pbs  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Name:
    Alexa Fluor 488 Polyclonal Antibody
    Description:
    Alexa Fluor 488 Polyclonal Antibody for ICC IHC Flow
    Catalog Number:
    a11094
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Antibodies and Secondary Detection|Cell Analysis
    Buy from Supplier


    Structured Review

    Thermo Fisher pbs
    Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor <t>AMD3100</t> at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either <t>PBS</t> or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.
    Alexa Fluor 488 Polyclonal Antibody for ICC IHC Flow
    https://www.bioz.com/result/pbs/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars

    Images

    1) Product Images from "CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph"

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095626

    Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.
    Figure Legend Snippet: Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.

    Techniques Used: Inhibition, Chemotaxis Assay, Mouse Assay, Labeling, Flow Cytometry, Cytometry

    2) Product Images from "CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph"

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0095626

    Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.
    Figure Legend Snippet: Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.

    Techniques Used: Inhibition, Chemotaxis Assay, Mouse Assay, Labeling, Flow Cytometry, Cytometry

    3) Product Images from "Rapid Immunomagnetic Negative Enrichment of Neutrophil Granulocytes from Murine Bone Marrow for Functional Studies In Vitro and In Vivo"

    Article Title: Rapid Immunomagnetic Negative Enrichment of Neutrophil Granulocytes from Murine Bone Marrow for Functional Studies In Vitro and In Vivo

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0017314

    Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.
    Figure Legend Snippet: Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.

    Techniques Used: Isolation, Flow Cytometry, Cytometry, Microscopy, Cell Tracking Assay, Software, Staining, Fluorescence

    Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.
    Figure Legend Snippet: Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.

    Techniques Used: Isolation, Flow Cytometry, Cytometry, Microscopy, Cell Tracking Assay, Software, Staining, Fluorescence

    4) Product Images from "NK Depletion Results in Increased CCL22 Secretion and Treg Levels in Lewis Lung Carcinoma via the Accumulation of CCL22-secreting CD11b+CD11c+ Cells"

    Article Title: NK Depletion Results in Increased CCL22 Secretion and Treg Levels in Lewis Lung Carcinoma via the Accumulation of CCL22-secreting CD11b+CD11c+ Cells

    Journal: International journal of cancer. Journal international du cancer

    doi: 10.1002/ijc.25281

    CD11b and CD11c are expressed on different cells in normal lung tissues. Frozen sections from animals injected with PBS along with rabbit serum (a) or anti-asialo GM1 (b) were prepared and immunoflourescently stained with Hoechst dye (nuclear staining, top left), rhodamine-conjugated anti-CD11b (lower left), and FITC-conjugated anti-CD11c (top right). A merge of the three images is shown (lower right). Insets are of higher magnification of the indicated fields.
    Figure Legend Snippet: CD11b and CD11c are expressed on different cells in normal lung tissues. Frozen sections from animals injected with PBS along with rabbit serum (a) or anti-asialo GM1 (b) were prepared and immunoflourescently stained with Hoechst dye (nuclear staining, top left), rhodamine-conjugated anti-CD11b (lower left), and FITC-conjugated anti-CD11c (top right). A merge of the three images is shown (lower right). Insets are of higher magnification of the indicated fields.

    Techniques Used: Injection, Staining

    CD11b + CD11c + cells secrete CCL22 in NK-depleted LLC-bearing lung tissues. (a) Lung dissociates from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 were immunoflourescently stained for CD11b, CD11c, Gr-1, F4/80, and NK1.1. Cells were first gated on CD11b + cells, and FACS was performed to isolate the CD11b + CD11c + , CD11b + NK1.1 + , CD11b + Gr-1 + , and CD11b + F4/80 + populations. The isolated fractions were then plated overnight at 5.0×10 5 cells/ml and ELISA was used to measure secreted CCL22 in the media. (b) CCL22 secretion of each fraction was multiplied by the population frequency (Freq.). Frequencies are reported as the percent of total lung dissociate.
    Figure Legend Snippet: CD11b + CD11c + cells secrete CCL22 in NK-depleted LLC-bearing lung tissues. (a) Lung dissociates from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 were immunoflourescently stained for CD11b, CD11c, Gr-1, F4/80, and NK1.1. Cells were first gated on CD11b + cells, and FACS was performed to isolate the CD11b + CD11c + , CD11b + NK1.1 + , CD11b + Gr-1 + , and CD11b + F4/80 + populations. The isolated fractions were then plated overnight at 5.0×10 5 cells/ml and ELISA was used to measure secreted CCL22 in the media. (b) CCL22 secretion of each fraction was multiplied by the population frequency (Freq.). Frequencies are reported as the percent of total lung dissociate.

