Structured Review

Boehringer Mannheim pcr buffer
Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr buffer/product/Boehringer Mannheim
Average 92 stars, based on 164 article reviews
Price from $9.99 to $1999.99
pcr buffer - by Bioz Stars, 2020-07
92/100 stars

Images

1) Product Images from "Contaminations Occurring in Fungal PCR Assays"

Article Title: Contaminations Occurring in Fungal PCR Assays

Journal: Journal of Clinical Microbiology

doi:

Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.
Figure Legend Snippet: Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

Techniques Used: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Amplification, Marker, Labeling, Positive Control, Negative Control, Polymerase Chain Reaction

2) Product Images from "Gene Expression of Osteoprotegerin Ligand, Osteoprotegerin, and Receptor Activator of NF-?B in Giant Cell Tumor of Bone "

Article Title: Gene Expression of Osteoprotegerin Ligand, Osteoprotegerin, and Receptor Activator of NF-?B in Giant Cell Tumor of Bone

Journal: The American Journal of Pathology

doi:

Gene expression of OPGL, OPG, and RANK in GCT. A: Cycle-dependent PCR reactions were removed from 20 to 35 cycles at two- to three-cycle intervals and electrophoresed on 1.5% agarose gels stained with EtBr. The intensity of EtBr fluorescence was measured by densitometry and plotted as a function of cycle number to generate amplification curves for OPGL, OPG, and RANK PCR fragments. B: Expression of OPGL, OPG, and RANK mRNA in samples from five cases of GCT ( lanes 1–5 , cases 1–5, respectively), normal cancellous bone ( lane 6 ), and osteoblast-like osteosarcoma cells U2OS ( lane 7 ). The sizes of OPGL, RANK, OPG, and GAPDH PCR products were 486, 497, 324, and 206 bp, respectively. The GAPDH housekeeping gene determines the variation of loading in the gel.
Figure Legend Snippet: Gene expression of OPGL, OPG, and RANK in GCT. A: Cycle-dependent PCR reactions were removed from 20 to 35 cycles at two- to three-cycle intervals and electrophoresed on 1.5% agarose gels stained with EtBr. The intensity of EtBr fluorescence was measured by densitometry and plotted as a function of cycle number to generate amplification curves for OPGL, OPG, and RANK PCR fragments. B: Expression of OPGL, OPG, and RANK mRNA in samples from five cases of GCT ( lanes 1–5 , cases 1–5, respectively), normal cancellous bone ( lane 6 ), and osteoblast-like osteosarcoma cells U2OS ( lane 7 ). The sizes of OPGL, RANK, OPG, and GAPDH PCR products were 486, 497, 324, and 206 bp, respectively. The GAPDH housekeeping gene determines the variation of loading in the gel.

Techniques Used: Expressing, Polymerase Chain Reaction, Staining, Fluorescence, Amplification

3) Product Images from "PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora"

Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora

Journal: Applied and Environmental Microbiology

doi:

Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.
Figure Legend Snippet: Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.

Techniques Used: Amplification, Polymerase Chain Reaction

Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.
Figure Legend Snippet: Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.

Techniques Used: Amplification, Polymerase Chain Reaction

4) Product Images from "Changes in the Properties of Gap Junctions during Neuronal Differentiation of Hippocampal Progenitor Cells"

Article Title: Changes in the Properties of Gap Junctions during Neuronal Differentiation of Hippocampal Progenitor Cells

