Structured Review

Fisher Scientific pbs
ELISA to show binding of rfhSP-D to (A) pH1N1 and (B) H3N2: <t>microtiter</t> wells were coated with different concentrations of rfhSP-D (5, 2.5, 1.25, and 0.625 µg/ml). 20 µl of concentrated pH1N1 or H3N2 virus (1.36 × 10 6 pfu/ml) was diluted in 200 µl of <t>PBS</t> + 5 mM CaCl 2 and 10 µl of diluted virus was added to all the wells, and probed with either monoclonal anti-influenza virus H1 or polyclonal anti-influenza virus H3 antibody. VSV-G pseudotyped lentivirus was used as a negative RNA virus control. The data were expressed as mean of three independent experiments done in triplicates ± SEM.
Pbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Entry Inhibition and Modulation of Pro-Inflammatory Immune Response Against Influenza A Virus by a Recombinant Truncated Surfactant Protein D"

Article Title: Entry Inhibition and Modulation of Pro-Inflammatory Immune Response Against Influenza A Virus by a Recombinant Truncated Surfactant Protein D

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2018.01586

ELISA to show binding of rfhSP-D to (A) pH1N1 and (B) H3N2: microtiter wells were coated with different concentrations of rfhSP-D (5, 2.5, 1.25, and 0.625 µg/ml). 20 µl of concentrated pH1N1 or H3N2 virus (1.36 × 10 6 pfu/ml) was diluted in 200 µl of PBS + 5 mM CaCl 2 and 10 µl of diluted virus was added to all the wells, and probed with either monoclonal anti-influenza virus H1 or polyclonal anti-influenza virus H3 antibody. VSV-G pseudotyped lentivirus was used as a negative RNA virus control. The data were expressed as mean of three independent experiments done in triplicates ± SEM.
Figure Legend Snippet: ELISA to show binding of rfhSP-D to (A) pH1N1 and (B) H3N2: microtiter wells were coated with different concentrations of rfhSP-D (5, 2.5, 1.25, and 0.625 µg/ml). 20 µl of concentrated pH1N1 or H3N2 virus (1.36 × 10 6 pfu/ml) was diluted in 200 µl of PBS + 5 mM CaCl 2 and 10 µl of diluted virus was added to all the wells, and probed with either monoclonal anti-influenza virus H1 or polyclonal anti-influenza virus H3 antibody. VSV-G pseudotyped lentivirus was used as a negative RNA virus control. The data were expressed as mean of three independent experiments done in triplicates ± SEM.

Techniques Used: Enzyme-linked Immunosorbent Assay, Binding Assay

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Incubation:

Article Title: PcpA promotes higher levels of infection and modulates recruitment of myeloid-derived suppressor cells during pneumococcal pneumonia.
Article Snippet: .. The plates were coated with 2 ug/mL of total mAb in 1×PBS (Fisher Scientific, Pittsburg, PA) and incubated at 4°C overnight. ..

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: After hybridization wash, embryos were blocked with 1% blocking reagent for 1 h before incubation with rabbit anti-Klf4 antibody (1:50) diluted in 1% blocking reagent at 4°C overnight. .. After PBST (PBS + 0.1% tween 20) washes for 10 min four times, embryos were incubated with anti-rabbit Alexa-488 (1:200, Thermal Fisher Scientific) at room temperature for 3 h. Embryos were then washed with PBST and blocked with 2% blocking reagent for 1 h before incubation with anti-Digoxigenin-POD (1:500, Roche) diluted in 2% blocking reagent at 4°C overnight. .. After PBST washes, embryos were incubated with TSA-Cy3 (1:50, Perkin Elmer) diluted in Amplification buffer at 28°C for 1 h. Embryos were then washed with PBST, post fixation with 4% paraformaldehyde for 20 min, PBST washes and stored in 80% glycerol at 4°C.

Staining:

Article Title: Inhibition of JAK2/STAT3 Signaling Pathway Suppresses Proliferation of Burkitt’s Lymphoma Raji Cells via Cell Cycle Progression, Apoptosis, and Oxidative Stress by Modulating HSP70
Article Snippet: .. Detection of cell apoptosis by Hoechst 33342/PI staining Cells were washed twice with PBS buffer, fixed in PBS supplemented with 1% (wt/vol) paraformaldehyde (Fisher Scientific, Pittsburgh, PA), rinsed with tap water, and stained for 30 min with Hoechst 33342/PI (Sigma, St. Louis, MO). .. Next, the morphologic changes of the nuclei were observed under a fluorescence microscope with a 320-nm to 350-nm filter.