    Techniques Used: Injection, Staining, FACS, Isolation, Enzyme-linked Immunosorbent Assay

    CD11b + CD11c + cells represent a novel component of the myeloid compartment in NK-depleted LLC-bearing lungs. Lung dissociates from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 were immunoflourescently stained for CD11b and CD11c and analyzed using flow cytometry.
    Figure Legend Snippet: CD11b + CD11c + cells represent a novel component of the myeloid compartment in NK-depleted LLC-bearing lungs. Lung dissociates from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 were immunoflourescently stained for CD11b and CD11c and analyzed using flow cytometry.

    Techniques Used: Injection, Staining, Flow Cytometry, Cytometry

    Splenocytes (a) or bone marrow (b) that was harvested from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 was immunofluorescently stained with CD11b and CD11c. The cells were then quantified using flow cytometry. No significant accumulation of CD11b + CD11c + cells was seen in either spleen or bone marrow.
    Figure Legend Snippet: Splenocytes (a) or bone marrow (b) that was harvested from animals injected with PBS or LLC along with rabbit serum or anti-asialo GM1 was immunofluorescently stained with CD11b and CD11c. The cells were then quantified using flow cytometry. No significant accumulation of CD11b + CD11c + cells was seen in either spleen or bone marrow.

    Techniques Used: Injection, Staining, Flow Cytometry, Cytometry

    Related Articles

    Staining:

    Article Title: Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor
    Article Snippet: .. Cells were then stained with rabbit anti-Alexa-Fluor488 antibody (A-11094, Thermo Fisher Scientific) and with rat anti-mSR-A6 ED-31 antibody (MBS215280, MyBioSource) for one hour at +4°C in a humidified chamber. .. Anti-mouse-MINUS (cross-reactive to rat antibodies) and anti-rabbit-PLUS PLA probes (conjugated with oligonucleotides) were added, and hybridization, ligation, amplification and detection steps were performed according to the manufacturer’s instructions (PLA Duolink, Olink) to generate an amplified fluorescent signal in areas where the antigens recognized by the two primary antibodies reside within less than 40 nm distance of each other.

    Immunostaining:

    Article Title: A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly
    Article Snippet: .. Immunostaining was performed by the Dako Autostainer Link 48 with the Envision Flex kit system (Dako) according to the manufacturer’s instructions using 1:250 diluted Alexa Fluor-488 antibody (Thermo Fisher, Cat. A-11094). .. More specifically, sections were incubated for 10 min with peroxidase-blocking reagent, 60 min with 1:250 diluted primary polyclonal rabbit anti-Alexa Fluor-488, 30 min with the EnVision FLEX/HRP Detection Reagent, 5 min with EnVision FLEX DAB+ Chromogen/EnVision FLEX Substrate Buffer mix, and 5 min with EnVision FLEX Hematoxylin.

    Immunolabeling:

    Article Title: Capturing the Cardiac Injury Response of Targeted Cell Populations via Cleared Heart Three-Dimensional Imaging
    Article Snippet: Our representative results from Actl6bCre ;Rosa26tdT mice show that the endogenous tdT protein signal seen in nerves of an uncleared P7 heart was faithfully recapitulated in nerves of both uncleared and cleared tdT immunolabeled P7 hearts ( ). .. To immunolabel tdT-positive nerves, a Red Fluorescent Protein (RFP) primary antibody (rabbit polyclonal; 1:200 dilution; Rockland #600–401-379) was used along with an Alexa Fluor 488 secondary antibody (goat polyclonal; 1:1000 dilution; Invitrogen #A-11008). .. To visualize the cleared heart by eye during immunostaining and imaging steps, an ultraviolet flashlight may be briefly used to illuminate the heart.