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.18-05-01753.1998

Determination of the connexin types expressed by differentiating neuroblasts by 7 DIV. A , Composite of gels from first-generation cDNA obtained by RT-PCR using universal primers, run on a 2% agarose gel, and visualized by ethidium bromide staining. Lanes 1, 3, and 5 show first-strand DNA from untreated cells, IL-7 alone, and IL-7 plus growth factors, respectively. Lanes 2 (untreated cells), 4 (IL-7), and 6 (IL-7 plus growth factors) are negative controls, run in PCR without reverse transcriptase to verify that genomic DNA was not present. Lane 7 is the PCR product of Cx33 from mouse testis, and lane 8 is its corresponding negative control. Lane 9 is the PCR product of Cx43 from rat heart, and lane 10 is its corresponding negative control. B , Presence of Cx33 and Cx43 confirmed by RT-PCR. Second-generation PCR product was obtained with universal primers. Lanes 1, 3, and 5 show the presence of restriction products of Cx43 after Hin cII was used. The product gave two bands for untreated, IL-7 alone, and IL-7 plus growth factors. Lane 9 is the Hin cII digest of the PCR product from the rat heart, which also gave the characteristic double band for Cx43. C , Lanes 1, 3, and 5 show the presence of the restriction product of Cx33 after Mse I was used. This enzyme gave two bands for IL-7 alone and IL-7 plus growth factors. Lane 7 is the PCR product of the Cx33 from mouse testis that had been cut with Mse I; two bands were formed. Sequencing the RT-PCR products revealed the presence of mouse Cx43 (99.1% identical) and Cx33 (98.2% identity with rat Cx33 sequence). Similar procedures applied with specific primers for Cx37 ( D , lanes 1, 2 ) and for Cx40 ( E , lanes 1, 2 ) in cells treated with IL-7 alone resulted in detection of a product of a 311 bp, consistent with the expression of Cx40. M , Molecular markers, PCR products of Cx37 (mouse brain; D, lane 1 ) and Cx40 (mouse heart; E, lane 1 ), respectively.
Figure Legend Snippet: Determination of the connexin types expressed by differentiating neuroblasts by 7 DIV. A , Composite of gels from first-generation cDNA obtained by RT-PCR using universal primers, run on a 2% agarose gel, and visualized by ethidium bromide staining. Lanes 1, 3, and 5 show first-strand DNA from untreated cells, IL-7 alone, and IL-7 plus growth factors, respectively. Lanes 2 (untreated cells), 4 (IL-7), and 6 (IL-7 plus growth factors) are negative controls, run in PCR without reverse transcriptase to verify that genomic DNA was not present. Lane 7 is the PCR product of Cx33 from mouse testis, and lane 8 is its corresponding negative control. Lane 9 is the PCR product of Cx43 from rat heart, and lane 10 is its corresponding negative control. B , Presence of Cx33 and Cx43 confirmed by RT-PCR. Second-generation PCR product was obtained with universal primers. Lanes 1, 3, and 5 show the presence of restriction products of Cx43 after Hin cII was used. The product gave two bands for untreated, IL-7 alone, and IL-7 plus growth factors. Lane 9 is the Hin cII digest of the PCR product from the rat heart, which also gave the characteristic double band for Cx43. C , Lanes 1, 3, and 5 show the presence of the restriction product of Cx33 after Mse I was used. This enzyme gave two bands for IL-7 alone and IL-7 plus growth factors. Lane 7 is the PCR product of the Cx33 from mouse testis that had been cut with Mse I; two bands were formed. Sequencing the RT-PCR products revealed the presence of mouse Cx43 (99.1% identical) and Cx33 (98.2% identity with rat Cx33 sequence). Similar procedures applied with specific primers for Cx37 ( D , lanes 1, 2 ) and for Cx40 ( E , lanes 1, 2 ) in cells treated with IL-7 alone resulted in detection of a product of a 311 bp, consistent with the expression of Cx40. M , Molecular markers, PCR products of Cx37 (mouse brain; D, lane 1 ) and Cx40 (mouse heart; E, lane 1 ), respectively.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Negative Control, Sequencing, Expressing

5) Product Images from "A Novel Urease-Negative Helicobacter Species Associated with Colitis and Typhlitis in IL-10-Deficient Mice"

Article Title: A Novel Urease-Negative Helicobacter Species Associated with Colitis and Typhlitis in IL-10-Deficient Mice

Journal: Infection and Immunity

doi:

Agarose gel electrophoresis of DNA amplified by PCR with Helicobacter species-specific primers JGF-F1 and JGF-R1 (A) or Helicobacter genus-specific primers C97 and C98 (B). Lanes 1 to 8, novel urease-negative Helicobacter sp. isolates MIT 97-6810 and MIT 97-6811 and isolates 98-6781, 98-6782, 98-784, 98-9785, 98-7686, and 98-6787, respectively; lane 9, H. rodentium 95-1707; lane 10, control with no DNA template; M, 100-bp DNA ladder (GibcoLife Technologies, Gaithersburg, Md.). The 0.6-kb (A) and 0.4-kb (B) positions are indicated by asterisks.
Figure Legend Snippet: Agarose gel electrophoresis of DNA amplified by PCR with Helicobacter species-specific primers JGF-F1 and JGF-R1 (A) or Helicobacter genus-specific primers C97 and C98 (B). Lanes 1 to 8, novel urease-negative Helicobacter sp. isolates MIT 97-6810 and MIT 97-6811 and isolates 98-6781, 98-6782, 98-784, 98-9785, 98-7686, and 98-6787, respectively; lane 9, H. rodentium 95-1707; lane 10, control with no DNA template; M, 100-bp DNA ladder (GibcoLife Technologies, Gaithersburg, Md.). The 0.6-kb (A) and 0.4-kb (B) positions are indicated by asterisks.

