pbs  (Beyotime)

 
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    Name:
    PBS
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    Catalog Number:
    c0221a
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    46.0
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    Structured Review

    Beyotime pbs

    https://www.bioz.com/result/pbs/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs - by Bioz Stars, 2021-03
    86/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3
    Article Snippet: .. Flow Cytometry Analysis Human cells were digested with trypsin and washed twice with PBS, then stained with PI (Propidium Iodide, Beyotime) according to the manufacturer’s instructions. .. Zebrafish embryos were raised to 8 dpf and fixed with 0.5% PFA at 4°C for 0.5 h. After washing with PBS, liver bud labeled by DsRed signal was dissected under a fluorescence microscope (Nikon, SMZ1000) using ultra micro tweezers; the non-liver tissues sticked to the liver were carefully removed in bright field using a fine needle.

    Cytometry:

    Article Title: Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3
    Article Snippet: .. Flow Cytometry Analysis Human cells were digested with trypsin and washed twice with PBS, then stained with PI (Propidium Iodide, Beyotime) according to the manufacturer’s instructions. .. Zebrafish embryos were raised to 8 dpf and fixed with 0.5% PFA at 4°C for 0.5 h. After washing with PBS, liver bud labeled by DsRed signal was dissected under a fluorescence microscope (Nikon, SMZ1000) using ultra micro tweezers; the non-liver tissues sticked to the liver were carefully removed in bright field using a fine needle.

    Staining:

    Article Title: Phosphorylation of Def Regulates Nucleolar p53 Turnover and Cell Cycle Progression through Def Recruitment of Calpain3
    Article Snippet: .. Flow Cytometry Analysis Human cells were digested with trypsin and washed twice with PBS, then stained with PI (Propidium Iodide, Beyotime) according to the manufacturer’s instructions. .. Zebrafish embryos were raised to 8 dpf and fixed with 0.5% PFA at 4°C for 0.5 h. After washing with PBS, liver bud labeled by DsRed signal was dissected under a fluorescence microscope (Nikon, SMZ1000) using ultra micro tweezers; the non-liver tissues sticked to the liver were carefully removed in bright field using a fine needle.

    Article Title: Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy
    Article Snippet: Subsequently, the tissue sections were rinsed for 3 times each of 5 min using PBS, then blocked by immunology staining blocking buffer (Beyotime Institute of Biotechnology) for 60 min. .. The anti-SHARPIN antibody (BD Biosciences, Franklin Lakes, NJ, USA) was diluted (1:400) with PBS (Beyotime Institute of Biotechnology) and incubated for overnight at 4°C after decanting immunology staining blocking buffer. .. Decanting the primary antibody and rinsed for 3 times were as above described, tissue sections were treated with Immunol Fluorence Staining kit with Alexa Fluor 488-Labeled Goat Anti-Rabbit IgG (Beyotime Institute of Biotechnology) at room temperature for 1 h at dark, and then stained with 300 nM 4′,6-diamidino-2-phenyindole (DAPI; Leagene, Beijing, China) for 15 min and mounted on glass slides using Anti fade Mounting Medium (Beyotime Institute of Biotechnology).

    Incubation:

    Article Title: NR2B-Tyr phosphorylation regulates synaptic plasticity in central sensitization in a chronic migraine rat model
    Article Snippet: For immunofluorescence staining, the sections were washed three times with PBS and permeabilised with 0.3% Triton X-100 (Beyotime, China) in 0.1 M PBS at 37 °C for 10 min and then incubated with 10% normal goat serum (Boster, China) at 37 °C for 30 min, using a neuronal marker (anti-neuronal nuclei (NeuN), mouse, 1:200, Novus), anti-PSD95 (1:500, rabbit, Abcam), anti-synaptophysin (1:500, rabbit, Abcam), anti-synaptotagmin1 (1:200, rabbit, Bioss), anti-CGRP (1:50, mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-SP (1:50, mouse, Abcam) at 4 °C overnight. .. Then, after three washes with PBS, the sections were incubated with secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin G (IgG, 1:200, Beyotime, China), Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200, Beyotime, China), and Cy3-conjugated goat anti-mouse IgG (1:200, Beyotime, China) at 37 °C for 90 min. Microphotographs were obtained analysed with a fluorescent confocal microscope (ZEISS, Germany). .. PBS rather than primary antibody was applied to the negative control sections, and no positive signals were detected.

    Article Title: Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro
    Article Snippet: .. Then cells were incubated with rat anti-HcESP IgG (1:100) as a primary antibody for 2 h. After being washed with PBS for 3 times (5 min each), the cells were incubated with second antibody (1:300) coupled to the fluorescent dye Cy3 (Beyotime, Jiangsu, China) for 1h at room temperature. .. The nuclear staining was performed with 2-(4-amidinophenyl)-6-indole carbamidinedihydrochloride (DAPI, 1.5 μM; Sigma, MO, USA for 6 min. finally the protein localization was determined by checking the staining patterns with a 100× oil objective lens on a laser scanning confocal microscope (L SM710, Zeiss, Jena, Germany).