    Immunofluorescence:

    Article Title: Exploring intracellular space: function of the Min system in round-shaped Escherichia coli
    Article Snippet: .. Immunofluorescence microscopy using affinity-purified anti-FtsZ polyclonal antibody conjugated to Alexa 488 (Molecular Probes, Eugene, OR) was performed essentially as described previously ( ). .. Deconvolution of FtsZ immunofluorescence images was performed with a Deltavision system as described previously ( ).

    Article Title: Cullin-3 and its adaptor protein ANKFY1 determine the surface level of integrin β1 in endothelial cells
    Article Snippet: .. Antibodies The following antibodies were purchased from the manufacturers as indicated: goat anti-integrin β1 antibody (N-20, dilution 1:1000 for western blotting; Santa Cruz Biotechnology), mouse anti-integrin β1 antibody (P5D2, dilution 1:1000; R & D Systems), mouse anti-integrin β1 antibody (TS2/16, dilution 1:500 for western blotting and immunofluorescence; BioLegend, San Diego, USA), mouse anti-CUL3 antibody (CUL3-9, dilution 1:1000; Sigma-Aldrich), Alexa488-conjugated mouse anti-integrin β1 antibody (TS2/16, dilution 1:200; BioLegend), rabbit anti-integrin α2 antibody (EPR17338, dilution 1:6000 for western blotting, dilution 1:1000 for immunofluorescence; Abcam), mouse anti-ANKFY1 antibody (B-6, dilution 1:100 for western blotting, dilution 1:50 for immunofluorescence; Santa Cruz Biotechnology), mouse anti-paxillin antibody (349, dilution 1:200; BD Bioscience), mouse anti-vinculin antibody (hVIN-1, dilution 1:1000; Sigma-Aldrich), rabbit anti-EGFR antibody (D38B1, dilution 1:1000; Cell Signaling Technology), mouse anti-Calnexin antibody (ab2798, dilution 1:1000; Abcam), mouse anti-GAPDH antibody (5A12, dilution 1:6000; Wako, Tokyo, Japan), rabbit anti-HA antibody (Y-11, dilution 1:1000 for western blotting and immunofluorescence, dilution 1:100 for immunoprecipitation; Santa Cruz Biotechnology), mouse anti-Myc antibody (9E10, dilution 1:1000; Santa Cruz Biotechnology), mouse anti-FLAG antibody (M2, dilution 1:1000; Sigma-Aldrich), rabbit anti-Alexa488 antibody (A-11094, dilution 1:200; Thermo Fisher Scientific), HRP-conjugated anti-mouse IgG antibody (W4021, dilution 1:2000; Promega, Madison, USA), HRP-conjugated anti-rabbit IgG antibody (W4011, dilution 1:2000; Promega), HRP-conjugated anti-goat IgG antibody (V805A, dilution 1:2000; Promega), goat Alexa488-conjugated anti-mouse IgG antibody (A11001, dilution 1:2000; Molecular Probes, Eugene, USA), donkey Alexa488-conjugated anti-rat IgG antibody (A21208, dilution 1:2000; Molecular Probes), goat Alexa568-conjugated anti-mouse IgG antibody (A11004, dilution 1:2000; Molecular Probes), and goat Cy3-conjugated anti-rabbit IgG antibody (111-165-144, dilution 1:2000; Jackson ImmunoResearch Laboratories). .. Plasmids GFP-Rab5, GFP-Rab7, GFP-Rab11, and LAMP1-GFP were amplified with vectors kindly provided by Dr. Gregory D Fairn (St. Michael's Hospital, Toronto, Canada) using the following pairs of primers: 5′-ATGGTGAGCAAGGGCGAGGA-3′ (GFP sense primer), 5′-TTAGTTACTACAACACTGATT-3′ (Rab5 antisense primer), 5′-TCAGCAACTGCAGCTTTCTGC-3′ (Rab7 antisense primer), 5′-TTAGATGTTCTGACAGCACTG-3′ (Rab11 antisense primer), 5′-ATGGCGGCCCCCGGCAGCGCC-3′ (LAMP1 sense primer), and 5′-TTACTTGTACAGCTCGTCCATGCCG-3′ (GFP antisense primer).