Techniques Used: Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction

6) Product Images from "Identification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing"

Article Title: Identification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing

Journal: Molecular Pathology

doi:

DNA products from PCR of 16S rRNA gene. Lane M, molecular marker ( φ X174 HaeIII digest); lane 1, blood culture isolate from patient; lane 2, negative control containing DNase I treated distilled water.
Figure Legend Snippet: DNA products from PCR of 16S rRNA gene. Lane M, molecular marker ( φ X174 HaeIII digest); lane 1, blood culture isolate from patient; lane 2, negative control containing DNase I treated distilled water.

Techniques Used: Polymerase Chain Reaction, Marker, Negative Control

7) Product Images from "Quantitative Analysis of Latent Human Cytomegalovirus"

Article Title: Quantitative Analysis of Latent Human Cytomegalovirus

Journal: Journal of Virology

doi:

CMV DNA copy number determination by QC-PCR. Lysates of 1.3 × 10 4 cells from an RC256-infected GM-P culture (MOI of 3, day 17 postinfection) were each analyzed in the presence of 3 × 10 3 to 3 × 10 5 copies of competitive template, a CMV ie1/ie2 cDNA plasmid pON2347. The copy numbers are indicated above the lanes. The positions of the 387-bp product from CMV genomic DNA and the 217-bp product from the cDNA competitive template are indicated by arrows. Cells from a mock-infected GM-P culture (Mock) or a sample without DNA (no DNA) were included as negative controls. The marker was a 100-bp ladder (Boehringer-Mannheim).
Figure Legend Snippet: CMV DNA copy number determination by QC-PCR. Lysates of 1.3 × 10 4 cells from an RC256-infected GM-P culture (MOI of 3, day 17 postinfection) were each analyzed in the presence of 3 × 10 3 to 3 × 10 5 copies of competitive template, a CMV ie1/ie2 cDNA plasmid pON2347. The copy numbers are indicated above the lanes. The positions of the 387-bp product from CMV genomic DNA and the 217-bp product from the cDNA competitive template are indicated by arrows. Cells from a mock-infected GM-P culture (Mock) or a sample without DNA (no DNA) were included as negative controls. The marker was a 100-bp ladder (Boehringer-Mannheim).

Techniques Used: Polymerase Chain Reaction, Infection, Plasmid Preparation, Marker

8) Product Images from "Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient"

Article Title: Identification by 16S ribosomal RNA gene sequencing of an Enterobacteriaceae species from a bone marrow transplant recipient

Journal: Molecular Pathology

doi:

DNA products from PCR of 16S ribosomal gene. Lane M, molecular marker SPP1 EcoRI digest; lane 1, bacterial isolate from bone marrow transplant recipient; lane 2, negative control containing DNase I treated distilled water.
Figure Legend Snippet: DNA products from PCR of 16S ribosomal gene. Lane M, molecular marker SPP1 EcoRI digest; lane 1, bacterial isolate from bone marrow transplant recipient; lane 2, negative control containing DNase I treated distilled water.

Techniques Used: Polymerase Chain Reaction, Marker, Negative Control

9) Product Images from "Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6"

Article Title: Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi: 10.1073/pnas.062066799

