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    Name:
    Phosphate Buffered Saline
    Description:
    PBS 10X 67mM PO4 without Calcium and Magnesium 1 L
    Catalog Number:
    be17-517q
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    Culture Media and Reagents
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    Structured Review

    Lonza pbs
    Intracellular production of C. <t>trachomatis</t> RBs in VK2/E6E7 cells. Cells were incubated with <t>PBS,</t> non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Images were taken 24 hr post C. trachomatis infection. ( A ) Intracellular C. trachomatis RB foci (green fluorescence) were visualized using fluorescence microscopy. Experiments were conducted n = 3 and a group of representative images were shown. ( B ) Semi-quantitative measurements of intracellular C. trachomatis RB foci were accomplished using Image J software. Values represent the mean ± SD, n = 3. *p
    PBS 10X 67mM PO4 without Calcium and Magnesium 1 L
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    Images

    1) Product Images from "Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection"

    Article Title: Autophagy induction and PDGFR-β knockdown by siRNA-encapsulated nanoparticles reduce chlamydia trachomatis infection

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-36601-y

    Intracellular production of C. trachomatis RBs in VK2/E6E7 cells. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Images were taken 24 hr post C. trachomatis infection. ( A ) Intracellular C. trachomatis RB foci (green fluorescence) were visualized using fluorescence microscopy. Experiments were conducted n = 3 and a group of representative images were shown. ( B ) Semi-quantitative measurements of intracellular C. trachomatis RB foci were accomplished using Image J software. Values represent the mean ± SD, n = 3. *p
    Figure Legend Snippet: Intracellular production of C. trachomatis RBs in VK2/E6E7 cells. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Images were taken 24 hr post C. trachomatis infection. ( A ) Intracellular C. trachomatis RB foci (green fluorescence) were visualized using fluorescence microscopy. Experiments were conducted n = 3 and a group of representative images were shown. ( B ) Semi-quantitative measurements of intracellular C. trachomatis RB foci were accomplished using Image J software. Values represent the mean ± SD, n = 3. *p

    Techniques Used: Incubation, Infection, Fluorescence, Microscopy, Software

    In vitro quantitation of C. trachomatis genomes in the supernatant of VK2/E6E7 cell culture. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Supernatant containing newly produced C. trachomatis genomes was collected 24 hr post C. trachomatis infection and quantified by qRT-PCR. Values represent the mean ± SD, n = 3. *p
    Figure Legend Snippet: In vitro quantitation of C. trachomatis genomes in the supernatant of VK2/E6E7 cell culture. Cells were incubated with PBS, non-silencing siRNA PLGA-PEG NP (1.334 mg/mL), non-silencing siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) or PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL), PDGFR-β siRNA-PEI-PLGA-PEG NP (1.334 mg/mL) and autophagy inhibitor (bafilomycin A, 50 mM), PDGFR-β siRNA PLGA-PEG NP (1.334 mg/mL) and autophagy inducer (rapamycin, 100 nM) for 48 hr and then infected with C. trachomatis. Supernatant containing newly produced C. trachomatis genomes was collected 24 hr post C. trachomatis infection and quantified by qRT-PCR. Values represent the mean ± SD, n = 3. *p

    Techniques Used: In Vitro, Quantitation Assay, Cell Culture, Incubation, Infection, Produced, Quantitative RT-PCR

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The adjuvant AlhydroGel elicits higher antibody titres than AddaVax when combined with HIV-1 subtype C gp140 from CAP256
    Article Snippet: To assess the gp140 antigenic structure, Ni-NTA HisSorb Plates (Qiagen, Hilden) were coated for two hours with 200 ng/well CAP256 SU GP140-FL-IP-His protein from three separate isolations ( n = 3) at room temperature. .. ELISA plates were washed with PBS (Lonza, Basel) and blocked using 5% non-fat milk (Sigma, St Louis) in PBS. .. Serial dilutions were made of anti-Env human monoclonal antibodies PG9, PG16, CAP256-VRC26_08, PGT128, PGT135, PGT145, VRC01, F105 and 447-52D in PBS + 5% non-fat milk, starting at 10 mg/ml and added to gp140 coated plates and incubated overnight at 4°C.

    Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
    Article Snippet: Preparation of ASC Conditioned Medium ASCs (1 × 106 cells/cm2 ) at passage 3 or passage 5 were seeded on a 100 mm dish (Falcon) and cultured in ADSC BulletKit™ Medium (Lonza). .. At 100% confluence, ASCs were washed with phosphate-buffered saline (PBS) and cultured with ADSC serum-free medium (Lonza) for 48 h. Medium was collected, filtered using a 0.22 mm syringe filter, and either immediately transferred to RPE cells or maintained in −80°C for further protein analysis using ELISA assay. .. In turn, ASCs were harvested for mRNA level detection using RT-PCR.

    Purification:

    Article Title: Rotavirus capsid VP6 tubular and spherical nanostructures act as local adjuvants when co‐delivered with norovirus VLPs
    Article Snippet: NoV GII.4‐1999 VLPs (GenBank reference strain, Accession number AF080551) and rVP6 antigens (Accession no. GQ477131) used for immunizations of animals were highly purified with multi‐step chromatographic procedures or various steps of ultrafiltration, as described previously , . .. The purified rVP6 was assembled into nanotubes in phosphate‐buffered saline (PBS) at pH 7·3–7·5 (Lonza, Verviers, Belgium) or nanospheres in a 50 mM sodium acetate buffer with 130 mM NaCl, pH 4·82 . .. The concentration of the proteins was determined using Pierce BCA protein assay (Thermo Scientific, Waltham, MA, USA).

    Cell Culture:

    Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress
    Article Snippet: Preparation of ASC Conditioned Medium ASCs (1 × 106 cells/cm2 ) at passage 3 or passage 5 were seeded on a 100 mm dish (Falcon) and cultured in ADSC BulletKit™ Medium (Lonza). .. At 100% confluence, ASCs were washed with phosphate-buffered saline (PBS) and cultured with ADSC serum-free medium (Lonza) for 48 h. Medium was collected, filtered using a 0.22 mm syringe filter, and either immediately transferred to RPE cells or maintained in −80°C for further protein analysis using ELISA assay. .. In turn, ASCs were harvested for mRNA level detection using RT-PCR.

    Concentration Assay:

    Article Title: (Sub)populations of extracellular vesicles released by TNF-α –triggered human endothelial cells promote vascular inflammation and monocyte migration
    Article Snippet: Cell culture and treatment HUVEC (BD Bioscience, cat # 354,151) at passage 3–5 were grown in EBM-2 (Lonza) supplemented with EGM-2 MV SingleQuot Kit (Lonza; except the SingleQuot Kit foetal bovine serum) and 5% exosome-depleted foetal bovine serum (EXO-FBS-250A-1, System Bioscience) up to 70–75% confluency to minimize apoptosis. .. Confluent cells were rinsed twice with PBS (Lonza) and TNF-α (ImmunoTools, cat: 11,343,015)–based inflammation induction in cells was then performed at a final concentration of 10 ng/mL in a refreshed medium for 24 h [ ]. .. Cells were incubated in a humidified atmosphere condition of 5% CO2 /95% O2 at 37°C and routinely checked to be free of mycoplasma contamination.

    Mouse Assay:

    Article Title: Crude Turmeric Extract Improves the Suppressive Effects of Lactobacillus rhamnosus GG on Allergic Inflammation in a Murine Model of House Dust Mite-Induced Asthma
    Article Snippet: .. Murine HDM-Induced Asthma Model BALB/c mice were intranasally sensitized on day 0 with 1 μg HDM (Greer Laboratories, Lenoir, USA)/40 μL PBS (Lonza, Walkersville, USA) or PBS alone under the mild anesthetic circumstance induced by isoflurane inhalation. .. The protocol was followed by intranasal challenges once a day from day 7 to 11 with 10 μg HDM/40 μL PBS or PBS alone (HDM-PBS = positive control groups or PBS-PBS = negative control groups) ( and ) ( , , , ).

