pcr analysis genomic dna  (Millipore)


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    Genomic DNA
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    Millipore pcr analysis genomic dna
    Tamoxifen induced deletion of FAK. A, Genomic <t>DNA</t> of wild‐type (WT) and Cre‐positive floxed FAK mice were amplified by <t>PCR.</t> The WT band is 1.4 kb, the floxed FAK product is 1.6 kb and the postrecombination product is 550 bp. DNA was extracted from cardiac tissue unless otherwise noted. FAK C, Cre‐positive floxed‐FAK mouse not treated with tamoxifen; Sk M, skeletal muscle. B, Representative Western blot showing FAK protein expression in lysates of isolated adult cardiac ventricular myocytes from wild‐type (WT), experimental control (Exp C), and FAK KO mice. C, Normalized integrated intensity data for myocyte FAK expression in WT, Exp C and FAK KO mice. FAK expression in FAK KO mice was reduced by 66.5% compared to WT mice ( P ≤0.001; n=7 hearts, both groups) and by 73% compared to Exp C mice ( P ≤0.001; n=5 hearts [Exp C], n=7 hearts [FAK KO]). Numbers within bars indicate sample size of each group. Exp C indicates experimental control; FAK, focal adhesion kinase; KO, knock out; PCR, polymerase chain reaction.

    https://www.bioz.com/result/pcr analysis genomic dna/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pcr analysis genomic dna - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "Conditional Knockout of Myocyte Focal Adhesion Kinase Abrogates Ischemic Preconditioning in Adult Murine Hearts"

    Article Title: Conditional Knockout of Myocyte Focal Adhesion Kinase Abrogates Ischemic Preconditioning in Adult Murine Hearts

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    doi: 10.1161/JAHA.113.000457

    Tamoxifen induced deletion of FAK. A, Genomic DNA of wild‐type (WT) and Cre‐positive floxed FAK mice were amplified by PCR. The WT band is 1.4 kb, the floxed FAK product is 1.6 kb and the postrecombination product is 550 bp. DNA was extracted from cardiac tissue unless otherwise noted. FAK C, Cre‐positive floxed‐FAK mouse not treated with tamoxifen; Sk M, skeletal muscle. B, Representative Western blot showing FAK protein expression in lysates of isolated adult cardiac ventricular myocytes from wild‐type (WT), experimental control (Exp C), and FAK KO mice. C, Normalized integrated intensity data for myocyte FAK expression in WT, Exp C and FAK KO mice. FAK expression in FAK KO mice was reduced by 66.5% compared to WT mice ( P ≤0.001; n=7 hearts, both groups) and by 73% compared to Exp C mice ( P ≤0.001; n=5 hearts [Exp C], n=7 hearts [FAK KO]). Numbers within bars indicate sample size of each group. Exp C indicates experimental control; FAK, focal adhesion kinase; KO, knock out; PCR, polymerase chain reaction.
    Figure Legend Snippet: Tamoxifen induced deletion of FAK. A, Genomic DNA of wild‐type (WT) and Cre‐positive floxed FAK mice were amplified by PCR. The WT band is 1.4 kb, the floxed FAK product is 1.6 kb and the postrecombination product is 550 bp. DNA was extracted from cardiac tissue unless otherwise noted. FAK C, Cre‐positive floxed‐FAK mouse not treated with tamoxifen; Sk M, skeletal muscle. B, Representative Western blot showing FAK protein expression in lysates of isolated adult cardiac ventricular myocytes from wild‐type (WT), experimental control (Exp C), and FAK KO mice. C, Normalized integrated intensity data for myocyte FAK expression in WT, Exp C and FAK KO mice. FAK expression in FAK KO mice was reduced by 66.5% compared to WT mice ( P ≤0.001; n=7 hearts, both groups) and by 73% compared to Exp C mice ( P ≤0.001; n=5 hearts [Exp C], n=7 hearts [FAK KO]). Numbers within bars indicate sample size of each group. Exp C indicates experimental control; FAK, focal adhesion kinase; KO, knock out; PCR, polymerase chain reaction.