Article Title: A central to peripheral progression of cell cycle exit and hair cell differentiation in the developing mouse cristae
Article Snippet: For the animals sacrificed at P30, cristae were dissected from the capsule and stained as free floating whole mounts in mesh tissue baskets. .. For the BrdU antibody staining, P30 cristae were permeabilized in 0.5% Triton-X in PBS (PBSTx) for 30 minutes at room temperature (RT) and then antigen retrieval was performed using 2 M hydrochloric acid (HCl, Fisher Scientific) for 30 minutes at 37°C followed by two washes in 0.1 M sodium borate (pH 8.5, EMD Millipore) for 10 minutes to neutralize the acid. .. The cristae were then rinsed in PBS and blocked in a solution containing 10% normal donkey serum (EMD Millipore), 4% bovine serum albumin (Sigma-Aldrich), and 100 mM glycine (Fisher Scientific) in 0.5% PBSTx for 3 hours at RT.

Blocking Assay:

Article Title: Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition
Article Snippet: After hybridization wash, embryos were blocked with 1% blocking reagent for 1 h before incubation with rabbit anti-Klf4 antibody (1:50) diluted in 1% blocking reagent at 4°C overnight. .. After PBST (PBS + 0.1% tween 20) washes for 10 min four times, embryos were incubated with anti-rabbit Alexa-488 (1:200, Thermal Fisher Scientific) at room temperature for 3 h. Embryos were then washed with PBST and blocked with 2% blocking reagent for 1 h before incubation with anti-Digoxigenin-POD (1:500, Roche) diluted in 2% blocking reagent at 4°C overnight. .. After PBST washes, embryos were incubated with TSA-Cy3 (1:50, Perkin Elmer) diluted in Amplification buffer at 28°C for 1 h. Embryos were then washed with PBST, post fixation with 4% paraformaldehyde for 20 min, PBST washes and stored in 80% glycerol at 4°C.

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    Fisher Scientific pbs
    In vitro release kinetics of entrapped <t>FITC-dextran</t> from PSN microparticles in <t>PBS</t> (pH 7.4) at 37 °C. Data is shown as mean ± S.D. (n = 3).
    Pbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Fisher Scientific 1x pbs
    Insulin release from P(MAA-co-NVP) microparticles, various monomer molar feed ratios, in <t>1X</t> PBS in dynamic pH conditions (
    1x Pbs, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x pbs/product/Fisher Scientific
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x pbs - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    In vitro release kinetics of entrapped FITC-dextran from PSN microparticles in PBS (pH 7.4) at 37 °C. Data is shown as mean ± S.D. (n = 3).

    Journal: Journal of microencapsulation

    Article Title: Microparticles prepared from sulfenamide-based polymers

    doi: 10.3109/02652048.2013.814728

    Figure Lengend Snippet: In vitro release kinetics of entrapped FITC-dextran from PSN microparticles in PBS (pH 7.4) at 37 °C. Data is shown as mean ± S.D. (n = 3).

    Article Snippet: Ten mg FITC-dextran loaded microparticles were suspended in 1 mL PBS (0.01 M, pH 7.4) containing 0.1 % w/v Tween® 80 (Fischer scientific, NJ).