    Microscopy:

    Article Title: Exploring intracellular space: function of the Min system in round-shaped Escherichia coli
    Article Snippet: .. Immunofluorescence microscopy using affinity-purified anti-FtsZ polyclonal antibody conjugated to Alexa 488 (Molecular Probes, Eugene, OR) was performed essentially as described previously ( ). .. Deconvolution of FtsZ immunofluorescence images was performed with a Deltavision system as described previously ( ).

    Affinity Purification:

    Article Title: Exploring intracellular space: function of the Min system in round-shaped Escherichia coli
    Article Snippet: .. Immunofluorescence microscopy using affinity-purified anti-FtsZ polyclonal antibody conjugated to Alexa 488 (Molecular Probes, Eugene, OR) was performed essentially as described previously ( ). .. Deconvolution of FtsZ immunofluorescence images was performed with a Deltavision system as described previously ( ).

    FACS:

    Article Title: Sequences in the cytoplasmic tail of SARS-CoV-2 spike facilitate syncytia formation
    Article Snippet: Approximately 106 cells were resuspended in complete medium containing an anti-HA AF488 conjugate (1:1000) and incubated at 37°C for 40 minutes. .. The cells were washed twice with ice cold FACS buffer and incubated with an anti-AF488 antibody (1:67, A-11094, Thermo Fischer Scientific; to quench non-internalised anti-HA AF488 conjugate), an anti-mouse AF647 antibody (1:300, Thermo Fischer Scientific, A31571; to relabel the non-internalised anti-HA conjugate) and an eFluor 780 fixable viability dye. .. Cells were washed three times in ice cold FACS buffer, fixed in 4% paraformaldehyde (PFA) for 20 minutes and washed a further two times in FACS buffer.

    Incubation:

    Article Title: Sequences in the cytoplasmic tail of SARS-CoV-2 spike facilitate syncytia formation
    Article Snippet: Approximately 106 cells were resuspended in complete medium containing an anti-HA AF488 conjugate (1:1000) and incubated at 37°C for 40 minutes. .. The cells were washed twice with ice cold FACS buffer and incubated with an anti-AF488 antibody (1:67, A-11094, Thermo Fischer Scientific; to quench non-internalised anti-HA AF488 conjugate), an anti-mouse AF647 antibody (1:300, Thermo Fischer Scientific, A31571; to relabel the non-internalised anti-HA conjugate) and an eFluor 780 fixable viability dye. .. Cells were washed three times in ice cold FACS buffer, fixed in 4% paraformaldehyde (PFA) for 20 minutes and washed a further two times in FACS buffer.

    other:

    Article Title: Differential phosphorylation signals control endocytosis of GPR15
    Article Snippet: Antibodies The following Abs were used: mouse anti-GPR15 (MAB3654) and anti-GRK5 (MAB4539) from R & D Systems; rabbit anti-GPR15 (NBP1-02651) from Novus Biologicals; mouse anti-Myc (05-724) from EMD Millipore; rabbit anti-Myc (sc-789), rabbit anti-GRK6 (sc-566), rabbit anti–β-tubulin (sc-9104), rabbit anti-GRK2 (sc-562), rabbit anti-GRK6 (sc-566), and rabbit anti–14-3-3β (sc-629) from Santa Cruz Biotechnology; rabbit anti–phospho-PKA substrate (p-PKA sub, 9624), rabbit anti–phospho-PKC substrate (p-PKC sub, 2261), rabbit anti-Rab7 (9367), rabbit anti-LAMP1 (9091), rabbit anti-EEA1 (3288), rabbit anti-PKA C-α (5842), and rabbit anti–β-arrestin1/2 (D24H9) from Cell Signaling Technology; sheep anti-TGN46 (AHP500GT) from AbD Serotec; AF488-mouse anti-HA (A-21287), rabbit anti-AF488 (A-11094), Cy3-goat anti-mouse immunoglobulin G (IgG; A10521), Cy3-goat anti-rabbit IgG (A10520), AF488–donkey anti-sheep IgG (A11015), AF647–goat anti-rabbit IgG (A21245), and RPE–goat anti-mouse IgG (M30004-1) from Life Technologies; horseradish peroxidase (HRP)–goat anti-rabbit IgG (PI-1000) and HRP–goat anti-mouse IgG (PI-2000) from Vector Laboratory; AF488-Fab fragment of goat anti-mouse IgG (115-547-003) and normal goat-anti mouse IgG (115-005-146) from Jackson ImmunoResearch Laboratories; AF488-streptavidin (405235) from BioLegend; normal goat serum from Thermo Fisher; and human AB serum from Sigma-Aldrich.

