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Axygen pcr clean up
Pcr Clean Up, supplied by Axygen, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up/product/Axygen
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pcr clean up - by Bioz Stars, 2020-09
92/100 stars

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Polymerase Chain Reaction:

Article Title: High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee (Apis mellifera) Embryos
Article Snippet: .. PCR products were purified by a PCR clean-up (Axygen) kit. .. In vitro transcription was performed with the HighMAXIscript SP6/T7 RNA in vitro transcription kit (Ambion, USA) according to the manufacturer’s instruction.

Article Title: Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
Article Snippet: .. Miniprep, maxiprep, gel extraction, and PCR clean-up All were performed using the kits purchased from Axygen (Union City, CA), according to the manufacturer's directions. .. DNA digestion and fragment purification Vector DNA was isolated from overnight E. coli bacterial cultures.

Article Title: Specific amino acids in HIV-1 Vpr are significantly associated with differences in patient neurocognitive status
Article Snippet: .. PCR clean-up was completed using 30 μL per sample of the AxyPrep™ Mag PCR Clean-Up procedure (Axygen® Cat # MAG-PCR-CL-5) as described by the manufacturer. .. Libraries were normalized using the Quant-iT™ dsDNA Assay procedure, High Sensitivity (Invitrogen Cat # ), and validated on an Agilent Technology 2100 Bioanalyzer using the Agilent High Sensitivity DNA procedure as previously described (Agilent Technologies Cat # 5067-4626).

Gel Extraction:

Article Title: Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen
Article Snippet: .. Miniprep, maxiprep, gel extraction, and PCR clean-up All were performed using the kits purchased from Axygen (Union City, CA), according to the manufacturer's directions. .. DNA digestion and fragment purification Vector DNA was isolated from overnight E. coli bacterial cultures.

Purification:

Article Title: High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee (Apis mellifera) Embryos
Article Snippet: .. PCR products were purified by a PCR clean-up (Axygen) kit. .. In vitro transcription was performed with the HighMAXIscript SP6/T7 RNA in vitro transcription kit (Ambion, USA) according to the manufacturer’s instruction.

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    Axygen pcr clean up kit
    Comparison of the sensitivities of mutation detection by <t>PCR</t> / RNP , PCR /T7 EI and direct Sanger sequencing. (a) The sg‐Os PDS ‐1 target site is located in exon1 of Os PDS . The PAM sequence is highlighted in red. ‘D1’ indicates a 1 bp deletion at the target site. (b c) Mixtures of WT and D1 PCR amplicons in different ratios were treated by PCR / RNP and PCR /T7 EI . (d) <t>DNA</t> sequence of the PCR amplicons surrounding the sg‐Os PDS ‐1 target site. The sg RNA sequence and 12 consecutive T's in the amplicons are highlighted in red and blue, respectively. (e) Mixtures of different proportions of WT and D1 PCR amplicons sequenced by the Sanger method.
    Pcr Clean Up Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/Axygen
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    89
    Axygen axyprep mag pcr clean up kit
    Comparison of the sensitivities of mutation detection by <t>PCR</t> / RNP , PCR /T7 EI and direct Sanger sequencing. (a) The sg‐Os PDS ‐1 target site is located in exon1 of Os PDS . The PAM sequence is highlighted in red. ‘D1’ indicates a 1 bp deletion at the target site. (b c) Mixtures of WT and D1 PCR amplicons in different ratios were treated by PCR / RNP and PCR /T7 EI . (d) <t>DNA</t> sequence of the PCR amplicons surrounding the sg‐Os PDS ‐1 target site. The sg RNA sequence and 12 consecutive T's in the amplicons are highlighted in red and blue, respectively. (e) Mixtures of different proportions of WT and D1 PCR amplicons sequenced by the Sanger method.
    Axyprep Mag Pcr Clean Up Kit, supplied by Axygen, used in various techniques. Bioz Stars score: 89/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axyprep mag pcr clean up kit/product/Axygen
    Average 89 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    axyprep mag pcr clean up kit - by Bioz Stars, 2020-09
    89/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of the sensitivities of mutation detection by PCR / RNP , PCR /T7 EI and direct Sanger sequencing. (a) The sg‐Os PDS ‐1 target site is located in exon1 of Os PDS . The PAM sequence is highlighted in red. ‘D1’ indicates a 1 bp deletion at the target site. (b c) Mixtures of WT and D1 PCR amplicons in different ratios were treated by PCR / RNP and PCR /T7 EI . (d) DNA sequence of the PCR amplicons surrounding the sg‐Os PDS ‐1 target site. The sg RNA sequence and 12 consecutive T's in the amplicons are highlighted in red and blue, respectively. (e) Mixtures of different proportions of WT and D1 PCR amplicons sequenced by the Sanger method.

