Journal: BioMedical Engineering OnLine

Article Title: Advances in 3D-printed scaffold technologies for bone defect repair: materials, biomechanics, and clinical prospects

doi: 10.1186/s12938-025-01381-w

Figure Lengend Snippet: Features of growth factors related to bone tissue engineering and materials

Article Snippet: They incorporated the metal nanomineral zinc oxide (n-BD) into the PCL polymer to enhance biological activity and osteogenic capacity, resulting in an n-BPC bioactive scaffold with interconnected large pores.

Techniques: Animal Model, Cell Differentiation, Activity Assay, Permeability

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Parameters of the centrifugal spinning (CF) using the Nanocent demonstrator.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques:

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: SEM images of the 3D fiber constructs prepared from PLA/PCL blend. Weight ratio: 5/1; disk rotation speed: 1500 rpm. Scale bars: 100 µm ( A ), 30 µm ( B ), and 10 µm ( C ). SEM microscope: Phenom G2.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Construct, Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: SEM image of the 3D fiber constructs prepared from PLA/PCL blend. Weight ratio: 13.5/4; disk rotation speed: 1500 rpm ( A , B ), 3000 rpm ( C , D ), or 5000 rpm ( E , F ). Scale bars: 100 µm ( A , C , E ) and B 50 µm ( B , D , F ). SEM microscope: FIB-SEM, LYRA3 GMU, Tescan.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Construct, Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: SEM image of a fiber of the 3D construct prepared from PLA/PCL blend. Weight ratio: 13.5/4—A fiber detail. SEM microscope: FIB-SEM, LYRA3 GMU, Tescan.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Construct, Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Results of BET analysis of the 3D fibrous scaffolds.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Pore Size

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Results of BET analysis of the micro/nanofibrous scaffolds prepared from the 5/1 and 13.5/4 ( w / w ) PLA/PCL blends: pore size distribution histograms.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Pore Size

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Results of BET analysis of the micro/nanofibrous scaffolds prepared from the 5/1 and 13.5/4 ( w / w ) PLA/PCL blends: nitrogen adsorption and desorption isotherms.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Adsorption

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: The thickness of the layer into which cells migrated on different types of scaffolds. Mean ± S.D. of two–three samples for each experimental group (ANOVA, Student–Newman–Keuls method).

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques:

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: ASC colonization of nanofibrous scaffolds composed of PLA, PLA/PHB 4/1 w / w , and PLA/PCL 13.5/4 ( w / w ) on day 10 after seeding. Fluorescence staining of F-actin in cells with phalloidin (red), and cell nuclei counterstained with DAPI (blue). Images were taken either from the top of the scaffolds or as a 3D side view. Andor Dragonfly 503 confocal microscope, obj. 20×, scale bars 150 µm.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Fluorescence, Staining, Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: The penetration depth of ASCs in PLA/PCL 13.5/4 ( w / w ) micro/nanofibrous scaffolds on days 2, 6, and 10 after cell seeding. Fluorescence staining of F-actin in cells with phalloidin (red), and nuclei counterstained with DAPI (blue). Images were taken as 3D side views. Andor Dragonfly 503 confocal microscope, obj. 20×, scale bar 150 µm.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Fluorescence, Staining, Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: The population density of ASCs on the surface PLA/PCL 13.5/4 ( w / w ) scaffolds on days 2, 6, and 10 after seeding. Mean ± S.D. from four images. ANOVA, Student–Newman–Keuls method. No significant differences in cell number were detected.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques:

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: The time course of ASC penetration into PLA/PCL 13.5/4 ( w / w ) and PLA/PCL 5/1 ( w / w ) scaffolds on days 2, 6, and 10 after seeding. Mean ± S.D. from two–three images for each experimental group and time interval. ANOVA, Student–Newman–Keuls method. *: p ≤ 0.05 compared to day 2; #: p ≤ 0.05 compared to PLA/PCL 5/1 ( w / w ).

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques:

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Morphology of ASCs on the PLA/PCL 13.5/4 ( w / w ) micro/nanofibrous scaffolds on day 6 after cell seeding. Actin filaments–phalloidin (red); cell nuclei–DAPI (blue). Andor Dragonfly 503 confocal microscope, obj. 20×, zoom 2×, scale bars 50 µm.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Microscopy

Journal: Polymers

Article Title: Evaluation of Polymeric Micro/Nanofibrous Hybrid Scaffolds Prepared via Centrifugal Nozzleless Spinning for Tissue Engineering Applications

doi: 10.3390/polym17030386

Figure Lengend Snippet: Colonization of the PLA/PCL 13.5/4 ( w / w ) micro/nanofibrous scaffolds with ASCs on days 2, 6, and 10 of cultivation in an adipogenic medium. Fluorescence staining of F-actin in cells with phalloidin (red), and nuclei counterstained with DAPI (blue). Images were taken either from the top of the scaffolds or as a 3D side view. Andor Dragonfly 503 confocal microscope, obj. 20×, scale bars 100 µm.

Article Snippet: Fourier-transform infrared (FTIR) spectrometer Nicolet iS5 (Fisher Scientific, Waltham, MA, USA) with a diamond crystal iD7 ATR accessory; Figure S9: Designation of functional groups of the PLA and PCL polymers on FTIR spectrum a blended PLA and PCL material (help to identify the functional groups in the previous figure); Figure S10: Scheme of the demonstrator NANOCENT for nozzleless centrifugal spinning; Figure S11: Histograms of PLA/PCL 13,5/4 fiber diameters obtained by the centrifugal spinning at different rotation speed.

Techniques: Fluorescence, Staining, Microscopy