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Thermo Fisher pcdna gw
Pcdna Gw, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna gw/product/Thermo Fisher
Average 85 stars, based on 3 article reviews
Price from $9.99 to $1999.99
pcdna gw - by Bioz Stars, 2020-09
85/100 stars

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Clone Assay:

Article Title: PGL proteins self associate and bind RNPs to mediate germ granule assembly in C. elegans
Article Snippet: .. For constitutive expression of P-granule components in mammalian cells, the amplified cDNA fragments were cloned into expression vectors containing the cytomegalovirus promoter (pDEST26 for the N-terminal 6xHis tag, pDEST27 for the N-terminal GST tag, or pcDNA-DEST40 for the C-terminal 6xHis tag) by the Gateway system (Invitrogen). pcDNA/GW-40/lacZ for expressing control LacZ::6×His was purchased from Invitrogen. .. For expression of PGL-3, MEX-3, and the PGL-3 variants in the C. elegans germline and early embryos, the coding regions were cloned into the plasmid pID3.01B (from G. Seydoux, Johns Hopkins University School of Medicine, Baltimore, MD) for the GFP tag.

Transfection:

Article Title: EWS/ETS Regulates the Expression of the Dickkopf Family in Ewing Family Tumor Cells
Article Snippet: .. The cells were also transfected with 25 ng of pcDNA/GW-40/LacZ (Invitrogen) for the standardization of transfection efficiency. .. The luciferase activity and β-gal activity were measured 48 h after the transfection as described before (Mon et al. , 2003).

Article Title: Identification of a second gene associated with variation in vertebral number in domestic pigs
Article Snippet: .. NIH-3T3 and HeLa cells (1 × 104 cells/chamber) were seeded on BioCoat Poly-D-Lysine 4-well Culture Slides (BD Biosciences), and then the plasmid vectors pcDNA-DEST47-VRTN and pcDNA/GW-47/CAT (Invitrogen), which encoded a GFP-fused CAT (chloramphenicol acetyltransferase) and was a control for cytoplasmic expression, were transfected into cells by using FuGENE 6 (Roche Diagnostics). .. Forty-eight hours after transfection, the cells were washed and the nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen).

Article Title: Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA
Article Snippet: .. The cells were transfected at 200 ng per well with mRNA expressing either CpsA-GFP or CAT-GFP (control). mRNA was generated from plasmids pJP156 (pDEST47∆ss-CpsA-GFP, described above) and pcDNA/GW-47/CAT (Thermo Fisher Scientific) using the mMESSAGE mMACHINE T7 Ultra kit (Invitrogen) followed by purification using MEGAclear Transcription Cleanup kit (Invitrogen). mRNA was transfected using the Mirus TransIT mRNA kit (Mirus Bio LLC) according to the manufacturer’s instructions. .. The transfected macrophages were challenged with Zymosan A Bioparticle Alexa Fluor 594 conjugate ( ; Molecular Probes, Life Technologies) at 2 µg per well for 6 h. The samples were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Immunostaining was done overnight using the primary antibodies listed above; the secondary antibody (Molecular Probes or Abcam ab175664) was conjugated to Alexa Fluor 405.

Amplification:

Article Title: PGL proteins self associate and bind RNPs to mediate germ granule assembly in C. elegans
Article Snippet: .. For constitutive expression of P-granule components in mammalian cells, the amplified cDNA fragments were cloned into expression vectors containing the cytomegalovirus promoter (pDEST26 for the N-terminal 6xHis tag, pDEST27 for the N-terminal GST tag, or pcDNA-DEST40 for the C-terminal 6xHis tag) by the Gateway system (Invitrogen). pcDNA/GW-40/lacZ for expressing control LacZ::6×His was purchased from Invitrogen. .. For expression of PGL-3, MEX-3, and the PGL-3 variants in the C. elegans germline and early embryos, the coding regions were cloned into the plasmid pID3.01B (from G. Seydoux, Johns Hopkins University School of Medicine, Baltimore, MD) for the GFP tag.

