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Cell Signaling Technology Inc pbst
Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with <t>Alexa</t> 488 labeled secondary antibody for 1 h. After washing three times with <t>PBST,</t> the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P
Pbst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 686 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion"

Article Title: Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkv202

Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P
Figure Legend Snippet: Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P

Techniques Used: Plasmid Preparation, Expressing, Multiple Displacement Amplification, Flow Cytometry, Cytometry, Blocking Assay, Incubation, Labeling, Infection, Conjugation Assay

2) Product Images from "Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion"

Article Title: Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkv202

Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P
Figure Legend Snippet: Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P

Techniques Used: Plasmid Preparation, Expressing, Multiple Displacement Amplification, Flow Cytometry, Cytometry, Blocking Assay, Incubation, Labeling, Infection, Conjugation Assay

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    Cell Signaling Technology Inc pbst
    Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with <t>Alexa</t> 488 labeled secondary antibody for 1 h. After washing three times with <t>PBST,</t> the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P
    Pbst, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbst/product/Cell Signaling Technology Inc
    Average 92 stars, based on 687 article reviews
    Price from $9.99 to $1999.99
    pbst - by Bioz Stars, 2020-07
    92/100 stars
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    Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P

    Journal: Nucleic Acids Research

    Article Title: Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion

    doi: 10.1093/nar/gkv202

    Figure Lengend Snippet: Development of a NAEK-containing lentiviral vector as a vehicle for the selective delivery of nucleic acid macromolecules into cells with high expression of integrins. ( A ) Expression of integrin α v β 3 in MDA-MB-435S vs. MCF-7 cells as detected by flow cytometry. The trypsinized cells, after sequential blocking by 10% BSA and washing, were incubated with monoclonal integrin α v β 3 antibody (red line) and an isotype control IgG antibody (green line) overnight in parallel and then incubated with Alexa 488 labeled secondary antibody for 1 h. After washing three times with PBST, the treated cells were analyzed by Guava EasyCyte Plus. The level of integrin α v β 3 expression on the cell surface of MDA-MB-435S cells is almost 3-fold higher than that on MCF-7 cells according to flow cytometry. ( B ) Characterization of the enhanced infectivity of NAEK-containing lentiviral vectors (Vector) and vectors upon conjugation with RGD (Vector-RGD) or RAD (Vector-RAD) at different sites towards MDA-MB-435S cells. The significance was confirmed by one-way ANOVA test (* P

    Article Snippet: Afterward, the cells were washed with PBST three times, incubated with Alexa 488-labeled secondary antibody (1:1000) (Cell Signaling Technology) at RT for 1 h, washed with PBST three times and analyzed by Guava EasyCyte Plus (Guava Technologies).

    Techniques: Plasmid Preparation, Expressing, Multiple Displacement Amplification, Flow Cytometry, Cytometry, Blocking Assay, Incubation, Labeling, Infection, Conjugation Assay