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MACHEREY NAGEL pcr clean up spin columns
Real-time <t>PCR</t> analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target <t>amplicon,</t> the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
Pcr Clean Up Spin Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr clean up spin columns/product/MACHEREY NAGEL
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pcr clean up spin columns - by Bioz Stars, 2020-09
85/100 stars

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1) Product Images from "In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases"

Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkx116

Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
Figure Legend Snippet: Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.

Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Amplification

Related Articles

Polymerase Chain Reaction:

Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases
Article Snippet: .. PCR amplicon DNA was isolated from gel slices using NuceloSpin Gel and PCR Clean-up spin columns, eluting in 30 μl volume (Macherey-Nagel). .. PCR amplicon concentrations were determined using Qubit Fluorometer (Thermo Fisher Scientific), and molar concentrations calculated using pre-edited amplicon size.

Article Title: CDK5 Is Essential for Soluble Amyloid ?-Induced Degradation of GKAP and Remodeling of the Synaptic Actin Cytoskeleton
Article Snippet: .. PCR products were purified on PCR clean-up spin columns (Macherey-Nagel, Dueren, Germany) and the DNA was digested with DpnI (20 U) for 90 min at 37°C, after which Dpn1 was inactivated (65°C, 10 min). .. DNA was extracted (phenol/chloroform), precipitated and inoculated into transformed, electro-competent bacteria (Invitrogen).

Isolation:

Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases
Article Snippet: .. PCR amplicon DNA was isolated from gel slices using NuceloSpin Gel and PCR Clean-up spin columns, eluting in 30 μl volume (Macherey-Nagel). .. PCR amplicon concentrations were determined using Qubit Fluorometer (Thermo Fisher Scientific), and molar concentrations calculated using pre-edited amplicon size.

Amplification:

Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases
Article Snippet: .. PCR amplicon DNA was isolated from gel slices using NuceloSpin Gel and PCR Clean-up spin columns, eluting in 30 μl volume (Macherey-Nagel). .. PCR amplicon concentrations were determined using Qubit Fluorometer (Thermo Fisher Scientific), and molar concentrations calculated using pre-edited amplicon size.

Purification:

Article Title: CDK5 Is Essential for Soluble Amyloid ?-Induced Degradation of GKAP and Remodeling of the Synaptic Actin Cytoskeleton
Article Snippet: .. PCR products were purified on PCR clean-up spin columns (Macherey-Nagel, Dueren, Germany) and the DNA was digested with DpnI (20 U) for 90 min at 37°C, after which Dpn1 was inactivated (65°C, 10 min). .. DNA was extracted (phenol/chloroform), precipitated and inoculated into transformed, electro-competent bacteria (Invitrogen).

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  • 85
    MACHEREY NAGEL pcr clean up spin columns
    Real-time <t>PCR</t> analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target <t>amplicon,</t> the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
    Pcr Clean Up Spin Columns, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up spin columns/product/MACHEREY NAGEL
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcr clean up spin columns - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    94
    MACHEREY NAGEL pcr clean up kit
    Real-time <t>PCR</t> analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target <t>amplicon,</t> the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
    Pcr Clean Up Kit, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 94/100, based on 844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/MACHEREY NAGEL
    Average 94 stars, based on 844 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    84
    MACHEREY NAGEL silica membrane spin column technique
    Real-time <t>PCR</t> analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target <t>amplicon,</t> the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.
    Silica Membrane Spin Column Technique, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/silica membrane spin column technique/product/MACHEREY NAGEL
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    silica membrane spin column technique - by Bioz Stars, 2020-09
    84/100 stars
      Buy from Supplier

    Image Search Results


    Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.

    Journal: Nucleic Acids Research

    Article Title: In vivo cleavage specificity of Trypanosoma brucei editosome endonucleases

    doi: 10.1093/nar/gkx116

    Figure Lengend Snippet: Real-time PCR analysis shows loss of endonuclease expression and associated loss of editing. Relative RNA abundance is shown for KREN1, KREN2, KREN3 and never-edited mRNAs COI and ND4 (black bars), pre-edited mRNAs (white bars), and edited mRNAs (gray bars). For each target amplicon, the relative change in RNA abundance was determined by using telomerase reverse transcriptase (TERT) mRNA as an internal control, with each cell line compared relative to either 427wt or MGA control as indicated. ( A ) MGA cells have no large changes in mRNA abundance relative to 427wt, with the exception of the complete loss of CYb editing. As the remaining cell lines are compared to MGA, CYb is excluded from those analyses. ( B ) KREN1 only cells have no detectable KREN2 or KREN3 mRNA, and a broad loss of RNA editing. ( C ) KREN2 only cells have no detectable KREN1 or KREN3 mRNA, and a broad loss of RNA editing. ( D ) KREN3 only cells have no detectable KREN1 or KREN2 mRNA, and a broad loss of RNA editing, with COII editing notably retained. ( E ) Triple null cells have no detectable KREN1, KREN2, or KREN3 mRNA, and a broad loss of RNA editing. ( F ) KREN2 null cells have no detectable KREN2 mRNA, and a broad loss of RNA editing with some amount of COII and ND7 editing retained. ( G ) KREN3 null cells have no detectable KREN3 mRNA, and a loss of COII RNA editing with variable amounts of other edited mRNAs retained.

    Article Snippet: PCR amplicon DNA was isolated from gel slices using NuceloSpin Gel and PCR Clean-up spin columns, eluting in 30 μl volume (Macherey-Nagel).

    Techniques: Real-time Polymerase Chain Reaction, Expressing, Amplification