    Techniques Used: Mouse Assay, Amplification, Polymerase Chain Reaction, Western Blot, Expressing, Isolation, Knock-Out

    2) Product Images from "Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera"

    Article Title: Agrobacterium-Mediated Stable Genetic Transformation of Populus angustifolia and Populus balsamifera

    Journal: Frontiers in Plant Science

    doi: 10.3389/fpls.2016.00296

    Polymerase chain reaction (PCR) and Southern blot analyses for checking the presence of a transgene. (A) PCR analysis. LucF and LucR primers were used to amplify a 575 bp fragment from the luc region indicating the presence of a transgene. P – a positive control (pCAMBIA:: NLUC plasmid); M – Generuler 1 kb ladder (Thermo Fisher Scientific); 1–6: six independent putative transformants of P. angustifolia; 7–11: five independent putative transformants of P. balsamifera; WT- a negative control (a wild-type plant); NTC – no template control; 575 bp – amplicon size. (B) Southern blot analysis of putative transformants by using a DIG labeled probe complimentary to the luciferase transgene. Design of the 13470 bp long pCAMBIA-Npro-long-Luc vector containing the T-DNA ( Boyko et al., 2011 ). Genomic DNA of putative transformants expressing luciferase and positive for the presence of a 575 bp fragment from the luc region in the PCR assay was digested with the restriction enzyme Nde I which cuts the T-DNA at a single site. The number of fragments for each sample on the blot corresponds to the number of loci carrying the transgene. (C) Picture of Southern blot analysis. WT- A wild-type negative control plant; M – DIG labeled DNA molecular weight marker (Sigma-Aldrich); 1–3: 10, 100, and 250 pg of plasmid, respectively, used as a positive control; 4,5: two independent putative transformants obtained from intermodal explants of P. angustifolia; 6,7: two independent putative transformants obtained from axillary bud explants of P. angustifolia; 8,9: two independent putative transformants obtained from axillary bud explants of P. balsamifera ; 10,11: two independent putative transformants obtained from internodal explants of P. balsamifera .
    Figure Legend Snippet: Polymerase chain reaction (PCR) and Southern blot analyses for checking the presence of a transgene. (A) PCR analysis. LucF and LucR primers were used to amplify a 575 bp fragment from the luc region indicating the presence of a transgene. P – a positive control (pCAMBIA:: NLUC plasmid); M – Generuler 1 kb ladder (Thermo Fisher Scientific); 1–6: six independent putative transformants of P. angustifolia; 7–11: five independent putative transformants of P. balsamifera; WT- a negative control (a wild-type plant); NTC – no template control; 575 bp – amplicon size. (B) Southern blot analysis of putative transformants by using a DIG labeled probe complimentary to the luciferase transgene. Design of the 13470 bp long pCAMBIA-Npro-long-Luc vector containing the T-DNA ( Boyko et al., 2011 ). Genomic DNA of putative transformants expressing luciferase and positive for the presence of a 575 bp fragment from the luc region in the PCR assay was digested with the restriction enzyme Nde I which cuts the T-DNA at a single site. The number of fragments for each sample on the blot corresponds to the number of loci carrying the transgene. (C) Picture of Southern blot analysis. WT- A wild-type negative control plant; M – DIG labeled DNA molecular weight marker (Sigma-Aldrich); 1–3: 10, 100, and 250 pg of plasmid, respectively, used as a positive control; 4,5: two independent putative transformants obtained from intermodal explants of P. angustifolia; 6,7: two independent putative transformants obtained from axillary bud explants of P. angustifolia; 8,9: two independent putative transformants obtained from axillary bud explants of P. balsamifera ; 10,11: two independent putative transformants obtained from internodal explants of P. balsamifera .

