Structured Review

Thermo Fisher pcdna i
Pcdna I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna i/product/Thermo Fisher
Average 88 stars, based on 5 article reviews
Price from $9.99 to $1999.99
pcdna i - by Bioz Stars, 2020-09
88/100 stars

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Clone Assay:

Article Title: Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein
Article Snippet: .. Molecular clones of FB29, TR1.3, or W102G in pUC19B ( ) were first digested with Asc I and Bsa AI enzymes to prepare MLV env constructs. env gene fragments were agarose gel purified, and overhanging ends were filled in with the Klenow fragment of Escherichia coli DNA polymerase I (Promega) and then ligated into pcDNA I (Invitrogen) at the Eco RV site. ..

Agarose Gel Electrophoresis:

Article Title: Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein
Article Snippet: .. Molecular clones of FB29, TR1.3, or W102G in pUC19B ( ) were first digested with Asc I and Bsa AI enzymes to prepare MLV env constructs. env gene fragments were agarose gel purified, and overhanging ends were filled in with the Klenow fragment of Escherichia coli DNA polymerase I (Promega) and then ligated into pcDNA I (Invitrogen) at the Eco RV site. ..

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

Synthesized:

Article Title: Flk-1, a Receptor for Vascular Endothelial Growth Factor (VEGF), Is Expressed by Retinal Progenitor Cells
Article Snippet: .. Digoxigenin-labeled riboprobes were synthesized by using linearized DNA templates in pcDNA I or pcDNA II vectors (Invitrogen). .. Digoxigenin-labeled riboprobes were synthesized by using linearized DNA templates in pcDNA I or pcDNA II vectors (Invitrogen).

Construct:

Article Title: Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein
Article Snippet: .. Molecular clones of FB29, TR1.3, or W102G in pUC19B ( ) were first digested with Asc I and Bsa AI enzymes to prepare MLV env constructs. env gene fragments were agarose gel purified, and overhanging ends were filled in with the Klenow fragment of Escherichia coli DNA polymerase I (Promega) and then ligated into pcDNA I (Invitrogen) at the Eco RV site. ..

Purification:

Article Title: Induction of Syncytia by Neuropathogenic Murine Leukemia Viruses Depends on Receptor Density, Host Cell Determinants, and the Intrinsic Fusion Potential of Envelope Protein
Article Snippet: .. Molecular clones of FB29, TR1.3, or W102G in pUC19B ( ) were first digested with Asc I and Bsa AI enzymes to prepare MLV env constructs. env gene fragments were agarose gel purified, and overhanging ends were filled in with the Klenow fragment of Escherichia coli DNA polymerase I (Promega) and then ligated into pcDNA I (Invitrogen) at the Eco RV site. ..

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

Incubation:

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

Expressing:

Article Title: Functional Analysis of Tpr: Identification of Nuclear Pore Complex Association and Nuclear Localization Domains and a Role in mRNA Export
Article Snippet: .. Plasmid Constructions The expression vector pHA1 was a gift from Dr. Michael Green (University of Massachusetts, Worcester, MA) and contains a cDNA encoding the 13–amino acid influenza virus HA epitope ( ) just upstream of the HindIII site in the polylinker of pcDNA I (Invitrogen, Madison, WI). .. HA-tagged Tpr constructs were obtained by insertion of the appropriate Tpr cDNA fragments into the polylinker of pHA1. cDNA fragments encoding the desired Tpr sequences were obtained from either pT7Tpr, which contains the full-length human Tpr cDNA (a gift from Dr. Larry Gerace, Scripps Institute, La Jolla, CA; ) or from Hela cDNA clones λZ203-1 and λZ203-5, which together encode all but the first 67 amino acids of Tpr ( ).

Article Title: The Drosophila Homolog of Mammalian Zinc Finger Factor MTF-1 Activates Transcription in Response to Heavy Metals
Article Snippet: .. After reconstruction of the full-length dMTF-1 open reading frame in pBluescript SK(−), the entire cDNA was subcloned into pcDNA I (Invitrogen) containing the CMV promoter or pAc5* and pT vectors and was used as the expression vectors for dMTF-1 in mammalian or Drosophila cells, respectively. ..

