pcna positive cells  (Thermo Fisher)


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  • 99
    Name:
    PCNA Proliferating Cell Nuclear Antigen
    Description:

    Catalog Number:
    rb-9055-pcl
    Price:
    None
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    Structured Review

    Thermo Fisher pcna positive cells

    https://www.bioz.com/result/pcna positive cells/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcna positive cells - by Bioz Stars, 2020-09
    99/100 stars

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    Immunohistochemistry:

    Article Title: A Comparative Analysis of the Osteogenic Effects of BMP-2, FGF-2, and VEGFA in a Calvarial Defect Model
    Article Snippet: .. For proliferating cell nuclear antigen (PCNA), immunohistochemistry was performed using a commercially available kit (#93-1143; Invitrogen) according to the manufacturer's instruction. .. Tartrate resistant acid phosphatase (TRAP) staining was performed with the Leukocyte Acid Phosphatase Kit and Fast Red Violet (both Sigma Aldrich) as previously described.

    Recombinase Polymerase Amplification:

    Article Title: Replication initiation complex formation in the absence of nuclear function in Xenopus
    Article Snippet: .. Antibodies Antibodies used were: polyclonals: XcyclinE (gift from C. Bonne-Andrea); XCdc45 (gift from H. Takisawa); RCC1, XLamin B3 (gift from N. Morin); histone H3 and phospho-histone H2A.X (Cell Signaling); phospho MCM2 (S108) (Bethyl Laboratories); phospho ATM/R substrate (Cell Signaling); geminin, Cdt1, Cdc6, RPA, (gifts from Marcel Méchali); monoclonals: MCM7, PCNA, pol ε (Labvision); PSTAIR (Sigma); phospho-ATM (S1981) (Rockland). ..

    Expressing:

    Article Title: Critical illness induces nutrient-independent adipogenesis and accumulation of alternatively activated tissue macrophages
    Article Snippet: .. For the quantification of Proliferating Cell Nuclear Antigen (PCNA), CCAAT/enhancer binding protein-β (C/EBPβ), hormone sensitive lipase (HSL), tumor necrosis factor-α (TNFα), inducible nitric oxide synthase (iNOS), Arginase-1, interleukin 10 (IL-10), chemokine (C-C motif) ligand 2 (CCL2), and CD-163 messenger RNA, commercial gene expression assays (TaqMan, Life Technologies, Gent, Belgium) were used. ..

    Article Title: NBCe1 in Mouse and Human Ameloblasts may be Indirectly Regulated by Fluoride
    Article Snippet: .. The expression of human Proliferating Cell Nuclear Antigen (PCNA) , pituitary homeobox 2 (Pitx2) , amelogenin, and both human and mouse SLC4A4 was examined by quantitative real-time PCR in an ABI 7500 system (Applied Biosystems, Foster City, CA, USA). mRNA expression was compared by ΔΔCt ( ) (see Appendix for detailed explanation of ΔΔCt analysis). ..

    Quantitative RT-PCR:

    Article Title: Long non-coding RNA MALAT1 suppresses the proliferation and migration of endothelial progenitor cells in deep vein thrombosis by regulating the Wnt/β-catenin pathway
    Article Snippet: .. RT-qPCR RT-qPCR was performed to evaluate the levels of MALAT1, proliferating cell nuclear antigen (PCNA), segment polarity protein dishevelled homolog DVL-2 (DVL2), GSK3β, cyclin D1 and β-catenin. .. Total RNA from clinical samples or cells was extracted using TRIzol® reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol.

    Real-time Polymerase Chain Reaction:

    Article Title: NBCe1 in Mouse and Human Ameloblasts may be Indirectly Regulated by Fluoride
    Article Snippet: .. The expression of human Proliferating Cell Nuclear Antigen (PCNA) , pituitary homeobox 2 (Pitx2) , amelogenin, and both human and mouse SLC4A4 was examined by quantitative real-time PCR in an ABI 7500 system (Applied Biosystems, Foster City, CA, USA). mRNA expression was compared by ΔΔCt ( ) (see Appendix for detailed explanation of ΔΔCt analysis). ..

