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SouthernBiotech pbst
The <t>fibronectin-binding</t> ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl <t>PBST</t> was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p
Pbst, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/SouthernBiotech
Average 93 stars, based on 18 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
93/100 stars

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1) Product Images from "Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin"

Article Title: Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin

Journal: Scientific Reports

doi: 10.1038/s41598-017-00121-y

The fibronectin-binding ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl PBST was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p
Figure Legend Snippet: The fibronectin-binding ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl PBST was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p

Techniques Used: Binding Assay, Activity Assay, Indirect ELISA, Enzyme-linked Immunosorbent Assay, Incubation

Related Articles

Labeling:

Article Title: Early-Life Antibiotics Attenuate Regulatory T Cell Generation and Increase the Severity of Murine House Dust Mite-Induced Asthma
Article Snippet: .. Plates were washed eight times with PBST and labeled with Biotin-SP-conjugated goat anti- mouse IgE (Southern Biotech, Birmingham, AL; Cat #1110-08) diluted 1:5000 in blocking buffer for one hour at room temperature. .. Plates were then washed six times and incubated with streptavidin HRP (BD Biosciences, San Jose, CA; Cat #554066) diluted 1:1000 in blocking buffer at room temperature for thirty minutes.

Incubation:

Article Title: Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin
Article Snippet: .. The reactions proceeded at 37 °C for 1 h. After the wells were washed, the bound fibronectin was detected by incubation with anti-fibronectin mAb (Sigma) (1:1000 in PBST) at 37 °C for 1 h, and then with HRP-conjugated goat anti-mouse IgG antibody (1:5,000 in PBST) (Southern Biotech) at 37 °C for 45 min. .. The reaction was developed with the substrate tetramethylbenzidine/H2 O2 (Wuhan Keqian Animal Biological Products Co., Ltd, Wuhan, China) and stopped with hydrogen fluoride.

Article Title: Lung cancer induced in mice by the envelope protein of jaagsiekte sheep retrovirus (JSRV) closely resembles lung cancer in sheep infected with JSRV
Article Snippet: .. Antibodies (tissue culture supernatants) were reacted with antigens for 1 h at 37°C, rinsed with PBST, and incubated for an additional hour with a 1:10,000 dilution of HRP conjugated goat anti-mouse IgG (γ chain) (Southern Biotech). ..

Article Title: NOP14 inhibits melanoma proliferation and metastasis by regulating Wnt/β-catenin signaling pathway
Article Snippet: .. After washing thrice with PBST, the membrane was incubated with 1:10,000 dilution of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG H & L secondary antibodies (Southern Biotech, USA). .. The membrane was rinsed, and protein bands were visualized using an enhanced chemiluminescence detection kit (Thermo Scientific).

Article Title: SAF-A Has a Role in Transcriptional Regulation of Oct4 in ES Cells Through Promoter Binding
Article Snippet: .. After washing with PBST, blots were incubated for 1 h with secondary antibodies (AP conjugated goat antimouse IgM + IgG + IgA (H + L) and AP conjugated goat antirabbit IgM + IgG (H + L chain specific) (1:1000; SouthernBiotech, Birmkingham, AL). .. Visualization of proteins was done with NBT/BCIP (Promega, Madison, WI).

Article Title: Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity
Article Snippet: .. Briefly, the sections were rehydrated, blocked with 10% donkey serum at room temperature for 30 minutes or longer, incubated with primary antibodies in PBS containing 0.1% Triton X-100 (PBST) overnight at 4° C, washed in PBST for three times at room temperature, incubated with appropriate fluorescent secondary antibodies at room temperature for 1 hour or longer, and mounted in Fluro-G (SouthernBiotech) for imaging. .. An inverted Olympus IX71 microscope or Zeiss confocal microscope was used for imaging.