( A ) MLL bcr rearrangements in ALL of patient 45 ( Center ) and panhandle PCR ( Left ) and reverse-panhandle PCR ( Right ) products. The 8.3-kb fragment on Southern blot is from unrearranged MLL allele ( Center , dash); arrows show rearrangements. ( B ) Summary of der(11) genomic breakpoint junction in recombination PCR-generated subclones from panhandle PCR. One subclone was sequenced in its entirety; the breakpoint junction was verified in three more subclones. The 5′ 6,639 bp include MLL primer 4 and MLL bcr sequence. Ninety-six base pairs of 3′ sequence are AF-4 DNA. Seventy-three base pairs of 3′ sequence extend from ligated oligonucleotide (P-Oligo) through MLL primer 3 ( Top ). Arrow shows MLL and AF-4 breakpoint positions ( Bottom ). Underlines show short homologies (bottom). Repetitive sequences are shown ( Middle ). ( C ) Summary of der(4) genomic breakpoint junction in recombination PCR-generated subclone from reverse-panhandle PCR. In reverse-panhandle PCR, nested primer 3 from MLL exon 11/intron 10 anneals to both ends of the template. Thirty-five base pairs of 5′ sequence extend from MLL primer 3 through ligated oligonucleotide (P-Oligo). Twenty-nine to 30 bp of 5′ sequence are AF-4 . The 3′ 2167–2168 bp are MLL bcr sequence through MLL primer 3 ( Top ). Arrowheads show AF-4 and MLL breakpoint positions; “A” residue in both genes precluded precise assignments ( Bottom ). Short homologies are underlined ( Bottom ). Repetitive sequences are shown ( Middle ). One subclone was sequenced in its entirety; three PCRs with gene-specific primers confirmed der(4) breakpoint junction.
Figure Legend Snippet: ( A ) MLL bcr rearrangements in ALL of patient 45 ( Center ) and panhandle PCR ( Left ) and reverse-panhandle PCR ( Right ) products. The 8.3-kb fragment on Southern blot is from unrearranged MLL allele ( Center , dash); arrows show rearrangements. ( B ) Summary of der(11) genomic breakpoint junction in recombination PCR-generated subclones from panhandle PCR. One subclone was sequenced in its entirety; the breakpoint junction was verified in three more subclones. The 5′ 6,639 bp include MLL primer 4 and MLL bcr sequence. Ninety-six base pairs of 3′ sequence are AF-4 DNA. Seventy-three base pairs of 3′ sequence extend from ligated oligonucleotide (P-Oligo) through MLL primer 3 ( Top ). Arrow shows MLL and AF-4 breakpoint positions ( Bottom ). Underlines show short homologies (bottom). Repetitive sequences are shown ( Middle ). ( C ) Summary of der(4) genomic breakpoint junction in recombination PCR-generated subclone from reverse-panhandle PCR. In reverse-panhandle PCR, nested primer 3 from MLL exon 11/intron 10 anneals to both ends of the template. Thirty-five base pairs of 5′ sequence extend from MLL primer 3 through ligated oligonucleotide (P-Oligo). Twenty-nine to 30 bp of 5′ sequence are AF-4 . The 3′ 2167–2168 bp are MLL bcr sequence through MLL primer 3 ( Top ). Arrowheads show AF-4 and MLL breakpoint positions; “A” residue in both genes precluded precise assignments ( Bottom ). Short homologies are underlined ( Bottom ). Repetitive sequences are shown ( Middle ). One subclone was sequenced in its entirety; three PCRs with gene-specific primers confirmed der(4) breakpoint junction.

Techniques Used: Polymerase Chain Reaction, Southern Blot, Generated, Sequencing

10) Product Images from "Protein S is inducible by interleukin 4 in T cells and inhibits lymphoid cell procoagulant activity"

Article Title: Protein S is inducible by interleukin 4 in T cells and inhibits lymphoid cell procoagulant activity

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated RNA samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) PCR confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.
Figure Legend Snippet: Identification of protein S as an IL-4-inducible T cell gene. ( a ) DD of an IL-4-regulated gene fragment. The indicated RNA samples were subjected to DD by using the “10-mer” (5′-AGTTCTTGTG-3′) and “T-mer” (5′-T 12 CT-3′). Open arrows, two genes that are down-regulated upon T cell activation; solid arrow, the IL-4-inducible T cell gene characterized in this report. ( b ) PCR confirmation of the IL-4-inducible T cell gene. RNA was isolated from splenic cells cultured without cytokine (−) or in the presence of IL-4 at 500 units/ml (+) for 24 hr, and cDNA was prepared with (dT) 15 , the “T-mer” 5′-T 12 CT-3′, or random hexamers as indicated. PCR was conducted by using internal primers from the cloned DD product ( Upper ) or from the hypoxanthine phosphoribosyltransferase ( HPRT ) gene ( Lower ). ( c ) Sequence of the IL-4-inducible DD product. The sequence of the portion of a 3-kb clone encoding the DD product. The locations of the “10-mer” and “T-mer” used for DD and the internal primers (IP) used for PCR are indicated. ( d ) are indicated. E, Eco RI; B, Bam HI.

Techniques Used: Activation Assay, Polymerase Chain Reaction, Isolation, Cell Culture, Clone Assay, Sequencing

11) Product Images from "Panhandle PCR strategy to amplify MLL genomic breakpoints in treatment-related leukemias"

Article Title: Panhandle PCR strategy to amplify MLL genomic breakpoints in treatment-related leukemias

Journal: Proceedings of the National Academy of Sciences of the United States of America

doi:

