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Cambridge Isotope Laboratories pcbs
GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , <t>PBDEs</t> (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and <t>PCBs</t> (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .
Pcbs, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)"

Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)

Journal: Journal of Analytical Methods in Chemistry

doi: 10.1155/2017/9294024

GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .
Figure Legend Snippet: GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .

Techniques Used: Gas Chromatography-Mass Spectrometry, Mass Spectrometry, Fluorescence In Situ Hybridization

The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.
Figure Legend Snippet: The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.

Techniques Used:

Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.
Figure Legend Snippet: Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.

Techniques Used: Standard Deviation, Fluorescence In Situ Hybridization, Chromatography

2) Product Images from "Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)"

Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)

Journal: Journal of Analytical Methods in Chemistry

doi: 10.1155/2017/9294024

GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .
Figure Legend Snippet: GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .

Techniques Used: Gas Chromatography-Mass Spectrometry, Mass Spectrometry, Fluorescence In Situ Hybridization

The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.
Figure Legend Snippet: The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.

Techniques Used:

Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.
Figure Legend Snippet: Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.

Techniques Used: Standard Deviation, Fluorescence In Situ Hybridization, Chromatography

Related Articles

other:

Article Title: Selective and comprehensive analysis of organohalogen compounds by GC × GC–HRTofMS and MS/MS
Article Snippet: Chemicals Carbon-13-labeled and carbon-13-unlabeled polychlorinated dibenzo-p -dioxins (PCDDs) were obtained from Wellington Laboratories Inc. (Guelph, ON, Canada); PCDFs, PCBs, and PBDEs from Cambridge Isotope Laboratories Inc. (Tewkesbury, MA, USA); other 13 C-labeled and 13 C-unlabeled POPs from Wako Pure Chemical Industries, Ltd. (Osaka, Japan); and 263 unlabeled pesticides from Kanto Chemical Co., Inc. (Tokyo, Japan).

Article Title: Analysis method for PCBs in reclaimed oil using a fast-GC triple stage quadrupole mass spectrometer with the 13-component quantitation method
Article Snippet: Regents, chemicals, and sample Calibration solution of PCBs: EC-5488 and internal standard solutions; EC-5379, and EC-5450 were purchased from Cambridge Isotope Laboratories Inc. (MA, USA).

Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)
Article Snippet: Considering the cost factor and similar property between PBDEs and PCBs, the PBDEs and PCBs had the same SS and IS, which were 2,2′,3,4,5,5′-hexaCB (13 C12 , 99%, EC-1418-1.2, Cambridge Isotope Laboratories, Inc., USA) (141) and 2,2′,3,3′,4,5,5′,6,6′-nonaCB (13 C12 , 99%, EC-1419-1.2, Cambridge Isotope Laboratories, Inc., USA) (208), respectively.

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    Cambridge Isotope Laboratories pcbs
    GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , <t>PBDEs</t> (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and <t>PCBs</t> (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .
    Pcbs, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcbs/product/Cambridge Isotope Laboratories
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcbs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    93
    Cambridge Isotope Laboratories dl pcb analysis
    The role of hydrological factors (high, stable and low water flow) on total <t>PCDD</t> + PCDF + <t>dl-PCB</t> and total TEQ loads transported along the continuum of the Pilica River at Sulejów, Tomaszów Maz. and Spała river gauge stations.
    Dl Pcb Analysis, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dl pcb analysis/product/Cambridge Isotope Laboratories
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dl pcb analysis - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    89
    Cambridge Isotope Laboratories pcb 126
    PCB 126-induced oxidative stress is modulated by green tea extract (GTE) diet supplementation. Plasma F 2 -isoprostane (A) and metabolite (B) levels were measured by HPLC/MS MS to assess in vivo oxidative stress induced by <t>PCB</t> 126 that is potentially mitigated by GTE supplementation. Relative levels of combined F-2 IsoPs, including PGF2α, 8-iso-PGF2α, iPF2α-III, 8-epiPGF2α, 8-isoprostane, and 15-F2t isoprostanes, were determined by averaging the AUC integration values from retention times of 8 and 11.3 minutes (Q1 = 353.144, Q2 =193.1). Additionally, the level of 13,14-dihydro-15-ketoPGF2α, an F-2 IsoP metabolite, was determined by integrating its peak at 11.3 minutes (Q1 = 355.2, Q2 = 311.4). Data are presented as mean±S.E.M. (n=8–10). GTE supplementation significantly decreased oxidative stress induced by PCB 126 treatment (*p
    Pcb 126, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcb 126/product/Cambridge Isotope Laboratories
    Average 89 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)

    doi: 10.1155/2017/9294024

    Figure Lengend Snippet: GC/MS or GC/MS/MS chromatogram for the analysis of fish meat tissue spiked with PAHs (chart a) at 100 ng g −1 , OCPs (chart b) at 50 ng g −1 , PBDEs (chart c) at 25 ng g −1 (PBDE 209 at 125 ng g −1 ), and PCBs (chart d) at 25 ng g −1 using scheme #1 in Figure 4 .