Article Title: Fgf20 Is Required for Sensory Epithelial Specification in the Developing Cochlea
Article Snippet: .. The slides were then incubated with 10% fetal bovine serum and 2% nonfat dry milk in PBS/0.1% Triton X-100 (PBST) for 30 min. After an overnight incubation with the primary antibody (rabbit anti-Myo6 or mouse anti-p27 kip1 ; BD Transduction Laboratories) at 1:300 dilution at 4°C, the sections were rinsed with PBST, incubated for 90 min with a fluorescent-conjugated secondary antibody, rinsed with PBST, and coverslipped in Fluoromount G (Southern Biotechnology). .. Images were captured on a Zeiss Axioplan microscope using a SPOT CCD camera and processed using Adobe Photoshop.

Imaging:

Article Title: Homozygous frameshift mutations in FAT1 cause a syndrome characterized by colobomatous-microphthalmia, ptosis, nephropathy and syndactyly
Article Snippet: .. Cells were then washed in PBST before mounting with Fluoromount-G® (SouthernBiotech, Birmingham, AL, USA) imaging. .. Primary antibodies used were FAT1 (Abcam: ab190242 and EMD Millipore: MABC612), YAP/TAZ (Cell Signaling Technology: 8418), FITC-tagged ZO-1 (Invitrogen: 33–9111).

Article Title: Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity
Article Snippet: .. Briefly, the sections were rehydrated, blocked with 10% donkey serum at room temperature for 30 minutes or longer, incubated with primary antibodies in PBS containing 0.1% Triton X-100 (PBST) overnight at 4° C, washed in PBST for three times at room temperature, incubated with appropriate fluorescent secondary antibodies at room temperature for 1 hour or longer, and mounted in Fluro-G (SouthernBiotech) for imaging. .. An inverted Olympus IX71 microscope or Zeiss confocal microscope was used for imaging.

Blocking Assay:

Article Title: Early-Life Antibiotics Attenuate Regulatory T Cell Generation and Increase the Severity of Murine House Dust Mite-Induced Asthma
Article Snippet: .. Plates were washed eight times with PBST and labeled with Biotin-SP-conjugated goat anti- mouse IgE (Southern Biotech, Birmingham, AL; Cat #1110-08) diluted 1:5000 in blocking buffer for one hour at room temperature. .. Plates were then washed six times and incubated with streptavidin HRP (BD Biosciences, San Jose, CA; Cat #554066) diluted 1:1000 in blocking buffer at room temperature for thirty minutes.

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  • 93
    SouthernBiotech pbst
    The <t>fibronectin-binding</t> ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl <t>PBST</t> was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p
    Pbst, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/SouthernBiotech
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    SouthernBiotech bsa pbst
    The <t>fibronectin-binding</t> ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl <t>PBST</t> was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p
    Bsa Pbst, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa pbst/product/SouthernBiotech
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bsa pbst - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    Image Search Results


    The fibronectin-binding ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl PBST was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p

    Journal: Scientific Reports

    Article Title: Mycoplasma bovis NADH oxidase functions as both a NADH oxidizing and O2 reducing enzyme and an adhesin

    doi: 10.1038/s41598-017-00121-y

    Figure Lengend Snippet: The fibronectin-binding ability of rNOX. The rNOX, rPGK, and E. coli total protein were applied to examine the fibronectin activity with indirect ELISA. Different amounts of proteins were coated in the 96 wells of ELISA plate. After washing and blocked with 5% BSA, 200 ng fibronectin in 100 μl PBST was added into each well and incubated for 1 h. And then the anti-fibronectin mAb and goat anti-mouse antibodies were sequentially overlaid to detect the binding activity. ***Represents very significant difference ( p

    Article Snippet: The reactions proceeded at 37 °C for 1 h. After the wells were washed, the bound fibronectin was detected by incubation with anti-fibronectin mAb (Sigma) (1:1000 in PBST) at 37 °C for 1 h, and then with HRP-conjugated goat anti-mouse IgG antibody (1:5,000 in PBST) (Southern Biotech) at 37 °C for 45 min.

    Techniques: Binding Assay, Activity Assay, Indirect ELISA, Enzyme-linked Immunosorbent Assay, Incubation