( A ) Panhandle PCR products from der(11) chromosome of t(4;11)(q21;q23) in treatment-related ALL of patient 33. Brackets indicate separate panhandle PCRs where identical 2620-bp products were obtained. ( B ) Sequence of t(4;11) breakpoint junction in individual subclones, 38–1, 38–2, and 38–4, from panhandle PCR. The 5′ 2029 bp include MLL forward nested primer and MLL bcr sequence; 516 bp of 3′ sequence are partner DNA, and 75 bp of 3′ sequence extend from ligated oligonucleotide (P-Oligo) through reverse nested primer. Comparison with normal sequence identified the breakpoint at nucleotide 2158 in MLL intron 6 (bold arrow). Alu J repeats in MLL and partner DNA are shown. Sequences were the same in all three subclones.
Figure Legend Snippet: ( A ) Panhandle PCR products from der(11) chromosome of t(4;11)(q21;q23) in treatment-related ALL of patient 33. Brackets indicate separate panhandle PCRs where identical 2620-bp products were obtained. ( B ) Sequence of t(4;11) breakpoint junction in individual subclones, 38–1, 38–2, and 38–4, from panhandle PCR. The 5′ 2029 bp include MLL forward nested primer and MLL bcr sequence; 516 bp of 3′ sequence are partner DNA, and 75 bp of 3′ sequence extend from ligated oligonucleotide (P-Oligo) through reverse nested primer. Comparison with normal sequence identified the breakpoint at nucleotide 2158 in MLL intron 6 (bold arrow). Alu J repeats in MLL and partner DNA are shown. Sequences were the same in all three subclones.

Techniques Used: Polymerase Chain Reaction, Sequencing

12) Product Images from "Development of Primers to O-Antigen Biosynthesis Genes for Specific Detection of Escherichia coli O157 by PCR"

Article Title: Development of Primers to O-Antigen Biosynthesis Genes for Specific Detection of Escherichia coli O157 by PCR

Journal: Applied and Environmental Microbiology

doi:

Identification of E. coli O157 serotypes by PCR with E. coli O157 rfbB -specific primers. Lane 1, 100-bp ladder (Promega); lane 2, S. enteriditis ; lane 3, S. typhimurium UK1; lane 4, E. coli K-12 LE392 (O16); lane 5, E. coli O11; lane 6, E. coli O18; lane 7, E. coli O26 ATCC 11840; lane 8, E. coli O111; lane 9, E. coli O157:H7; lane 10, E. coli O157:H7; lane 11, E. coli O157:H11; lane 12, E. coli O157:H16; lane 13, E. coli O157:H42; lane 14, E. coli O157:H7; lane 15, E. coli O157:H7; lane 16, E. coli O157:H7; lane 17, E. coli O157:H7; lane 18, C. freundii (O157 + ). DNA fragments were separated on 1.5% agarose–1× TAE gels at 100 V.
Figure Legend Snippet: Identification of E. coli O157 serotypes by PCR with E. coli O157 rfbB -specific primers. Lane 1, 100-bp ladder (Promega); lane 2, S. enteriditis ; lane 3, S. typhimurium UK1; lane 4, E. coli K-12 LE392 (O16); lane 5, E. coli O11; lane 6, E. coli O18; lane 7, E. coli O26 ATCC 11840; lane 8, E. coli O111; lane 9, E. coli O157:H7; lane 10, E. coli O157:H7; lane 11, E. coli O157:H11; lane 12, E. coli O157:H16; lane 13, E. coli O157:H42; lane 14, E. coli O157:H7; lane 15, E. coli O157:H7; lane 16, E. coli O157:H7; lane 17, E. coli O157:H7; lane 18, C. freundii (O157 + ). DNA fragments were separated on 1.5% agarose–1× TAE gels at 100 V.

Techniques Used: Polymerase Chain Reaction

Related Articles

Polymerase Chain Reaction:

Article Title: A Novel Urease-Negative Helicobacter Species Associated with Colitis and Typhlitis in IL-10-Deficient Mice
Article Snippet: .. A 50-μl reaction mixture contained 50 ng of template DNA, 200 μg of bovine serum albumin per ml, 5 pmol of each primer, 1× commercial PCR buffer, and 2.5 U of Taq DNA polymerase (Boehringer Mannheim). ..

Article Title: Contaminations Occurring in Fungal PCR Assays
Article Snippet: .. Detailed and repeated serial analysis of all components of the PCR mixture (nucleotides, primers, water, magnesium chloride, 10× buffer, and Taq polymerase) showed a positive specific band only in the 10× PCR buffer supplied by Boehringer Mannheim. .. Sequencing of the amplicon followed by GenBank sequence homology analysis showed a homology of 99% with DNA of Acremonium spp.

Article Title: Changes in the Properties of Gap Junctions during Neuronal Differentiation of Hippocampal Progenitor Cells
Article Snippet: .. PCR reactions contained 1–2 μg of first-strand cDNA, 50 μ m sense and antisense primers, 8 μl of 10× PCR buffer (in m m : 100 Tris-HCl, 15 MgCl2 , and 500 KCl, pH 8.3) and 2.5 U of Taq polymerase (Boehringer Mannheim) in a final volume of 100 μl. .. The samples underwent 30 cycles of PCR with a PTC-100 thermocycler (M. J.