    Article Snippet: Considering the cost factor and similar property between PBDEs and PCBs, the PBDEs and PCBs had the same SS and IS, which were 2,2′,3,4,5,5′-hexaCB (13 C12 , 99%, EC-1418-1.2, Cambridge Isotope Laboratories, Inc., USA) (141) and 2,2′,3,3′,4,5,5′,6,6′-nonaCB (13 C12 , 99%, EC-1419-1.2, Cambridge Isotope Laboratories, Inc., USA) (208), respectively.

    Techniques: Gas Chromatography-Mass Spectrometry, Mass Spectrometry, Fluorescence In Situ Hybridization

    The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)

    doi: 10.1155/2017/9294024

    Figure Lengend Snippet: The fraction of qualified target chemicals (cumulative recovery value > 70%) with increase of elute volume. The charts (a, b, c, d, and e) denote PAHs, OCPs, PBDEs, PCBs, and average fraction of those four groups of target chemicals.

    Article Snippet: Considering the cost factor and similar property between PBDEs and PCBs, the PBDEs and PCBs had the same SS and IS, which were 2,2′,3,4,5,5′-hexaCB (13 C12 , 99%, EC-1418-1.2, Cambridge Isotope Laboratories, Inc., USA) (141) and 2,2′,3,3′,4,5,5′,6,6′-nonaCB (13 C12 , 99%, EC-1419-1.2, Cambridge Isotope Laboratories, Inc., USA) (208), respectively.

    Techniques:

    Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Optimized Multiresidue Analysis of Organic Contaminants of Priority Concern in a Daily Consumed Fish (Grass Carp)

    doi: 10.1155/2017/9294024

    Figure Lengend Snippet: Recoveries (RV, the column), their standard deviation (SD, the error bar), and their relative standard deviation (RSD, the line and empty symbol) of PAHs (chart a), OCPs (chart b), PBDEs (chart c), and PCBs (chart d) in spiked fish meat cleaned up by SPE cartridge (SC, the white column and the empty circle) and chromatography column (CC, the grey column and the empty triangle), respectively.

    Article Snippet: Considering the cost factor and similar property between PBDEs and PCBs, the PBDEs and PCBs had the same SS and IS, which were 2,2′,3,4,5,5′-hexaCB (13 C12 , 99%, EC-1418-1.2, Cambridge Isotope Laboratories, Inc., USA) (141) and 2,2′,3,3′,4,5,5′,6,6′-nonaCB (13 C12 , 99%, EC-1419-1.2, Cambridge Isotope Laboratories, Inc., USA) (208), respectively.

    Techniques: Standard Deviation, Fluorescence In Situ Hybridization, Chromatography

    The role of hydrological factors (high, stable and low water flow) on total PCDD + PCDF + dl-PCB and total TEQ loads transported along the continuum of the Pilica River at Sulejów, Tomaszów Maz. and Spała river gauge stations.

    Journal: Scientific Reports

    Article Title: An ecohydrological approach to the river contamination by PCDDs, PCDFs and dl-PCBs – concentrations, distribution and removal using phytoremediation techniques

    doi: 10.1038/s41598-019-55973-3

    Figure Lengend Snippet: The role of hydrological factors (high, stable and low water flow) on total PCDD + PCDF + dl-PCB and total TEQ loads transported along the continuum of the Pilica River at Sulejów, Tomaszów Maz. and Spała river gauge stations.

    Article Snippet: PCDD, PCDF and dl-PCB analysis in sediment and soil samples The sample of sediment or soil (2 g) was spiked with isotopically-labeled standards (Cambridge Isotopes Laboratories, USA).

