pbs  (Millipore)

 
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  • 86
    Name:
    Dulbecco s Phosphate Buffered Saline
    Description:

    Catalog Number:
    d8537
    Price:
    None
    Applications:
    DPBS is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.
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    Structured Review

    Millipore pbs

    https://www.bioz.com/result/pbs/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Purification:

    Article Title: Gelatin Type A from Porcine Skin Used as Co-Initiator in a Radical Photo-Initiating System
    Article Snippet: .. Materials Gelatin (type A, Vetec reagent grade powder, gel strength = 300 g Bloom, Mn = 50,000–100,000) from porcine skin, poly(ethylene glycol) diacrylate (PEGDA, Mn = 700 g mol−1 ), camphorquinone (CQ, 97%) and Dulbecco′s phosphate buffered saline (DPBS, pH 7.0–7.3) were purchased from Sigma-Aldrich (Milano, Italy) and used as received without further purification. .. Deionized water (DIH2 O) was obtained from a reverse osmosis (RO) purification system.

    other:

    Article Title: Long-Term Modeling of SARS-CoV-2 Infection of In Vitro Cultured Polarized Human Airway Epithelium
    Article Snippet: Transepithelial electrical resistance (TEER).One hundred microliters of D-PBS was added to the apical chamber to determine the TEER using a Millicell ERS-2 volt-ohm meter (MilliporeSigma, Burlington, MA) following a previously used method ( ).

    Incubation:

    Article Title: Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes
    Article Snippet: After washing thrice with D-PBS, the oocytes were incubated with goat anti-MAD2 antibody (1:50 dilution, Santa Cruz Biotechnology, Dallas, TX, USA) as a primary antibody over night at 4°C and then washed three times with D-PBS. .. This was followed by incubation in Alexa Fluor® 594-conjugated anti-goat IgG secondary antibody (1:500 dilution, Invitrogen) for 60 min. After washing thrice with D-PBS, the oocytes were incubated with mouse anti-α-Tubulin monoclonal antibody (1:500 dilution, Sigma) as a primary antibody for 60 min at room temperature, washed thrice with D-PBS, then incubated in fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG (1:100 dilution, Sigma) as a secondary antibody for 1 h at room temperature. .. Oocyte chromosomes were stained with 0.1 μg/ml 4',6-diamidino-2-phenylindole (DAPI, Sigma) for 30 min at 4°C.

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

    Article Title: Norwalk Virus-Like Particle Hemagglutination by Binding to H Histo-Blood Group Antigens
    Article Snippet: .. Neuraminidase from Arthrobacter ureafaciens (6 μU; Sigma) in Dulbecco's PBS (D-PBS) (Invitrogen) was combined with the RBCs and incubated for 60 min at 37°C. l -(Toslylamido-2-phenyl)ethyl chloromethyl ketone-treated trypsin (1.25 ng; Sigma) in D-PBS was added to the RBCs and incubated for 30 min at 37°C. .. Trypsin activity was stopped by adding 0.5 μg of soybean trypsin inhibitor (Sigma) to the reaction mixture.

    Exclusion Assay:

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

    Cell Culture:

    Article Title: Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci
    Article Snippet: VREfm Adherence to Caco-2 Cells In Vitro The adherence of VREfm to Caco-2 cells was inhibited using probiotics according to a previous procedure, with modifications [ ]. .. For the exclusion assay, Caco-2 cells in each well of 12-well plates forming confluent monolayers were washed twice with Dulbecco’s phosphate buffer saline (DPBS) (D5652, Sigma-Aldrich, St. Louis, MO, USA) and cultured with probiotic strains (approximately 3.33 × 108 CFU/mL) for 1 h. After incubation with probiotics, the Caco-2 cells were washed with DPBS twice and incubated with VREfm (approximately 3.33 × 106 CFU/mL) for another 1 h. Subsequently, the Caco-2 cells were washed twice with DPBS and then incubated with 0.05% Triton-X100 (T8787, Sigma-Aldrich, St. Louis, MO, USA) at 37 °C for 20 min. ..

