Structured Review

Merck KGaA pbs
Pbs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs/product/Merck KGaA
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
pbs - by Bioz Stars, 2021-03
86/100 stars

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Related Articles

Activity Assay:

Article Title: CX3C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke
Article Snippet: Immunohistochemistry Brain sections (thickness 30 µm) from 4% paraformaldehyde-perfused animals were rinsed in phosphate buffered saline (PBS). .. Endogenous peroxidase activity was quenched by washing the sections in a solution of PBS with 3% H2 O2 and 10% methanol for 15 min. After subsequent rinsing in PBS, blocking was achieved by incubation in a solution containing 5% normal donkey serum (NDS) in 0.25% Triton X-100 in PBS (Tx/PBS) for 1 h. Thereafter, sections were incubated with a mouse anti neuronal nuclei (NeuN) antibody (diluted at 1:1000, Merck Millipore, Billerica, MA, USA) at 4 °C in 5% NDS in Tx/PBS overnight. .. The next day, sections were rinsed in 1% NDS in Tx/PBS followed by incubation with respective biotinylated secondary antibodies in 2% NDS in Tx/PBS at room temperature for 90 min.

Blocking Assay:

Article Title: CX3C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke
Article Snippet: Immunohistochemistry Brain sections (thickness 30 µm) from 4% paraformaldehyde-perfused animals were rinsed in phosphate buffered saline (PBS). .. Endogenous peroxidase activity was quenched by washing the sections in a solution of PBS with 3% H2 O2 and 10% methanol for 15 min. After subsequent rinsing in PBS, blocking was achieved by incubation in a solution containing 5% normal donkey serum (NDS) in 0.25% Triton X-100 in PBS (Tx/PBS) for 1 h. Thereafter, sections were incubated with a mouse anti neuronal nuclei (NeuN) antibody (diluted at 1:1000, Merck Millipore, Billerica, MA, USA) at 4 °C in 5% NDS in Tx/PBS overnight. .. The next day, sections were rinsed in 1% NDS in Tx/PBS followed by incubation with respective biotinylated secondary antibodies in 2% NDS in Tx/PBS at room temperature for 90 min.

Article Title: Suppression of mRNAs encoding CD63 family tetraspanins from the carcinogenic liver fluke Opisthorchis viverrini results in distinct tegument phenotypes
Article Snippet: .. Proteins were transferred to nitrocellulose membrane (Mini Trans-Blot Cell, Bio-Rad), with PBST (1x PBS + 0.01% Tween-20), blocking was achieved using 5% skim milk and probed with either rabbit anti-Ov -TSP-2 or rabbit anti-Ov -TSP-3 at a dilution 1:1,000 overnight at 4 °C followed by anti-rabbit IgG-HRP (Merck millipore, USA) dilution 1:1,000. .. To identify antibodies in infected humans and hamsters, recombinant Ov -TSP-2 and Ov -TSP-3 were transblotted, probed with parasite-infected human or hamster serum (1:500) followed by secondary antibodies conjugated to HRP (1:1,000).

Incubation:

Article Title: CX3C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke
Article Snippet: Immunohistochemistry Brain sections (thickness 30 µm) from 4% paraformaldehyde-perfused animals were rinsed in phosphate buffered saline (PBS). .. Endogenous peroxidase activity was quenched by washing the sections in a solution of PBS with 3% H2 O2 and 10% methanol for 15 min. After subsequent rinsing in PBS, blocking was achieved by incubation in a solution containing 5% normal donkey serum (NDS) in 0.25% Triton X-100 in PBS (Tx/PBS) for 1 h. Thereafter, sections were incubated with a mouse anti neuronal nuclei (NeuN) antibody (diluted at 1:1000, Merck Millipore, Billerica, MA, USA) at 4 °C in 5% NDS in Tx/PBS overnight. .. The next day, sections were rinsed in 1% NDS in Tx/PBS followed by incubation with respective biotinylated secondary antibodies in 2% NDS in Tx/PBS at room temperature for 90 min.