    Techniques Used: Polymerase Chain Reaction, Southern Blot, Positive Control, Plasmid Preparation, Negative Control, Amplification, Labeling, Luciferase, Expressing, Molecular Weight, Marker

    3) Product Images from "Specific targeting of cytosine methylation to DNA sequences in vivo"

    Article Title: Specific targeting of cytosine methylation to DNA sequences in vivo

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkl1053

    ( A ) Schematic diagram describing mammalian vectors used for the expression of targeted Mtases. ( B ) Upper panel showing western blot confirming expression levels of 4aFH, 4bFH and 4aQC targeted Mtase enzymes from vector described in ( A ). Lower panel showing the results of RT–PCR of 4aZfFH RNA at 3, 6 and 12 days post-transfection with 4aZfFH targeted Mtase expression vector. β-Actin was used an internal control. PCR was performed for 28, 30, 32 and 34 cycles. ( C ) Bisulphite sequencing results for genomic target site DNA, 14 days post-transient transfection with targeted Mtase or control vector. The target site sequence is given at the top of the figure. HpaII sites are in boldface and the 12 bp zinc-finger recognition site is boxed. Below this is a schematic diagram showing target site and flanking CpG site distribution (represented by circles in sequencing data) spanning ∼160 bp. Large ticks indicate HpaII sites, small ticks indicate HhaI sites. Zinc-finger recognition sites for the 4aZf protein are represented by filled rectangles. The expression vector transiently transfected in each experiment is indicated next to each sequencing results block. Filled circles indicate observed methylation.
    Figure Legend Snippet: ( A ) Schematic diagram describing mammalian vectors used for the expression of targeted Mtases. ( B ) Upper panel showing western blot confirming expression levels of 4aFH, 4bFH and 4aQC targeted Mtase enzymes from vector described in ( A ). Lower panel showing the results of RT–PCR of 4aZfFH RNA at 3, 6 and 12 days post-transfection with 4aZfFH targeted Mtase expression vector. β-Actin was used an internal control. PCR was performed for 28, 30, 32 and 34 cycles. ( C ) Bisulphite sequencing results for genomic target site DNA, 14 days post-transient transfection with targeted Mtase or control vector. The target site sequence is given at the top of the figure. HpaII sites are in boldface and the 12 bp zinc-finger recognition site is boxed. Below this is a schematic diagram showing target site and flanking CpG site distribution (represented by circles in sequencing data) spanning ∼160 bp. Large ticks indicate HpaII sites, small ticks indicate HhaI sites. Zinc-finger recognition sites for the 4aZf protein are represented by filled rectangles. The expression vector transiently transfected in each experiment is indicated next to each sequencing results block. Filled circles indicate observed methylation.

    Techniques Used: Expressing, Western Blot, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Transfection, Polymerase Chain Reaction, Bisulfite Sequencing, Sequencing, Blocking Assay, Methylation

    Analysis of genomic DNA from bacteria expressing targeted methyltransferase enzymes. ( A ) Restriction analysis of genomic DNA recovered from bacteria expressing various 4aHhaI enzyme derivatives (and containing the target site vector), described above the relevant lanes. The lane denoted ‘Con’ represents genomic DNA isolated from untransformed bacteria. U, uncut; C, cut with HhaI restriction enzyme. (Fragments seen in some ‘cut’ lanes are derived from plasmid carryover during genomic DNA preparation, shown using arrows. Input genomic DNA is shown using asterisks.) ( B ) Arbitrary primed PCR of the HhaI restricted genomic DNA described in ( A ). ( C ) Restriction analysis of genomic DNA recovered from bacteria expressing various 4aHpaII enzyme derivatives, described above the relevant lanes. pWt is plasmid DNA coding for the wild-type targeted enzyme. U, uncut, C, cut with HpaII. ( D ) Arbitrary primed PCR of HpaII restricted genomic DNA described in ( C ). M is 1 kb ladder (NEB), 100bp is 100 bp ladder (NEB).
    Figure Legend Snippet: Analysis of genomic DNA from bacteria expressing targeted methyltransferase enzymes. ( A ) Restriction analysis of genomic DNA recovered from bacteria expressing various 4aHhaI enzyme derivatives (and containing the target site vector), described above the relevant lanes. The lane denoted ‘Con’ represents genomic DNA isolated from untransformed bacteria. U, uncut; C, cut with HhaI restriction enzyme. (Fragments seen in some ‘cut’ lanes are derived from plasmid carryover during genomic DNA preparation, shown using arrows. Input genomic DNA is shown using asterisks.) ( B ) Arbitrary primed PCR of the HhaI restricted genomic DNA described in ( A ). ( C ) Restriction analysis of genomic DNA recovered from bacteria expressing various 4aHpaII enzyme derivatives, described above the relevant lanes. pWt is plasmid DNA coding for the wild-type targeted enzyme. U, uncut, C, cut with HpaII. ( D ) Arbitrary primed PCR of HpaII restricted genomic DNA described in ( C ). M is 1 kb ladder (NEB), 100bp is 100 bp ladder (NEB).