Purification:

Article Title: Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA
Article Snippet: .. The cells were transfected at 200 ng per well with mRNA expressing either CpsA-GFP or CAT-GFP (control). mRNA was generated from plasmids pJP156 (pDEST47∆ss-CpsA-GFP, described above) and pcDNA/GW-47/CAT (Thermo Fisher Scientific) using the mMESSAGE mMACHINE T7 Ultra kit (Invitrogen) followed by purification using MEGAclear Transcription Cleanup kit (Invitrogen). mRNA was transfected using the Mirus TransIT mRNA kit (Mirus Bio LLC) according to the manufacturer’s instructions. .. The transfected macrophages were challenged with Zymosan A Bioparticle Alexa Fluor 594 conjugate ( ; Molecular Probes, Life Technologies) at 2 µg per well for 6 h. The samples were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Immunostaining was done overnight using the primary antibodies listed above; the secondary antibody (Molecular Probes or Abcam ab175664) was conjugated to Alexa Fluor 405.

Generated:

Article Title: Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA
Article Snippet: .. The cells were transfected at 200 ng per well with mRNA expressing either CpsA-GFP or CAT-GFP (control). mRNA was generated from plasmids pJP156 (pDEST47∆ss-CpsA-GFP, described above) and pcDNA/GW-47/CAT (Thermo Fisher Scientific) using the mMESSAGE mMACHINE T7 Ultra kit (Invitrogen) followed by purification using MEGAclear Transcription Cleanup kit (Invitrogen). mRNA was transfected using the Mirus TransIT mRNA kit (Mirus Bio LLC) according to the manufacturer’s instructions. .. The transfected macrophages were challenged with Zymosan A Bioparticle Alexa Fluor 594 conjugate ( ; Molecular Probes, Life Technologies) at 2 µg per well for 6 h. The samples were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Immunostaining was done overnight using the primary antibodies listed above; the secondary antibody (Molecular Probes or Abcam ab175664) was conjugated to Alexa Fluor 405.

Expressing:

Article Title: PGL proteins self associate and bind RNPs to mediate germ granule assembly in C. elegans
Article Snippet: .. For constitutive expression of P-granule components in mammalian cells, the amplified cDNA fragments were cloned into expression vectors containing the cytomegalovirus promoter (pDEST26 for the N-terminal 6xHis tag, pDEST27 for the N-terminal GST tag, or pcDNA-DEST40 for the C-terminal 6xHis tag) by the Gateway system (Invitrogen). pcDNA/GW-40/lacZ for expressing control LacZ::6×His was purchased from Invitrogen. .. For expression of PGL-3, MEX-3, and the PGL-3 variants in the C. elegans germline and early embryos, the coding regions were cloned into the plasmid pID3.01B (from G. Seydoux, Johns Hopkins University School of Medicine, Baltimore, MD) for the GFP tag.

Article Title: Identification of a second gene associated with variation in vertebral number in domestic pigs
Article Snippet: .. NIH-3T3 and HeLa cells (1 × 104 cells/chamber) were seeded on BioCoat Poly-D-Lysine 4-well Culture Slides (BD Biosciences), and then the plasmid vectors pcDNA-DEST47-VRTN and pcDNA/GW-47/CAT (Invitrogen), which encoded a GFP-fused CAT (chloramphenicol acetyltransferase) and was a control for cytoplasmic expression, were transfected into cells by using FuGENE 6 (Roche Diagnostics). .. Forty-eight hours after transfection, the cells were washed and the nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen).

