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GE Healthcare pcr clean up kit
Pcr Clean Up Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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Nucleic Acid Electrophoresis:

Article Title: Pbx Proteins in Cryptococcus neoformans Cell Wall Remodeling and Capsule Assembly
Article Snippet: .. Detergent was removed by using an SDS-PAGE cleanup kit (GE Healthcare) before protein gel electrophoresis. ..

Article Title: An enriched environment elevates corticosteroid receptor levels in the hippocampus and restores cognitive function in a rat model of chronic cerebral hypoperfusion.
Article Snippet: .. An enriched environment (EE) is beneficial for modifying certain behaviors, particularly those involving complex cognitive functions. .. An enriched environment (EE) is beneficial for modifying certain behaviors, particularly those involving complex cognitive functions.

Article Title: Ion-Pair Interaction and Hydrogen Bonds as Main Features of Protein Thermostability in Mutated T1 Recombinant Lipase Originating from Geobacilluszalihae
Article Snippet: .. All lipases were successfully purified to homogeneity, as shown by the presence of a single band at approximately 44 kDa when analyzed by SDS-polyacrylamide gel electrophoresis ( ). .. Optimum Temperature and Thermostability Study of Mutated HT1 Lipases All mutants displayed high activity at a temperature range of 60 to 80 °C ( ).

Flow Cytometry:

Article Title: Evidence for the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes
Article Snippet: .. The flow-through fractions were precipitated with the SDS/PAGE Clean-Up kit (Amersham Biosciences) and analysed by Western blotting. ..

Purification:

Article Title: Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression
Article Snippet: .. During cell disruption and purification, protein samples were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue staining. .. Finally, protein concentration was determined using the Bradford assay and bovine serum albumin (BSA) as standard.

Article Title: Ion-Pair Interaction and Hydrogen Bonds as Main Features of Protein Thermostability in Mutated T1 Recombinant Lipase Originating from Geobacilluszalihae
Article Snippet: .. All lipases were successfully purified to homogeneity, as shown by the presence of a single band at approximately 44 kDa when analyzed by SDS-polyacrylamide gel electrophoresis ( ). .. Optimum Temperature and Thermostability Study of Mutated HT1 Lipases All mutants displayed high activity at a temperature range of 60 to 80 °C ( ).

Polyacrylamide Gel Electrophoresis:

Article Title: A Combinatorial MAP Code Dictates Polarized Microtubule Transport
Article Snippet: .. The resulting preparations were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE). .. Pull-down AssaysPull-down assays were performed with either sfGFP-DCLK or sfGFP-MAP9 tagged at the C-terminal end with a FLAG epitope.

Article Title: Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression
Article Snippet: .. During cell disruption and purification, protein samples were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue staining. .. Finally, protein concentration was determined using the Bradford assay and bovine serum albumin (BSA) as standard.

Western Blot:

Article Title: Evidence for the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes
Article Snippet: .. The flow-through fractions were precipitated with the SDS/PAGE Clean-Up kit (Amersham Biosciences) and analysed by Western blotting. ..

Staining:

Article Title: Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression
Article Snippet: .. During cell disruption and purification, protein samples were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue staining. .. Finally, protein concentration was determined using the Bradford assay and bovine serum albumin (BSA) as standard.

SDS Page:

Article Title: Pbx Proteins in Cryptococcus neoformans Cell Wall Remodeling and Capsule Assembly
Article Snippet: .. Detergent was removed by using an SDS-PAGE cleanup kit (GE Healthcare) before protein gel electrophoresis. ..

Article Title: Evidence for the occurrence of membrane-type serine protease 1/matriptase on the basolateral sides of enterocytes
Article Snippet: .. The flow-through fractions were precipitated with the SDS/PAGE Clean-Up kit (Amersham Biosciences) and analysed by Western blotting. ..

Article Title: Separate mechanisms act concurrently to shed and release the prion protein from the cell
Article Snippet: .. After PNGase F-treatment as indicated in the specific experiments, proteins were precipitated with acetone or with trichloroacetic acid (TCA) using the PlusOne SDS-PAGE Clean-Up Kit (Amersham Biosciences, Uppsala, Sweden) according to the manufacturer’s protocol. .. The resulting protein pellet was prepared for electrophoresis as described above.

Article Title: A Combinatorial MAP Code Dictates Polarized Microtubule Transport
Article Snippet: .. The resulting preparations were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE). .. Pull-down AssaysPull-down assays were performed with either sfGFP-DCLK or sfGFP-MAP9 tagged at the C-terminal end with a FLAG epitope.

Article Title: Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression
Article Snippet: .. During cell disruption and purification, protein samples were analyzed by SDS polyacrylamide gel electrophoresis (SDS-PAGE) with Coomassie blue staining. .. Finally, protein concentration was determined using the Bradford assay and bovine serum albumin (BSA) as standard.