    Journal: Plant Biotechnology Journal

    Article Title: Genotyping genome‐edited mutations in plants using CRISPR ribonucleoprotein complexes

    doi: 10.1111/pbi.12938

    Figure Lengend Snippet: Comparison of the sensitivities of mutation detection by PCR / RNP , PCR /T7 EI and direct Sanger sequencing. (a) The sg‐Os PDS ‐1 target site is located in exon1 of Os PDS . The PAM sequence is highlighted in red. ‘D1’ indicates a 1 bp deletion at the target site. (b c) Mixtures of WT and D1 PCR amplicons in different ratios were treated by PCR / RNP and PCR /T7 EI . (d) DNA sequence of the PCR amplicons surrounding the sg‐Os PDS ‐1 target site. The sg RNA sequence and 12 consecutive T's in the amplicons are highlighted in red and blue, respectively. (e) Mixtures of different proportions of WT and D1 PCR amplicons sequenced by the Sanger method.

    Article Snippet: Production of sgRNA and crRNA Templates for transcription of sgRNA and crRNA were amplified using corresponding primer sets (Table ) with high‐fidelity DNA polymerase and purified with PCR Clean‐Up Kit (Axygen). sgRNA and crRNA were synthesized using the HiScribe T7 In Vitro Transcription Kit (New England Biolabs) according to the manufacturer's instructions and purified by ethanol precipitation method.

    Techniques: Mutagenesis, Polymerase Chain Reaction, Sequencing

    ITE inhibits hypoxia- and tumour sphere-induced Oct4 elevation and leads to the differentiation of stem-like cancer cells. ( a ) U87 cells were seeded at a density of 2 × 10 5 per well of the six-well plate, cultured under normoxia (21% O 2 ) in DMEM for 24 h before being switched to the F10 medium and treated for 8 h with either 21% O 2 (normoxia) or 1% O 2 (hypoxia) in the presence of ITE (10 μM), Kyn (50 μM), Trp (1 μM) or aTrp. Cells were harvested and analysed using qRT–PCR for the mRNA levels of the POU5F1 . The data were expressed as mean±s.d. of triplicate measurements from one of three independent experiments. ( b,c ) Adherent parental U87 cells (U87 parental), U87 tumour sphere cells treated with vehicle (U87 sphere) or 10 μM ITE (U87 sphere+ITE) for varying duration were harvested and analysed using qRT–PCR for the mRNA levels of the indicated genes ( b ) and by immunoblotting (samples treated for 72 h) for the indicated proteins ( c ). ( d ) U87 cells grown in DMEM were transfected with a Scr siRNA or an AhR siRNA in combination with a plasmid containing EGFP only or EGFP + OCT4 . The transfected cells were simultaneously treated with DMSO (vehicle or −) or 10 μM ITE. Twenty-four hours after transfection, cells were cultured in a neural stem cell medium to induce the formation of tumour spheres. The pictures were taken 3 days after transfection. Scale bars, 100 μm. ( e ) The cultures described in d were quantified for the numbers of tumour spheres formed and analysed for the indicated proteins by immunoblotting. The data were expressed as mean±s.d. of three independent experiments. ( f ) Heat map generated from DNA microarray data for adherent parental U87 cells (U87 parental), U87 tumour sphere cells treated with vehicle (U87 sphere) or 10 μM ITE (U87 sphere+ITE) for 7 days. In a , b , e , The statistical significance of compared measurements was evaluated using the one-way ANOVA. * P

