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GenePharma Company pcdna aeg1
The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
Pcdna Aeg1, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna aeg1/product/GenePharma Company
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pcdna aeg1 - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma"

Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

doi: 10.12659/MSM.906616

The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
Figure Legend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

Techniques Used: Expressing, Cotransfection

The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p
Figure Legend Snippet: The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

Techniques Used: Expressing, Transfection, Cotransfection

miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p
Figure Legend Snippet: miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

Techniques Used: Cotransfection, Activity Assay, Transfection, Migration

2) Product Images from "Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma"

Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

doi: 10.12659/MSM.906616

The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p
Figure Legend Snippet: The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

Techniques Used: Expressing, Transfection, Cotransfection

The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
Figure Legend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

Techniques Used: Expressing, Cotransfection

miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p
Figure Legend Snippet: miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

Techniques Used: Cotransfection, Activity Assay, Transfection, Migration

Related Articles

Transfection:

Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma
Article Snippet: .. Plasmid construction and transfection miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China). ..

Plasmid Preparation:

Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma
Article Snippet: .. Plasmid construction and transfection miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China). ..

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    GenePharma Company pcdna aeg1
    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the <t>AEG1</t> level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of <t>pcDNA-TUG1</t> in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p
    Pcdna Aeg1, supplied by GenePharma Company, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna aeg1/product/GenePharma Company
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pcdna aeg1 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Cotransfection

    The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Transfection, Cotransfection

    miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Article Snippet: miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Cotransfection, Activity Assay, Transfection, Migration

    The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The interaction among TUG1, miR-129-5p, and AEG1 in A375 cells. ( A, B ) Silencing of TUG1 promoted the expression of miR-129-5p, but conversely, upregulation of TUG1 depressed the expression of miR-129-5p. ( C ) The transfection of miR-129-5p mimics suppressed the expression of AEG1 in A375 cells, but the cotransfection of miR-129-5p mimics and pcDNA-TUG1 reversed the inhibiting function of miR-129-5p on the expression of AEG1 in A375 cells. ( D ) High expression of TUG1 enhanced AEG1 expression, but the cotransfection of miR-129-5p mimics with pcDNA-TUG1 alleviated the promoting function of pcDNA-TUG1 on the expression of AEG1 in A375 cells. ( E ) Silencing of TUG1 distinctly inhibited the expression of AEG1, but miR-129-5p inhibitors attenuated the inhibitory function of sh-TUG1 on the expression of AEG1 in A375 cells. * p

    Article Snippet: Plasmid construction and transfection miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Transfection, Cotransfection

    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Article Snippet: Plasmid construction and transfection miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Expressing, Cotransfection

    miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: miR-129-5p and AEG1 mediated the function of TUG1 on the growth and metastasis of A375 cells. ( A ) The cotransfection of sh-TUG1 with pcDNA-AEG1 alleviated the inhibitory ability of sh-TUG1 on the proliferation and metastasis of A375 cells in comparison with sh-TUG1 group. ( B ) The cotransfection of miR-129-5p inhibitor enhanced the proliferation and metastasis activity of A375 cells transfected with sh-TUG1 in comparison with sh-TUG1 group. ( C, D ) miR-129-5p mimics or sh-AEG1 abrogated the growth, migration, and invasion of A375 cells transfected with pcDNA-TUG1. * p

    Article Snippet: Plasmid construction and transfection miR-129-5p mimics, miR-129-5p inhibitors, sh-TUG1, pcDNA-TUG1, sh-AEG1, and pcDNA-AEG1 were purchased from GenePharma (Shanghai, China).

    Techniques: Cotransfection, Activity Assay, Transfection, Migration