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Roche pbst
Pbst, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/Roche
Average 92 stars, based on 287 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Induction of systemic and mucosal immunity against methicillin-resistant Staphylococcus aureus infection by a novel nanoemulsion adjuvant vaccine
Article Snippet: .. Briefly, ELISA plates (Costar, 42592; Corning, Corning, NY, USA) were washed five times with PBST and incubated for 60 minutes at 37°C with 1% BSA (m/v; Roche, Basel, Switzerland) after coating each well with 1 μg antigen protein at 4°C for 12 hours. .. One hundred microliters of HRP-conjugated goat anti-mouse IgG (Zhongshan Gold Bridge of Beijing, 112,971, 1:5,000), IgG1 (Bethyl, A90-105P, 1:5,000), IgG2a (Bethyl, A90-107P, 1:5,000), IgG2b (Bethyl, A90-109P, 1:5,000), or IgA (Bethyl, A90-103P, 1:5,000) secondary antibody was added to each plate.

Western Blot:

Article Title: Methamphetamine-mediated endoplasmic reticulum (ER) stress induces type-1 programmed cell death in astrocytes via ATF6, IRE1α and PERK pathways
Article Snippet: .. Finally the membranes were washed again 5 times for 5 minutes each with PBST and target protein bands were visualized using BM chemiluminescence western blotting substrate (POD, Roche Applied Sciences; Indianapolis, IN, USA). .. The band intensities were quantified with FluorChem HD2 software (Alpha Innotech, San Leandro, CA, USA).

Incubation:

Article Title: Stretching Morphogenesis of the Roof Plate and Formation of the Central Canal
Article Snippet: .. Whole-mount Immunofluorescent Histochemistry Embryos were fixed in Histochoice Tissue Fixative MB (Amresco, USA) for 2 hours at room temperature, washed 3 times for 15 min in PBST (1 x PBS, 0.1% Tween-20) and permeabilized in 0.1% Triton X-100 in 0.1% sodium citrate for 30 min. Then embryos were incubated for 2 hours in 5% Blocking Reagent (Roche) in MAB (150 mM maleic acid, pH 7.5, 100 mM NaCl, 0.1% Tween-20). ..

Article Title: Surfing the vegetal pole in a small population: extracellular vertical transmission of an 'intracellular' deep-sea clam symbiont
Article Snippet: .. After washing in PBST twice for 20 min, the sections were incubated in 0.5% blocking reagent (Roche) in PBST for 30 min and then incubated overnight in a 1/2000 volume of anti-DIG-AP (Roche) in PBST containing 0.5% blocking reagent at 4°C. .. The sections were washed three times in PBST for 20 min and once in TMNT buffer, and then incubated in 0.1 × NBT/BCIP solution (Roche) including 2 mM levamisole in TMNT.

Article Title: Induction of systemic and mucosal immunity against methicillin-resistant Staphylococcus aureus infection by a novel nanoemulsion adjuvant vaccine
Article Snippet: .. Briefly, ELISA plates (Costar, 42592; Corning, Corning, NY, USA) were washed five times with PBST and incubated for 60 minutes at 37°C with 1% BSA (m/v; Roche, Basel, Switzerland) after coating each well with 1 μg antigen protein at 4°C for 12 hours. .. One hundred microliters of HRP-conjugated goat anti-mouse IgG (Zhongshan Gold Bridge of Beijing, 112,971, 1:5,000), IgG1 (Bethyl, A90-105P, 1:5,000), IgG2a (Bethyl, A90-107P, 1:5,000), IgG2b (Bethyl, A90-109P, 1:5,000), or IgA (Bethyl, A90-103P, 1:5,000) secondary antibody was added to each plate.