    Techniques Used: Expressing, Plasmid Preparation, Isolation, Derivative Assay, Polymerase Chain Reaction

    Related Articles

    Clone Assay:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Amplification:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Agarose Gel Electrophoresis:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Expressing:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Southern Blot:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Methylation:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Isolation:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Purification:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Mouse Assay:

    Article Title: A NOVEL, DIRECT NO DONOR REGULATES OSTEOBLAST AND OSTEOCLAST FUNCTIONS AND INCREASES BONE MASS IN OVARIECTOMIZED MICE
    Article Snippet: .. To generate PRKG2f/f mice, we PCR-amplified genomic DNA fragments encoding prkg2 exon III with flanking sequences from 129/SvJ ES cells using KOD polymerase (EMD Millipore Corporation). .. The prkg2 -floxed construct was assembled in the vector pDLNL (gift from Ju Chen of UCSD) and consisted of a 4.2 kb 5′ arm, a 0.55 kb fragment including exon III flanked by LoxP sites, a 1.3 kb neo cassette flanked by FRT sites, and a 3kb 3′ arm.

    Polymerase Chain Reaction:

    Article Title: A NOVEL, DIRECT NO DONOR REGULATES OSTEOBLAST AND OSTEOCLAST FUNCTIONS AND INCREASES BONE MASS IN OVARIECTOMIZED MICE
    Article Snippet: .. To generate PRKG2f/f mice, we PCR-amplified genomic DNA fragments encoding prkg2 exon III with flanking sequences from 129/SvJ ES cells using KOD polymerase (EMD Millipore Corporation). .. The prkg2 -floxed construct was assembled in the vector pDLNL (gift from Ju Chen of UCSD) and consisted of a 4.2 kb 5′ arm, a 0.55 kb fragment including exon III flanked by LoxP sites, a 1.3 kb neo cassette flanked by FRT sites, and a 3kb 3′ arm.

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Article Title: A refined Panax ginseng karyotype based on an ultra-high copy 167-bp tandem repeat and ribosomal DNAs
    Article Snippet: .. The PCR mixture (total volume of 50 μL) consisted of 2.5 U ExTaq DNA polymerase, 5 μL 10× ExTaq buffer, 2.5mM each dNTP (RR001A; TaKaRa Bio Inc., Kusatsu, Shiga, Japan), 0.3μM each primer, < 500 ng BAC PgH005J07 or P. ginseng gDNA template, and DNase-free water (W4502; Sigma–Aldrich, St. Louis, MO, USA). ..

    Incubation:

    Article Title: Cleavage of Treponema denticola PrcA Polypeptide To Yield Protease Complex-Associated Proteins Prca1 and Prca2 Is Dependent on PrtP
    Article Snippet: .. For Southern blot analysis, Hin dIII-digested genomic DNAs separated on 0.7% agarose gel were transferred to nylon membranes (Immobilon-Ny; Millipore) and hybridized with biotin-labeled DNA probes, followed by incubation of the blots with streptavidin, biotinylated alkaline phosphatase, and chemiluminescence detection reagent (New England Biolabs) according to the manufacturer's instructions. .. Chemiluminescence was detected with a Fluor-S Multi-Imager (Bio-Rad).