Article Title: Impaired Fear Extinction Recall in Serotonin Transporter Knockout Rats Is Transiently Alleviated during Adolescence
Article Snippet: .. The free-floating sections were washed three times in PBS and preincubated with 0.3% perhydrol (30% H2O2, Merck, Darmstadt, Germany) for 30 min. After washing three times in PBS, the sections were presoaked for 30 min in an incubation medium consisting of PBS with 0.1% bovine serum albumin and 0.5% Triton X-100. .. The sections were then incubated with goat anti-GAD65/67, 1:2000 (Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), overnight on a shaker, at room temperature, and consecutively incubated for 90 min at room temperature with biotinylated donkey-anti-goat (Jackson Immuno Research Laboratories, West Grove, PA, USA) diluted 1:1500 in incubation medium and for 90 min at room temperature with ABC-elite, diluted 1:800 in PB (Vector Laboratories, Burlingame, CA, USA).

other:

Article Title: Preparation of PBS/PLLA/HAP Composites by the Solution Casting Method: Mechanical Properties and Biocompatibility
Article Snippet: Chemicals and MaterialsPLLA (MW–80,000–100,000; Polysciences Inc., Taipei, Taiwan), PBS (BioPBS, FZ91PM, PTT MCC Biochem Co. LTD., Bangkok, Thailand), calcium nitrate tetrahydrate (CA 0231; reagent grade, Scharlau, Barcelona, Spain), di-ammonium hydrogen phosphate (extra pure NF, Scharlau, Barcelona, Spain), ammonia solution (32%; Emplura Merck, Darmstadt, Germany), and chloroform (99-99.4: GC; Sigma Aldrich, Steinheim, Germany) were employed in this study.

Immunofluorescence:

Article Title: Characterization and identification of human immortalized granulosa cells derived from ovarian follicular fluid
Article Snippet: Flow cytometry data were analyzed with CXP software (EXPO32 v1.2; Beckman Coulter, Inc.). .. Immunofluorescence staining FCs and BMSc at passages 10 and 60 were fixed with 4% cold paraformaldehyde (Sigma-Aldrich; Merck KGaA) in PBS for 15 min at 4°C and permeabilized with 0.25% Triton X-100 (Sigma-Aldrich; Merck KGaA) in PBS for 10 min. .. Fixed cells were blocked for 30 min at 37°C with PBS containing 1% bovine serum albumin (BSA; Sigma-Aldrich; Merck KGaA) and 10% goat serum (Sigma-Aldrich; Merck KGaA), and incubated with goat anti-human FSHR (cat. no. sc7798; 1:100;), rabbit anti-human luteinizing hormone receptor (LHR; cat. no. sc25828; 1:100;) or mouse anti-human cytochrome P450 aromatase (CYP19A; cat. no. sc374176; 1:100; all from Santa Cruz Biotechnology, Inc.) antibodies at 4°C overnight.

Article Title: Expansion and Characterization of Neonatal Cardiac Pericytes Provides a Novel Cellular Option for Tissue Engineering in Congenital Heart Disease
Article Snippet: After staining, fluorescence was analyzed using a FACS Canto II flow cytometer and FACS Diva software (both BD Biosciences, UK). .. Immunofluorescence Analysis Cells at P5 were fixed with freshly prepared 4% (w/v) Paraformaldehyde for 20 minutes at room temperature, washed with PBS, and probed with the following antibodies: NG2 (1:100, Merck Millipore, UK), platelet-derived growth factor receptor-β (PDGFR-β) (1:50, Santa Cruz, UK), vimentin (1:100, Abcam, UK), GATA-4 (1:50, Santa Cruz), OCT-4 (1:400, Abcam, UK), SOX-2 (1:100, Merck Millipore), and c-Kit (1:40, DAKO, UK). ..

Staining:

Article Title: Characterization and identification of human immortalized granulosa cells derived from ovarian follicular fluid
Article Snippet: Flow cytometry data were analyzed with CXP software (EXPO32 v1.2; Beckman Coulter, Inc.). .. Immunofluorescence staining FCs and BMSc at passages 10 and 60 were fixed with 4% cold paraformaldehyde (Sigma-Aldrich; Merck KGaA) in PBS for 15 min at 4°C and permeabilized with 0.25% Triton X-100 (Sigma-Aldrich; Merck KGaA) in PBS for 10 min. .. Fixed cells were blocked for 30 min at 37°C with PBS containing 1% bovine serum albumin (BSA; Sigma-Aldrich; Merck KGaA) and 10% goat serum (Sigma-Aldrich; Merck KGaA), and incubated with goat anti-human FSHR (cat. no. sc7798; 1:100;), rabbit anti-human luteinizing hormone receptor (LHR; cat. no. sc25828; 1:100;) or mouse anti-human cytochrome P450 aromatase (CYP19A; cat. no. sc374176; 1:100; all from Santa Cruz Biotechnology, Inc.) antibodies at 4°C overnight.