    Labeling:

    Article Title: Extracellular vesicles from human liver stem cells inhibit renal cancer stem cell‐derived tumor growth in vitro and in vivo
    Article Snippet: For biodistribution experiments, MSC‐EVs and HLSC‐EVs were labeled during ultracentrifugation with 1 μM Vybrant Cell Tracers DiD fluorescent dye (TermoFisher Scientific, Waltham, MA) as previously described. .. Labeled EVs were therefore washed twice by ultracentrifugation in PBS (Lonza). .. Proliferation For proliferation assay, cells were plated at a concentration of 1,500 renal CSCs/well, in a 96‐multiwell plate and cultured with different doses of HLSC‐EVs or MSC‐EVs (5, 10 or 50 × 103 EV/target cell).

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  • pbs  (Lonza)
    98
    Lonza pbs
    CAP256 gp140-FL-IP-His protein α-Env <t>ELISA.</t> Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein <t>(PBS)</t> control.
    Pbs, supplied by Lonza, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 1 article reviews
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    95
    Lonza pbs buffer
    Spectral and binding characteristics of Hoechst-based probes. (a) Structures of the Hoechst derivatives analyzed in this work. (b) Absorption (dashed line) and emission (solid line) spectra of dyes used for the generation of the DNA probes. (c) Brightness of Hoechst 33342 and its derivatives after binding to specific DNA. Measurements performed in <t>PBS</t> containing 2 μM probe and 30 μM hairpin DNA <t>(hpDNA).</t> (d) Titration of 4 nM Hoechst 33342 and 10 nM Hoechst 5′-regioisomer conjugates with hpDNA. The data points are fitted to a single site binding equation. (e) Titration of 10 nM Hoechst 6′-regioisomer conjugates with hpDNA. 5-GeR-Hoechst and 6-SiR-Hoechst data points are fitted to a single site binding equation. In contrast, 6-TMR-Hoechst , 6-580CP-Hoechst and 6-610CP-Hoechst data points can be fitted to the two site-binding equation. All data points are presented as mean ± s.d, N ≥ 3. (f) Proposed model of rhodamine–Hoechst conjugate interaction with the target DNA. Minor groove binding results in a brighter complex compared to major groove binding. The docking results show 19 conformations of 610CP-Hoechst: all 5′-regioisomer conformers are positioned in the minor groove, 3 out of 19 6′-regioisomer conformers are positioned in the major groove. Docking models were built using the DNA structure taken from PDB ID: ; 8BNA .
    Pbs Buffer, supplied by Lonza, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CAP256 gp140-FL-IP-His protein α-Env ELISA. Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein (PBS) control.

    Journal: PLoS ONE

    Article Title: The adjuvant AlhydroGel elicits higher antibody titres than AddaVax when combined with HIV-1 subtype C gp140 from CAP256

    doi: 10.1371/journal.pone.0208310

    Figure Lengend Snippet: CAP256 gp140-FL-IP-His protein α-Env ELISA. Binding ELISAs using anti-Env human monoclonal antibodies to CAP256 gp140-FL-IP-His. (A) PGT128 and PGT135 binding indicate the presence of the Env V3-glycan supersite. Similarly, the CD4 binding site using VRC01 The V2-glycan epitope was detected with PG9 (A), but no binding was observed for the native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08 (B). In line with this, clear ELISA signals were observed for F105 and 446-52D, indicative of the presence of misfolded Env (A). (C) Control soluble, trimeric BG505_664-His Env protein performed as expected, with ELISA signals for native-like trimer-specific MAbs PG16, PGT145 and (CAP256) VRC26_08. (D) No binding of MAbs was observed for the no protein (PBS) control.

    Article Snippet: ELISA plates were washed with PBS (Lonza, Basel) and blocked using 5% non-fat milk (Sigma, St Louis) in PBS.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    House dust mite (HDM)-induced airway resistance diagram. The airway resistance was abrogated upon oral administration of 20 mg/kg TP (prebiotic), or 10 5 or 10 7 cfu/mouse LGG (probiotic) or TP in combination with 10 5 or 10 7 cfu/mouse of LGG (synbiotic). Lung resistance (RL) measured in response to increasing doses of methacholine. PBS-PBS (control negative group): PBS sensitized, challenged, and treated mice, PBS-TP: PBS sensitized and challenged, and TP (20 mg/kg) treated mice, PBS-TP- LGG E7: PBS sensitized and challenged, and synbiotic (with 10 7 cfu/mouse LGG) treated mice, HDM-PBS (control positive group): HDM sensitized and challenged, and PBS treated mice, HDM-CS: HDM sensitized and challenged, and corticosteroid treated mice, HDM- LGG E5: HDM sensitized and challenged, and 10 5 cfu/mouse probiotic treated mice, HDM-LGG E7: HDM sensitized and challenged, and 10 7 cfu/mouse probiotic treated mice. HDM-TP: HDM sensitized and challenged, and prebiotic treated mice, HDM-TP-LGG E5: HDM sensitized and challenged, and synbiotic (with 10 5 cfu/mouse LGG) treated mice, HDM-TP-LGG E7: HDM sensitized and challenged, and synbiotic (with 10 7 cfu/mouse LGG) treated mice. Results are shown as mean ± SEM . * P