    Mass Spectrometry:

    Article Title: Cataloging antineoplastic agents according to their effectiveness against platinum-resistant and platinum-sensitive ovarian carcinoma cell lines
    Article Snippet: .. Antibodies to the following proteins were purchased: p53 (sc-6243, Santa Cruz Biotechnology, Santa Cruz, CA); BRCA2 (MAB2476, R & D Systems, Minneapolis, MN); Rad51 (MS-988, clone 3C10, Thermo Scientific, Fremont, CA); RPA70 (MS-692, Thermo Scientific), PCNA (MS-106, clone PC10, Thermo Scientific); γH2AX (sc-101696, Santa Cruz); deoxycytidine kinase (MABC188, clone 9D4, EMD Millipore, Temecula, CA); O 6 -methylguanine-DNA methyltransferase (MAB16200, clone MT 3.1, EMD Millipore). ..

    Binding Assay:

    Article Title: Critical illness induces nutrient-independent adipogenesis and accumulation of alternatively activated tissue macrophages
    Article Snippet: .. For the quantification of Proliferating Cell Nuclear Antigen (PCNA), CCAAT/enhancer binding protein-β (C/EBPβ), hormone sensitive lipase (HSL), tumor necrosis factor-α (TNFα), inducible nitric oxide synthase (iNOS), Arginase-1, interleukin 10 (IL-10), chemokine (C-C motif) ligand 2 (CCL2), and CD-163 messenger RNA, commercial gene expression assays (TaqMan, Life Technologies, Gent, Belgium) were used. ..

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  • 95
    Thermo Fisher mouse anti pcna
    Self-renewal of neural progenitors in cerebellum was not abrogated upon Sox9 inactivation as revealed by immunofluorescence staining on cerebellar sagittal sections. (A-D) Expression of the neural stem cell marker Sox2 was not lost in the Sox9- null cerebellum during late gestation under both En1-Cre and <t>Pax2-Cre</t> mediated Sox9 inactivation. In both groups, scattered signals were observed in the VZ and the prospective white matter, while the EGL displayed the strongest signal which corresponded to the actively proliferating granule cell precursors. (A’) and (B’) showed enlarged view of the VZ. (E-J) Quantification of mitotic activity at the VZ by double pulses of BrdU at either E16.5 (E-F) or E18.5 (G-H) did not reveal any difference in the proliferation of VZ neural progenitors between control and the Pax2-Cre induced Sox9 -null mutant. The number of BrdU-labeled cells in the VZ was divided by the total number of VZ cells to obtain the labeling index (I-J) . No statistically significant difference was observed using the Student’s t -test at both E16.5 (I; p = 0.4906) and E18.5 (J; p = 0.1633). (K-R) In addition, we did not observe any alterations in the expression of proliferation associated markers <t>PCNA</t> and Ki67 upon Sox9 inactivation. (A’-Q’) are the enlarged view at the VZ of (A-Q) . The error bars indicate standard deviations. Abbreviations: egl, external granular layer; pcl, Purkinje cell layer; rl, rhombic lip; vz, ventricular zone. Scale bars: A-S : 100 μm; A’-B’, G’-H’, K’-L’, P’-Q’: 50 μm.
    Mouse Anti Pcna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pcna/product/Thermo Fisher
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mouse anti pcna - by Bioz Stars, 2020-09
    95/100 stars
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    Self-renewal of neural progenitors in cerebellum was not abrogated upon Sox9 inactivation as revealed by immunofluorescence staining on cerebellar sagittal sections. (A-D) Expression of the neural stem cell marker Sox2 was not lost in the Sox9- null cerebellum during late gestation under both En1-Cre and Pax2-Cre mediated Sox9 inactivation. In both groups, scattered signals were observed in the VZ and the prospective white matter, while the EGL displayed the strongest signal which corresponded to the actively proliferating granule cell precursors. (A’) and (B’) showed enlarged view of the VZ. (E-J) Quantification of mitotic activity at the VZ by double pulses of BrdU at either E16.5 (E-F) or E18.5 (G-H) did not reveal any difference in the proliferation of VZ neural progenitors between control and the Pax2-Cre induced Sox9 -null mutant. The number of BrdU-labeled cells in the VZ was divided by the total number of VZ cells to obtain the labeling index (I-J) . No statistically significant difference was observed using the Student’s t -test at both E16.5 (I; p = 0.4906) and E18.5 (J; p = 0.1633). (K-R) In addition, we did not observe any alterations in the expression of proliferation associated markers PCNA and Ki67 upon Sox9 inactivation. (A’-Q’) are the enlarged view at the VZ of (A-Q) . The error bars indicate standard deviations. Abbreviations: egl, external granular layer; pcl, Purkinje cell layer; rl, rhombic lip; vz, ventricular zone. Scale bars: A-S : 100 μm; A’-B’, G’-H’, K’-L’, P’-Q’: 50 μm.