    Article Title: Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy
    Article Snippet: Subsequently, the tissue sections were rinsed for 3 times each of 5 min using PBS, then blocked by immunology staining blocking buffer (Beyotime Institute of Biotechnology) for 60 min. .. The anti-SHARPIN antibody (BD Biosciences, Franklin Lakes, NJ, USA) was diluted (1:400) with PBS (Beyotime Institute of Biotechnology) and incubated for overnight at 4°C after decanting immunology staining blocking buffer. .. Decanting the primary antibody and rinsed for 3 times were as above described, tissue sections were treated with Immunol Fluorence Staining kit with Alexa Fluor 488-Labeled Goat Anti-Rabbit IgG (Beyotime Institute of Biotechnology) at room temperature for 1 h at dark, and then stained with 300 nM 4′,6-diamidino-2-phenyindole (DAPI; Leagene, Beijing, China) for 15 min and mounted on glass slides using Anti fade Mounting Medium (Beyotime Institute of Biotechnology).

    Microscopy:

    Article Title: NR2B-Tyr phosphorylation regulates synaptic plasticity in central sensitization in a chronic migraine rat model
    Article Snippet: For immunofluorescence staining, the sections were washed three times with PBS and permeabilised with 0.3% Triton X-100 (Beyotime, China) in 0.1 M PBS at 37 °C for 10 min and then incubated with 10% normal goat serum (Boster, China) at 37 °C for 30 min, using a neuronal marker (anti-neuronal nuclei (NeuN), mouse, 1:200, Novus), anti-PSD95 (1:500, rabbit, Abcam), anti-synaptophysin (1:500, rabbit, Abcam), anti-synaptotagmin1 (1:200, rabbit, Bioss), anti-CGRP (1:50, mouse, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-SP (1:50, mouse, Abcam) at 4 °C overnight. .. Then, after three washes with PBS, the sections were incubated with secondary antibodies Alexa Fluor 488-conjugated goat anti-rabbit immunoglobulin G (IgG, 1:200, Beyotime, China), Alexa Fluor 488-conjugated goat anti-mouse IgG (1:200, Beyotime, China), and Cy3-conjugated goat anti-mouse IgG (1:200, Beyotime, China) at 37 °C for 90 min. Microphotographs were obtained analysed with a fluorescent confocal microscope (ZEISS, Germany). .. PBS rather than primary antibody was applied to the negative control sections, and no positive signals were detected.

    Western Blot:

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. Western Blot Analyses 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Article Title: Alternol, a natural compound, exerts an anti‐tumour effect on osteosarcoma by modulating of STAT3 and ROS/ MAPK signalling pathways
    Article Snippet: .. Western blot assay Cells were washed twice with cold 1× PBS solution, lysed with RIPA lysis Buffer (Beyotime) with phosphorylase and protease inhibitor (Beyotime). .. Protein concentrations were determined with Standard BCA Protein Assay Kit (Beyotime).

    Radio Immunoprecipitation:

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. Western Blot Analyses 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Lysis:

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. Western Blot Analyses 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Article Title: Alternol, a natural compound, exerts an anti‐tumour effect on osteosarcoma by modulating of STAT3 and ROS/ MAPK signalling pathways
    Article Snippet: .. Western blot assay Cells were washed twice with cold 1× PBS solution, lysed with RIPA lysis Buffer (Beyotime) with phosphorylase and protease inhibitor (Beyotime). .. Protein concentrations were determined with Standard BCA Protein Assay Kit (Beyotime).

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Protease Inhibitor:

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. Western Blot Analyses 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Article Title: Alternol, a natural compound, exerts an anti‐tumour effect on osteosarcoma by modulating of STAT3 and ROS/ MAPK signalling pathways
    Article Snippet: .. Western blot assay Cells were washed twice with cold 1× PBS solution, lysed with RIPA lysis Buffer (Beyotime) with phosphorylase and protease inhibitor (Beyotime). .. Protein concentrations were determined with Standard BCA Protein Assay Kit (Beyotime).

    Article Title: Sarcoma-Targeting Peptide-Decorated Polypeptide Nanogel Intracellularly Delivers Shikonin for Upregulated Osteosarcoma Necroptosis and Diminished Pulmonary Metastasis
    Article Snippet: .. 143B cells were treated with 2.0 μΜ SHK, NG/SHK, or STP-NG/SHK for 24 h. Cells were washed twice with PBS solution and lysed with radioimmunoprecipitation assay (RIPA) lysis buffer (Beyotime Institute of Biotechnology, Beijing, P. R. China) and protease inhibitor (Thermo Scientific, Waltham, USA). ..