    Journal: Frontiers in Immunology

    Article Title: Crude Turmeric Extract Improves the Suppressive Effects of Lactobacillus rhamnosus GG on Allergic Inflammation in a Murine Model of House Dust Mite-Induced Asthma

    doi: 10.3389/fimmu.2020.01092

    Figure Lengend Snippet: House dust mite (HDM)-induced airway resistance diagram. The airway resistance was abrogated upon oral administration of 20 mg/kg TP (prebiotic), or 10 5 or 10 7 cfu/mouse LGG (probiotic) or TP in combination with 10 5 or 10 7 cfu/mouse of LGG (synbiotic). Lung resistance (RL) measured in response to increasing doses of methacholine. PBS-PBS (control negative group): PBS sensitized, challenged, and treated mice, PBS-TP: PBS sensitized and challenged, and TP (20 mg/kg) treated mice, PBS-TP- LGG E7: PBS sensitized and challenged, and synbiotic (with 10 7 cfu/mouse LGG) treated mice, HDM-PBS (control positive group): HDM sensitized and challenged, and PBS treated mice, HDM-CS: HDM sensitized and challenged, and corticosteroid treated mice, HDM- LGG E5: HDM sensitized and challenged, and 10 5 cfu/mouse probiotic treated mice, HDM-LGG E7: HDM sensitized and challenged, and 10 7 cfu/mouse probiotic treated mice. HDM-TP: HDM sensitized and challenged, and prebiotic treated mice, HDM-TP-LGG E5: HDM sensitized and challenged, and synbiotic (with 10 5 cfu/mouse LGG) treated mice, HDM-TP-LGG E7: HDM sensitized and challenged, and synbiotic (with 10 7 cfu/mouse LGG) treated mice. Results are shown as mean ± SEM . * P

    Article Snippet: Murine HDM-Induced Asthma Model BALB/c mice were intranasally sensitized on day 0 with 1 μg HDM (Greer Laboratories, Lenoir, USA)/40 μL PBS (Lonza, Walkersville, USA) or PBS alone under the mild anesthetic circumstance induced by isoflurane inhalation.

    Techniques: Mouse Assay

    Schematic overview of the experimental protocol. BALB/c mice ( n = 6/group) were intranasally sensitized (i.n.) with house dust mite (HDM) or PBS on day 0 and were challenged i.n. for five consecutive days ( 7 – 11 ) with HDM or PBS. The oral gavage treatment started 2 weeks prior to sensitization. By oral gavage, the mice were received 20 mg/kg TP (prebiotic), or 10 5 or 10 7 cfu/mouse LGG (probiotic) or turmeric in combination with 10 5 or 10 7 cfu/mouse of LGG (synbiotic) once a day, throughout the study. The mice were sacrificed on day 14.

    Journal: Frontiers in Immunology

    Article Title: Crude Turmeric Extract Improves the Suppressive Effects of Lactobacillus rhamnosus GG on Allergic Inflammation in a Murine Model of House Dust Mite-Induced Asthma

    doi: 10.3389/fimmu.2020.01092

    Figure Lengend Snippet: Schematic overview of the experimental protocol. BALB/c mice ( n = 6/group) were intranasally sensitized (i.n.) with house dust mite (HDM) or PBS on day 0 and were challenged i.n. for five consecutive days ( 7 – 11 ) with HDM or PBS. The oral gavage treatment started 2 weeks prior to sensitization. By oral gavage, the mice were received 20 mg/kg TP (prebiotic), or 10 5 or 10 7 cfu/mouse LGG (probiotic) or turmeric in combination with 10 5 or 10 7 cfu/mouse of LGG (synbiotic) once a day, throughout the study. The mice were sacrificed on day 14.