Article Title: Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA
Article Snippet: .. The cells were transfected at 200 ng per well with mRNA expressing either CpsA-GFP or CAT-GFP (control). mRNA was generated from plasmids pJP156 (pDEST47∆ss-CpsA-GFP, described above) and pcDNA/GW-47/CAT (Thermo Fisher Scientific) using the mMESSAGE mMACHINE T7 Ultra kit (Invitrogen) followed by purification using MEGAclear Transcription Cleanup kit (Invitrogen). mRNA was transfected using the Mirus TransIT mRNA kit (Mirus Bio LLC) according to the manufacturer’s instructions. .. The transfected macrophages were challenged with Zymosan A Bioparticle Alexa Fluor 594 conjugate ( ; Molecular Probes, Life Technologies) at 2 µg per well for 6 h. The samples were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Immunostaining was done overnight using the primary antibodies listed above; the secondary antibody (Molecular Probes or Abcam ab175664) was conjugated to Alexa Fluor 405.

Chloramphenicol Acetyltransferase Assay:

Article Title: Identification of a second gene associated with variation in vertebral number in domestic pigs
Article Snippet: .. NIH-3T3 and HeLa cells (1 × 104 cells/chamber) were seeded on BioCoat Poly-D-Lysine 4-well Culture Slides (BD Biosciences), and then the plasmid vectors pcDNA-DEST47-VRTN and pcDNA/GW-47/CAT (Invitrogen), which encoded a GFP-fused CAT (chloramphenicol acetyltransferase) and was a control for cytoplasmic expression, were transfected into cells by using FuGENE 6 (Roche Diagnostics). .. Forty-eight hours after transfection, the cells were washed and the nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen).

Article Title: Mycobacterium tuberculosis is protected from NADPH oxidase and LC3-associated phagocytosis by the LCP protein CpsA
Article Snippet: .. The cells were transfected at 200 ng per well with mRNA expressing either CpsA-GFP or CAT-GFP (control). mRNA was generated from plasmids pJP156 (pDEST47∆ss-CpsA-GFP, described above) and pcDNA/GW-47/CAT (Thermo Fisher Scientific) using the mMESSAGE mMACHINE T7 Ultra kit (Invitrogen) followed by purification using MEGAclear Transcription Cleanup kit (Invitrogen). mRNA was transfected using the Mirus TransIT mRNA kit (Mirus Bio LLC) according to the manufacturer’s instructions. .. The transfected macrophages were challenged with Zymosan A Bioparticle Alexa Fluor 594 conjugate ( ; Molecular Probes, Life Technologies) at 2 µg per well for 6 h. The samples were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Immunostaining was done overnight using the primary antibodies listed above; the secondary antibody (Molecular Probes or Abcam ab175664) was conjugated to Alexa Fluor 405.

Plasmid Preparation:

Article Title: Identification of a second gene associated with variation in vertebral number in domestic pigs
Article Snippet: .. NIH-3T3 and HeLa cells (1 × 104 cells/chamber) were seeded on BioCoat Poly-D-Lysine 4-well Culture Slides (BD Biosciences), and then the plasmid vectors pcDNA-DEST47-VRTN and pcDNA/GW-47/CAT (Invitrogen), which encoded a GFP-fused CAT (chloramphenicol acetyltransferase) and was a control for cytoplasmic expression, were transfected into cells by using FuGENE 6 (Roche Diagnostics). .. Forty-eight hours after transfection, the cells were washed and the nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (Invitrogen).