Polymerase Chain Reaction:

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

Gel Extraction:

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

Plasmid Preparation:

Article Title: Functional Analysis of Tpr: Identification of Nuclear Pore Complex Association and Nuclear Localization Domains and a Role in mRNA Export
Article Snippet: .. Plasmid Constructions The expression vector pHA1 was a gift from Dr. Michael Green (University of Massachusetts, Worcester, MA) and contains a cDNA encoding the 13–amino acid influenza virus HA epitope ( ) just upstream of the HindIII site in the polylinker of pcDNA I (Invitrogen, Madison, WI). .. HA-tagged Tpr constructs were obtained by insertion of the appropriate Tpr cDNA fragments into the polylinker of pHA1. cDNA fragments encoding the desired Tpr sequences were obtained from either pT7Tpr, which contains the full-length human Tpr cDNA (a gift from Dr. Larry Gerace, Scripps Institute, La Jolla, CA; ) or from Hela cDNA clones λZ203-1 and λZ203-5, which together encode all but the first 67 amino acids of Tpr ( ).

Article Title: Immune Responses Induced by Gene Gun or Intramuscular Injection of DNA Vaccines That Express Immunogenic Regions of the Serine Repeat Antigen from Plasmodium falciparum
Article Snippet: .. The SE47′ gene was excised from the pcDNA I:Neo vector by using Hin dIII (partial digest) and Bam HI and inserted into the pcdna3 vector (InVitrogen). .. This new plasmid construct was named pcdna3 SERA 17-382.

Article Title: The Ability of CD40L, but Not Lipopolysaccharide, To Initiate Immunoglobulin Switching to Immunoglobulin G1 Is Explained by Differential Induction of NF-?B/Rel Proteins
Article Snippet: .. The Hin dIII fragment from pcDNA-I containing the p50 coding region ( ) was inserted into the Hin dIII site of the pcDNA-3 vector (Invitrogen, San Diego, Calif.). .. Then, p50 cDNA was excised from pcDNA-3 with Eco RV and Xba I digestion and cloned into pEF1α-neo vector digested with Eco RI and Xba I.

Article Title: Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status
Article Snippet: .. The PCR product was purified by agarose gel electrophoresis and QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany), incubated with Taq DNA polymerase (Invitrogen), cloned into pCR® 2.1-TOPO Vector (Invitrogen), released by BamHI and XhoI digestion and subcloned into pcDNA I (Invitrogen). .. 20 μg of plasmid DNA was mixed with 120 μl Lipofectamine (Invitrogen) in 2.8 ml Opti-MEM (Invitrogen) and incubated for 20–30 min at room temperature.

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  • 95
    Thermo Fisher t rex complete kit
    T Rex Complete Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t rex complete kit/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
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    93
    Thermo Fisher i recombinant pcdna3 1 padi3 rfp plasmids
    RNA-sequencing analysis of <t>PADI3-expressing</t> HCT116 cells. (A) Heat map from the RNA-sequencing analysis. (B) Expression of phosphorylated AKT (p-AKT), AKT, Sirt2, Snail and p21 in OE-PADI3 HCT116 cells, <t>OE-RFP</t> HCT116 cells is selected as the control group. (C) Western blot analysis was used to measure the expression level of Sirt2 and PADI3 in colon cancer tissues and corresponding tissues. (D) Statistical analysis of the expression level of Sirt2 and PADI3. * indicates P
    I Recombinant Pcdna3 1 Padi3 Rfp Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/i recombinant pcdna3 1 padi3 rfp plasmids/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
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    92
    Thermo Fisher pcdna3 0 i κbαm
    RNA-sequencing analysis of <t>PADI3-expressing</t> HCT116 cells. (A) Heat map from the RNA-sequencing analysis. (B) Expression of phosphorylated AKT (p-AKT), AKT, Sirt2, Snail and p21 in OE-PADI3 HCT116 cells, <t>OE-RFP</t> HCT116 cells is selected as the control group. (C) Western blot analysis was used to measure the expression level of Sirt2 and PADI3 in colon cancer tissues and corresponding tissues. (D) Statistical analysis of the expression level of Sirt2 and PADI3. * indicates P
    Pcdna3 0 I κbαm, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher transfection i e pcdna3 only
    RNA-sequencing analysis of <t>PADI3-expressing</t> HCT116 cells. (A) Heat map from the RNA-sequencing analysis. (B) Expression of phosphorylated AKT (p-AKT), AKT, Sirt2, Snail and p21 in OE-PADI3 HCT116 cells, <t>OE-RFP</t> HCT116 cells is selected as the control group. (C) Western blot analysis was used to measure the expression level of Sirt2 and PADI3 in colon cancer tissues and corresponding tissues. (D) Statistical analysis of the expression level of Sirt2 and PADI3. * indicates P
    Transfection I E Pcdna3 Only, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    RNA-sequencing analysis of PADI3-expressing HCT116 cells. (A) Heat map from the RNA-sequencing analysis. (B) Expression of phosphorylated AKT (p-AKT), AKT, Sirt2, Snail and p21 in OE-PADI3 HCT116 cells, OE-RFP HCT116 cells is selected as the control group. (C) Western blot analysis was used to measure the expression level of Sirt2 and PADI3 in colon cancer tissues and corresponding tissues. (D) Statistical analysis of the expression level of Sirt2 and PADI3. * indicates P