    Techniques: Flow Cytometry

    PCB 126-induced oxidative stress is modulated by green tea extract (GTE) diet supplementation. Plasma F 2 -isoprostane (A) and metabolite (B) levels were measured by HPLC/MS MS to assess in vivo oxidative stress induced by PCB 126 that is potentially mitigated by GTE supplementation. Relative levels of combined F-2 IsoPs, including PGF2α, 8-iso-PGF2α, iPF2α-III, 8-epiPGF2α, 8-isoprostane, and 15-F2t isoprostanes, were determined by averaging the AUC integration values from retention times of 8 and 11.3 minutes (Q1 = 353.144, Q2 =193.1). Additionally, the level of 13,14-dihydro-15-ketoPGF2α, an F-2 IsoP metabolite, was determined by integrating its peak at 11.3 minutes (Q1 = 355.2, Q2 = 311.4). Data are presented as mean±S.E.M. (n=8–10). GTE supplementation significantly decreased oxidative stress induced by PCB 126 treatment (*p

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: PCB 126-induced oxidative stress is modulated by green tea extract (GTE) diet supplementation. Plasma F 2 -isoprostane (A) and metabolite (B) levels were measured by HPLC/MS MS to assess in vivo oxidative stress induced by PCB 126 that is potentially mitigated by GTE supplementation. Relative levels of combined F-2 IsoPs, including PGF2α, 8-iso-PGF2α, iPF2α-III, 8-epiPGF2α, 8-isoprostane, and 15-F2t isoprostanes, were determined by averaging the AUC integration values from retention times of 8 and 11.3 minutes (Q1 = 353.144, Q2 =193.1). Additionally, the level of 13,14-dihydro-15-ketoPGF2α, an F-2 IsoP metabolite, was determined by integrating its peak at 11.3 minutes (Q1 = 355.2, Q2 = 311.4). Data are presented as mean±S.E.M. (n=8–10). GTE supplementation significantly decreased oxidative stress induced by PCB 126 treatment (*p

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: High Performance Liquid Chromatography, Mass Spectrometry, In Vivo

    mRNA expression for AhR (A) and Nrf2 (B) genes in mouse liver samples. GTE diet supplementation leads to significant upregulation of AhR mRNA levels in the presence of PCB 126, thus increasing in vivo toxicant clearance. Nrf2 mRNA levels are significantly increased during PCB 126 insult, although GTE supplementation does not cause statistically significant modulation of PCB toxicity. All values were determined using the relative quantification method (ΔΔCt) as a fold change from control. Data are presented as mean±S.E.M (n=8–10). GTE supplementation significantly increased AhR in the presence of PCB 126 treatment (*p

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: mRNA expression for AhR (A) and Nrf2 (B) genes in mouse liver samples. GTE diet supplementation leads to significant upregulation of AhR mRNA levels in the presence of PCB 126, thus increasing in vivo toxicant clearance. Nrf2 mRNA levels are significantly increased during PCB 126 insult, although GTE supplementation does not cause statistically significant modulation of PCB toxicity. All values were determined using the relative quantification method (ΔΔCt) as a fold change from control. Data are presented as mean±S.E.M (n=8–10). GTE supplementation significantly increased AhR in the presence of PCB 126 treatment (*p

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: Expressing, In Vivo

    Nuclear translocation of Nrf2 in mouse liver samples. Mice fed a 1% GTE-supplemented diet and subsequently exposed to PCB 126 displayed increased Nrf2 activation, as evidenced by increased Nrf2 translocation to the nucleus, compared to mice fed 10% fat control diet and exposed to PCB. Lamin was used as a nuclear fraction housekeeping gene for densitometry quantifications. GTE supplemented mice exposed to PCB showed a trend toward increased nuclear abundance of Nrf2 (*p=0.1, n=4).

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: Nuclear translocation of Nrf2 in mouse liver samples. Mice fed a 1% GTE-supplemented diet and subsequently exposed to PCB 126 displayed increased Nrf2 activation, as evidenced by increased Nrf2 translocation to the nucleus, compared to mice fed 10% fat control diet and exposed to PCB. Lamin was used as a nuclear fraction housekeeping gene for densitometry quantifications. GTE supplemented mice exposed to PCB showed a trend toward increased nuclear abundance of Nrf2 (*p=0.1, n=4).

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: Translocation Assay, Mouse Assay, Activation Assay

    The effect of green tea extract (GTE) diet supplementation on systemic PCB 126 concentration and metabolism. PCB 126 and its hydroxy metabolites were measured in mouse plasma by UFLC/MS MS and normalized to sample volume and internal standard recovery. PCB 126 is heavily metabolized in vivo , as seen by very low levels of parent PCB 126 remaining in plasma samples while its hydroxylated metabolites predominate. GTE supplementation did not significantly modulate systemic PCB or metabolite concentrations. Data are presented as mean±S.E.M (n=5).

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: The effect of green tea extract (GTE) diet supplementation on systemic PCB 126 concentration and metabolism. PCB 126 and its hydroxy metabolites were measured in mouse plasma by UFLC/MS MS and normalized to sample volume and internal standard recovery. PCB 126 is heavily metabolized in vivo , as seen by very low levels of parent PCB 126 remaining in plasma samples while its hydroxylated metabolites predominate. GTE supplementation did not significantly modulate systemic PCB or metabolite concentrations. Data are presented as mean±S.E.M (n=5).

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: Concentration Assay, Mass Spectrometry, In Vivo

    Relative mRNA levels of inflammatory and xenobiotic-related markers, Cyp1A1 (A), Cyp1B1 (B), MCP-1 (C) and CCL3 (D) in mouse liver samples. GTE supplementation led to increased cytochrome P450 (CYP1A1 and CYP1B1) mRNA expression in the presence of both GTE and environmental toxicant (i.e., PCB 126), indicating increased activity for toxicant degradation and/or excretion. MCP-1 and CCL3 inflammatory markers were statistically increased in GTE-supplemented mice liver samples but toxicant-induced inflammatory markers returned to control levels due to GTE supplementation. All values were determined using the relative quantification method (ΔΔCt) as a fold change from control. Data are presented as mean±S.E.M. (*p

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: Relative mRNA levels of inflammatory and xenobiotic-related markers, Cyp1A1 (A), Cyp1B1 (B), MCP-1 (C) and CCL3 (D) in mouse liver samples. GTE supplementation led to increased cytochrome P450 (CYP1A1 and CYP1B1) mRNA expression in the presence of both GTE and environmental toxicant (i.e., PCB 126), indicating increased activity for toxicant degradation and/or excretion. MCP-1 and CCL3 inflammatory markers were statistically increased in GTE-supplemented mice liver samples but toxicant-induced inflammatory markers returned to control levels due to GTE supplementation. All values were determined using the relative quantification method (ΔΔCt) as a fold change from control. Data are presented as mean±S.E.M. (*p

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: Expressing, Activity Assay, Mouse Assay

    GTE supplementation leads to increased antioxidant protein expression in the presence of PCB 126. Protein expression of NQO1 (A) and GSR (B) in mouse liver samples was assessed by Western blot analysis. Protein samples were separated through gel electrophoresis and probed with NQO1 and GSR primary antioxidant-related antibodies. Statistically significant increases in antioxidant protein activity were seen in PCB 126-treated mice that were fed a GTE-supplemented diet. In addition to visualized Western blot comparison to β-actin housekeeping gene, samples were compared to GAPDH housekeeping gene for densitometry quantification to further substantiate findings. GTE supplemented mice exposed to PCB showed a significant increase in protein expression, indicating a strengthened antioxidant response due to GTE supplementation (*p

    Journal: The Journal of nutritional biochemistry

    Article Title: Green tea diet decreases PCB 126-induced oxidative stress in mice by upregulating antioxidant enzymes

    doi: 10.1016/j.jnutbio.2013.10.003

    Figure Lengend Snippet: GTE supplementation leads to increased antioxidant protein expression in the presence of PCB 126. Protein expression of NQO1 (A) and GSR (B) in mouse liver samples was assessed by Western blot analysis. Protein samples were separated through gel electrophoresis and probed with NQO1 and GSR primary antioxidant-related antibodies. Statistically significant increases in antioxidant protein activity were seen in PCB 126-treated mice that were fed a GTE-supplemented diet. In addition to visualized Western blot comparison to β-actin housekeeping gene, samples were compared to GAPDH housekeeping gene for densitometry quantification to further substantiate findings. GTE supplemented mice exposed to PCB showed a significant increase in protein expression, indicating a strengthened antioxidant response due to GTE supplementation (*p

    Article Snippet: PCB 126 and its hydroxy metabolites were isolated from plasma samples (plus 10 μM 13C12-labeled PCB 126 internal standard (IS), Cambridge Isotope Laboratories, Tewksbury, MA) through extraction with acetonitrile and subsequent sonication and centrifugation at 15,000 rpm for 5 min to pellet plasma debris.

    Techniques: Expressing, Western Blot, Nucleic Acid Electrophoresis, Activity Assay, Mouse Assay