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  • 97
    Millipore pbs solution
    a) Electrical benchtop recording output from the Ag <t>NWs</t> microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a <t>PBS</t> solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.
    Pbs Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    98
    Millipore sterile phosphate buffered saline pbs
    Comparison of mRNA expression induced by lipopolysaccharide <t>(LPS)</t> and lipoteichoic acid (LTA) in MLE-15 cells. MLE-15 cells were treated with 0–100 µ g/m l LPS (A) or LTA (B) at 37°C for 2 hr. MLE-15 cells treated with phosphate-buffered saline <t>(PBS)</t> served as a control. mRNA expression of SAA1/2 and SAA3 was normalized to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) mRNA expression and compared with that in control cells assumed to have 0 µ g/m l expression. Data are the mean plus standard deviation from three independent experiments. ** P
    Sterile Phosphate Buffered Saline Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile phosphate buffered saline pbs/product/Millipore
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    94
    Millipore sodium caseinate pbs
    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in <t>PBS</t> with the second component (30 pmol). Proteins were detected using the <t>Hbl</t> B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .
    Sodium Caseinate Pbs, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium caseinate pbs/product/Millipore
    Average 94 stars, based on 1 article reviews
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    sodium caseinate pbs - by Bioz Stars, 2021-03
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    a) Electrical benchtop recording output from the Ag NWs microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a PBS solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.

    Journal: bioRxiv

    Article Title: Flexible and transparent silver nanowire structures for multifunctional electrical and optical biointerfacing

    doi: 10.1101/2020.10.10.334755

    Figure Lengend Snippet: a) Electrical benchtop recording output from the Ag NWs microelectrodes, with 10 Hz, 20 mV peak-to-peak sine wave input. b) PSD of the signals recorded by the Ag NWs microelectrodes in (a). c) Soak test of the Ag NWs microelectrodes in a PBS solution at 37 °C. Z0 is the impedance at day 0, whereas Z represents the impedance at a specific day. d) CV curves of the Ag NWs microelectrodes at various scan rates.

    Article Snippet: CV tests were measured within a potential window from −0.8 to 0.8 V. The electrochemical measurements used a three-electrode configuration in a 1 × PBS solution (Sigma-Aldrich), where the Ag NWs microelectrodes, a platinum electrode, and an Ag/AgCl electrode served as the working, counter, and reference electrodes, respectively.

    Techniques:

    Comparison of mRNA expression induced by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) in MLE-15 cells. MLE-15 cells were treated with 0–100 µ g/m l LPS (A) or LTA (B) at 37°C for 2 hr. MLE-15 cells treated with phosphate-buffered saline (PBS) served as a control. mRNA expression of SAA1/2 and SAA3 was normalized to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) mRNA expression and compared with that in control cells assumed to have 0 µ g/m l expression. Data are the mean plus standard deviation from three independent experiments. ** P

    Journal: The Journal of Veterinary Medical Science

    Article Title: Lipopolysaccharide and lipoteichoic acid enhance serum amyloid A3 mRNA expression in murine alveolar epithelial cells

    doi: 10.1292/jvms.19-0154

    Figure Lengend Snippet: Comparison of mRNA expression induced by lipopolysaccharide (LPS) and lipoteichoic acid (LTA) in MLE-15 cells. MLE-15 cells were treated with 0–100 µ g/m l LPS (A) or LTA (B) at 37°C for 2 hr. MLE-15 cells treated with phosphate-buffered saline (PBS) served as a control. mRNA expression of SAA1/2 and SAA3 was normalized to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) mRNA expression and compared with that in control cells assumed to have 0 µ g/m l expression. Data are the mean plus standard deviation from three independent experiments. ** P

    Article Snippet: After incubation, the cells were rinsed with sterile phosphate-buffered saline (PBS) and treated with LPS from E. coli O111:B4 (115K4092, Sigma, St. Louis, MO, U.S.A.) or lipoteichoic acid (LTA) from Bacillus subtilis (L3265-5MG, Sigma), which are gram-negative and -positive bacterial membranous antigens, respectively.