    Western Blot:

    Article Title: Cullin-3 and its adaptor protein ANKFY1 determine the surface level of integrin β1 in endothelial cells
    Article Snippet: .. Antibodies The following antibodies were purchased from the manufacturers as indicated: goat anti-integrin β1 antibody (N-20, dilution 1:1000 for western blotting; Santa Cruz Biotechnology), mouse anti-integrin β1 antibody (P5D2, dilution 1:1000; R & D Systems), mouse anti-integrin β1 antibody (TS2/16, dilution 1:500 for western blotting and immunofluorescence; BioLegend, San Diego, USA), mouse anti-CUL3 antibody (CUL3-9, dilution 1:1000; Sigma-Aldrich), Alexa488-conjugated mouse anti-integrin β1 antibody (TS2/16, dilution 1:200; BioLegend), rabbit anti-integrin α2 antibody (EPR17338, dilution 1:6000 for western blotting, dilution 1:1000 for immunofluorescence; Abcam), mouse anti-ANKFY1 antibody (B-6, dilution 1:100 for western blotting, dilution 1:50 for immunofluorescence; Santa Cruz Biotechnology), mouse anti-paxillin antibody (349, dilution 1:200; BD Bioscience), mouse anti-vinculin antibody (hVIN-1, dilution 1:1000; Sigma-Aldrich), rabbit anti-EGFR antibody (D38B1, dilution 1:1000; Cell Signaling Technology), mouse anti-Calnexin antibody (ab2798, dilution 1:1000; Abcam), mouse anti-GAPDH antibody (5A12, dilution 1:6000; Wako, Tokyo, Japan), rabbit anti-HA antibody (Y-11, dilution 1:1000 for western blotting and immunofluorescence, dilution 1:100 for immunoprecipitation; Santa Cruz Biotechnology), mouse anti-Myc antibody (9E10, dilution 1:1000; Santa Cruz Biotechnology), mouse anti-FLAG antibody (M2, dilution 1:1000; Sigma-Aldrich), rabbit anti-Alexa488 antibody (A-11094, dilution 1:200; Thermo Fisher Scientific), HRP-conjugated anti-mouse IgG antibody (W4021, dilution 1:2000; Promega, Madison, USA), HRP-conjugated anti-rabbit IgG antibody (W4011, dilution 1:2000; Promega), HRP-conjugated anti-goat IgG antibody (V805A, dilution 1:2000; Promega), goat Alexa488-conjugated anti-mouse IgG antibody (A11001, dilution 1:2000; Molecular Probes, Eugene, USA), donkey Alexa488-conjugated anti-rat IgG antibody (A21208, dilution 1:2000; Molecular Probes), goat Alexa568-conjugated anti-mouse IgG antibody (A11004, dilution 1:2000; Molecular Probes), and goat Cy3-conjugated anti-rabbit IgG antibody (111-165-144, dilution 1:2000; Jackson ImmunoResearch Laboratories). .. Plasmids GFP-Rab5, GFP-Rab7, GFP-Rab11, and LAMP1-GFP were amplified with vectors kindly provided by Dr. Gregory D Fairn (St. Michael's Hospital, Toronto, Canada) using the following pairs of primers: 5′-ATGGTGAGCAAGGGCGAGGA-3′ (GFP sense primer), 5′-TTAGTTACTACAACACTGATT-3′ (Rab5 antisense primer), 5′-TCAGCAACTGCAGCTTTCTGC-3′ (Rab7 antisense primer), 5′-TTAGATGTTCTGACAGCACTG-3′ (Rab11 antisense primer), 5′-ATGGCGGCCCCCGGCAGCGCC-3′ (LAMP1 sense primer), and 5′-TTACTTGTACAGCTCGTCCATGCCG-3′ (GFP antisense primer).

