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PlasmidFactory pcmv lacz plasmid
Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with <t>pCMV</t> backbone, pCMV <t>lacZ,</t> enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P
Pcmv Lacz Plasmid, supplied by PlasmidFactory, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcmv lacz plasmid/product/PlasmidFactory
Average 88 stars, based on 9 article reviews
Price from $9.99 to $1999.99
pcmv lacz plasmid - by Bioz Stars, 2020-09
88/100 stars

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1) Product Images from "Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment"

Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

Journal: Molecular Therapy. Methods & Clinical Development

doi: 10.1038/mtm.2014.12

Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P
Figure Legend Snippet: Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P

Techniques Used: Plasmid Preparation, Expressing, Mouse Assay

β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P
Figure Legend Snippet: β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Electroporation, Plasmid Preparation

β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.
Figure Legend Snippet: β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.

Techniques Used: Expressing, Staining, Plasmid Preparation, Quantitative RT-PCR, Electroporation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

Related Articles

Transfection:

Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment
Article Snippet: .. Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter. .. The EEV plasmid was created by incorporating a SFV DNA replicase sequence (kindly donated by Prof Greg Atkins, Virus Group, Department of Microbiology, School of Genetics and Microbiology, Trinity College Dublin).

Plasmid Preparation:

Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment
Article Snippet: .. Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter. .. The EEV plasmid was created by incorporating a SFV DNA replicase sequence (kindly donated by Prof Greg Atkins, Virus Group, Department of Microbiology, School of Genetics and Microbiology, Trinity College Dublin).

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    PlasmidFactory pcmv lacz plasmid
    Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with <t>pCMV</t> backbone, pCMV <t>lacZ,</t> enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P
    Pcmv Lacz Plasmid, supplied by PlasmidFactory, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv lacz plasmid/product/PlasmidFactory
    Average 88 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    pcmv lacz plasmid - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: Antitumor effects of nontherapeutic plasmid. ( a ) Tumor growth curve of CT26 tumors treated with pCMV backbone, pCMV lacZ, enhanced expression vector (pEEV) backbone, pEEV lacZ, and an untreated tumor growing on Balb/c mice ( n = 6 per group) is presented as tumor diameter measured. Tumor volume was calculated as previously described. Data presented are means ± SEM of six individual tumors. *Compared to the untreated group and pEEV lacZ, * P

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Plasmid Preparation, Expressing, Mouse Assay

    β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: β-galactosidase expression in murine tissue. ( a ) Copy numbers of lacZ transgene ascertained by quantitative polymerase chain reaction (PCR) in murine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNA 2 days after electroporation obtained from MF1-nu/nu mouse tissue. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each quantiative PCR (qPCR). qPCR values are means ± standard error of the mean (SEM) of triplicate measurements. A comparison of enhanced expression vector (pEEV) (light gray bars) and pCMV (dark gray bars) samples were performed. *** P

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Electroporation, Plasmid Preparation

    β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Development and characterization of an enhanced nonviral expression vector for electroporation cancer treatment

    doi: 10.1038/mtm.2014.12

    Figure Lengend Snippet: β-Galactosidase expression in porcine tissue. ( a ) Representative image of β-galactosidase staining in porcine tissues. β-Galactosidase expression significantly increased in enhanced expression vector (pEEV) lacZ in all tissues been examined. (b) Evaluation of lacZ mRNA transcript expression by qRT-PCR in porcine tissue. Bar graph presenting the relative expression of the lacZ mRNA 2 days after electroporation. All qPCR data were normalized using 18S RNA as reference gene. Relative expression levels are plotted as means ± standard error of the mean (SEM) of triplicate measurements. pEEV (light gray bars) and pCMV (dark gray bars). pEEV lacZ expressed was significantly higher than pCMV lacZ. ( c ) Copy numbers of lacZ transgene ascertained by quantitative PCR in porcine tissue. Bar graph showing absolute copy number of the lacz transgene per nanogram of genomic DNAs 2 days after electroporation. All gDNA samples were normalized to 100 ng of DNA prior to PCR. Each individual sample was analyzed in triplicate for each qPCR. qPCR values are means ± SEM of triplicate measurements. A comparison of pEEV (light gray bars) and pCMV (dark gray bars) samples were performed.

    Article Snippet: Plasmids For plasmid transfection, a pCMV-lacz plasmid (Plasmid factory) was used encoding a β-galactosidase protein (LacZ) gene under the control of a CMV promoter.

    Techniques: Expressing, Staining, Plasmid Preparation, Quantitative RT-PCR, Electroporation, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction

    Growth curve for E. coli DH5α-pCMV-lacZ . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: ColE1-Plasmid Production in Escherichia coli: Mathematical Simulation and Experimental Validation

    doi: 10.3389/fbioe.2015.00127

    Figure Lengend Snippet: Growth curve for E. coli DH5α-pCMV-lacZ . The growth curve based on OD 600 measurements is marked by small black circles and the appropriate best-fit curve is indicated in blue. The harvesting time points T 1 , T 2 , and T 3 are presented as bold big points. The minimum–maximum area, within which the measurement values have to reside, is bordered by the red curve (minimal measured OD 600 value) and the green curve (maximal measured OD 600 value).

    Article Snippet: Bacterial strain and plasmids The Escherichia coli strain DH5α (F–Φ80lac ZΔM15 Δ(lac ZYA-arg F) U169 rec A1 end A1 hsd R17 (rK−, mK+) pho A sup E44 λ− thi -1 gyr A96 rel A1) (Source: Plasmid Factory, Bielefeld, Germany) was used as a host strain for transformation of the high copy plasmid pCMV-lacZ (Source: Plasmid Factory, Bielefeld, Germany) as well as the low copy plasmid pSUP 201-3 (Simon et al., ). pCMV-lacZ is a ColE1-derived high copy plasmid for therapeutic pDNA production with a size of 7164 bp.

    Techniques: