pc14 pe6 pgf1 br4 cells  (Thermo Fisher)


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    Trypsin EDTA 0 25 phenol red
    Description:
    Gibco Trypsin EDTA is made from trypsin powder an irradiated mixture of proteases derived from porcine pancreas Due to its digestive strength trypsin is widely used for cell dissociation routine cell culture passaging and primary tissue dissociation The trypsin concentration required for dissociation varies with cell type and experimental requirements See the complete range of Gibco trypsin solutions and recommended dissociation conditions We offer a variety of trypsin formulations and animal origin free TrypLE reagent that feature • Quality testing• Documented traceability• Dual site cGMP ManufacturingThis trypsin solution is modified as follows With• EDTA• Phenol RedThe complete formulation is available Quality testingGibco Trypsin solutions are tested for pH osmolality sterility and performance In addition prior to manufacturing the raw materials are verified for e beam irradiation and tested for endotoxin PPV PCV 1 2 mycoplasma bacterial fungal and viral contamination as well as multiple activity assays ash analysis and moisture analysis Documented traceabilityWe can provide detailed documentation to meet your regulatory needs Gibco Trypsin information available includes lot traceability animal origin certificates lot analyses irradiation certificates a viral inactivation summary and supply chain transparency cGMP manufacturing and quality systemGibco Trypsin is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    Catalog Number:
    25200056
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
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    Structured Review

    Thermo Fisher pc14 pe6 pgf1 br4 cells
    Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of <t>PC14-PE6</t> <t>pGF1</t> <t>Br4</t> metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p
    Gibco Trypsin EDTA is made from trypsin powder an irradiated mixture of proteases derived from porcine pancreas Due to its digestive strength trypsin is widely used for cell dissociation routine cell culture passaging and primary tissue dissociation The trypsin concentration required for dissociation varies with cell type and experimental requirements See the complete range of Gibco trypsin solutions and recommended dissociation conditions We offer a variety of trypsin formulations and animal origin free TrypLE reagent that feature • Quality testing• Documented traceability• Dual site cGMP ManufacturingThis trypsin solution is modified as follows With• EDTA• Phenol RedThe complete formulation is available Quality testingGibco Trypsin solutions are tested for pH osmolality sterility and performance In addition prior to manufacturing the raw materials are verified for e beam irradiation and tested for endotoxin PPV PCV 1 2 mycoplasma bacterial fungal and viral contamination as well as multiple activity assays ash analysis and moisture analysis Documented traceabilityWe can provide detailed documentation to meet your regulatory needs Gibco Trypsin information available includes lot traceability animal origin certificates lot analyses irradiation certificates a viral inactivation summary and supply chain transparency cGMP manufacturing and quality systemGibco Trypsin is manufactured at a cGMP compliant facility located in Grand Island New York The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards
    https://www.bioz.com/result/pc14 pe6 pgf1 br4 cells/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pc14 pe6 pgf1 br4 cells - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Nintedanib and a bi-specific anti-VEGF/Ang2 nanobody selectively prevent brain metastases of lung adenocarcinoma cells"

    Article Title: Nintedanib and a bi-specific anti-VEGF/Ang2 nanobody selectively prevent brain metastases of lung adenocarcinoma cells

    Journal: Clinical & Experimental Metastasis

    doi: 10.1007/s10585-020-10055-x

    Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of PC14-PE6 pGF1 Br4 metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p
    Figure Legend Snippet: Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of PC14-PE6 pGF1 Br4 metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p

    Techniques Used: Magnetic Resonance Imaging, Injection, Mouse Assay, Staining

    Related Articles

    Flow Cytometry:

    Article Title: BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses
    Article Snippet: The absorbance was detected at 450 nm with a microplate reader (ThermoFisher). .. Flow cytometry assayFor GFP reporter assays, PK15 cells were pre-treated with compound for 4 h, and then infected with PRV-GFP (MOI = 0.01) and simultaneously treated with compounds for 36 h. Cells were digested with trypsin-EDTA (25200072, GIBCO), collected by centrifugation and suspended in phosphate-buffered saline (PBS). .. The percentage of GFP positive cells was measured by flow cytometry on a CytoFLEX instrument.

