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rocky mountain labs pbvs2 himar1 c9
Plasmid maps of pCR2.1-P1196- <t>Himar1</t> C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.
Pbvs2 Himar1 C9, supplied by rocky mountain labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pbvs2 himar1 c9 - by Bioz Stars, 2020-08
85/100 stars

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1) Product Images from "Characterization of a Coxiella burnetii ftsZ Mutant Generated by Himar1 Transposon Mutagenesis ▿ Transposon Mutagenesis ▿ †"

Article Title: Characterization of a Coxiella burnetii ftsZ Mutant Generated by Himar1 Transposon Mutagenesis ▿ Transposon Mutagenesis ▿ †

Journal: Journal of Bacteriology

doi: 10.1128/JB.01580-08

Plasmid maps of pCR2.1-P1196- Himar1 C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.
Figure Legend Snippet: Plasmid maps of pCR2.1-P1196- Himar1 C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.

Techniques Used: Plasmid Preparation, Expressing, Variant Assay, Clone Assay, Sequencing

Related Articles

Clone Assay:

Article Title: Characterization of a Coxiella burnetii ftsZ Mutant Generated by Himar1 Transposon Mutagenesis ▿ Transposon Mutagenesis ▿ †
Article Snippet: .. Himar1 C9 from pBVS2- Himar1 C9 (generously provided by P. Stewart, Rocky Mountain Laboratories) was amplified by using Accuprime Taq DNA polymerase and the primers Himar1 C9-NdeI-F and Himar1 C9-HindIII-R and then cloned into pCR2.1-Topo. .. P1169 was amplified from C. burnetii genomic DNA (gDNA) by using Accuprime Taq and the primers P1169-PstI-F and P1169-NdeI-R and then cloned into the pCR2.1-topo vector, creating pCR2.1-P1169.

Amplification:

Article Title: Characterization of a Coxiella burnetii ftsZ Mutant Generated by Himar1 Transposon Mutagenesis ▿ Transposon Mutagenesis ▿ †
Article Snippet: .. Himar1 C9 from pBVS2- Himar1 C9 (generously provided by P. Stewart, Rocky Mountain Laboratories) was amplified by using Accuprime Taq DNA polymerase and the primers Himar1 C9-NdeI-F and Himar1 C9-HindIII-R and then cloned into pCR2.1-Topo. .. P1169 was amplified from C. burnetii genomic DNA (gDNA) by using Accuprime Taq and the primers P1169-PstI-F and P1169-NdeI-R and then cloned into the pCR2.1-topo vector, creating pCR2.1-P1169.

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    rocky mountain labs pbvs2 himar1 c9
    Plasmid maps of pCR2.1-P1196- <t>Himar1</t> C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.
    Pbvs2 Himar1 C9, supplied by rocky mountain labs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbvs2 himar1 c9/product/rocky mountain labs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbvs2 himar1 c9 - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

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    Plasmid maps of pCR2.1-P1196- Himar1 C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.

    Journal: Journal of Bacteriology

    Article Title: Characterization of a Coxiella burnetii ftsZ Mutant Generated by Himar1 Transposon Mutagenesis ▿ Transposon Mutagenesis ▿ †

    doi: 10.1128/JB.01580-08

    Figure Lengend Snippet: Plasmid maps of pCR2.1-P1196- Himar1 C9 and p1898-Tn. (A) In pCR2.1-P1196- Himar1 C9, the promoter from CBU1169 (P1169) drives expression of the C9 variant of the Himar1 transposase. (B) In p1898-Tn, the Himar1 transposon contains CAT and mCherry genes expressed as a single transcriptional unit from P1169. Also contained within the Himar1 ITRs is a ColE1 origin of replication that allows rescue cloning of the Tn in E. coli . Outside of the transposon is the coding sequence of CBU1898.

    Article Snippet: Himar1 C9 from pBVS2- Himar1 C9 (generously provided by P. Stewart, Rocky Mountain Laboratories) was amplified by using Accuprime Taq DNA polymerase and the primers Himar1 C9-NdeI-F and Himar1 C9-HindIII-R and then cloned into pCR2.1-Topo.

    Techniques: Plasmid Preparation, Expressing, Variant Assay, Clone Assay, Sequencing