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FUJIFILM pbst
Pbst, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/FUJIFILM
Average 92 stars, based on 81 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
92/100 stars

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Incubation:

Article Title: IgY‐binding peptide screened from a random peptide library as a ligand for IgY purification) IgY‐binding peptide screened from a random peptide library as a ligand for IgY purification
Article Snippet: .. After 1 h incubation, the wells were washed five times with PBST, and binding was detected with tetramethylbenzidine (Wako Pure Chemical, Osaka, Japan) reagent. .. Finally, 1 M hydrochloric acid (40 μL/well) was added to stop the reaction, and binding was measured by the absorbance at 450 nm in a microplate reader (680XR; Bio‐Rad, Hercules, CA, USA).

Article Title: Induction of Cell Cycle Arrest and Apoptosis by Physcion, an Anthraquinone Isolated From Rhubarb (Rhizomes of Rheum tanguticum), in MDA-MB-231 Human Breast Cancer Cells
Article Snippet: .. After washing, membranes were incubated with horseradish peroxidas (HRP)-conjugated anti-mouse immunoglobulin G diluted 1 : 2,000 in PBST for 2 hours at room temperature, washed three times for 5 minutes with PBST, and visualized by HRP-chemiluminescent detection kit (Lab Frontier, Seoul, Korea) using LAS-3000 Imager (Fuji Film Corp., Tokyo, Japan). ..

Article Title: Identification of candidates for interacting partners of the tail domain of DcNMCP1, a major component of the Daucus carota nuclear lamina-like structure
Article Snippet: .. The membrane was incubated at 30°C for 1 h in PBS containing 1% (w/v) Block Ace (DS Pharma Biomedical, UKB40) and 1% (w/v) bovine serum albumin, washed twice with PBST (PBS supplemented with 0.2% (v/v) Tween 20), incubated at 30°C for 3 h in PBST containing either GST or GST-T975–1053 as probe (probe concentrations are indicated in ), washed 3 times with PBST, incubated at 25°C for 3 h in PBST containing Anti-GST, Monoclonal Antibody, Peroxidase Conjugated (Wako, 011–21891), and washed 3 times with PBST. .. Signals were detected using the Chemi-Lumi One L chemiluminescent substrate (Nacalai Tesque, 07880), and the LumiVision Pro 400EX imager (AISIN).

Article Title: Specificity of two monoclonal antibodies against a synthetic glycopeptide, an analogue to the hypo-galactosylated IgA1 hinge region
Article Snippet: .. Following a further 2 h incubation at 25 °C, the wells were washed 5 times with 250 µl of PBST, and the signal was detected with 100 µl of O -phenylenediamine (0.5 mg/ml Wako Pure Chemical Industries, LTD., Osaka, Japan)—H2 O2 (0.1 %) in 0.1 M citrate phosphate buffer. ..

Article Title: Segmented Iba1-Positive Processes of Microglia in Autism Model Marmosets
Article Snippet: .. After incubation in 1% bovine serum albumin (BSA; Sigma, St. Louis, MO, United States) in PBST for 2 h, sections were further incubated in rabbit anti-Iba1 primary antibody (Wako, Osaka, Japan) diluted 1:1,000 in PBST containing 1% BSA at 4°C overnight. .. After three washes with PBST, sections were subjected to incubation for an additional 2 h with biotinylated anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, United States) diluted 1:200 in PBST containing 1% BSA at room temperature.

Article Title: Ser46-Phosphorylated MARCKS Is a Marker of Neurite Degeneration at the Pre-aggregation Stage in PD/DLB Pathology
Article Snippet: .. The sections were washed twice for 5 min each with PBST (PBS containing 0.1% Tween 20), treated with PBS containing 0.5% Triton X-100 for 20 min, and washed three times with PBST for 5 min. After blocking (10% FBS for 30 min at 37°C), the sections were incubated sequentially with PBS containing 2% FBS and 0.1% Triton X-100, primary antibodies [mouse anti–phospho-α-Syn (Ser129; 1:2000, Wako, 015-25191) for 60 min at 37°C; mouse anti–α-Syn (1:1000, Abcam, ab27766) for 12 h at 4°C; rabbit anti–phospho-MARCKS (Ser46; 1:1000, GL Biochem) for 120 min at 37°C; or mouse anti-ubiquitin antibody (1:1000, Cell Signaling Technology, 3936S); rabbit anti–phospho-ERK1/2 detecting mouse Thr203/Tyr205-ERK1/Thr183/Tyr185-ERK2 and human Thr202/Tyr204-ERK1/Thr185/Tyr187-ERK2 (1:250, Cell Signaling Technology, 4370), mouse anti-MAP2 (1:100, Santa Cruz, sc-32791), or mouse anti-GFAP (1:2000, Sigma, C9205) for 12 h at 4°C], and secondary antibodies [Alexa Fluor 488–labeled anti-mouse IgG (1:1000, Invitrogen) or Cy3-labeled anti-rabbit IgG (1:500, Jackson ImmunoResearch) for 60 min at RT]. pSer46-MARCKS antibody was generated, and the quality was examined, as reported previously ( ). .. For double-labeling of pSer129-α-Syn and ubiquitin, anti–pSer129-α-Syn antibody was labeled with the Zenon Alexa Fluor 488 mouse IgG1 labeling kit (Z-25002, Invitrogen).

Article Title: B-cell repertoire specific for an unfolded self-determinant of mouse lysozyme escape tolerance and dominantly participate in the autoantibody response
Article Snippet: .. After washing with PBST, the final reaction was visualized by incubation with p -nitrophenyl phosphate (Wako) in 0·1 m carbonate buffer (pH 9·6) containing 1 m m MgCl2 . ..