Article Title: Deep Amino Acid Sequencing of Native Brain GABAA Receptors Using High-Resolution Mass Spectrometry *
Article Snippet: .. Briefly, the proteins were first precipitated using an SDS-PAGE Clean-up Kit (GE Healthcare Life Sciences) according to the manufacturer's protocol. .. The precipitated proteins were solubilized in 20 μl of 8 m urea, 5% RapiGest™ (Waters, Milford MA), 100 mm Tris, pH 8.5 for 30 min at 37 °C with agitation.

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  • 99
    GE Healthcare 2 de clean up kit
    Comparative proteomic and molecular analyses reveal that PhoP is subject to autoregulation and controls ftsA transcription. (A) Functional classification of differentially expressed proteins in the phoP Xcc mutant. The ΔphoP-pphoP mutant was grown in MMX medium with 0.05% L-arabinose or 2 mM IPTG. A comparative proteomic analysis was carried out using <t>2-DE</t> with MALDI-TOF-MS/MS. The 2-DE analysis was repeated three independent times, each with two technical replicates. (B–D) phoP Xcc regulates the transcription of its own operon and ftsA Xcc . qRT-PCR was used to measure the mRNA levels of phoP Xcc (plasmid-borne copy), phoQ Xcc (chromosomal copy) and ftsA Xcc . Total RNA was extracted from the phoP mutant (Δpho P -pphoP) that was grown in MMX medium containing IPTG (2 mM, for repression of the phoP Xcc expression) or L-arabinose (0.05%, for induction of the phoP Xcc expression). tmRNA transcripts were used as an internal control. The experiments were replicated three independent times, each with three technical repeats. A representative experiment is shown. (E and F) PhoP Xcc binds to the 5′ promoter regions upstream of phoP Xcc and ftsA Xcc . Increasing amounts of the PhoP Xcc protein were tested in an EMSA. Increasing amounts of unlabeled probes were used as competitors to bind to PhoP Xcc protein extracts. The experiments were repeated three times, and a representative is shown. 2-DE, two-dimensional electrophoresis; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; MMX, minimal medium for Xanthomonas campestris ; qRT-PCR, quantitative real-time RT-PCR.
    2 De Clean Up Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 de clean up kit/product/GE Healthcare
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    2 de clean up kit - by Bioz Stars, 2020-09
    99/100 stars
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    99
    GE Healthcare exosap it pcr clean up kit
    Comparative proteomic and molecular analyses reveal that PhoP is subject to autoregulation and controls ftsA transcription. (A) Functional classification of differentially expressed proteins in the phoP Xcc mutant. The ΔphoP-pphoP mutant was grown in MMX medium with 0.05% L-arabinose or 2 mM IPTG. A comparative proteomic analysis was carried out using <t>2-DE</t> with MALDI-TOF-MS/MS. The 2-DE analysis was repeated three independent times, each with two technical replicates. (B–D) phoP Xcc regulates the transcription of its own operon and ftsA Xcc . qRT-PCR was used to measure the mRNA levels of phoP Xcc (plasmid-borne copy), phoQ Xcc (chromosomal copy) and ftsA Xcc . Total RNA was extracted from the phoP mutant (Δpho P -pphoP) that was grown in MMX medium containing IPTG (2 mM, for repression of the phoP Xcc expression) or L-arabinose (0.05%, for induction of the phoP Xcc expression). tmRNA transcripts were used as an internal control. The experiments were replicated three independent times, each with three technical repeats. A representative experiment is shown. (E and F) PhoP Xcc binds to the 5′ promoter regions upstream of phoP Xcc and ftsA Xcc . Increasing amounts of the PhoP Xcc protein were tested in an EMSA. Increasing amounts of unlabeled probes were used as competitors to bind to PhoP Xcc protein extracts. The experiments were repeated three times, and a representative is shown. 2-DE, two-dimensional electrophoresis; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; MMX, minimal medium for Xanthomonas campestris ; qRT-PCR, quantitative real-time RT-PCR.
    Exosap It Pcr Clean Up Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exosap it pcr clean up kit/product/GE Healthcare
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    exosap it pcr clean up kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare pcr clean up kit
    Comparative proteomic and molecular analyses reveal that PhoP is subject to autoregulation and controls ftsA transcription. (A) Functional classification of differentially expressed proteins in the phoP Xcc mutant. The ΔphoP-pphoP mutant was grown in MMX medium with 0.05% L-arabinose or 2 mM IPTG. A comparative proteomic analysis was carried out using <t>2-DE</t> with MALDI-TOF-MS/MS. The 2-DE analysis was repeated three independent times, each with two technical replicates. (B–D) phoP Xcc regulates the transcription of its own operon and ftsA Xcc . qRT-PCR was used to measure the mRNA levels of phoP Xcc (plasmid-borne copy), phoQ Xcc (chromosomal copy) and ftsA Xcc . Total RNA was extracted from the phoP mutant (Δpho P -pphoP) that was grown in MMX medium containing IPTG (2 mM, for repression of the phoP Xcc expression) or L-arabinose (0.05%, for induction of the phoP Xcc expression). tmRNA transcripts were used as an internal control. The experiments were replicated three independent times, each with three technical repeats. A representative experiment is shown. (E and F) PhoP Xcc binds to the 5′ promoter regions upstream of phoP Xcc and ftsA Xcc . Increasing amounts of the PhoP Xcc protein were tested in an EMSA. Increasing amounts of unlabeled probes were used as competitors to bind to PhoP Xcc protein extracts. The experiments were repeated three times, and a representative is shown. 2-DE, two-dimensional electrophoresis; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; MMX, minimal medium for Xanthomonas campestris ; qRT-PCR, quantitative real-time RT-PCR.
    Pcr Clean Up Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr clean up kit/product/GE Healthcare
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr clean up kit - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    Comparative proteomic and molecular analyses reveal that PhoP is subject to autoregulation and controls ftsA transcription. (A) Functional classification of differentially expressed proteins in the phoP Xcc mutant. The ΔphoP-pphoP mutant was grown in MMX medium with 0.05% L-arabinose or 2 mM IPTG. A comparative proteomic analysis was carried out using 2-DE with MALDI-TOF-MS/MS. The 2-DE analysis was repeated three independent times, each with two technical replicates. (B–D) phoP Xcc regulates the transcription of its own operon and ftsA Xcc . qRT-PCR was used to measure the mRNA levels of phoP Xcc (plasmid-borne copy), phoQ Xcc (chromosomal copy) and ftsA Xcc . Total RNA was extracted from the phoP mutant (Δpho P -pphoP) that was grown in MMX medium containing IPTG (2 mM, for repression of the phoP Xcc expression) or L-arabinose (0.05%, for induction of the phoP Xcc expression). tmRNA transcripts were used as an internal control. The experiments were replicated three independent times, each with three technical repeats. A representative experiment is shown. (E and F) PhoP Xcc binds to the 5′ promoter regions upstream of phoP Xcc and ftsA Xcc . Increasing amounts of the PhoP Xcc protein were tested in an EMSA. Increasing amounts of unlabeled probes were used as competitors to bind to PhoP Xcc protein extracts. The experiments were repeated three times, and a representative is shown. 2-DE, two-dimensional electrophoresis; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; MMX, minimal medium for Xanthomonas campestris ; qRT-PCR, quantitative real-time RT-PCR.