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  • 86
    Merck KGaA pbs pgc 1α
    Protein synthesis and myotube diameter in C2C12 myotubes infected with AdshERRα and <t>PGC-1</t> adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, <t>PGC-1α,</t> or PGC-1β for a further 48 h. (A) ERRα protein, normalized to GAPDH protein. n = 3–4 per group. (B) Protein synthesis, measured via [ 3 H]-tyrosine incorporation for 24 hours after infections. n = 6 per group, repeated in three experiments. (C) Average myotube diameter from 10 myotubes per visual field (10 visual fields for each group). (D) Representative images of GFP, PGC-1α, and PGC-1β infected myotubes, with AdSUPER or AdshERRα. ∗∗∗ P
    Pbs Pgc 1α, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs pgc 1α/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs pgc 1α - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Merck KGaA pbs
    <t>SPS-neutralization</t> in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in <t>PBS</t> or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p
    Pbs, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Merck KGaA
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Protein synthesis and myotube diameter in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. (A) ERRα protein, normalized to GAPDH protein. n = 3–4 per group. (B) Protein synthesis, measured via [ 3 H]-tyrosine incorporation for 24 hours after infections. n = 6 per group, repeated in three experiments. (C) Average myotube diameter from 10 myotubes per visual field (10 visual fields for each group). (D) Representative images of GFP, PGC-1α, and PGC-1β infected myotubes, with AdSUPER or AdshERRα. ∗∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Protein synthesis and myotube diameter in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. (A) ERRα protein, normalized to GAPDH protein. n = 3–4 per group. (B) Protein synthesis, measured via [ 3 H]-tyrosine incorporation for 24 hours after infections. n = 6 per group, repeated in three experiments. (C) Average myotube diameter from 10 myotubes per visual field (10 visual fields for each group). (D) Representative images of GFP, PGC-1α, and PGC-1β infected myotubes, with AdSUPER or AdshERRα. ∗∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Infection, Pyrolysis Gas Chromatography

    Western blot analysis of Akt and p70S6k proteins in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. Samples were harvested after 96 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A–C . n = 4 per group. ∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Western blot analysis of Akt and p70S6k proteins in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. Samples were harvested after 96 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A–C . n = 4 per group. ∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Western Blot, Infection, Pyrolysis Gas Chromatography, Expressing

    Protein synthesis and myotube diameter in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. (A) PGC-1α and (B) PGC-1β protein 72 h after infection with GFP, PGC-1α, and PGC-1β adenoviruses. Bands were normalized to GAPDH protein; n = 4 per group. ∗∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Protein synthesis and myotube diameter in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. (A) PGC-1α and (B) PGC-1β protein 72 h after infection with GFP, PGC-1α, and PGC-1β adenoviruses. Bands were normalized to GAPDH protein; n = 4 per group. ∗∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Pyrolysis Gas Chromatography, Infection

    Protein synthesis and myotube diameter in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. (A) ERRα protein, normalized to GAPDH protein. n = 3–4 per group. (B) Protein synthesis, measured via [ 3 H]-tyrosine incorporation for 24 hours after infections. n = 6 per group, repeated in three experiments. (C) Average myotube diameter from 10 myotubes per visual field (10 visual fields for each group). (D) Representative images of GFP, PGC-1α, and PGC-1β infected myotubes, with AdSUPER or AdshERRα. ∗∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Protein synthesis and myotube diameter in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. (A) ERRα protein, normalized to GAPDH protein. n = 3–4 per group. (B) Protein synthesis, measured via [ 3 H]-tyrosine incorporation for 24 hours after infections. n = 6 per group, repeated in three experiments. (C) Average myotube diameter from 10 myotubes per visual field (10 visual fields for each group). (D) Representative images of GFP, PGC-1α, and PGC-1β infected myotubes, with AdSUPER or AdshERRα. ∗∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Infection, Pyrolysis Gas Chromatography