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. After PBST (PBS + 0.1% tween 20) washes for 10 min four times, embryos were incubated with anti-rabbit Alexa-488 (1:200, Thermal Fisher Scientific) at room temperature for 3 h. Embryos were then washed with PBST and blocked with 2% blocking reagent for 1 h before incubation with anti-Digoxigenin-POD (1:500, Roche) diluted in 2% blocking reagent at 4°C overnight. .. After PBST washes, embryos were incubated with TSA-Cy3 (1:50, Perkin Elmer) diluted in Amplification buffer at 28°C for 1 h. Embryos were then washed with PBST, post fixation with 4% paraformaldehyde for 20 min, PBST washes and stored in 80% glycerol at 4°C.

Article Title: Behavioral and transcriptomic analysis of Trem2-null mice: not all knockout mice are created equal
Article Snippet: .. Following three washes with PBST, samples and standards diluted in 0.25% BSA/PBST supplemented with protease inhibitors (Roche, Indianapolis, IN) were incubated overnight at 4 °C. .. Plates were washed three times with PBST and incubated with 1 μg/ml rat monoclonal anti-mouse TREM2 (R & D Systems, Minneapolis, MN) for 1 h at RT.

Blocking Assay:

Article Title: Stretching Morphogenesis of the Roof Plate and Formation of the Central Canal
Article Snippet: .. Whole-mount Immunofluorescent Histochemistry Embryos were fixed in Histochoice Tissue Fixative MB (Amresco, USA) for 2 hours at room temperature, washed 3 times for 15 min in PBST (1 x PBS, 0.1% Tween-20) and permeabilized in 0.1% Triton X-100 in 0.1% sodium citrate for 30 min. Then embryos were incubated for 2 hours in 5% Blocking Reagent (Roche) in MAB (150 mM maleic acid, pH 7.5, 100 mM NaCl, 0.1% Tween-20). ..

Article Title: Surfing the vegetal pole in a small population: extracellular vertical transmission of an 'intracellular' deep-sea clam symbiont
Article Snippet: .. After washing in PBST twice for 20 min, the sections were incubated in 0.5% blocking reagent (Roche) in PBST for 30 min and then incubated overnight in a 1/2000 volume of anti-DIG-AP (Roche) in PBST containing 0.5% blocking reagent at 4°C. .. The sections were washed three times in PBST for 20 min and once in TMNT buffer, and then incubated in 0.1 × NBT/BCIP solution (Roche) including 2 mM levamisole in TMNT.

Article Title: System-wide identification of wild-type SUMO-2 conjugation sites
Article Snippet: .. Cells were washed twice with PBS, and permeabilized with 0.1% Triton X-100 for 10 min, washed with PBST and blocked using TNB (100 mM TRIS pH 7.5, 150 mM NaCl and 0.5% Blocking Reagent (Roche)) for 30 min. .. Cells were incubated with Mouse α SUMO-2/3 antibody (ab81371, Abcam, 1:500) in TNB for 1 h. Cells were washed five times with PBST, and indicated with secondary antibody (Goat α Mouse Alexa 488 (Invitrogen), 1:500) in TNB for 1 h. Subsequently, cells were washed five times with PBST and dehydrated using alcohol, prior to embedding them in Citifluor (Agar Scientific) containing 400 ng per μl DAPI (Sigma) and sealing the slides with nail varnish.

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: .. After PBST (PBS + 0.1% tween 20) washes for 10 min four times, embryos were incubated with anti-rabbit Alexa-488 (1:200, Thermal Fisher Scientific) at room temperature for 3 h. Embryos were then washed with PBST and blocked with 2% blocking reagent for 1 h before incubation with anti-Digoxigenin-POD (1:500, Roche) diluted in 2% blocking reagent at 4°C overnight. .. After PBST washes, embryos were incubated with TSA-Cy3 (1:50, Perkin Elmer) diluted in Amplification buffer at 28°C for 1 h. Embryos were then washed with PBST, post fixation with 4% paraformaldehyde for 20 min, PBST washes and stored in 80% glycerol at 4°C.

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  • pbst  (Roche)
    92
    Roche pbst
    Pbst, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 288 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Roche
    Average 92 stars, based on 288 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    88
    Roche triton x100 pbst
    Triton X100 Pbst, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x100 pbst/product/Roche
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    triton x100 pbst - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

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