    Journal: Genetics

    Article Title: An Essential Regulatory System Originating from Polygenic Transcriptional Rewiring of PhoP-PhoQ of Xanthomonas campestris

    doi: 10.1534/genetics.117.200204

    Figure Lengend Snippet: Comparative proteomic and molecular analyses reveal that PhoP is subject to autoregulation and controls ftsA transcription. (A) Functional classification of differentially expressed proteins in the phoP Xcc mutant. The ΔphoP-pphoP mutant was grown in MMX medium with 0.05% L-arabinose or 2 mM IPTG. A comparative proteomic analysis was carried out using 2-DE with MALDI-TOF-MS/MS. The 2-DE analysis was repeated three independent times, each with two technical replicates. (B–D) phoP Xcc regulates the transcription of its own operon and ftsA Xcc . qRT-PCR was used to measure the mRNA levels of phoP Xcc (plasmid-borne copy), phoQ Xcc (chromosomal copy) and ftsA Xcc . Total RNA was extracted from the phoP mutant (Δpho P -pphoP) that was grown in MMX medium containing IPTG (2 mM, for repression of the phoP Xcc expression) or L-arabinose (0.05%, for induction of the phoP Xcc expression). tmRNA transcripts were used as an internal control. The experiments were replicated three independent times, each with three technical repeats. A representative experiment is shown. (E and F) PhoP Xcc binds to the 5′ promoter regions upstream of phoP Xcc and ftsA Xcc . Increasing amounts of the PhoP Xcc protein were tested in an EMSA. Increasing amounts of unlabeled probes were used as competitors to bind to PhoP Xcc protein extracts. The experiments were repeated three times, and a representative is shown. 2-DE, two-dimensional electrophoresis; MALDI-TOF-MS, matrix-assisted laser desorption/ionization time of flight mass spectrometry; MMX, minimal medium for Xanthomonas campestris ; qRT-PCR, quantitative real-time RT-PCR.

    Article Snippet: The protein concentration was determined using a 2-DE Quant kit (GE Healthcare), followed by protein purification by a 2-DE clean-up kit (GE Healthcare).

    Techniques: Functional Assay, Mutagenesis, Mass Spectrometry, Quantitative RT-PCR, Plasmid Preparation, Expressing, Electrophoresis