    mRNA expression of genes identified from the microarray based on their GO terms involved with protein synthesis, translation, and growth. Myotubes were infected with GFP, PGC-1α, or PGC-1β adenoviruses for 48 h, and samples were extracted after 72 h. mRNA of biasedly selected genes that were (A) upregulated and (B) downregulated in the microarray. mRNA expression of the genes selected unbiasedly from the microarray that were most significantly (C) upregulated and (D) downregulated. Values were normalized to 36B4 mRNA expression. n = 3, repeated in three experiments. ∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: mRNA expression of genes identified from the microarray based on their GO terms involved with protein synthesis, translation, and growth. Myotubes were infected with GFP, PGC-1α, or PGC-1β adenoviruses for 48 h, and samples were extracted after 72 h. mRNA of biasedly selected genes that were (A) upregulated and (B) downregulated in the microarray. mRNA expression of the genes selected unbiasedly from the microarray that were most significantly (C) upregulated and (D) downregulated. Values were normalized to 36B4 mRNA expression. n = 3, repeated in three experiments. ∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Expressing, Microarray, Infection, Pyrolysis Gas Chromatography

    Western blot analysis of Akt and p70S6k proteins in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. Myotubes were infected with GFP, PGC-1α, or PGC-1β adenoviruses for 48 h, and samples were extracted after 72 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Samples were harvested after 72 h of infection. Bands were normalized to GAPDH protein. The same control images have been used for A , C , and B , D . n = 5 per group. ∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Western blot analysis of Akt and p70S6k proteins in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. Myotubes were infected with GFP, PGC-1α, or PGC-1β adenoviruses for 48 h, and samples were extracted after 72 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Samples were harvested after 72 h of infection. Bands were normalized to GAPDH protein. The same control images have been used for A , C , and B , D . n = 5 per group. ∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Western Blot, Pyrolysis Gas Chromatography, Infection, Expressing

    Western blot analysis of Akt and p70S6k proteins in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. Samples were harvested after 96 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A–C . n = 4 per group. ∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Western blot analysis of Akt and p70S6k proteins in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. Samples were harvested after 96 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A–C . n = 4 per group. ∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Western Blot, Infection, Pyrolysis Gas Chromatography, Expressing

    Protein synthesis and myotube diameter in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. (A) PGC-1α and (B) PGC-1β protein 72 h after infection with GFP, PGC-1α, and PGC-1β adenoviruses. Bands were normalized to GAPDH protein; n = 4 per group. ∗∗∗ P

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Protein synthesis and myotube diameter in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. (A) PGC-1α and (B) PGC-1β protein 72 h after infection with GFP, PGC-1α, and PGC-1β adenoviruses. Bands were normalized to GAPDH protein; n = 4 per group. ∗∗∗ P

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Pyrolysis Gas Chromatography, Infection

    Overview of the GSEA performed on genes commonly regulated by both PGC-1α and PGC-1β in C2C12 myotubes. Proportional representation of gene numbers significantly enriched in GO-related (A) biological processes, (B) cellular compartment, and (C) molecular functions with the number of genes indicated in brackets. The proportion of genes making up the specific sub-groups; CC GO term mitochondrial inner membrane, and MF GO terms translation elongation factor activity are also indicated.

    Journal: Frontiers in Physiology

    Article Title: PGC-1α and PGC-1β Increase Protein Synthesis via ERRα in C2C12 Myotubes

    doi: 10.3389/fphys.2018.01336

    Figure Lengend Snippet: Overview of the GSEA performed on genes commonly regulated by both PGC-1α and PGC-1β in C2C12 myotubes. Proportional representation of gene numbers significantly enriched in GO-related (A) biological processes, (B) cellular compartment, and (C) molecular functions with the number of genes indicated in brackets. The proportion of genes making up the specific sub-groups; CC GO term mitochondrial inner membrane, and MF GO terms translation elongation factor activity are also indicated.

    Article Snippet: The membranes were blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS for 1 h, and incubated overnight at 4°C with the following primary antibodies diluted in 5% BSA in PBS: PGC-1α (Merck-Millipore); PGC-1β (Novus Biologicals, Littleton, CO, United States); ERRα (Epitomics, Burlingame, CA, United States); glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Sigma-Aldrich); Phospho-Akt (ser473), Akt, Phospho-p70S6k (thr389), p70S6k, Phospho-4E-BP1 (Thr37/46), and 4E-BP1 (Cell Signaling Technology, Danvers, MA, United States).