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  • 99
    Thermo Fisher vector pegfp c1
    Verification of E7 expression in HPV16 E7-transfected NHEKs. NHEKs were transfected with the E7-expression vector pEGFP-16E7 or the control vector <t>pEGFP-C1.</t> E7 was detected by immunostaining (red) and the cell nucleus was stained with DAPI (blue) .
    Vector Pegfp C1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pegfp c1/product/Thermo Fisher
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    vector pegfp c1 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    97
    Thermo Fisher eyfp expressing cells
    Channelrhodopsin-2 expression in inhibitory inferior colliculus central nucleus neurons of mixed CBA/CaJ x C57BL/6J genetic background mice. ( A ) A low magnification image shows that the majority of inhibitory cells labeled with <t>anti-GAD67</t> antibody (red) also express <t>ChR2-EYFP</t> labelled with anti-GFP antibody (green). White arrows show examples of double-labelled cells. The blue arrow shows a rare example of a cell that labeled with the anti-GAD antibody but did not express EYFP. Scale = 20 μm. ( B ) High magnification images of double-labelled cells. Scale = 20 μm.
    Eyfp Expressing Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eyfp expressing cells/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    eyfp expressing cells - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    86
    Thermo Fisher pcdna 6 2 gw emgfp mir expression vector
    Channelrhodopsin-2 expression in inhibitory inferior colliculus central nucleus neurons of mixed CBA/CaJ x C57BL/6J genetic background mice. ( A ) A low magnification image shows that the majority of inhibitory cells labeled with <t>anti-GAD67</t> antibody (red) also express <t>ChR2-EYFP</t> labelled with anti-GFP antibody (green). White arrows show examples of double-labelled cells. The blue arrow shows a rare example of a cell that labeled with the anti-GAD antibody but did not express EYFP. Scale = 20 μm. ( B ) High magnification images of double-labelled cells. Scale = 20 μm.
    Pcdna 6 2 Gw Emgfp Mir Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna 6 2 gw emgfp mir expression vector/product/Thermo Fisher
    Average 86 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    pcdna 6 2 gw emgfp mir expression vector - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Verification of E7 expression in HPV16 E7-transfected NHEKs. NHEKs were transfected with the E7-expression vector pEGFP-16E7 or the control vector pEGFP-C1. E7 was detected by immunostaining (red) and the cell nucleus was stained with DAPI (blue) .

    Journal: Frontiers in Genetics

    Article Title: Digital RNA Sequencing of Human Epidermal Keratinocytes Carrying Human Papillomavirus Type 16 E7

    doi: 10.3389/fgene.2020.00819

    Figure Lengend Snippet: Verification of E7 expression in HPV16 E7-transfected NHEKs. NHEKs were transfected with the E7-expression vector pEGFP-16E7 or the control vector pEGFP-C1. E7 was detected by immunostaining (red) and the cell nucleus was stained with DAPI (blue) .

    Article Snippet: Cell Culture and Cell TransfectionHPV16-containing plasmid DNA (ATCC:45113D) was used as the template for PCR amplification and cloning of the HPV16 E7 gene into vector pEGFP-C1 following protocols described previously.

    Techniques: Expressing, Transfection, Plasmid Preparation, Immunostaining, Staining

    Channelrhodopsin-2 expression in inhibitory inferior colliculus central nucleus neurons of mixed CBA/CaJ x C57BL/6J genetic background mice. ( A ) A low magnification image shows that the majority of inhibitory cells labeled with anti-GAD67 antibody (red) also express ChR2-EYFP labelled with anti-GFP antibody (green). White arrows show examples of double-labelled cells. The blue arrow shows a rare example of a cell that labeled with the anti-GAD antibody but did not express EYFP. Scale = 20 μm. ( B ) High magnification images of double-labelled cells. Scale = 20 μm.

    Journal: bioRxiv

    Article Title: Group II metabotropic glutamate receptors modulate sound evoked and spontaneous activity in the mouse inferior colliculus

    doi: 10.1101/2020.06.03.130724

    Figure Lengend Snippet: Channelrhodopsin-2 expression in inhibitory inferior colliculus central nucleus neurons of mixed CBA/CaJ x C57BL/6J genetic background mice. ( A ) A low magnification image shows that the majority of inhibitory cells labeled with anti-GAD67 antibody (red) also express ChR2-EYFP labelled with anti-GFP antibody (green). White arrows show examples of double-labelled cells. The blue arrow shows a rare example of a cell that labeled with the anti-GAD antibody but did not express EYFP. Scale = 20 μm. ( B ) High magnification images of double-labelled cells. Scale = 20 μm.

    Article Snippet: For one series, free-floating sections were stained for GAD67 to confirm that EYFP-expressing cells in the IC were indeed GABAergic.

    Techniques: Expressing, Crocin Bleaching Assay, Mouse Assay, Labeling