    Immunoprecipitation:

    Article Title: Cullin-3 and its adaptor protein ANKFY1 determine the surface level of integrin β1 in endothelial cells
    Article Snippet: .. Antibodies The following antibodies were purchased from the manufacturers as indicated: goat anti-integrin β1 antibody (N-20, dilution 1:1000 for western blotting; Santa Cruz Biotechnology), mouse anti-integrin β1 antibody (P5D2, dilution 1:1000; R & D Systems), mouse anti-integrin β1 antibody (TS2/16, dilution 1:500 for western blotting and immunofluorescence; BioLegend, San Diego, USA), mouse anti-CUL3 antibody (CUL3-9, dilution 1:1000; Sigma-Aldrich), Alexa488-conjugated mouse anti-integrin β1 antibody (TS2/16, dilution 1:200; BioLegend), rabbit anti-integrin α2 antibody (EPR17338, dilution 1:6000 for western blotting, dilution 1:1000 for immunofluorescence; Abcam), mouse anti-ANKFY1 antibody (B-6, dilution 1:100 for western blotting, dilution 1:50 for immunofluorescence; Santa Cruz Biotechnology), mouse anti-paxillin antibody (349, dilution 1:200; BD Bioscience), mouse anti-vinculin antibody (hVIN-1, dilution 1:1000; Sigma-Aldrich), rabbit anti-EGFR antibody (D38B1, dilution 1:1000; Cell Signaling Technology), mouse anti-Calnexin antibody (ab2798, dilution 1:1000; Abcam), mouse anti-GAPDH antibody (5A12, dilution 1:6000; Wako, Tokyo, Japan), rabbit anti-HA antibody (Y-11, dilution 1:1000 for western blotting and immunofluorescence, dilution 1:100 for immunoprecipitation; Santa Cruz Biotechnology), mouse anti-Myc antibody (9E10, dilution 1:1000; Santa Cruz Biotechnology), mouse anti-FLAG antibody (M2, dilution 1:1000; Sigma-Aldrich), rabbit anti-Alexa488 antibody (A-11094, dilution 1:200; Thermo Fisher Scientific), HRP-conjugated anti-mouse IgG antibody (W4021, dilution 1:2000; Promega, Madison, USA), HRP-conjugated anti-rabbit IgG antibody (W4011, dilution 1:2000; Promega), HRP-conjugated anti-goat IgG antibody (V805A, dilution 1:2000; Promega), goat Alexa488-conjugated anti-mouse IgG antibody (A11001, dilution 1:2000; Molecular Probes, Eugene, USA), donkey Alexa488-conjugated anti-rat IgG antibody (A21208, dilution 1:2000; Molecular Probes), goat Alexa568-conjugated anti-mouse IgG antibody (A11004, dilution 1:2000; Molecular Probes), and goat Cy3-conjugated anti-rabbit IgG antibody (111-165-144, dilution 1:2000; Jackson ImmunoResearch Laboratories). .. Plasmids GFP-Rab5, GFP-Rab7, GFP-Rab11, and LAMP1-GFP were amplified with vectors kindly provided by Dr. Gregory D Fairn (St. Michael's Hospital, Toronto, Canada) using the following pairs of primers: 5′-ATGGTGAGCAAGGGCGAGGA-3′ (GFP sense primer), 5′-TTAGTTACTACAACACTGATT-3′ (Rab5 antisense primer), 5′-TCAGCAACTGCAGCTTTCTGC-3′ (Rab7 antisense primer), 5′-TTAGATGTTCTGACAGCACTG-3′ (Rab11 antisense primer), 5′-ATGGCGGCCCCCGGCAGCGCC-3′ (LAMP1 sense primer), and 5′-TTACTTGTACAGCTCGTCCATGCCG-3′ (GFP antisense primer).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Thermo Fisher pbs
    Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor <t>AMD3100</t> at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either <t>PBS</t> or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.
    Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    95
    Thermo Fisher syto rnaselect green fluorescent dye in pbs
    Translation events in Tau-positive neurites are a result of local protein synthesis (A) Cells grown for 9 DIV and treated with DMSO for 24 h. Cells immunostained with an anti-Tau antibody (magenta) were incubated with <t>SYTO</t> <t>RNASelect</t> green fluorescent dye to label endogenous RNA (green). Total green fluorescence intensity was measured in neurites covering a distance of 150 μm from the edge of the soma (2, + SYTO). As negative control, green fluorescence was measured in cells that had not been incubated with SYTO (1, -SYTO). To determine if SYTO selectively labeled RNA, some fixed cells were digested with DNAse (3, +SYTO +DNAse) or with RNAse (4, +SYTO +RNAse). Box and whisker graphs represent the average relative fluorescence intensity of 10 neurites per condition, shown as individual data points, and the mean and median of 5 (n=5, -SYTO negative samples compared to their corresponding +SYTO controls) or 6 (n=6, +SYTO + DNAse and +SYTO +RNAse compared to their corresponding + SYTO controls) independent experiments. *** p
    Syto Rnaselect Green Fluorescent Dye In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/syto rnaselect green fluorescent dye in pbs/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    syto rnaselect green fluorescent dye in pbs - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    N/A
    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
      Buy from Supplier