    Journal: Nature Communications

    Article Title: Tryptophan derivatives regulate the transcription of Oct4 in stem-like cancer cells

    doi: 10.1038/ncomms8209

    Figure Lengend Snippet: ITE inhibits hypoxia- and tumour sphere-induced Oct4 elevation and leads to the differentiation of stem-like cancer cells. ( a ) U87 cells were seeded at a density of 2 × 10 5 per well of the six-well plate, cultured under normoxia (21% O 2 ) in DMEM for 24 h before being switched to the F10 medium and treated for 8 h with either 21% O 2 (normoxia) or 1% O 2 (hypoxia) in the presence of ITE (10 μM), Kyn (50 μM), Trp (1 μM) or aTrp. Cells were harvested and analysed using qRT–PCR for the mRNA levels of the POU5F1 . The data were expressed as mean±s.d. of triplicate measurements from one of three independent experiments. ( b,c ) Adherent parental U87 cells (U87 parental), U87 tumour sphere cells treated with vehicle (U87 sphere) or 10 μM ITE (U87 sphere+ITE) for varying duration were harvested and analysed using qRT–PCR for the mRNA levels of the indicated genes ( b ) and by immunoblotting (samples treated for 72 h) for the indicated proteins ( c ). ( d ) U87 cells grown in DMEM were transfected with a Scr siRNA or an AhR siRNA in combination with a plasmid containing EGFP only or EGFP + OCT4 . The transfected cells were simultaneously treated with DMSO (vehicle or −) or 10 μM ITE. Twenty-four hours after transfection, cells were cultured in a neural stem cell medium to induce the formation of tumour spheres. The pictures were taken 3 days after transfection. Scale bars, 100 μm. ( e ) The cultures described in d were quantified for the numbers of tumour spheres formed and analysed for the indicated proteins by immunoblotting. The data were expressed as mean±s.d. of three independent experiments. ( f ) Heat map generated from DNA microarray data for adherent parental U87 cells (U87 parental), U87 tumour sphere cells treated with vehicle (U87 sphere) or 10 μM ITE (U87 sphere+ITE) for 7 days. In a , b , e , The statistical significance of compared measurements was evaluated using the one-way ANOVA. * P

    Article Snippet: An aliquot of the lysates (100 μl) was used as a control for the amount of input DNA after being purified using the PCR clean-up kit (Axygen AP-PCR-50).

    Techniques: Cell Culture, Quantitative RT-PCR, Transfection, Plasmid Preparation, Generated, Microarray

    Determination of the co-transcription of the csa1, cas1, cas2 and cas4 genes. ( A ) Organization of csa1 (852 bp), cas1 (873 bp), cas2 (270 bp) and cas4 (528 bp) genes is depicted schematically. The locations of primers are indicated that were used for RT-PCR to determine co-transcription. The intergenic regions between csa1 and cas1 is 42 bp and between cas1 and cas2 is 110 bp; cas2 and cas4 are overlapped. ( B ) Fragments obtained in RT-PCR experiments carried out with the primers csa1 qF and cas1 qR (lane 1, product 1016 bp), csa1 qF and cas2 qR (lane 2), csa1 qF and cas4 qR (lane 3), cas1 qF and cas2 qR (lane 4, product 1151 bp), cas1 qF and cas4 qR (lane 5, product 1418 bp), and cas2 qF and cas4 qR (lane 6, product 376 bp). A DNA ladder (Trans 2K plus II) was run in lane L as a size marker.