    Positron Emission Tomography:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    BAC Assay:

    Article Title: A refined Panax ginseng karyotype based on an ultra-high copy 167-bp tandem repeat and ribosomal DNAs
    Article Snippet: .. The PCR mixture (total volume of 50 μL) consisted of 2.5 U ExTaq DNA polymerase, 5 μL 10× ExTaq buffer, 2.5mM each dNTP (RR001A; TaKaRa Bio Inc., Kusatsu, Shiga, Japan), 0.3μM each primer, < 500 ng BAC PgH005J07 or P. ginseng gDNA template, and DNase-free water (W4502; Sigma–Aldrich, St. Louis, MO, USA). ..

    Modification:

    Article Title: Epigenetic Downregulation and Growth Inhibition of IGFBP7 in Gastric Cancer
    Article Snippet: .. Methylation specific-PCR (MSP) Genomic DNA was isolated by Chelex-100 (Sigma Aldrich) and modified using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). .. Bisulfite-modified DNA was amplified using specific primer sets that discriminate between unmethylated and methylated promoter regions of IGFBP7.

    Transformation Assay:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Recombinant:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

    Plasmid Preparation:

    Article Title: SslE Elicits Functional Antibodies That Impair In Vitro Mucinase Activity and In Vivo Colonization by Both Intestinal and Extraintestinal Escherichia coli Strains
    Article Snippet: .. Cloning, expression and purification of SslE recombinant protein The sslE gene was amplified by PCR from the IHE3034 genomic DNA template, cloned into the pET-21b vector (Novagen) and transformed into DH5α-T1R chemically competent cells for propagation. .. BL21(DE3) chemically competent cells were used for His-tagged protein expression.

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  • 99
    Millipore methylation specific pcr analysis genomic dna
    <t>MS-PCR</t> analysis of SNRPN gene in PWS/ AS patients. MS-PCR products of 174 bp and 100 bp are amplified from methylated and unmethylated alleles of the SNRPN gene locus respectively. a Lane 1, 1000-bp <t>DNA</t> ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, PWS patient from case 2; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control. b Lane 1, 1000-bp DNA ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, AS patient from case 38; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control
    Methylation Specific Pcr Analysis Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/methylation specific pcr analysis genomic dna/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    methylation specific pcr analysis genomic dna - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    95
    Millipore human male genomic dna
    Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic <t>DNA</t> and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific <t>PCR</t> and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'
    Human Male Genomic Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human male genomic dna/product/Millipore
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human male genomic dna - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    Image Search Results


    MS-PCR analysis of SNRPN gene in PWS/ AS patients. MS-PCR products of 174 bp and 100 bp are amplified from methylated and unmethylated alleles of the SNRPN gene locus respectively. a Lane 1, 1000-bp DNA ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, PWS patient from case 2; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control. b Lane 1, 1000-bp DNA ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, AS patient from case 38; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control

    Journal: Molecular Cytogenetics

    Article Title: Genetic testing for Prader-Willi syndrome and Angelman syndrome in the clinical practice of Guangdong Province, China

    doi: 10.1186/s13039-019-0420-x

    Figure Lengend Snippet: MS-PCR analysis of SNRPN gene in PWS/ AS patients. MS-PCR products of 174 bp and 100 bp are amplified from methylated and unmethylated alleles of the SNRPN gene locus respectively. a Lane 1, 1000-bp DNA ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, PWS patient from case 2; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control. b Lane 1, 1000-bp DNA ladder marker; Lane 2, patient’s father; Lane 3, patient’s mother; Lane 4 and 5, AS patient from case 38; Lane 6, negative control; Lane 7, PWS positive control; Lane 8, AS positive control; Lane 9, blank control

    Article Snippet: Methylation-specific PCR analysis Genomic DNA was treated with sulfite using the CpGenome Turbo Bisulfite Modification Kit (Millipore, CA, USA) according to the manufacturer’s instructions.