    Techniques: Expressing, Standard Deviation

    pIC-exacerbated lung inflammation depends on IL-17RE. OVA-sensitized and challenged WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control. a Schema of the experimental protocol to study pIC-exacerbated OVA-induced allergic lung inflammation. Mice were treated with pIC or PBS as control 2 h after the final OVA challenge. BAL fluid concentrations of KC ( b ) and lung tissue concentrations of KC ( c ), MIP-2 ( d ), G-CSF ( e ), CCL5 ( f ), IL-5 ( g ), IL-13 ( h ), TNF-α ( i ), and IL-6 ( j ) were measured 22 h after treatment with pIC or PBS. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Journal: Respiratory Research

    Article Title: The IL-17 receptor IL-17RE mediates polyIC-induced exacerbation of experimental allergic asthma

    doi: 10.1186/s12931-020-01434-9

    Figure Lengend Snippet: pIC-exacerbated lung inflammation depends on IL-17RE. OVA-sensitized and challenged WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control. a Schema of the experimental protocol to study pIC-exacerbated OVA-induced allergic lung inflammation. Mice were treated with pIC or PBS as control 2 h after the final OVA challenge. BAL fluid concentrations of KC ( b ) and lung tissue concentrations of KC ( c ), MIP-2 ( d ), G-CSF ( e ), CCL5 ( f ), IL-5 ( g ), IL-13 ( h ), TNF-α ( i ), and IL-6 ( j ) were measured 22 h after treatment with pIC or PBS. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Article Snippet: 100 μg pIC (Sigma-Aldrich, St. Louis, USA) dissolved in 20 μl sterile PBS or 20 μl PBS without pIC were administrated intranasally.

    Techniques: Mouse Assay

    IL-17RE deficiency affects numbers of inflammatory cells in lung tissue. Asthmatic WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 22 h. a Numbers of immune cells in BAL fluids. b Representative IHC of Ly6B ( b ) and CD4 ( c ) and quantification of Ly6B + and CD4 + cells. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Journal: Respiratory Research

    Article Title: The IL-17 receptor IL-17RE mediates polyIC-induced exacerbation of experimental allergic asthma

    doi: 10.1186/s12931-020-01434-9

    Figure Lengend Snippet: IL-17RE deficiency affects numbers of inflammatory cells in lung tissue. Asthmatic WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 22 h. a Numbers of immune cells in BAL fluids. b Representative IHC of Ly6B ( b ) and CD4 ( c ) and quantification of Ly6B + and CD4 + cells. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Article Snippet: 100 μg pIC (Sigma-Aldrich, St. Louis, USA) dissolved in 20 μl sterile PBS or 20 μl PBS without pIC were administrated intranasally.

    Techniques: Mouse Assay, Immunohistochemistry

    IL-17RE regulates the pIC-induced expression of cytokines in asthmatic mice. Asthmatic WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 4 h. The lung tissue mRNA expression of IL-17C ( a ), KC ( b ), MIP-2 ( c ), G-CSF ( d ), and IL-17RE ( e ) were measure by semi-quantitative RT-PCR. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Journal: Respiratory Research

    Article Title: The IL-17 receptor IL-17RE mediates polyIC-induced exacerbation of experimental allergic asthma

    doi: 10.1186/s12931-020-01434-9

    Figure Lengend Snippet: IL-17RE regulates the pIC-induced expression of cytokines in asthmatic mice. Asthmatic WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 4 h. The lung tissue mRNA expression of IL-17C ( a ), KC ( b ), MIP-2 ( c ), G-CSF ( d ), and IL-17RE ( e ) were measure by semi-quantitative RT-PCR. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. *p

    Article Snippet: 100 μg pIC (Sigma-Aldrich, St. Louis, USA) dissolved in 20 μl sterile PBS or 20 μl PBS without pIC were administrated intranasally.

