Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: Low-level laser (LLL) exposure improves cisplatin-induced cytotoxicity and apoptosis in PC12 cells. Cisplatin-induced reduction in cell viability (A) and increase in cytotoxicity (B) were markedly attenuated by LLL treatment at 5 J/cm 2 . (C) Apoptosis triggered by cisplatin was reduced by LLL irradiation, as determined by TUNEL assay with flow cytometry. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Irradiation, TUNEL Assay, Flow Cytometry, Control

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL enhances the cisplatin-induced autophagy in an AMPK-dependent manner. Representative Western blot images (A) and corresponding densitometric bar graphs (B, C, D) of Beclin-1, LC3-I/II, and p62 in PC12 cells exposed to cisplatin, either alone, in combination with 5 J/cm 2 LLL, or under AMPK silencing conditions, are presented. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Western Blot, Control

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL reduces oxidative stress and changes in mitochondrial membrane potential evoked by cisplatin through AMPK-mediated autophagy. Representative measurements of (A) malondialdehyde (MDA) and (B) mitochondrial superoxide levels in PC12 cells treated with cisplatin, and further subjected to LLL, AMPK knockdown, or rapamycin-induced autophagy. Results were expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group. Mitochondrial membrane potential was assessed using JC-1 staining. Fluorescence intensities in the FL-1 (green) and FL-2 (red) channels were measured by flow cytometry and quantified, with the results presented as a bar graph (C). Experimental conditions included treatment with chloroquine (an autophagy inhibitor) and MitoTEMPO (a mitochondria-targeted antioxidant), either alone or in combination with cisplatin and/or LLL.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Membrane, Knockdown, Control, Staining, Fluorescence, Flow Cytometry

Journal: ACS Omega

Article Title: Low-Level Laser Mitigates Cisplatin-Induced Oxidative Stress and Apoptosis via AMPK-Mediated Autophagy in PC12 Cells

doi: 10.1021/acsomega.5c12039

Figure Lengend Snippet: LLL inhibits cisplatin-induced apoptosis through the activation of AMPK. Representative Western blot images (A) and corresponding densitometric bar graphs (B-E) of p53, Bax, Bcl-2, and cytosolic cytochrome c in PC12 cells exposed to cisplatin, either alone, in combination with 5 J/cm 2 LLL, or under AMPK silencing conditions, were presented. Results are expressed as means ± SD of three independent experiments. * Indicates p < 0.05 compared with the control group. # Indicates p < 0.05 compared to the cisplatin group. & Indicates p < 0.05 compared to the cisplatin + LLL group.

Article Snippet: Rat pheochromocytoma PC12 cells were obtained from the American Type Culture Collection (ATCC) and cultured in DMEM supplemented with 10% heat-inactivated horse serum, 5% FBS, 1% PS, and 1% l -glutamine.

Techniques: Activation Assay, Western Blot, Control

Journal: Neural Regeneration Research

Article Title: Polydopamine-coupled NT 3 -derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration

doi: 10.4103/NRR.NRR-D-24-01544

Figure Lengend Snippet: Effect of PLGA/CNT-PDA-NT 3 on PC12 cell growth. (A) Schematic diagram of the mechanism through which neural conduits promote PC12 cell axon growth. (B) CLSM images of PC12 cells. DAPI staining is shown in blue, and phalloidin staining is shown in green (scale bar: 200 μm). (C) Average PC12 cell axon length ( n = 6). (D) Quantitative analysis of immunofluorescence intensity ( n = 6). (E–G) Polarity histogram of the directional distribution of PC12 cell axons on scaffolds. (H–J) Relative gene expression levels of neuronal markers ( NF200 , GAP43 , and Tubb3 ), as determined by quantitative reverse transcription-polymerase chain reaction. Data are expressed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, vs . PLGA/CNT; # P < 0.05, ## P < 0.01, vs . PLGA/CNT-PDA (one-way analysis of variance followed by Tukey’s post hoc test). CLSM: Confocal laser scanning microscopy; CNT: carbon nanotube; GAP43: growth associated protein 43; NF200: neurofilament-200; NT 3 : neurotrophin-3; PDA: polydopamine; PLGA: polylactic-glycolic acid.

Article Snippet: RSC96, PC12, and RAW 264.7 (CL-0190, Procell Life Science & Technology Co., Ltd, Wuhan, China) cells were seeded into a 96-well plate at a density of 8 × 10 3 cells per well.

Techniques: Staining, Immunofluorescence, Gene Expression, Reverse Transcription, Polymerase Chain Reaction, Confocal Laser Scanning Microscopy

Journal: Neural Regeneration Research

Article Title: Polydopamine-coupled NT 3 -derived oriented conductive scaffolds with immunomodulatory properties accelerate peripheral nerve regeneration

doi: 10.4103/NRR.NRR-D-24-01544

Figure Lengend Snippet: Antioxidant and immunomodulatory effects of PLGA/CNT-PDA-NT 3 . (A) Schematic diagram of ROS scavenging and macrophage phenotype induction by the fibrous scaffolds. (B) DPPH test of antioxidant capacity ( n = 3). (C) ROS probe test (scale bar: 200 μm). (D) Quantitative analysis of ROS fluorescence intensity ( n = 3). (E–G) Survival rates of RSC96, PC12, and RAW264.7 cells exposed to 100 μM H 2 O 2 , as measured by Cell Counting Kit-8 ( n = 3). (H) Fluorescence images showing the cytoskeletons and nuclei of RAW 264.7 macrophages cultured for 24 hours under normal conditions or on fibrous scaffolds. Arrows indicate cells with M2 morphology (scale bar: 50 μm). (I–L) TNF-α , iNOS , IL-6 , IL-10 , and mRNA expression in RAW264.7 cells, as determined by quantitative reverse transcription-polymerase chain reaction. Data are expressed as mean ± SD ( n = 3). * P < 0.05, ** P < 0.01, vs . PLGA/CNT; # P < 0.05, ## P < 0.01, vs . PLGA/CNT-PDA; $$ P < 0.01, vs . control /H 2 O 2 group (one-way analysis of variance followed by Tukey’s post hoc test). CNT: Carbon nanotube; IL: interleukin; iNOS: inducible nitric oxide synthase; NT 3 : neurotrophin-3; PDA: polydopamine; PLGA: polylactic-glycolic acid; ROS: reactive oxygen species; TNF-α: tumor necrosis factor-α.

Article Snippet: RSC96, PC12, and RAW 264.7 (CL-0190, Procell Life Science & Technology Co., Ltd, Wuhan, China) cells were seeded into a 96-well plate at a density of 8 × 10 3 cells per well.

Techniques: Fluorescence, Cell Counting, Cell Culture, Expressing, Reverse Transcription, Polymerase Chain Reaction, Control