    Article Snippet: Murine HDM-Induced Asthma Model BALB/c mice were intranasally sensitized on day 0 with 1 μg HDM (Greer Laboratories, Lenoir, USA)/40 μL PBS (Lonza, Walkersville, USA) or PBS alone under the mild anesthetic circumstance induced by isoflurane inhalation.

    Techniques: Mouse Assay

    Intravenous injection of HLSC‐EVs reduced tumor growth and delayed CSCs lung spread. ( a ) Tumor size (mm 3 ) of untreated mice (CTL), and of mice i.v. treated with MSC‐ or HLSC‐EVs during the experiment. ( b ) Tumor size (mm 3 ) of recovered plugs after sacrifice. ( c ) Evaluation of angiogenesis within plugs, expressed as number of vessels connected to mouse vasculature/field, and representative images of vessels stained with Masson's trichromic reaction. ( d ) Tumor apoptosis evaluation by Tunel assay, expressed as number of TUNEL positive cells/field, and representative micrographs showing apoptosis within tumors. ( a – d ) Data are expressed as mean ± SEM of n = 20 tumors for control group (CTL), n = 18 for HLSC‐EVs group and n = 14 for MSC‐EVs group. * p > 0.05 vs . CTL. ( e ) Representative micrographs showing IVIS analysis of lung CSCs foci after 10 days from i.v. injection of renal CSCs; ( f ) Kaplan–Meier curve showing the percentage of mice that did not develop lung tumors during the time of experiment. Data are expressed as mean ± SEM of 24 HLSC‐EVs and 24 PBS treated mice. * p = 0.0016. ( g ) Representative micrographs showing IVIS analysis of lungs at Week 3.

    Journal: International Journal of Cancer

    Article Title: Extracellular vesicles from human liver stem cells inhibit renal cancer stem cell‐derived tumor growth in vitro and in vivo

    doi: 10.1002/ijc.32925

    Figure Lengend Snippet: Intravenous injection of HLSC‐EVs reduced tumor growth and delayed CSCs lung spread. ( a ) Tumor size (mm 3 ) of untreated mice (CTL), and of mice i.v. treated with MSC‐ or HLSC‐EVs during the experiment. ( b ) Tumor size (mm 3 ) of recovered plugs after sacrifice. ( c ) Evaluation of angiogenesis within plugs, expressed as number of vessels connected to mouse vasculature/field, and representative images of vessels stained with Masson's trichromic reaction. ( d ) Tumor apoptosis evaluation by Tunel assay, expressed as number of TUNEL positive cells/field, and representative micrographs showing apoptosis within tumors. ( a – d ) Data are expressed as mean ± SEM of n = 20 tumors for control group (CTL), n = 18 for HLSC‐EVs group and n = 14 for MSC‐EVs group. * p > 0.05 vs . CTL. ( e ) Representative micrographs showing IVIS analysis of lung CSCs foci after 10 days from i.v. injection of renal CSCs; ( f ) Kaplan–Meier curve showing the percentage of mice that did not develop lung tumors during the time of experiment. Data are expressed as mean ± SEM of 24 HLSC‐EVs and 24 PBS treated mice. * p = 0.0016. ( g ) Representative micrographs showing IVIS analysis of lungs at Week 3.

    Article Snippet: Labeled EVs were therefore washed twice by ultracentrifugation in PBS (Lonza).

    Techniques: Injection, Mouse Assay, Staining, TUNEL Assay

    HLSC‐EVs induce antitumor miRNA expression both in vivo and in vitro . ( a ) Antitumor miRNAs expression on renal CSCs‐derived tumors, evaluated by real‐time PCR, after 4 weeks of EV‐treatment. Data are expressed as mean ± SD of Relative Quantification (RQ) normalized to PBS‐treated tumors (CTL) and to RNU6B of 10 CTL, 10 MSC‐ and 10 HLSC‐EV treated tumors. ** p