    Techniques: In Vitro

    Pharmacokinetics and pharmacodynamics in mice. ( A ) Pharmacokinetics of CD38 C-fusion IgG in mice. A single dose (3 mg/kg) of CD38 C-fusion IgG was administered by intravenous (IV) injection into CD-1 mice ( n = 5). Plasma concentrations of CD38 C-fusion IgG were determined by two sandwich ELISAs using the same capture antibody (Ab) [anti-human IgG (H+L)] but different detection antibodies (anti-κ light chain or anti-CD38). ( B ) Biodistribution of anti-HER2 ARC-ADC in mice. HCC1954 cells were subcutaneously implanted into the flank of female NSG mice. IRDye-labeled anti-HER2 ARC-ADC (5 mg/kg) or free IRDye at the same molar concentration was administered intravenously through tail vein 1 week after tumor implantation. Mice were then imaged at 1, 24, and 48 hours after injection, followed by euthanasia and imaging of harvested tumors and major organs. ( C ) In vivo efficacy of anti-HER2 ARC-ADC. HCC1954 cells were subcutaneously implanted into the flank of female NSG mice. Once the tumor sizes reached 100 mm 3 , mice ( n = 6) were treated with PBS or ARC-ADC (5 mg/kg) by intravenous injection (black arrows) every 3 days for a total of four times. ( D ) Body weights of mice during the in vivo efficacy study. ( E ) Ratios of major organ weight to body weight of mice at the end of in vivo efficacy study. ( F ) Kaplan-Meier survival curve for PBS- and ARC-ADC–treated groups.

    Journal: Science Advances

    Article Title: Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases

    doi: 10.1126/sciadv.aba6752

    Figure Lengend Snippet: Pharmacokinetics and pharmacodynamics in mice. ( A ) Pharmacokinetics of CD38 C-fusion IgG in mice. A single dose (3 mg/kg) of CD38 C-fusion IgG was administered by intravenous (IV) injection into CD-1 mice ( n = 5). Plasma concentrations of CD38 C-fusion IgG were determined by two sandwich ELISAs using the same capture antibody (Ab) [anti-human IgG (H+L)] but different detection antibodies (anti-κ light chain or anti-CD38). ( B ) Biodistribution of anti-HER2 ARC-ADC in mice. HCC1954 cells were subcutaneously implanted into the flank of female NSG mice. IRDye-labeled anti-HER2 ARC-ADC (5 mg/kg) or free IRDye at the same molar concentration was administered intravenously through tail vein 1 week after tumor implantation. Mice were then imaged at 1, 24, and 48 hours after injection, followed by euthanasia and imaging of harvested tumors and major organs. ( C ) In vivo efficacy of anti-HER2 ARC-ADC. HCC1954 cells were subcutaneously implanted into the flank of female NSG mice. Once the tumor sizes reached 100 mm 3 , mice ( n = 6) were treated with PBS or ARC-ADC (5 mg/kg) by intravenous injection (black arrows) every 3 days for a total of four times. ( D ) Body weights of mice during the in vivo efficacy study. ( E ) Ratios of major organ weight to body weight of mice at the end of in vivo efficacy study. ( F ) Kaplan-Meier survival curve for PBS- and ARC-ADC–treated groups.

    Article Snippet: Anti-human IgG (H+L) antibody (50-668-06) and 4% paraformaldehyde solution in PBS (AAJ19943K2) were purchased from Fisher Scientific (NH, USA).

    Techniques: Mouse Assay, IV Injection, Labeling, Concentration Assay, Tumor Implantation, Injection, Imaging, In Vivo

    Characterization of CD38-antibody fusions and 2′-Cl-araNAD + -N 3 . ( A ) Schematic of designed CD38 N-fusion IgG and CD38 C-fusion IgG. ( B ) Binding to recombinant HER2 extracellular domain by Herceptin and CD38 N- and C-fusion IgGs as analyzed by ELISA. ( C ) Enzymatic activity of CD38 catalytic domain, CD38 C-fusion IgG, and Herceptin. CD38-His 6 (20 nM), CD38 C-fusion IgG (10 nM), and Herceptin (10 nM) were incubated with NGD + (100 μM) in PBS. The CD38 cyclase activity was monitored on the basis of the formation of fluorescent cGDPR as measured at 410 nm. ( D ) Chemical structure of 2′-Cl-araNAD + -N 3 . ( E ) Inactivation of CD38 C-fusion IgG by 2′-Cl-araNAD + -N 3 . CD38 C-fusion IgG (2 nM) was incubated with NGD + (100 μM) in PBS in the presence of various concentrations of 2′-Cl-araNAD + -N 3 . The enzymatic activity was measured using cGDPR-based fluorescence assays. ( F ) Stability of Alexa Fluor 488–conjugated CD38 C-fusion IgG in mouse plasma. Using 2′-Cl-araNAD + -N 3 , CD38 C-fusion IgG was labeled with Alexa Fluor 488 and incubated in mouse plasma at 37°C for up to 14 days, followed by in-gel fluorescence imaging and Coomassie staining. The quantified fluorescence intensities for intact fusion proteins are shown at the bottom.