    Image Search Results


    Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.

    Journal: PLoS ONE

    Article Title: CXCR4 Is Dispensable for T Cell Egress from Chronically Inflamed Skin via the Afferent Lymph

    doi: 10.1371/journal.pone.0095626

    Figure Lengend Snippet: Pharmacological inhibition of CXCR4 does not impact the tissue exit of wildtype or Ccr7 −/− T cells. ( A–B ) Chemotaxis of mouse splenocytes was tested toward mouse CXCL12 ( A ) and CCL21 ( B ) in a Transwell chemotaxis assay in the presence or absence of CXCR4 inhibitor AMD3100 at indicated concentrations. Data represent mean ±SD of triplicate wells. One of two similar experiments is shown. ( C–E ) Mice carrying 3-week-old skin granulomas in the footpads were systemically treated with either PBS or 1000 µg/kg/h AMD3100 through subcutaneously implanted mini osmotic pumps. 12 h after implantation, a mixture of fluorescently labeled Ccr7 −/− and wildtype splenocytes were transferred into the inflamed footpads. The numbers and phenotypes of cells that egressed from the skin and entered the draining lymph node were determined by flow cytometry 12 h after transfer. The numbers of wildtype ( C ) and Ccr7 −/− ( D ) lymphocyte subsets that migrated to the draining lymph nodes are shown. Data points represent individually analyzed mice of groups of 7–10 recipient mice per group; horizontal lines indicate the mean of each group. One of two experiments with similar results ( C and D ) or both experiments combined ( E ) are shown. WT, wildtype.

    Article Snippet: Sterile-filtered AMD3100 in PBS (Sigma-Aldrich) or PBS alone (Invitrogen) was loaded into the osmotic pumps before equilibration in sterile PBS at 37°C for 12 h according to the manufacturer’s instructions.

    Techniques: Inhibition, Chemotaxis Assay, Mouse Assay, Labeling, Flow Cytometry, Cytometry

    Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.