    Journal: Cancer Biology & Medicine

    Article Title: PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

    doi: 10.20892/j.issn.2095-3941.2019.0065

    Figure Lengend Snippet: RNA-sequencing analysis of PADI3-expressing HCT116 cells. (A) Heat map from the RNA-sequencing analysis. (B) Expression of phosphorylated AKT (p-AKT), AKT, Sirt2, Snail and p21 in OE-PADI3 HCT116 cells, OE-RFP HCT116 cells is selected as the control group. (C) Western blot analysis was used to measure the expression level of Sirt2 and PADI3 in colon cancer tissues and corresponding tissues. (D) Statistical analysis of the expression level of Sirt2 and PADI3. * indicates P

    Article Snippet: RFP was selected as the reporter gene, which was inserted into the multiple cloning site (MCS) using the restriction enzymes Kpn I and Not I. Recombinant pCDNA3.1-PADI3-RFP plasmids were purified using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, USA) and stored at –80°C.

    Techniques: RNA Sequencing Assay, Expressing, Western Blot

    Effects of PADI3 on HCT116 and LoVo cells. RFP-expressing cells were used as controls. (A) Weak PADI3 expression was detected in HCT116 and LoVo cells. (B) Western blot analysis was used to measure PADI3 and RFP expression in both LoVo and HCT116 cells. (C, D) A CCK-8 assay measured the proliferation of HCT116 and LoVo cells. (E) The colony formation ability of HCT116 cells was measured and statistically analyzed using a colony formation assay following 14 days of culture. (F) The colony formation ability of LoVo cells was measured and statistically analyzed using a colony formation assay following 14 days of culture. * indicates P

    Journal: Cancer Biology & Medicine

    Article Title: PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

    doi: 10.20892/j.issn.2095-3941.2019.0065

    Figure Lengend Snippet: Effects of PADI3 on HCT116 and LoVo cells. RFP-expressing cells were used as controls. (A) Weak PADI3 expression was detected in HCT116 and LoVo cells. (B) Western blot analysis was used to measure PADI3 and RFP expression in both LoVo and HCT116 cells. (C, D) A CCK-8 assay measured the proliferation of HCT116 and LoVo cells. (E) The colony formation ability of HCT116 cells was measured and statistically analyzed using a colony formation assay following 14 days of culture. (F) The colony formation ability of LoVo cells was measured and statistically analyzed using a colony formation assay following 14 days of culture. * indicates P

    Article Snippet: RFP was selected as the reporter gene, which was inserted into the multiple cloning site (MCS) using the restriction enzymes Kpn I and Not I. Recombinant pCDNA3.1-PADI3-RFP plasmids were purified using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, USA) and stored at –80°C.

    Techniques: Expressing, Western Blot, CCK-8 Assay, Colony Assay

    Effects of PADI3 in vivo . (A) Western blot analysis was used to measure PADI3 expression. (B) PADI3-expressing HCT116 cells were injected into the left dorsal flank of BALB/c nude mice, and RFP-expressing HCT116 cells were injected into the right dorsal flank. (C) Tumors were dissected 42 days after cell injection. (D) Tumor weight was measured and statistically analyzed. (E) The tumor formation ratio was statistically analyzed. * indicates P

    Journal: Cancer Biology & Medicine

    Article Title: PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

    doi: 10.20892/j.issn.2095-3941.2019.0065

    Figure Lengend Snippet: Effects of PADI3 in vivo . (A) Western blot analysis was used to measure PADI3 expression. (B) PADI3-expressing HCT116 cells were injected into the left dorsal flank of BALB/c nude mice, and RFP-expressing HCT116 cells were injected into the right dorsal flank. (C) Tumors were dissected 42 days after cell injection. (D) Tumor weight was measured and statistically analyzed. (E) The tumor formation ratio was statistically analyzed. * indicates P

    Article Snippet: RFP was selected as the reporter gene, which was inserted into the multiple cloning site (MCS) using the restriction enzymes Kpn I and Not I. Recombinant pCDNA3.1-PADI3-RFP plasmids were purified using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, USA) and stored at –80°C.

