pcmv3 flag thbs1 plasmid  (Sino Biological)


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    Structured Review

    Sino Biological pcmv3 flag thbs1 plasmid
    The steady-state level of <t>THBS1</t> protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).
    Pcmv3 Flag Thbs1 Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv3 flag thbs1 plasmid/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 flag thbs1 plasmid - by Bioz Stars, 2021-07
    86/100 stars

    Images

    1) Product Images from "Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells"

    Article Title: Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells

    Journal: Cell Cycle

    doi: 10.1080/15384101.2020.1754584

    The steady-state level of THBS1 protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).
    Figure Legend Snippet: The steady-state level of THBS1 protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).

    Techniques Used: Inhibition, Immunofluorescence

    Knockdown of THBS1 sensitizes cells to CB-5083 and promotes apoptosis. ( A ) HCT116 cells transfected with siControl or siTHBS1-1 (shortly siTHBS1) were treated with or without 0.75 μM CB-5083 for 8 h. Total extracts were analyzed by immunoblotting. Actin was used as a loading control. ( B ) HCT116 cells transfected with siControl or siTHBS1 were treated with or without 0.375 μM CB-5083 for 72 h. Cells stained with crystal violet and are shown. (N = 3, SD error). *p
    Figure Legend Snippet: Knockdown of THBS1 sensitizes cells to CB-5083 and promotes apoptosis. ( A ) HCT116 cells transfected with siControl or siTHBS1-1 (shortly siTHBS1) were treated with or without 0.75 μM CB-5083 for 8 h. Total extracts were analyzed by immunoblotting. Actin was used as a loading control. ( B ) HCT116 cells transfected with siControl or siTHBS1 were treated with or without 0.375 μM CB-5083 for 72 h. Cells stained with crystal violet and are shown. (N = 3, SD error). *p

    Techniques Used: Transfection, Staining

    Related Articles

    Clone Assay:

    Article Title: Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells
    Article Snippet: Luciferase signal was measured in a plate reader. .. Human THBS1 cloned into pCMV3-Flag-THBS1 plasmid was purchased from Sino Biological (catalog number HG10508-NF). .. HCT116, HCT115 and RKO cells seeded in 6-well plate in RPMI medium supplemented in 10% FBS were transfected with the THBS1 construct using Lipofectamine method.

    Plasmid Preparation:

    Article Title: Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells
    Article Snippet: Luciferase signal was measured in a plate reader. .. Human THBS1 cloned into pCMV3-Flag-THBS1 plasmid was purchased from Sino Biological (catalog number HG10508-NF). .. HCT116, HCT115 and RKO cells seeded in 6-well plate in RPMI medium supplemented in 10% FBS were transfected with the THBS1 construct using Lipofectamine method.

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  • 86
    Sino Biological pcmv3 flag thbs1 plasmid
    The steady-state level of <t>THBS1</t> protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).
    Pcmv3 Flag Thbs1 Plasmid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv3 flag thbs1 plasmid/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcmv3 flag thbs1 plasmid - by Bioz Stars, 2021-07
    86/100 stars
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    The steady-state level of THBS1 protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).

    Journal: Cell Cycle

    Article Title: Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells

    doi: 10.1080/15384101.2020.1754584

    Figure Lengend Snippet: The steady-state level of THBS1 protein is increased by CB-5083. ( A ) HCT116 cells were treated with the indicated concentrations of CB-5083, Bortezomib and MLN4924 for 8 h. The steady-state levels of THBS1 and indicated proteins were analyzed by immunoblotting. Actin was used as a loading control. ( B ) Relative abundance of THBS1 protein was quantified (N = 3, SD error). ( C ) Relative abundance of known biomarkers of p97 inhibition (HERP1, CHOP and ATF4) was quantified (N = 3, SD error). ( D ) Mean fold changes (N = 3, SD error) for CDT1 and p21 with 3 μM CB-5083, 1 μM Bortezomib and 3 μM MLN4924. ( E ) An immunofluorescence assay for THBS1 (green), Actin (red) and DAPI (blue) in HCT116 cells treated with or without 1.5 μM CB-5083 for 8 h (N = 3, SD error).

    Article Snippet: Human THBS1 cloned into pCMV3-Flag-THBS1 plasmid was purchased from Sino Biological (catalog number HG10508-NF).

    Techniques: Inhibition, Immunofluorescence

    Knockdown of THBS1 sensitizes cells to CB-5083 and promotes apoptosis. ( A ) HCT116 cells transfected with siControl or siTHBS1-1 (shortly siTHBS1) were treated with or without 0.75 μM CB-5083 for 8 h. Total extracts were analyzed by immunoblotting. Actin was used as a loading control. ( B ) HCT116 cells transfected with siControl or siTHBS1 were treated with or without 0.375 μM CB-5083 for 72 h. Cells stained with crystal violet and are shown. (N = 3, SD error). *p

    Journal: Cell Cycle

    Article Title: Thrombospondin-1 counteracts the p97 inhibitor CB-5083 in colon carcinoma cells

    doi: 10.1080/15384101.2020.1754584

    Figure Lengend Snippet: Knockdown of THBS1 sensitizes cells to CB-5083 and promotes apoptosis. ( A ) HCT116 cells transfected with siControl or siTHBS1-1 (shortly siTHBS1) were treated with or without 0.75 μM CB-5083 for 8 h. Total extracts were analyzed by immunoblotting. Actin was used as a loading control. ( B ) HCT116 cells transfected with siControl or siTHBS1 were treated with or without 0.375 μM CB-5083 for 72 h. Cells stained with crystal violet and are shown. (N = 3, SD error). *p

    Article Snippet: Human THBS1 cloned into pCMV3-Flag-THBS1 plasmid was purchased from Sino Biological (catalog number HG10508-NF).

    Techniques: Transfection, Staining