Binding Assay:

Article Title: IgY‐binding peptide screened from a random peptide library as a ligand for IgY purification) IgY‐binding peptide screened from a random peptide library as a ligand for IgY purification
Article Snippet: .. After 1 h incubation, the wells were washed five times with PBST, and binding was detected with tetramethylbenzidine (Wako Pure Chemical, Osaka, Japan) reagent. .. Finally, 1 M hydrochloric acid (40 μL/well) was added to stop the reaction, and binding was measured by the absorbance at 450 nm in a microplate reader (680XR; Bio‐Rad, Hercules, CA, USA).

Generated:

Article Title: Ser46-Phosphorylated MARCKS Is a Marker of Neurite Degeneration at the Pre-aggregation Stage in PD/DLB Pathology
Article Snippet: .. The sections were washed twice for 5 min each with PBST (PBS containing 0.1% Tween 20), treated with PBS containing 0.5% Triton X-100 for 20 min, and washed three times with PBST for 5 min. After blocking (10% FBS for 30 min at 37°C), the sections were incubated sequentially with PBS containing 2% FBS and 0.1% Triton X-100, primary antibodies [mouse anti–phospho-α-Syn (Ser129; 1:2000, Wako, 015-25191) for 60 min at 37°C; mouse anti–α-Syn (1:1000, Abcam, ab27766) for 12 h at 4°C; rabbit anti–phospho-MARCKS (Ser46; 1:1000, GL Biochem) for 120 min at 37°C; or mouse anti-ubiquitin antibody (1:1000, Cell Signaling Technology, 3936S); rabbit anti–phospho-ERK1/2 detecting mouse Thr203/Tyr205-ERK1/Thr183/Tyr185-ERK2 and human Thr202/Tyr204-ERK1/Thr185/Tyr187-ERK2 (1:250, Cell Signaling Technology, 4370), mouse anti-MAP2 (1:100, Santa Cruz, sc-32791), or mouse anti-GFAP (1:2000, Sigma, C9205) for 12 h at 4°C], and secondary antibodies [Alexa Fluor 488–labeled anti-mouse IgG (1:1000, Invitrogen) or Cy3-labeled anti-rabbit IgG (1:500, Jackson ImmunoResearch) for 60 min at RT]. pSer46-MARCKS antibody was generated, and the quality was examined, as reported previously ( ). .. For double-labeling of pSer129-α-Syn and ubiquitin, anti–pSer129-α-Syn antibody was labeled with the Zenon Alexa Fluor 488 mouse IgG1 labeling kit (Z-25002, Invitrogen).

Blocking Assay:

Article Title: Identification of candidates for interacting partners of the tail domain of DcNMCP1, a major component of the Daucus carota nuclear lamina-like structure
Article Snippet: .. The membrane was incubated at 30°C for 1 h in PBS containing 1% (w/v) Block Ace (DS Pharma Biomedical, UKB40) and 1% (w/v) bovine serum albumin, washed twice with PBST (PBS supplemented with 0.2% (v/v) Tween 20), incubated at 30°C for 3 h in PBST containing either GST or GST-T975–1053 as probe (probe concentrations are indicated in ), washed 3 times with PBST, incubated at 25°C for 3 h in PBST containing Anti-GST, Monoclonal Antibody, Peroxidase Conjugated (Wako, 011–21891), and washed 3 times with PBST. .. Signals were detected using the Chemi-Lumi One L chemiluminescent substrate (Nacalai Tesque, 07880), and the LumiVision Pro 400EX imager (AISIN).

Article Title: Ser46-Phosphorylated MARCKS Is a Marker of Neurite Degeneration at the Pre-aggregation Stage in PD/DLB Pathology
Article Snippet: .. The sections were washed twice for 5 min each with PBST (PBS containing 0.1% Tween 20), treated with PBS containing 0.5% Triton X-100 for 20 min, and washed three times with PBST for 5 min. After blocking (10% FBS for 30 min at 37°C), the sections were incubated sequentially with PBS containing 2% FBS and 0.1% Triton X-100, primary antibodies [mouse anti–phospho-α-Syn (Ser129; 1:2000, Wako, 015-25191) for 60 min at 37°C; mouse anti–α-Syn (1:1000, Abcam, ab27766) for 12 h at 4°C; rabbit anti–phospho-MARCKS (Ser46; 1:1000, GL Biochem) for 120 min at 37°C; or mouse anti-ubiquitin antibody (1:1000, Cell Signaling Technology, 3936S); rabbit anti–phospho-ERK1/2 detecting mouse Thr203/Tyr205-ERK1/Thr183/Tyr185-ERK2 and human Thr202/Tyr204-ERK1/Thr185/Tyr187-ERK2 (1:250, Cell Signaling Technology, 4370), mouse anti-MAP2 (1:100, Santa Cruz, sc-32791), or mouse anti-GFAP (1:2000, Sigma, C9205) for 12 h at 4°C], and secondary antibodies [Alexa Fluor 488–labeled anti-mouse IgG (1:1000, Invitrogen) or Cy3-labeled anti-rabbit IgG (1:500, Jackson ImmunoResearch) for 60 min at RT]. pSer46-MARCKS antibody was generated, and the quality was examined, as reported previously ( ). .. For double-labeling of pSer129-α-Syn and ubiquitin, anti–pSer129-α-Syn antibody was labeled with the Zenon Alexa Fluor 488 mouse IgG1 labeling kit (Z-25002, Invitrogen).

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    FUJIFILM pbst
    Pbst, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/FUJIFILM
    Average 92 stars, based on 81 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    FUJIFILM tween 20 pbst
    Tween 20 Pbst, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tween 20 pbst/product/FUJIFILM
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    tween 20 pbst - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    90
    FUJIFILM pbst buffer
    Pbst Buffer, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst buffer/product/FUJIFILM
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbst buffer - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

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