pbs (Lonza)


Name:
Phosphate Buffered Saline
Description:
PBS 10X 67mM PO4 without Calcium and Magnesium 1 L
Catalog Number:
be17-517q
Price:
None
Category:
Culture Media and Reagents
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PBS 10X 67mM PO4 without Calcium and Magnesium 1 L
https://www.bioz.com/result/pbs/product/Lonza
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection"
Article Title: Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection
Journal: Scientific Reports
doi: 10.1038/s41598-018-36601-y

Figure Legend Snippet: Intracellular production of C. trachomatis RBs in VK2/E6E7 cells. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Images were taken 24 hr post C. trachomatis infection. ( A ) Intracellular C. trachomatis RB foci (green fluorescence) were visualized using fluorescence microscopy. Experiments were conducted n = 3 and a group of representative images were shown. ( B ) Semi-quantitative measurements of intracellular C. trachomatis RB foci were accomplished using Image J software. Values represent the mean ± SD, n = 3. *p
Techniques Used: Incubation, Infection, Fluorescence, Microscopy, Software

Figure Legend Snippet: In vitro quantitation of C. trachomatis genomes in the supernatant of VK2/E6E7 cell culture. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Supernatant containing newly produced C. trachomatis genomes was collected 24 hr post C. trachomatis infection and quantified by qRT-PCR. Values represent the mean ± SD, n = 3. *p
Techniques Used: In Vitro, Quantitation Assay, Cell Culture, Incubation, Infection, Produced, Quantitative RT-PCR
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