underlies Inducible Gene Activation in T Cells
    Article Snippet: .. A PCR clean-up was then performed using AMPure XP beads (Beckman Coulter), and the small fragments were then resuspended in 32.5 μl of resuspension buffer (provided in the Nextera kit). .. DNA was quantified using a Qubit fluorometer (Life Technologies), and library sizes were then determined using TapeStation (Agilent Technologies).

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system
    Article Snippet: .. Thermocycling was carried out on a Tetrad (Bio-Rad, 1000 Alfred Nobel Drive, Hercules, CA, 94547, USA) with the following standard Nextera parameters: PCR clean-up was performed following the Nextera protocol using a 0.6:1 ratio of AMPure XP® (Beckman Coulter) to PCR reaction. .. Reactions were eluted with EB (Qiagen).

    Article Title: Impacts of resistant starch and wheat bran consumption on enteric inflammation in relation to colonic bacterial community structures and short-chain fatty acid concentrations in mice
    Article Snippet: .. A PCR clean-up using AMPure XP (Beckman Coulter, Inc.) beads on a magnetic stand was performed on the 500 bp products, and an indexing PCR was used to add dual indices to each sample. .. Conditions included 5 µL of DNA, 5 µL of each index primer (specific non-repeating pair per sample) and 25 µL of 2× KAPA Hifi HotStart Ready mix and nuclease-free water (Qiagen Inc.) to a final volume of 50 µL per sample.

    Incubation:

    Article Title: Mouse Vendor Influence on the Bacterial and Viral Gut Composition Exceeds the Effect of Diet
    Article Snippet: .. Cycling conditions applied were: 72 °C for 3 min., 95 °C for 30 s, 16 cycles of 95 °C for 30 s, 55 °C for 30 s, and 72 °C for 30 s, followed by final step at 72 °C for 5 min. PCR clean-up: 25 µL PCR product was mixed with AMPure XP beads (Beckman Coulter Genomic, USA), and incubated for 5 min. at RT and mounted to the magnetic stand for 2 min before continuing. .. 27 µL of PCR-grade water was added, incubated at RT for 2 min., and mounted to a magnetic stand for 2 min. before sampling of 25 µL clean DNA products.

    Sequencing:

    Article Title: DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
    Article Snippet: .. Two independent PCR reactions with multiplex oligos for Illumina sequencing were performed and pooled for the final PCR clean-up by magnetic AMPure bead (Beckman Coulter) purification. ..

    Similar Products

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Beckman Coulter pcr clean up
    Library QC Tape station electropherogram. <t>Nextera</t> <t>Post-PCR</t> libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).
    Pcr Clean Up, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up/product/Beckman Coulter
    Average 93 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    pcr clean up - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Journal: BMC Biotechnology

    Article Title: Improved workflows for high throughput library preparation using the transposome-based nextera system

    doi: 10.1186/1472-6750-13-104

    Figure Lengend Snippet: Library QC Tape station electropherogram. Nextera Post-PCR libraries constructed with a range of concentrations (post-normalisation) and gDNA from four different genomes: Mycobacterium tuberculosis (purple), Escherichia coli (blue), Clostridium difficile (red) and Plasmodium falciparum (green). Libraries were constructed using the standard Nextera protocol (A) . Evaluation of the Axyprep Mag Normaliser kit (B) : two individual C. difficile Nextera libraries were constructed using the standard Illumina protocol (light/dark green) and two with our normalisation workflow (light/dark purple). Where the standard library had very short inserts, our method produced a library with the normal size distribution. Evaluation of C. difficile Nextera Post-PCR libraries constructed using varying volume Nextera reactions (C) : standard (purple), half-volume (green), quarter-volume (red) and one-eighth volume (blue). Size distribution profiles of libraries constructed using normalisation followed by reaction E (D) : M. tuberculosis (purple), E. coli (blue), C. difficile (red) and P. falciparum (green).

    Article Snippet: Thermocycling was carried out on a Tetrad (Bio-Rad, 1000 Alfred Nobel Drive, Hercules, CA, 94547, USA) with the following standard Nextera parameters: PCR clean-up was performed following the Nextera protocol using a 0.6:1 ratio of AMPure XP® (Beckman Coulter) to PCR reaction.

    Techniques: Polymerase Chain Reaction, Construct, Produced