    Journal: Molecular Brain

    Article Title: Sox9 is critical for suppression of neurogenesis but not initiation of gliogenesis in the cerebellum

    doi: 10.1186/s13041-015-0115-0

    Figure Lengend Snippet: Self-renewal of neural progenitors in cerebellum was not abrogated upon Sox9 inactivation as revealed by immunofluorescence staining on cerebellar sagittal sections. (A-D) Expression of the neural stem cell marker Sox2 was not lost in the Sox9- null cerebellum during late gestation under both En1-Cre and Pax2-Cre mediated Sox9 inactivation. In both groups, scattered signals were observed in the VZ and the prospective white matter, while the EGL displayed the strongest signal which corresponded to the actively proliferating granule cell precursors. (A’) and (B’) showed enlarged view of the VZ. (E-J) Quantification of mitotic activity at the VZ by double pulses of BrdU at either E16.5 (E-F) or E18.5 (G-H) did not reveal any difference in the proliferation of VZ neural progenitors between control and the Pax2-Cre induced Sox9 -null mutant. The number of BrdU-labeled cells in the VZ was divided by the total number of VZ cells to obtain the labeling index (I-J) . No statistically significant difference was observed using the Student’s t -test at both E16.5 (I; p = 0.4906) and E18.5 (J; p = 0.1633). (K-R) In addition, we did not observe any alterations in the expression of proliferation associated markers PCNA and Ki67 upon Sox9 inactivation. (A’-Q’) are the enlarged view at the VZ of (A-Q) . The error bars indicate standard deviations. Abbreviations: egl, external granular layer; pcl, Purkinje cell layer; rl, rhombic lip; vz, ventricular zone. Scale bars: A-S : 100 μm; A’-B’, G’-H’, K’-L’, P’-Q’: 50 μm.

    Article Snippet: Antibodies Primary antibodies and dilutions used were as follows: rabbit anti-BLBP (1:500, Millipore ABN14); rat anti-BrdU (1:70, Abcam ab6326); rabbit anti-Calbindin D-28K (1:250, Millipore AB1178); rabbit anti-cleaved caspase-3 (1:200, Cell Signaling Technology 9664); rabbit anti-EAAT1 (GLAST; 1:250, Abcam ab416); rabbit anti-Foxp2 (1:1000, Abcam ab16046); rabbit anti-Ki67 (1:100; Millipore AB9260), rabbit anti-Lim-1 (Lhx1; 1:100, Millipore AB3200); rabbit anti-Pax2 (1:500, Invitrogen 71–6000); rabbit anti-Pax6 (1:1000, Millipore AB2237); mouse anti-PCNA (1:200, Thermo Scientific MA5-11358); rabbit anti-Sox2 (1:200, Millipore AB5603); rabbit anti-Sox9 (1:2000, Millipore AB5535); goat anti-hSox9 (1:200, R & D Systems AF3075); goat anti-Sox10 (1:50, Santa Cruz sc-17342).

    Techniques: Immunofluorescence, Staining, Expressing, Marker, Activity Assay, Mutagenesis, Labeling