    Journal: Science Advances

    Article Title: Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases

    doi: 10.1126/sciadv.aba6752

    Figure Lengend Snippet: Characterization of CD38-antibody fusions and 2′-Cl-araNAD + -N 3 . ( A ) Schematic of designed CD38 N-fusion IgG and CD38 C-fusion IgG. ( B ) Binding to recombinant HER2 extracellular domain by Herceptin and CD38 N- and C-fusion IgGs as analyzed by ELISA. ( C ) Enzymatic activity of CD38 catalytic domain, CD38 C-fusion IgG, and Herceptin. CD38-His 6 (20 nM), CD38 C-fusion IgG (10 nM), and Herceptin (10 nM) were incubated with NGD + (100 μM) in PBS. The CD38 cyclase activity was monitored on the basis of the formation of fluorescent cGDPR as measured at 410 nm. ( D ) Chemical structure of 2′-Cl-araNAD + -N 3 . ( E ) Inactivation of CD38 C-fusion IgG by 2′-Cl-araNAD + -N 3 . CD38 C-fusion IgG (2 nM) was incubated with NGD + (100 μM) in PBS in the presence of various concentrations of 2′-Cl-araNAD + -N 3 . The enzymatic activity was measured using cGDPR-based fluorescence assays. ( F ) Stability of Alexa Fluor 488–conjugated CD38 C-fusion IgG in mouse plasma. Using 2′-Cl-araNAD + -N 3 , CD38 C-fusion IgG was labeled with Alexa Fluor 488 and incubated in mouse plasma at 37°C for up to 14 days, followed by in-gel fluorescence imaging and Coomassie staining. The quantified fluorescence intensities for intact fusion proteins are shown at the bottom.

    Article Snippet: Anti-human IgG (H+L) antibody (50-668-06) and 4% paraformaldehyde solution in PBS (AAJ19943K2) were purchased from Fisher Scientific (NH, USA).

    Techniques: Binding Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Activity Assay, Incubation, Fluorescence, Labeling, Imaging, Staining

    Insulin release from P(MAA-co-NVP) microparticles, various monomer molar feed ratios, in 1X PBS in dynamic pH conditions (

    Journal: Journal of biomedical materials research. Part A

    Article Title: Assessment of Poly(Methacrylic Acid-co-N-Vinyl Pyrrolidone) as a Carrier for the Oral Delivery of Therapeutic Proteins Using Caco-2 and HT29-MTX Cell Lines

    doi: 10.1002/jbm.a.32395

    Figure Lengend Snippet: Insulin release from P(MAA-co-NVP) microparticles, various monomer molar feed ratios, in 1X PBS in dynamic pH conditions (

    Article Snippet: A stock solution of bovine pancreatic insulin (Sigma-Aldrich) was prepared at a concentration of 0.5 mg/mL using 30 mg of insulin and 8 μL 0.5M ethylenediamine tetraacetic acid (EDTA) solution (Fisher Scientific, Fair Lawn, NJ) in 60 mL 1X PBS (diluted from 10X, Fisher Scientific).

    Techniques:

    Gluc clearance in HFF and HEK293 cells. Cells were incubated with 1 ml Gluc-containing medium (~3000 RLU/µl) for 1 hr, rinsed thoroughly with 1X PBS and cultured continuously for up to 1440 min (24 hr). Aliquots of cells from continuing cultures

    Journal: Molecular biotechnology

    Article Title: Secreted Luciferase for In Vivo Evaluation of Systemic Protein Delivery in Mice

    doi: 10.1007/s12033-012-9519-6

    Figure Lengend Snippet: Gluc clearance in HFF and HEK293 cells. Cells were incubated with 1 ml Gluc-containing medium (~3000 RLU/µl) for 1 hr, rinsed thoroughly with 1X PBS and cultured continuously for up to 1440 min (24 hr). Aliquots of cells from continuing cultures

    Article Snippet: Different numbers of NIH3T3-GLuc cells in 5µl of 1X PBS or 5µl of conditioned cell culture media were transferred to flat solid bottom and opaque-walled white 96 well laminator Costar plates (Fisher Scientific Inc., Pittsburg, PA).

    Techniques: Incubation, Cell Culture