    Infection:

    Article Title: BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses
    Article Snippet: The absorbance was detected at 450 nm with a microplate reader (ThermoFisher). .. Flow cytometry assayFor GFP reporter assays, PK15 cells were pre-treated with compound for 4 h, and then infected with PRV-GFP (MOI = 0.01) and simultaneously treated with compounds for 36 h. Cells were digested with trypsin-EDTA (25200072, GIBCO), collected by centrifugation and suspended in phosphate-buffered saline (PBS). .. The percentage of GFP positive cells was measured by flow cytometry on a CytoFLEX instrument.

    Article Title: Venezuelan Equine Encephalitis Virus nsP3 Phosphorylation Can Be Mediated by IKKβ Kinase Activity and Abrogation of Phosphorylation Inhibits Negative-Strand Synthesis
    Article Snippet: Liquid Chromatography-Mass SpectrometryLC-MS/MS was performed as previously described [ ]. .. Briefly, VEEV TC-83 infected U-87MG or MEF cells were trypsinized (25200056, ThermoFisher) and washed twice with PBS using a centrifugal spin of 1500 rpm for 5 min during each wash. Cellular pellets were lysed using 8 M urea, disulfide bonds were reduced with 1 M DTT and alkylated with iodacetamide. .. Trypsin digestion was performed for 4 h at 37 °C and peptides were eluted with ZipTip purification (Z720070, Millipore,).

    Centrifugation:

    Article Title: BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses
    Article Snippet: The absorbance was detected at 450 nm with a microplate reader (ThermoFisher). .. Flow cytometry assayFor GFP reporter assays, PK15 cells were pre-treated with compound for 4 h, and then infected with PRV-GFP (MOI = 0.01) and simultaneously treated with compounds for 36 h. Cells were digested with trypsin-EDTA (25200072, GIBCO), collected by centrifugation and suspended in phosphate-buffered saline (PBS). .. The percentage of GFP positive cells was measured by flow cytometry on a CytoFLEX instrument.

    Selection:

    Article Title: SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway
    Article Snippet: On day 11, metabolic selection of hiPSC-CMs was started by growing them in a glucose-free RPMI 1640 medium (ThermoFisher Scientific; #11879020) containing 4 mM Sodium DL Lactate solution (Sigma; #L426) for 4 days, replenishing the medium every 2 days. .. Following metabolic selection, purified hiPSC-CMs were trypsinized with 0.25% trypsin-EDTA (ThermoFisher Scientific; #25200114) containing 10 μ g/ml DNase I (StemCell; #7469) and re-plated into 12-well plates coated with 10 μ g/ml human bulk fibronectin (ThermoFisher Scientific; #3560) at a density of 750,000 cells/well. .. The replating medium comprised RPMI 1640 mixed with the insulin-containing B-27 supplement, 2% fetal bovine serum (Sigma; #F0926), and 5 μ M Y-2763 (Tocris: #12543).

    Purification:

    Article Title: SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway
    Article Snippet: On day 11, metabolic selection of hiPSC-CMs was started by growing them in a glucose-free RPMI 1640 medium (ThermoFisher Scientific; #11879020) containing 4 mM Sodium DL Lactate solution (Sigma; #L426) for 4 days, replenishing the medium every 2 days. .. Following metabolic selection, purified hiPSC-CMs were trypsinized with 0.25% trypsin-EDTA (ThermoFisher Scientific; #25200114) containing 10 μ g/ml DNase I (StemCell; #7469) and re-plated into 12-well plates coated with 10 μ g/ml human bulk fibronectin (ThermoFisher Scientific; #3560) at a density of 750,000 cells/well. .. The replating medium comprised RPMI 1640 mixed with the insulin-containing B-27 supplement, 2% fetal bovine serum (Sigma; #F0926), and 5 μ M Y-2763 (Tocris: #12543).

    Concentration Assay:

    Article Title: Nintedanib and a bi-specific anti-VEGF/Ang2 nanobody selectively prevent brain metastases of lung adenocarcinoma cells
    Article Snippet: .. Briefly, PC14-PE6 pGF1 Br4 cells were trypsinized (Gibco, Life Science Technologies, cat. no.: 25200-056), washed, counted and resuspended in PBS (cat. no: D8537, Sigma Life Sciences) in a final concentration of 5 × 106 cells/mL. .. 8-week-old male NOD/SCID mice were anesthetized with a xylazine-ketamine-injection (intraperitoneal injection of 100 µL NaCl-solution containing 1 mg/mL of xylazine (Rompun 2%, 20 mg/mL, Bayer) and 15 mg/mL ketamine (100 mg/mL, WDT)).