    Techniques: Mass Spectrometry, Polymerase Chain Reaction, Amplification, Methylation, Marker, Negative Control, Positive Control

    Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic DNA and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific PCR and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'

    Journal: Oncogene

    Article Title: Overexpression of ribosomal RNA in prostate cancer is common but not linked to rDNA promoter hypomethylation

    doi: 10.1038/onc.2011.319

    Figure Lengend Snippet: Methylation status of the rRNA promoter in the parental colorectal carcinoma cells HCT116 and its derivative knockout cell lines. The methylation status of each CpG dinucleotide spanning −158 to +29 bp of human rRNA promoter in HCT116 colorectal carcinoma cells and derivative lines with targeted disruption of DNMT1 , DNMT3b or both genes. The rRNA promoter region was amplified from bisulfite-treated genomic DNA and cloned. The 16–19 randomly selected clones from each sample were subjected to automated sequencing. Each row represents the sequence of an individual clone, whereas each column depicts the position of the CpG. The filled and open circles denote methylated and unmethylated CpGs, respectively. The top row shows a heat map of methylated CpGs representing the frequency of methylation in each of the individual clones shown below. The degree of hypermethylation (NIM) at three CpG islands was assessed using quantitative real-time methylation-specific PCR and was normalized to the amount of bisulfite-converted input as described in ‘Materials and methods.'

    Article Snippet: PCR, genotyping and bisulfite genomic sequencing For initial PCR genotyping of the rDNA locus, 1 μl of human male genomic DNA (EMD Chemicals, Gibbstown, NJ, USA) was amplified by PCR using platinum Taq DNA polymerase (Invitrogen).

    Techniques: Methylation, Knock-Out, Amplification, Clone Assay, Sequencing, Polymerase Chain Reaction

    The human rDNA promoter region. ( a ) Genotyping of rDNA promoter region. The sequence of the rDNA promoter as shown was identical for all prostate cancer tissues and normal tissues, as well as all the cell lines. In this map, primer sequences for genotyping are indicated by the underlined regions and the primer sequences for bisulfite sequencing are indicated as dotted underlines. CpG dinucleotides are boxed with the location marked relative to the transcription start site (arrow). Discrepancies with the published DNA sequence (U13369) are marked. Nucleotides that show base substitutions as compared with the reference U13369 seqeunce are illustrated with dots above the DNA sequence. Deletions (generally a single base), relative to the reference sequence, are marked by a ‘v ‘between nucleotides. ( b ) Schematic representation of a single human rDNA repeat. The approximate positions relative to the transcription start site are indicated (DNA base number in kb). ( c ) Vertical lines indicate locations of CpG dinucleotides on the 45S rRNA promoter relative to the transcription start site, indicated with the dashed curved arrow, with primer pairs used for bisulfite mapping marked by inward pointing arrows.

    Journal: Oncogene

    Article Title: Overexpression of ribosomal RNA in prostate cancer is common but not linked to rDNA promoter hypomethylation

    doi: 10.1038/onc.2011.319

    Figure Lengend Snippet: The human rDNA promoter region. ( a ) Genotyping of rDNA promoter region. The sequence of the rDNA promoter as shown was identical for all prostate cancer tissues and normal tissues, as well as all the cell lines. In this map, primer sequences for genotyping are indicated by the underlined regions and the primer sequences for bisulfite sequencing are indicated as dotted underlines. CpG dinucleotides are boxed with the location marked relative to the transcription start site (arrow). Discrepancies with the published DNA sequence (U13369) are marked. Nucleotides that show base substitutions as compared with the reference U13369 seqeunce are illustrated with dots above the DNA sequence. Deletions (generally a single base), relative to the reference sequence, are marked by a ‘v ‘between nucleotides. ( b ) Schematic representation of a single human rDNA repeat. The approximate positions relative to the transcription start site are indicated (DNA base number in kb). ( c ) Vertical lines indicate locations of CpG dinucleotides on the 45S rRNA promoter relative to the transcription start site, indicated with the dashed curved arrow, with primer pairs used for bisulfite mapping marked by inward pointing arrows.

    Article Snippet: PCR, genotyping and bisulfite genomic sequencing For initial PCR genotyping of the rDNA locus, 1 μl of human male genomic DNA (EMD Chemicals, Gibbstown, NJ, USA) was amplified by PCR using platinum Taq DNA polymerase (Invitrogen).

    Techniques: Sequencing, Methylation Sequencing