Structured Review

AvesLabs pbst
Pbst, supplied by AvesLabs, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbst/product/AvesLabs
Average 92 stars, based on 6 article reviews
Price from $9.99 to $1999.99
pbst - by Bioz Stars, 2020-07
92/100 stars

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Related Articles

Staining:

Article Title: Cerebellar granule cells encode the expectation of reward
Article Snippet: .. To stain for GCaMP6f we used a polyclonal GFP chicken antibody at 1:2000 dilution in PBST (Aves Labs). .. We incubated both primary antibodies for 48 hours, followed by 3 hours in FITC donkey anti-chicken and Alexa-647 goat anti-mouse secondary antibodies (Jackson Immunoresearch), both at 1:500 dilution in PBST.

Incubation:

Article Title: Developmental Regulation of the Nuclear Ferritoid-Ferritin Complex of Avian Corneal Epithelial Cells: Roles of Systemic Factors and Thyroxine
Article Snippet: .. Membranes were then incubated overnight at 4°C in 1.25% dry milk in PBST containing primary antibodies at the following dilutions: anti-ferritin at 1:500 [6D11 (1mg/mL)], anti-ferritoid at 1:10000 (Aves Lab), or anti-α-tubulin at 1:3000 (Ana Spec). .. The membranes were washed 3 times in PBST for 5 minutes per wash and incubated with the secondary HRP conjugated antibody for 1 hour at RT in PBST at the following dilutions: goat-anti-mouse at 1:10000 (Sigma), extravidin at 1:10000 (Invitrogen), or sheep-anti-rabbit at 1:5000 (R & D Systems).

Article Title: Tuning arousal with optogenetic modulation of locus coeruleus neurons
Article Snippet: .. After a brief wash in PBST, sections were incubated in chicken anti-tyrosine hydroxylase (1:2000, Aves Labs, #TH) in block solution at 4 °C for approximately 16 h. Following 3 × 10 min washes in PBST, sections were incubated in biotinylated goat anti-chicken IgG secondary antibody (1:500, Vector Laboratories, #BA-9010) for 1 h at room temperature. .. Next, the sections were washed 3 × 10 min in PBST and incubated in an avidin-biotin ABC peroxidase solution (Vector Laboratories, #PK-6100) for 30 min at room temperature.

Article Title: Fast-spiking interneurons supply feed-forward control of bursting, calcium, and plasticity for efficient learning
Article Snippet: .. Slides were blocked for 1 hour in 10% Normal Donkey Serum (NDS) in 0.5% PBST then incubated overnight in primary antibody (1:500 chicken anti-YFP, Aves Labs Inc, GFP-1020; and 1:500 rabbit anti-parvalbumin, Swant Inc., PV 27), 3% NDS in 0.5% PBST. .. The following day, they were washed 3 times for 10 minutes each in 0.5% PBST and incubated for 1 hour in secondary antibody (1:1000 donkey anti-chicken 488, Jackson Immunoresearch 703-545-155; and 1:1000 donkey anti-rabbit 647, Invitrogen, A-31573), 3% NDS in 0.5% PBST and 1:2000 DAPI.

Blocking Assay:

Article Title: Tuning arousal with optogenetic modulation of locus coeruleus neurons
Article Snippet: .. After a brief wash in PBST, sections were incubated in chicken anti-tyrosine hydroxylase (1:2000, Aves Labs, #TH) in block solution at 4 °C for approximately 16 h. Following 3 × 10 min washes in PBST, sections were incubated in biotinylated goat anti-chicken IgG secondary antibody (1:500, Vector Laboratories, #BA-9010) for 1 h at room temperature. .. Next, the sections were washed 3 × 10 min in PBST and incubated in an avidin-biotin ABC peroxidase solution (Vector Laboratories, #PK-6100) for 30 min at room temperature.

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