    Journal: International Journal of Cancer

    Article Title: Extracellular vesicles from human liver stem cells inhibit renal cancer stem cell‐derived tumor growth in vitro and in vivo

    doi: 10.1002/ijc.32925

    Figure Lengend Snippet: HLSC‐EVs induce antitumor miRNA expression both in vivo and in vitro . ( a ) Antitumor miRNAs expression on renal CSCs‐derived tumors, evaluated by real‐time PCR, after 4 weeks of EV‐treatment. Data are expressed as mean ± SD of Relative Quantification (RQ) normalized to PBS‐treated tumors (CTL) and to RNU6B of 10 CTL, 10 MSC‐ and 10 HLSC‐EV treated tumors. ** p

    Article Snippet: Labeled EVs were therefore washed twice by ultracentrifugation in PBS (Lonza).

    Techniques: Expressing, In Vivo, In Vitro, Derivative Assay, Real-time Polymerase Chain Reaction

    Spectral and binding characteristics of Hoechst-based probes. (a) Structures of the Hoechst derivatives analyzed in this work. (b) Absorption (dashed line) and emission (solid line) spectra of dyes used for the generation of the DNA probes. (c) Brightness of Hoechst 33342 and its derivatives after binding to specific DNA. Measurements performed in PBS containing 2 μM probe and 30 μM hairpin DNA (hpDNA). (d) Titration of 4 nM Hoechst 33342 and 10 nM Hoechst 5′-regioisomer conjugates with hpDNA. The data points are fitted to a single site binding equation. (e) Titration of 10 nM Hoechst 6′-regioisomer conjugates with hpDNA. 5-GeR-Hoechst and 6-SiR-Hoechst data points are fitted to a single site binding equation. In contrast, 6-TMR-Hoechst , 6-580CP-Hoechst and 6-610CP-Hoechst data points can be fitted to the two site-binding equation. All data points are presented as mean ± s.d, N ≥ 3. (f) Proposed model of rhodamine–Hoechst conjugate interaction with the target DNA. Minor groove binding results in a brighter complex compared to major groove binding. The docking results show 19 conformations of 610CP-Hoechst: all 5′-regioisomer conformers are positioned in the minor groove, 3 out of 19 6′-regioisomer conformers are positioned in the major groove. Docking models were built using the DNA structure taken from PDB ID: ; 8BNA .

    Journal: Chemical Science

    Article Title: Rhodamine–Hoechst positional isomers for highly efficient staining of heterochromatin positional isomers for highly efficient staining of heterochromatin †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc05082a

    doi: 10.1039/c8sc05082a

    Figure Lengend Snippet: Spectral and binding characteristics of Hoechst-based probes. (a) Structures of the Hoechst derivatives analyzed in this work. (b) Absorption (dashed line) and emission (solid line) spectra of dyes used for the generation of the DNA probes. (c) Brightness of Hoechst 33342 and its derivatives after binding to specific DNA. Measurements performed in PBS containing 2 μM probe and 30 μM hairpin DNA (hpDNA). (d) Titration of 4 nM Hoechst 33342 and 10 nM Hoechst 5′-regioisomer conjugates with hpDNA. The data points are fitted to a single site binding equation. (e) Titration of 10 nM Hoechst 6′-regioisomer conjugates with hpDNA. 5-GeR-Hoechst and 6-SiR-Hoechst data points are fitted to a single site binding equation. In contrast, 6-TMR-Hoechst , 6-580CP-Hoechst and 6-610CP-Hoechst data points can be fitted to the two site-binding equation. All data points are presented as mean ± s.d, N ≥ 3. (f) Proposed model of rhodamine–Hoechst conjugate interaction with the target DNA. Minor groove binding results in a brighter complex compared to major groove binding. The docking results show 19 conformations of 610CP-Hoechst: all 5′-regioisomer conformers are positioned in the minor groove, 3 out of 19 6′-regioisomer conformers are positioned in the major groove. Docking models were built using the DNA structure taken from PDB ID: ; 8BNA .

    Article Snippet: Estimation of absorbance and fluorescence increase upon SDS or hpDNA addition Fluorescence increase of the probes upon SDS or hpDNA addition was measured by preparing 2 μM probe solution in PBS buffer (Lonza, Cat. No. BE17-516F) with or without 0.1% SDS (Acros Organics).

    Techniques: Binding Assay, Titration