    Journal: PLoS ONE

    Article Title: Rapid Immunomagnetic Negative Enrichment of Neutrophil Granulocytes from Murine Bone Marrow for Functional Studies In Vitro and In Vivo

    doi: 10.1371/journal.pone.0017314

    Figure Lengend Snippet: Neutrophil characterization. (a) After neutrophil preparation following the positive or negative isolation protocol the cells were treated with either PBS, PMA, fMLP or LPS for 15 minutes and the change of positive cells for CD62L in % on total living cells was estimated relative to freshly isolated, untreated cells by flow cytometry. Values are means of three independent experiments. (b) Positively (+) or negatively (−) isolated neutrophils were observed by time-lapse video microscopy in the presence of either PBS, PMA, fMLP or LPS for 3 h and cell velocity was assessed using a cell tracking software module. A total of 120 cells in 3 independent experiments per condition were analyzed. Black bars indicate the mean velocity values. (c) Right after positive (black bars) or negative isolation (grey bars) neutrophils were treated with PBS, PMA, fMLP or LPS for 15 min, subsequently stained with the ROS indicator DCFH and analyzed by flow cytometry for the occurrence of green = ROS positive cells. The mean fluorescence intensity (MFI) values for the measurements are stated above the particular bars. All values are means of four independent experiments except for the positively isolated and PBS treated population for which three independent experiments were analyzed.

    Article Snippet: Determination of ROS production Following either positive or negative isolation of neutrophils 1*106 cells were resuspended in 1 ml CM and stimulated with either PBS [10 µl/ml], PMA [10 ng/ml] , fMLP [0.1 mM] or LPS [2.5 ng/ml] at 37°C for 15 min. Then the cells were immediately cooled down to 4°C, washed once with 1 ml PBS and resuspended in 500 µl ROS detection solution (CM-H2DCFDA (Invitrogen) [1 µM in PBS]) supplemented with an anti-Gr-1-APC antibody to simultaneously stain the neutrophil population.

    Techniques: Isolation, Flow Cytometry, Cytometry, Microscopy, Cell Tracking Assay, Software, Staining, Fluorescence

    Translation events in Tau-positive neurites are a result of local protein synthesis (A) Cells grown for 9 DIV and treated with DMSO for 24 h. Cells immunostained with an anti-Tau antibody (magenta) were incubated with SYTO RNASelect green fluorescent dye to label endogenous RNA (green). Total green fluorescence intensity was measured in neurites covering a distance of 150 μm from the edge of the soma (2, + SYTO). As negative control, green fluorescence was measured in cells that had not been incubated with SYTO (1, -SYTO). To determine if SYTO selectively labeled RNA, some fixed cells were digested with DNAse (3, +SYTO +DNAse) or with RNAse (4, +SYTO +RNAse). Box and whisker graphs represent the average relative fluorescence intensity of 10 neurites per condition, shown as individual data points, and the mean and median of 5 (n=5, -SYTO negative samples compared to their corresponding +SYTO controls) or 6 (n=6, +SYTO + DNAse and +SYTO +RNAse compared to their corresponding + SYTO controls) independent experiments. *** p

    Journal: bioRxiv

    Article Title: Object-based analyses in FIJI/ImageJ to measure local RNA translation sites in neurites in response to Aβ1-42 oligomers

    doi: 10.1101/2020.01.27.921494

    Figure Lengend Snippet: Translation events in Tau-positive neurites are a result of local protein synthesis (A) Cells grown for 9 DIV and treated with DMSO for 24 h. Cells immunostained with an anti-Tau antibody (magenta) were incubated with SYTO RNASelect green fluorescent dye to label endogenous RNA (green). Total green fluorescence intensity was measured in neurites covering a distance of 150 μm from the edge of the soma (2, + SYTO). As negative control, green fluorescence was measured in cells that had not been incubated with SYTO (1, -SYTO). To determine if SYTO selectively labeled RNA, some fixed cells were digested with DNAse (3, +SYTO +DNAse) or with RNAse (4, +SYTO +RNAse). Box and whisker graphs represent the average relative fluorescence intensity of 10 neurites per condition, shown as individual data points, and the mean and median of 5 (n=5, -SYTO negative samples compared to their corresponding +SYTO controls) or 6 (n=6, +SYTO + DNAse and +SYTO +RNAse compared to their corresponding + SYTO controls) independent experiments. *** p

    Article Snippet: Following the standard immunocytochemistry procedure, cells were incubated for 20 min at room temperature with SYTO RNASelect green fluorescent dye in PBS (1:10.000, S-32703, Invitrogen).

    Techniques: Incubation, Fluorescence, Negative Control, Labeling, Whisker Assay