    Produced:

    Article Title: Genome-wide analysis of DNA G-quadruplex motifs across 37 species provides insights into G4 evolution
    Article Snippet: Protoplasts from S. cerevisiae were produced according to kit instructions for protoplast preparation (BB36301, BestBio, Shanghai, China). .. D. rerio cells and P. sinensis cells were produced from adult fish and Pelodiscus by using Trypsin-EDTA (25200072, Thermo Scientific, MA, USA) digestion. .. The S. cerevisiae protoplasts, D. rerio cells, and P. sinensis cells were fixed on poly-L-lysine (P4707, Sigma-Aldrich)-coated glass coverslips in a 12-well plate.

    Fluorescence In Situ Hybridization:

    Article Title: Genome-wide analysis of DNA G-quadruplex motifs across 37 species provides insights into G4 evolution
    Article Snippet: Protoplasts from S. cerevisiae were produced according to kit instructions for protoplast preparation (BB36301, BestBio, Shanghai, China). .. D. rerio cells and P. sinensis cells were produced from adult fish and Pelodiscus by using Trypsin-EDTA (25200072, Thermo Scientific, MA, USA) digestion. .. The S. cerevisiae protoplasts, D. rerio cells, and P. sinensis cells were fixed on poly-L-lysine (P4707, Sigma-Aldrich)-coated glass coverslips in a 12-well plate.

    other:

    Article Title: Contact and Encirclement of Glioma Cells In Vitro Is an Intrinsic Behavior of a Clonal Human Neural Stem Cell Line
    Article Snippet: Equal volumes of the HB1.F3.eGFP/target cell mixture and 0.3% Puramatrix working solution were then mixed together, and 150 µl was pipetted using a clipped 200 µl tip into 1 ml growth medium per well of a 24-well plate to a final cell density of 1.5×105 total cells/ml.

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  • 97
    Thermo Fisher pc14 pe6 pgf1 br4 cells
    Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of <t>PC14-PE6</t> <t>pGF1</t> <t>Br4</t> metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p
    Pc14 Pe6 Pgf1 Br4 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc14 pe6 pgf1 br4 cells/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pc14 pe6 pgf1 br4 cells - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of PC14-PE6 pGF1 Br4 metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p

    Journal: Clinical & Experimental Metastasis

    Article Title: Nintedanib and a bi-specific anti-VEGF/Ang2 nanobody selectively prevent brain metastases of lung adenocarcinoma cells

    doi: 10.1007/s10585-020-10055-x

    Figure Lengend Snippet: Nintedanib and anti-VEGF/Ang2 nanobody prevent brain metastases formation. 9.4 T MRI after Gadolinium contrast administration was performed on day 26 after intracardial tumor cell injection. a Bar chart illustrating the reduced percentage of mice with metastases, detectable in cMRI. b Representative T1-w cMRI images depicting larger intracranial metastases in the control groups, indicated by arrowheads. scale bar = 2 mm. c Scatter plots showing the reduction of number and volume of cranial metastases in cMRI. d Scatter plots depicting the number and volume of meningeal metastases in cMRI e Quantification demonstrating a higher histological tumor–tissue ratio in control mice at their time of death. f Representative histological slices with higher number and size of PC14-PE6 pGF1 Br4 metastases in the control group. Fluorescent staining: DAPI (blue) = nucleus, GFP (green) = PC14-PE6 tumor cells, Alexa Flour 546 (orange) = collagen-IV positive vascular basement membrane. Arrows indicate GFP-positive metastatic lesions. Scale bar = 1000 µm. Mean values with standard errors of the mean are shown. *p

    Article Snippet: Briefly, PC14-PE6 pGF1 Br4 cells were trypsinized (Gibco, Life Science Technologies, cat. no.: 25200-056), washed, counted and resuspended in PBS (cat. no: D8537, Sigma Life Sciences) in a final concentration of 5 × 106 cells/mL.

    Techniques: Magnetic Resonance Imaging, Injection, Mouse Assay, Staining