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Promega pcr clean up systems
Pcr Clean Up Systems, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up systems/product/Promega
Average 99 stars, based on 8 article reviews
Price from $9.99 to $1999.99
pcr clean up systems - by Bioz Stars, 2020-01
99/100 stars

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Amplification:

Article Title: Drinking water microbiome assembly induced by water stagnation
Article Snippet: .. The amplified products were purified with Promega PCR clean-up systems. ..

DNA Purification:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: .. DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA). .. Levan and DFA IV standards were purchased from Realbiotech Co., Ltd. (Gongju, Korea).

Cell Culture:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA). .. Yeast cells were cultured in YPD (1% yeast extract, 2% Bacto peptone, and 2% glucose).

Purification:

Article Title: Drinking water microbiome assembly induced by water stagnation
Article Snippet: .. The amplified products were purified with Promega PCR clean-up systems. ..

Polymerase Chain Reaction:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: .. DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA). .. Levan and DFA IV standards were purchased from Realbiotech Co., Ltd. (Gongju, Korea).

Article Title: Drinking water microbiome assembly induced by water stagnation
Article Snippet: .. The amplified products were purified with Promega PCR clean-up systems. ..

Expressing:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: The parent strain, Saccharomyces cerevisiae (Y2805gs, Mat α pep4::HIS3, prb1, can1, his3-200, ura3-52, gal80::Tc190, suc2::Tc190 ) was used as the gene expression host to avoid sucrose hydrolysis . .. DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA).

Sequencing:

Article Title: Drinking water microbiome assembly induced by water stagnation
Article Snippet: Paragraph title: Illumina sequencing ... The amplified products were purified with Promega PCR clean-up systems.

Direct Fluorescent Antibody Test:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA). .. Levan and DFA IV standards were purchased from Realbiotech Co., Ltd. (Gongju, Korea).

Recombinant:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: Strains, chemicals, and media E. coli DH5α [F− lac ZΔM15 hsd R17(r- m-) gyr A36] was used for the recombinant DNA techniques. .. DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA).

Molecular Weight:

Article Title: Direct Production of Difructose Anhydride IV from Sucrose by Co-fermentation of Recombinant Yeasts
Article Snippet: DNA purification was conducted using the Wizard® SV Gel and PCR Clean-Up Systems (Promega, Madison, WI, USA). .. Various molecular weight dextran standards (12,000 to 670,000 Da) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

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    Promega wizard sv gel and pcr clean up system
    Ten-2-LI immunoperoxidase analysis of human dental pulp and conventional <t>RT-PCR.</t> In A-B, odontoblast layer at high magnification with intense Ten-2-LI (arrow). In C, 1.5% agarose gel stained with bromide ethidium exhibiting Ten-2, <t>TCAP-2</t> and beta-actin RT-PCR-amplified products using total RNA extracted from human third molar dental pulp. Abbreviations: P, dental pulp; TCAP-2, teneurin C-terminal associated peptide-2; Ten-2, teneurin 2; β-actin, beta actin.
    Wizard Sv Gel And Pcr Clean Up System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 2159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard sv gel and pcr clean up system/product/Promega
    Average 90 stars, based on 2159 article reviews
    Price from $9.99 to $1999.99
    wizard sv gel and pcr clean up system - by Bioz Stars, 2020-01
    90/100 stars
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    Ten-2-LI immunoperoxidase analysis of human dental pulp and conventional RT-PCR. In A-B, odontoblast layer at high magnification with intense Ten-2-LI (arrow). In C, 1.5% agarose gel stained with bromide ethidium exhibiting Ten-2, TCAP-2 and beta-actin RT-PCR-amplified products using total RNA extracted from human third molar dental pulp. Abbreviations: P, dental pulp; TCAP-2, teneurin C-terminal associated peptide-2; Ten-2, teneurin 2; β-actin, beta actin.

    Journal: PLoS ONE

    Article Title: Teneurin-2 presence in rat and human odontoblasts

    doi: 10.1371/journal.pone.0184794

    Figure Lengend Snippet: Ten-2-LI immunoperoxidase analysis of human dental pulp and conventional RT-PCR. In A-B, odontoblast layer at high magnification with intense Ten-2-LI (arrow). In C, 1.5% agarose gel stained with bromide ethidium exhibiting Ten-2, TCAP-2 and beta-actin RT-PCR-amplified products using total RNA extracted from human third molar dental pulp. Abbreviations: P, dental pulp; TCAP-2, teneurin C-terminal associated peptide-2; Ten-2, teneurin 2; β-actin, beta actin.

    Article Snippet: In order to confirm that the RT-PCR products were from Ten-2 or TCAP-2 mRNA amplification, some samples were purified from gel (A9282, Wizard SV gel and PCR Clean-Up System, Promega, WI, USA) and subcloned in plasmids (A1380, pGEM-T Easy Vector System, Promega, WI, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Amplification

    Ten-2-LI immunoperoxidase analysis in dental pulp of rat mature molar tooth and conventional RT-PCR. Note presence of intense immunolabeling to Ten-2 in odontoblasts (large arrow), positioned in roof of coronal pulp (A, high magnification in B), lateral wall (C, high magnification in D) and in radicular pulp (E). In F, observe presence of discreet immunolabeled cells subjacent to odontoblast layer (small arrow) and no immunoreactive cells in center of dental pulp. In G, expression of Teneurin-2 and TCAP-2 in 1.5% agarose gel using total RNA extracted from rat molar pulp during crown formation. Abbreviations: CRZ, cell-rich zone; D, dentin; P, dental pulp; RP, radicular pulp; TCAP-2, teneurin C-terminal associated peptide-2; Ten-2, teneurin 2.

    Journal: PLoS ONE

    Article Title: Teneurin-2 presence in rat and human odontoblasts

    doi: 10.1371/journal.pone.0184794

    Figure Lengend Snippet: Ten-2-LI immunoperoxidase analysis in dental pulp of rat mature molar tooth and conventional RT-PCR. Note presence of intense immunolabeling to Ten-2 in odontoblasts (large arrow), positioned in roof of coronal pulp (A, high magnification in B), lateral wall (C, high magnification in D) and in radicular pulp (E). In F, observe presence of discreet immunolabeled cells subjacent to odontoblast layer (small arrow) and no immunoreactive cells in center of dental pulp. In G, expression of Teneurin-2 and TCAP-2 in 1.5% agarose gel using total RNA extracted from rat molar pulp during crown formation. Abbreviations: CRZ, cell-rich zone; D, dentin; P, dental pulp; RP, radicular pulp; TCAP-2, teneurin C-terminal associated peptide-2; Ten-2, teneurin 2.

    Article Snippet: In order to confirm that the RT-PCR products were from Ten-2 or TCAP-2 mRNA amplification, some samples were purified from gel (A9282, Wizard SV gel and PCR Clean-Up System, Promega, WI, USA) and subcloned in plasmids (A1380, pGEM-T Easy Vector System, Promega, WI, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Immunolabeling, Expressing, Agarose Gel Electrophoresis