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR

    IL-17RE does not mediate pIC-induced lung inflammation in the absence of experimental allergic asthma. WT and Il-17re −/− mice were intranasally challenged with 100 μg pIC or PBS as control and analyzed after 24 h. Numbers of total cells ( a ), macrophages ( b ), neutrophils ( c ), and lymphocytes ( d ) were determined in BAL fluids. Concentrations of KC ( e ) and G-CSF ( f ) were measured in lung tissue. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. * p

    Journal: Respiratory Research

    Article Title: The IL-17 receptor IL-17RE mediates polyIC-induced exacerbation of experimental allergic asthma

    doi: 10.1186/s12931-020-01434-9

    Figure Lengend Snippet: IL-17RE does not mediate pIC-induced lung inflammation in the absence of experimental allergic asthma. WT and Il-17re −/− mice were intranasally challenged with 100 μg pIC or PBS as control and analyzed after 24 h. Numbers of total cells ( a ), macrophages ( b ), neutrophils ( c ), and lymphocytes ( d ) were determined in BAL fluids. Concentrations of KC ( e ) and G-CSF ( f ) were measured in lung tissue. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. * p

    Article Snippet: 100 μg pIC (Sigma-Aldrich, St. Louis, USA) dissolved in 20 μl sterile PBS or 20 μl PBS without pIC were administrated intranasally.

    Techniques: Mouse Assay

    IL-17RE deficiency effects methacholine-induced AHR. OVA-sensitized and challenged WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 22 h. Non-sensitized (PBS) control mice were treated with PBS. Mice were provoked with increasing concentrations of methacholine and the airway responsiveness was measured. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. **p

    Journal: Respiratory Research

    Article Title: The IL-17 receptor IL-17RE mediates polyIC-induced exacerbation of experimental allergic asthma

    doi: 10.1186/s12931-020-01434-9

    Figure Lengend Snippet: IL-17RE deficiency effects methacholine-induced AHR. OVA-sensitized and challenged WT and Il-17re −/− mice were intranasally treated with 100 μg pIC or PBS as control 2 h after the final OVA challenge and analyzed after 22 h. Non-sensitized (PBS) control mice were treated with PBS. Mice were provoked with increasing concentrations of methacholine and the airway responsiveness was measured. Data were compared by Two-way ANOVA with Tukey’s post-test and are shown as the mean ± SEM. **p

    Article Snippet: 100 μg pIC (Sigma-Aldrich, St. Louis, USA) dissolved in 20 μl sterile PBS or 20 μl PBS without pIC were administrated intranasally.

    Techniques: Mouse Assay

    Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Journal: Toxins

    Article Title: Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution

    doi: 10.3390/toxins9090288

    Figure Lengend Snippet: Detection of rHbl complex formation. ( A ) Dot blot. PVDF membranes were coated with rising concentrations (3.75–480 pmol) of different rHbl components. After blocking, the membrane was incubated in PBS with the second component (30 pmol). Proteins were detected using the Hbl B-specific mAb 1B8 [ 29 ] and the Hbl L 2 -specific mAb 1H9 (this study). Inversion of the protein order showed similar results and negative controls confirmed the specificity of the reaction (see Figure S1 ). ( B ) Indirect EIA. The first rHbl component was applied as serial dilution to a microtiter plate. After washing, the second rHbl component was applied in constant concentration (60 pmol/mL). After blocking, Hbl B-specific mAb 1B8 [ 29 ] and Hbl L 2 -specific mAb 1H9 (this study) were applied, respectively, followed by rabbit-anti-mouse-HRP conjugate for detection. Details on the non-linear regression are shown in Table S2 .

    Article Snippet: After that, Hbl-specific mAbs were applied (1B8 against Hbl B [ ], 1H9 against Hbl L2 (this study); 1E9 [ ] and 1G8 (this study) against Hbl L1 ; 3 μg/mL in 3% sodium-caseinate-PBS with 0.025% Tween 20) and overlaid on the dots for 1 h. The membrane was again washed for 3 × 10 min in PBS containing 0.1% Tween 20 before anti-mouse-IgG-alkaline-phosphatase-conjugate (Sigma) was applied (1:10.000 in 3% sodium-caseinate-PBS with 0.025% Tween 20) for 1 h. Again, the membrane was washed for 3 × 10 min in PBS containing 0.1% Tween 20 and for 2 × 10 min in PBS before signals were detected using NBT/BCIP solution (Roche).

    Techniques: Dot Blot, Blocking Assay, Incubation, Enzyme-linked Immunosorbent Assay, Serial Dilution, Concentration Assay