Article Title: Quantitative Analysis of Latent Human Cytomegalovirus
Article Snippet: .. Cells were harvested and fixed with freshly prepared 4% paraformaldehyde in PBS for 30 min at room temperature, washed three times in PBS, and suspended in a PCR mixture consisting of 200 μM each dATP, dCTP, dGTP, and dTTP, 0.01% gelatin, oligonucleotide primers at 1 pmol/μl each, 1.5 mM MgCl2 , and 1× PCR buffer (Boehringer Mannheim). .. PCR amplification was carried out with the ie1/ie2 primers IEP3A and IEP3B ( ) in a Perkin-Elmer/Cetus thermocycler for 30 cycles (94°C for 2 min, 58°C for 2 min, and 72°C for 5 min) after an initial denaturation at 94°C for 8 min.

Article Title: Identification of slide coagulase positive, tube coagulase negative Staphylococcus aureus by 16S ribosomal RNA gene sequencing
Article Snippet: .. The PCR mixture (50 μl) contained bacterial DNA, PCR buffer (10mM Tris/HCl, pH 8.3, 50mM KCl, 2mM MgCl2 , and 0.01% gelatin), 200μM of each dNTP and 1.0 U Taq polymerase (Boehringer Mannheim, Mannheim, Germany). .. The mixtures were amplified for 40 cycles at 94°C for one minute, 55°C for one minute, and 72°C for two minutes, followed by a final extension at 72°C for 10 minutes in an automated thermal cycler (Perkin-Elmer Cetus, Gouda, the Netherlands).

Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora
Article Snippet: .. Each reaction tube contained approximately 1 μl of a 1-ng/μl DNA template, 5 μl of 10× PCR buffer (Boehringer Mannheim, Indianapolis, Ind.), 36.6 μl of sterile distilled water, 2 μl (each) of 1.25 mM deoxynucleoside triphosphates (Pharmacia Biotech, Piscataway, N.J.), 2 μl of 10 mM MgCl2 (Sigma, St. Louis, Mo.), 2 μl each of 10 μM forward and reverse primers , and 0.4 μl of Taq (5 U/μl; Boehringer Mannheim). ..

Article Title: Gene Expression of Osteoprotegerin Ligand, Osteoprotegerin, and Receptor Activator of NF-?B in Giant Cell Tumor of Bone
Article Snippet: .. Polymerase chain reaction (PCR) was performed using 1.0 U of Taq polymerase (Boehringer Mannheim, Mannheim, Germany) with 0.4 mmol/L of both human and mouse OPGL, OPG, and RANK primers and 0.2 mmol/L of GAPDH and 36B4 primers, 125 μmol/L of dNTP in 1× PCR buffer (Boehringer Mannheim), and water in a total volume of 25 μl. .. The amplification was performed in a DNA thermal cycler (model 2400; Perkin-Elmer) under the following conditions: denaturation at 94°C for 5 minutes for the first cycle and for 30 seconds starting from the second cycle, annealing at 55°C (except for human RANK at 65°C, mouse RANK at 58°C) for 30 seconds, and extension at 72°C for 30 seconds.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Boehringer Mannheim pcr buffer
    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, <t>10×</t> buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the <t>PCR.</t>
    Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr buffer/product/Boehringer Mannheim
    Average 92 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    pcr buffer - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Boehringer Mannheim rt pcr buffer
    <t>RT-PCR</t> and sequence analyses of defective viral RNA. (A) RT-PCR analysis of viral RNA present in either persistently infected Vero cells at P7 (lane 1) or a mouse brain after an intracerebral challenge with MVE virus (lane 2). The oligonucleotide primers K2Ls and <t>3018as</t> were used to amplify the region of the viral genomic RNA from position 1 to 3037. (B) RT-PCR analysis of viral RNA present in two successive passages, P7 and P8, of persistently infected Vero cells (lanes 1 and 2, respectively) by using the oligonucleotide primers K1Ls and 3018as to amplify the region of the viral genome from position 215 to 3037. (C) Diagram showing the sequences of four defective viral RNAs in the vicinity of deletions. The viral genome is depicted at the top. Vertical lines denote the sites of deletions; horizontal lines denote in-frame codons formed by joining nucleotides left of the deletion of the prM- and C-coding units to various nucleotides following the deletion in the NS1-coding unit. The numbers below the sequence denote the positions of codons (amino acids) in the prM-, NS1-, and C-coding units (proteins), respectively.
    Rt Pcr Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rt pcr buffer/product/Boehringer Mannheim
    Average 91 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    rt pcr buffer - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Journal: Journal of Clinical Microbiology

    Article Title: Contaminations Occurring in Fungal PCR Assays

    doi:

    Figure Lengend Snippet: Gel electrophoresis (2% TAE-agarose gel) showing amplified DNA of potentially contaminated samples (specific bands of approximately 500-bp length). Lanes: 1, 100-bp marker; 2, zymolyase, contaminated lot; 3, zymolyase, contaminated lot, 1:10 dilution; 4, 10× buffer from digoxigenin (Dig)-labeling kit (Boehringer Mannheim), contaminated lot; 5, 10× buffer from Dig-labeling kit (Boehringer Mannheim), contaminated lot; 6, zymolyase, regular lot, not contaminated; 7, proteinase K, regular lot, not contaminated; 8, lyticase, contaminated lot; 9, lysing enzyme extracted from T. harzianum ; 10, positive control, A. fumigatus DNA, extracted from 10 3 CFU; 11, negative control of the extraction; and 12, negative control of the PCR.

    Article Snippet: Detailed and repeated serial analysis of all components of the PCR mixture (nucleotides, primers, water, magnesium chloride, 10× buffer, and Taq polymerase) showed a positive specific band only in the 10× PCR buffer supplied by Boehringer Mannheim.

    Techniques: Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Amplification, Marker, Labeling, Positive Control, Negative Control, Polymerase Chain Reaction

    Gene expression of OPGL, OPG, and RANK in GCT. A: Cycle-dependent PCR reactions were removed from 20 to 35 cycles at two- to three-cycle intervals and electrophoresed on 1.5% agarose gels stained with EtBr. The intensity of EtBr fluorescence was measured by densitometry and plotted as a function of cycle number to generate amplification curves for OPGL, OPG, and RANK PCR fragments. B: Expression of OPGL, OPG, and RANK mRNA in samples from five cases of GCT ( lanes 1–5 , cases 1–5, respectively), normal cancellous bone ( lane 6 ), and osteoblast-like osteosarcoma cells U2OS ( lane 7 ). The sizes of OPGL, RANK, OPG, and GAPDH PCR products were 486, 497, 324, and 206 bp, respectively. The GAPDH housekeeping gene determines the variation of loading in the gel.

    Journal: The American Journal of Pathology

    Article Title: Gene Expression of Osteoprotegerin Ligand, Osteoprotegerin, and Receptor Activator of NF-?B in Giant Cell Tumor of Bone

    doi:

    Figure Lengend Snippet: Gene expression of OPGL, OPG, and RANK in GCT. A: Cycle-dependent PCR reactions were removed from 20 to 35 cycles at two- to three-cycle intervals and electrophoresed on 1.5% agarose gels stained with EtBr. The intensity of EtBr fluorescence was measured by densitometry and plotted as a function of cycle number to generate amplification curves for OPGL, OPG, and RANK PCR fragments. B: Expression of OPGL, OPG, and RANK mRNA in samples from five cases of GCT ( lanes 1–5 , cases 1–5, respectively), normal cancellous bone ( lane 6 ), and osteoblast-like osteosarcoma cells U2OS ( lane 7 ). The sizes of OPGL, RANK, OPG, and GAPDH PCR products were 486, 497, 324, and 206 bp, respectively. The GAPDH housekeeping gene determines the variation of loading in the gel.

    Article Snippet: Polymerase chain reaction (PCR) was performed using 1.0 U of Taq polymerase (Boehringer Mannheim, Mannheim, Germany) with 0.4 mmol/L of both human and mouse OPGL, OPG, and RANK primers and 0.2 mmol/L of GAPDH and 36B4 primers, 125 μmol/L of dNTP in 1× PCR buffer (Boehringer Mannheim), and water in a total volume of 25 μl.

    Techniques: Expressing, Polymerase Chain Reaction, Staining, Fluorescence, Amplification

    Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.

    Journal: Applied and Environmental Microbiology

    Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora

    doi:

    Figure Lengend Snippet: Restriction analysis with Rsa I of DNA amplified with primer pair ITS 5 and ITS 4 from P. infestans US-6 NY (lane 2), P. infestans 93-1 (lane 3), P. mirabilis 0S0016 (lane 4), P. fragariae A-8 (lane 5), P. megasperma NY 318 (lane 6), P. megasperma NY 412 (lane 7), P. sojae R1 (lane 8), P. cinnamomi 2302 (lane 9), P. cryptogea PCR-1 (lane 10), and P. erythroseptica 4 (lane 11). Lanes 1 and 12 contain 100-bp ladders.