    Journal: Nucleic Acids Research

    Article Title: Transcriptional regulator-mediated activation of adaptation genes triggers CRISPR de novo spacer acquisition

    doi: 10.1093/nar/gku1383

    Figure Lengend Snippet: Determination of the co-transcription of the csa1, cas1, cas2 and cas4 genes. ( A ) Organization of csa1 (852 bp), cas1 (873 bp), cas2 (270 bp) and cas4 (528 bp) genes is depicted schematically. The locations of primers are indicated that were used for RT-PCR to determine co-transcription. The intergenic regions between csa1 and cas1 is 42 bp and between cas1 and cas2 is 110 bp; cas2 and cas4 are overlapped. ( B ) Fragments obtained in RT-PCR experiments carried out with the primers csa1 qF and cas1 qR (lane 1, product 1016 bp), csa1 qF and cas2 qR (lane 2), csa1 qF and cas4 qR (lane 3), cas1 qF and cas2 qR (lane 4, product 1151 bp), cas1 qF and cas4 qR (lane 5, product 1418 bp), and cas2 qF and cas4 qR (lane 6, product 376 bp). A DNA ladder (Trans 2K plus II) was run in lane L as a size marker.

    Article Snippet: Polymerase chain reaction (PCR) products were purified by using an Axygen PCR Clean-up Kit, and DNA bands fractionated from the agarose gel were extracted by using an Axygen DNA extraction kit.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Marker

    Effect of Csa3a on the expression of ac as genes. ( A ) PCR verification of the csa3a -deletion mutant. PCR products obtained using specific primers located upstream and downstream of csa3a gene from genomic DNAs of Sulfolobus islandicus E233S (wild-type) and the csa3a -deletion strain (Δ csa3a ). A DNA ladder was run in lane L as a size marker. ( B ) The relative transcription levels of csa3a, csa1, cas1, cas2 and cas4 in S. islandicus csa3a- overexpression (OE csa3a ) and csa3a -deletion (Δ csa3a ) strains after normalization to the level in S. islandicus E233S (wild-type) carrying empty vector pSeSD. ( C ) Western blot analysis of Csa3a, Csa1 and Cas1 protein expression levels in S. islandicus E233S (wild-type), csa3a overexpression (OE csa3a ) and csa3a -deletion (Δ csa3a ) strains. Antiserum against PCNA3 was used as a loading control.

    Journal: Nucleic Acids Research

    Article Title: Transcriptional regulator-mediated activation of adaptation genes triggers CRISPR de novo spacer acquisition

    doi: 10.1093/nar/gku1383

    Figure Lengend Snippet: Effect of Csa3a on the expression of ac as genes. ( A ) PCR verification of the csa3a -deletion mutant. PCR products obtained using specific primers located upstream and downstream of csa3a gene from genomic DNAs of Sulfolobus islandicus E233S (wild-type) and the csa3a -deletion strain (Δ csa3a ). A DNA ladder was run in lane L as a size marker. ( B ) The relative transcription levels of csa3a, csa1, cas1, cas2 and cas4 in S. islandicus csa3a- overexpression (OE csa3a ) and csa3a -deletion (Δ csa3a ) strains after normalization to the level in S. islandicus E233S (wild-type) carrying empty vector pSeSD. ( C ) Western blot analysis of Csa3a, Csa1 and Cas1 protein expression levels in S. islandicus E233S (wild-type), csa3a overexpression (OE csa3a ) and csa3a -deletion (Δ csa3a ) strains. Antiserum against PCNA3 was used as a loading control.

    Article Snippet: Polymerase chain reaction (PCR) products were purified by using an Axygen PCR Clean-up Kit, and DNA bands fractionated from the agarose gel were extracted by using an Axygen DNA extraction kit.

    Techniques: Expressing, Polymerase Chain Reaction, Mutagenesis, Marker, Over Expression, Plasmid Preparation, Western Blot