    Techniques: In Vivo, Western Blot, Expressing, Injection, Mouse Assay

    Effects of Sirt2 on PADI3-expressing HCT116 cells. PADI3-expressing HCT116 cells were transfected with pCDNA3.1-Sirt2-iso1 plasmids (OE-PADI3-Sirt2-iso1) or pCDNA3.1-Sirt2-iso2 plasmids (OE-PADI3-Sirt2-iso2) separately. PADI3-expressing HCT116 cells were used as the positive control, and RFP-expressing HCT116 cells were used as the negative control. (A) Western blot analysis was used to measure the expression of AKT, p-AKT, Snail and p21. (B) A CCK-8 assay was used to measure cell proliferation. (C) The cell cycle was analyzed using FCM. (D) Statistical analysis of C. (E) Colony number and size were analyzed. (F) Statistical analysis of E. * indicates P

    Journal: Cancer Biology & Medicine

    Article Title: PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

    doi: 10.20892/j.issn.2095-3941.2019.0065

    Figure Lengend Snippet: Effects of Sirt2 on PADI3-expressing HCT116 cells. PADI3-expressing HCT116 cells were transfected with pCDNA3.1-Sirt2-iso1 plasmids (OE-PADI3-Sirt2-iso1) or pCDNA3.1-Sirt2-iso2 plasmids (OE-PADI3-Sirt2-iso2) separately. PADI3-expressing HCT116 cells were used as the positive control, and RFP-expressing HCT116 cells were used as the negative control. (A) Western blot analysis was used to measure the expression of AKT, p-AKT, Snail and p21. (B) A CCK-8 assay was used to measure cell proliferation. (C) The cell cycle was analyzed using FCM. (D) Statistical analysis of C. (E) Colony number and size were analyzed. (F) Statistical analysis of E. * indicates P

    Article Snippet: RFP was selected as the reporter gene, which was inserted into the multiple cloning site (MCS) using the restriction enzymes Kpn I and Not I. Recombinant pCDNA3.1-PADI3-RFP plasmids were purified using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, USA) and stored at –80°C.

    Techniques: Expressing, Transfection, Positive Control, Negative Control, Western Blot, CCK-8 Assay

    Subcellular locations of the truncated forms of PADI3 in HCT116 cells. (A) Red fluorescence indicates RFP or PADI3-fused RFP, and blue indicates nuclei stained with DAPI. The merged image shows both RFP fluorescence and DAPI staining. The scale bar represents 20 μm. (B) Western blot analysis was used to detect truncated PADI3 in the cytoplasm and nucleus. GAPDH was used as the internal control for cytoplasmic expression, and histone 3 was used as the internal control for nuclear expression. Nu: nucleus, Cy: cytoplasm.

    Journal: Cancer Biology & Medicine

    Article Title: PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

    doi: 10.20892/j.issn.2095-3941.2019.0065

    Figure Lengend Snippet: Subcellular locations of the truncated forms of PADI3 in HCT116 cells. (A) Red fluorescence indicates RFP or PADI3-fused RFP, and blue indicates nuclei stained with DAPI. The merged image shows both RFP fluorescence and DAPI staining. The scale bar represents 20 μm. (B) Western blot analysis was used to detect truncated PADI3 in the cytoplasm and nucleus. GAPDH was used as the internal control for cytoplasmic expression, and histone 3 was used as the internal control for nuclear expression. Nu: nucleus, Cy: cytoplasm.

    Article Snippet: RFP was selected as the reporter gene, which was inserted into the multiple cloning site (MCS) using the restriction enzymes Kpn I and Not I. Recombinant pCDNA3.1-PADI3-RFP plasmids were purified using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, USA) and stored at –80°C.

    Techniques: Fluorescence, Staining, Western Blot, Expressing