    Techniques: Pyrolysis Gas Chromatography, Activity Assay

    SPS-neutralization in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in PBS or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: SPS-neutralization in efficacy tests of SAAP-148 against gram-positive and gram-negative bacteria. Ninety μL of 10 7 CFU/mL MRSA (LUH14616 and Mu50), E. faecalis (ATCC 29212) , P. aeruginosa (PAO1; ATCC BAA47), E. coli (ATCC 35218) and A. baumannii were exposed to 10 μL of 1% (wt/v) SAAP-148 in PBS or PBS for 30 min. Subsequently, samples were homogenized in 500 μL of PBS with or without 0.05% (wt/v) SPS. The means and SD of six independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Neutralization

    Effect of SPS on the antimicrobial activity of various antimicrobial agents. Mixtures of 10 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS and 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS in PBS were prepared. Ninety μL of LUH14616 with a final concentration of 10 5 CFU/mL were added to these mixtures to determine the antimicrobial activity after 30 min incubation at 37 °C and 5% CO 2 . The means and standard deviations (SD) of three independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: Effect of SPS on the antimicrobial activity of various antimicrobial agents. Mixtures of 10 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS and 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS in PBS were prepared. Ninety μL of LUH14616 with a final concentration of 10 5 CFU/mL were added to these mixtures to determine the antimicrobial activity after 30 min incubation at 37 °C and 5% CO 2 . The means and standard deviations (SD) of three independent experiments performed in duplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Activity Assay, Concentration Assay, Incubation

    SPS-neutralization of residual activity of various antimicrobial agents. Excision wound models were inoculated with 10 5 CFU/mL LUH14616 for 1 h and exposed to 20 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS for 1 h. Subsequently, the models were homogenized in 1 mL of PBS with or without 0.05% (wt/v) SPS. The means and SD of at least eight independent experiments performed in triplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Journal: BMC Infectious Diseases

    Article Title: SPS-neutralization in tissue samples for efficacy testing of antimicrobial peptides

    doi: 10.1186/s12879-019-4700-1

    Figure Lengend Snippet: SPS-neutralization of residual activity of various antimicrobial agents. Excision wound models were inoculated with 10 5 CFU/mL LUH14616 for 1 h and exposed to 20 μL of SAAP-148 (1% wt/v), pexiganan (1% wt/v), chlorhexidine (0.5% v/v in 70% alcohol) or PBS for 1 h. Subsequently, the models were homogenized in 1 mL of PBS with or without 0.05% (wt/v) SPS. The means and SD of at least eight independent experiments performed in triplicate are shown. Results are expressed as the number of surviving bacteria in log10 CFU/mL. * indicates significant difference as compared to the samples without SPS (* p

    Article Snippet: SPS-neutralization of antimicrobial activity Ten μL of PBS or one of the antimicrobial agents: 1% (wt/v) SAAP-148 in PBS, 1% (wt/v) pexiganan in PBS, 1% (wt/wt) SSD, 0.5% (v/v) chlorhexidine in 70% alcohol, 2% (wt/wt) Bactroban or 2% (wt/wt) Fucidin were added to polypropylene vials containing 400 μL of PBS, 0.05, 0.1, 0.5% or 1% (wt/v; final concentrations) SPS (Fig. ) in PBS (Merck, KGaA, Darmstadt, Germany).

    Techniques: Neutralization, Activity Assay

    Evaluation of antitumor activity of 5-mG 2a -f (from day 7) in HSC-2 ×enografts. (A) HSC-2 cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 7, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 14 and 21. Tumor volume was measured on days 7, 12, 15, 19, 22, and 27. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Journal: Oncology Reports

    Article Title: A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.3892/or.2020.7735

    Figure Lengend Snippet: Evaluation of antitumor activity of 5-mG 2a -f (from day 7) in HSC-2 ×enografts. (A) HSC-2 cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 7, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 14 and 21. Tumor volume was measured on days 7, 12, 15, 19, 22, and 27. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Article Snippet: After day 1 (protocol-1) or day 7 (protocol-2), 100 µg of 5-mG2a-f and control mouse IgG (Sigma-Aldrich Corp.; Merck KGaA) in 100 µl PBS were injected intraperitoneally (i.p.) into treated and control mice, respectively.