    Article Snippet: Each reaction tube contained approximately 1 μl of a 1-ng/μl DNA template, 5 μl of 10× PCR buffer (Boehringer Mannheim, Indianapolis, Ind.), 36.6 μl of sterile distilled water, 2 μl (each) of 1.25 mM deoxynucleoside triphosphates (Pharmacia Biotech, Piscataway, N.J.), 2 μl of 10 mM MgCl2 (Sigma, St. Louis, Mo.), 2 μl each of 10 μM forward and reverse primers , and 0.4 μl of Taq (5 U/μl; Boehringer Mannheim).

    Techniques: Amplification, Polymerase Chain Reaction

    Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.

    Journal: Applied and Environmental Microbiology

    Article Title: PCR Amplification of Ribosomal DNA for Species Identification in the Plant Pathogen Genus Phytophthora

    doi:

    Figure Lengend Snippet: Restriction analysis with Hae III of DNA amplified with ITS 5 and ITS 4 from P. palmivora P8 (lane 2), P. erythroseptica 4 (lane 3), P. cryptogea PCR-1 (lane 4), P. citrophthora M86 (lane 5), P. cinnamomi 2302 (lane 6), P. fragariae A-8 (lane 7), P. megasperma NY 412 (lane 8), P. sojae R1 (lane 9), and P. megasperma NY 318 (lane 10). Lanes 1 and 11 contain 100-bp ladders.

    Article Snippet: Each reaction tube contained approximately 1 μl of a 1-ng/μl DNA template, 5 μl of 10× PCR buffer (Boehringer Mannheim, Indianapolis, Ind.), 36.6 μl of sterile distilled water, 2 μl (each) of 1.25 mM deoxynucleoside triphosphates (Pharmacia Biotech, Piscataway, N.J.), 2 μl of 10 mM MgCl2 (Sigma, St. Louis, Mo.), 2 μl each of 10 μM forward and reverse primers , and 0.4 μl of Taq (5 U/μl; Boehringer Mannheim).

    Techniques: Amplification, Polymerase Chain Reaction

    RT-PCR and sequence analyses of defective viral RNA. (A) RT-PCR analysis of viral RNA present in either persistently infected Vero cells at P7 (lane 1) or a mouse brain after an intracerebral challenge with MVE virus (lane 2). The oligonucleotide primers K2Ls and 3018as were used to amplify the region of the viral genomic RNA from position 1 to 3037. (B) RT-PCR analysis of viral RNA present in two successive passages, P7 and P8, of persistently infected Vero cells (lanes 1 and 2, respectively) by using the oligonucleotide primers K1Ls and 3018as to amplify the region of the viral genome from position 215 to 3037. (C) Diagram showing the sequences of four defective viral RNAs in the vicinity of deletions. The viral genome is depicted at the top. Vertical lines denote the sites of deletions; horizontal lines denote in-frame codons formed by joining nucleotides left of the deletion of the prM- and C-coding units to various nucleotides following the deletion in the NS1-coding unit. The numbers below the sequence denote the positions of codons (amino acids) in the prM-, NS1-, and C-coding units (proteins), respectively.

    Journal: Journal of Virology

    Article Title: Characterization of Defective Viral RNA Produced during Persistent Infection of Vero Cells with Murray Valley Encephalitis Virus

    doi:

    Figure Lengend Snippet: RT-PCR and sequence analyses of defective viral RNA. (A) RT-PCR analysis of viral RNA present in either persistently infected Vero cells at P7 (lane 1) or a mouse brain after an intracerebral challenge with MVE virus (lane 2). The oligonucleotide primers K2Ls and 3018as were used to amplify the region of the viral genomic RNA from position 1 to 3037. (B) RT-PCR analysis of viral RNA present in two successive passages, P7 and P8, of persistently infected Vero cells (lanes 1 and 2, respectively) by using the oligonucleotide primers K1Ls and 3018as to amplify the region of the viral genome from position 215 to 3037. (C) Diagram showing the sequences of four defective viral RNAs in the vicinity of deletions. The viral genome is depicted at the top. Vertical lines denote the sites of deletions; horizontal lines denote in-frame codons formed by joining nucleotides left of the deletion of the prM- and C-coding units to various nucleotides following the deletion in the NS1-coding unit. The numbers below the sequence denote the positions of codons (amino acids) in the prM-, NS1-, and C-coding units (proteins), respectively.

    Article Snippet: The RT-PCR mix consisted of 0.2 mM each deoxynucleoside triphosphate, 100 ng of forward primer (see Table ) (primer K2Ls), 200 ng of reverse primer (see Table ) (primer 3018as), 5 mM dithiothreitol, 10 ml of 5× RT-PCR buffer, 1.5 mM MgCl2 , 1 μl of avian myeloblastosis virus, and Expand high-fidelity enzyme blend (Boehringer Mannheim) in a total reaction volume of 50 μl.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Infection