    Techniques: Activity Assay, Injection, Mouse Assay

    Evaluation of antitumor activity of 5-mG 2a -f (from day 1) in HSC-2 ×enografts. (A) HSC-2 cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 1, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 7 and 14. Tumor volume was measured on days 6, 12, 15, and 19. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Journal: Oncology Reports

    Article Title: A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.3892/or.2020.7735

    Figure Lengend Snippet: Evaluation of antitumor activity of 5-mG 2a -f (from day 1) in HSC-2 ×enografts. (A) HSC-2 cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 1, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 7 and 14. Tumor volume was measured on days 6, 12, 15, and 19. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Article Snippet: After day 1 (protocol-1) or day 7 (protocol-2), 100 µg of 5-mG2a-f and control mouse IgG (Sigma-Aldrich Corp.; Merck KGaA) in 100 µl PBS were injected intraperitoneally (i.p.) into treated and control mice, respectively.

    Techniques: Activity Assay, Injection, Mouse Assay

    Evaluation of antitumor activity of 5-mG 2a -f (from day 1) in SAS xenografts. (A) SAS cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 1, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 7 and 14. Tumor volume was measured on days 6, 12, 15, and 19. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Journal: Oncology Reports

    Article Title: A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.3892/or.2020.7735

    Figure Lengend Snippet: Evaluation of antitumor activity of 5-mG 2a -f (from day 1) in SAS xenografts. (A) SAS cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 1, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 7 and 14. Tumor volume was measured on days 6, 12, 15, and 19. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Article Snippet: After day 1 (protocol-1) or day 7 (protocol-2), 100 µg of 5-mG2a-f and control mouse IgG (Sigma-Aldrich Corp.; Merck KGaA) in 100 µl PBS were injected intraperitoneally (i.p.) into treated and control mice, respectively.

    Techniques: Activity Assay, Injection, Mouse Assay

    Evaluation of ADCC and CDC activities by 5-mG 2a -f. (A) ADCC activities by 5-mG 2a -f, control mouse IgG 2a , and control PBS in SAS and HSC-2 cells. (B) CDC activities by 5-mG 2a -f, control mouse IgG 2a , and control PBS in SAS and HSC-2 cells. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Journal: Oncology Reports

    Article Title: A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.3892/or.2020.7735

    Figure Lengend Snippet: Evaluation of ADCC and CDC activities by 5-mG 2a -f. (A) ADCC activities by 5-mG 2a -f, control mouse IgG 2a , and control PBS in SAS and HSC-2 cells. (B) CDC activities by 5-mG 2a -f, control mouse IgG 2a , and control PBS in SAS and HSC-2 cells. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Article Snippet: After day 1 (protocol-1) or day 7 (protocol-2), 100 µg of 5-mG2a-f and control mouse IgG (Sigma-Aldrich Corp.; Merck KGaA) in 100 µl PBS were injected intraperitoneally (i.p.) into treated and control mice, respectively.

    Techniques:

    Evaluation of antitumor activity of 5-mG 2a -f (from day 7) in SAS xenografts. (A) SAS cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 7, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 14 and 21. Tumor volume was measured on days 7, 12, 15, 19, 22, and 27. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Journal: Oncology Reports

    Article Title: A defucosylated anti-CD44 monoclonal antibody 5-mG2a-f exerts antitumor effects in mouse xenograft models of oral squamous cell carcinoma

    doi: 10.3892/or.2020.7735

    Figure Lengend Snippet: Evaluation of antitumor activity of 5-mG 2a -f (from day 7) in SAS xenografts. (A) SAS cells (5×10 6 cells) were injected subcutaneously into the left flank. After day 7, 100 µg of 5-mG 2a -f and control mouse IgG in 100 µl PBS were injected i.p. into treated and control mice, respectively. Additional antibodies were then injected on days 14 and 21. Tumor volume was measured on days 7, 12, 15, 19, 22, and 27. Values are mean ± SEM. Asterisk indicates statistical significance (**P

    Article Snippet: After day 1 (protocol-1) or day 7 (protocol-2), 100 µg of 5-mG2a-f and control mouse IgG (Sigma-Aldrich Corp.; Merck KGaA) in 100 µl PBS were injected intraperitoneally (i.p.) into treated and control mice, respectively.

    Techniques: Activity Assay, Injection, Mouse Assay