    Blocking Assay:

    Article Title: Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy
    Article Snippet: Subsequently, the tissue sections were rinsed for 3 times each of 5 min using PBS, then blocked by immunology staining blocking buffer (Beyotime Institute of Biotechnology) for 60 min. .. The anti-SHARPIN antibody (BD Biosciences, Franklin Lakes, NJ, USA) was diluted (1:400) with PBS (Beyotime Institute of Biotechnology) and incubated for overnight at 4°C after decanting immunology staining blocking buffer. .. Decanting the primary antibody and rinsed for 3 times were as above described, tissue sections were treated with Immunol Fluorence Staining kit with Alexa Fluor 488-Labeled Goat Anti-Rabbit IgG (Beyotime Institute of Biotechnology) at room temperature for 1 h at dark, and then stained with 300 nM 4′,6-diamidino-2-phenyindole (DAPI; Leagene, Beijing, China) for 15 min and mounted on glass slides using Anti fade Mounting Medium (Beyotime Institute of Biotechnology).

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  • 86
    Beyotime phosphate buffer saline pbs
    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with <t>PBS</t> and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . <t>UVA,</t> c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )
    Phosphate Buffer Saline Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffer saline pbs/product/Beyotime
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffer saline pbs - by Bioz Stars, 2021-03
    86/100 stars
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    97
    Beyotime pbs medium
    Percentage of BrdU positive eosinophils of <t>BALF,</t> blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or <t>PBS</t> (control) (*p
    Pbs Medium, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs medium/product/Beyotime
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs medium - by Bioz Stars, 2021-03
    97/100 stars
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    93
    Beyotime phosphate buffered solution pbs
    Reducing Ezrin suppressed metastasis of <t>EGF-injected</t> MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; <t>Con.+PBS,</t> tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P
    Phosphate Buffered Solution Pbs, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphate buffered solution pbs/product/Beyotime
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphate buffered solution pbs - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Journal: Journal of Biological Engineering

    Article Title: Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

    doi: 10.1186/s13036-018-0099-2

    Figure Lengend Snippet: Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Article Snippet: In brief, following UVA irradiation, cells are washed twice in phosphate buffer saline (PBS) and allowed to incubate in 10 μM/L DCFH-DA (1:1000 dilution in PBS) at 37 °C for 20 min according to the manufacturer’s instructions (Beyotime Inc.).

    Techniques: Modification, Irradiation

    Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Journal: Journal of Biological Engineering

    Article Title: Label-free electrochemical sensor to investigate the effect of tocopherol on generation of superoxide ions following UV irradiation

    doi: 10.1186/s13036-018-0099-2

    Figure Lengend Snippet: Calibration of CNT@DNA-Mn 3 (PO 4 ) 2 modified glass-carbon electrode for measurement of O 2 •− and electrochemical characterization of O 2 •− released by cells following UVR irradiation. a Cyclic voltammetry (CV) curves measured with PBS and PBS plus different concentrations of KO 2 (nM). a : 0, b : 5, c : 10, d : 20, e : 40, f : 60, g : 80, h : 100, i : 150, j : 200; inset: CV curves measured with PBS (black), PBS plus KO 2 (150 nM, blue), and KO 2 solution added with SOD (300 U mL − 1 , red). b Calibration curve for serial concentrations of O 2 •− . c O 2 •− CV curves of cells under irradiation, a. UV, b . UVA, c . UVB, d . Sham-irradiation control. d Histogram of peak current changes compared to sham-irradiated cells (black column, n = 3) and generation of O 2 •− upon irradiation with UV as quantified by the O 2 •− assay kit: increase of absorbance at 450 nm (grey column, n = 3). SOD: superoxide dismutase. UV (10.5 J cm − 2 = 105 kJ m − 2 ), UVA (10 J cm − 2 = 100 kJ m − 2 ), UVB (0.5 J cm − 2 = 5 kJ m − 2 )

    Article Snippet: In brief, following UVA irradiation, cells are washed twice in phosphate buffer saline (PBS) and allowed to incubate in 10 μM/L DCFH-DA (1:1000 dilution in PBS) at 37 °C for 20 min according to the manufacturer’s instructions (Beyotime Inc.).

    Techniques: Modification, Irradiation

    Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Journal: Clinical and Translational Allergy

    Article Title: Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

    doi: 10.1186/s13601-018-0190-2

    Figure Lengend Snippet: Percentage of BrdU positive eosinophils of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Article Snippet: Measurement of eosinophil number in BALF Cells were seeded in PBS medium (Beyotime Institute of Biotechnology, Haimen, China) at 1 × 105 cells/mL and stained with Fast Wright and Giemsa Stain kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China), according to manufacturer’s protocol.

    Techniques:

    Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Journal: Clinical and Translational Allergy

    Article Title: Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma

    doi: 10.1186/s13601-018-0190-2

    Figure Lengend Snippet: Eosinophil percentage of BALF, blood and bone marrow from wild type and IL-25 KO mouse models that were sensitized and challenged with OVA or PBS (control) (*p

    Article Snippet: Measurement of eosinophil number in BALF Cells were seeded in PBS medium (Beyotime Institute of Biotechnology, Haimen, China) at 1 × 105 cells/mL and stained with Fast Wright and Giemsa Stain kit (Nanjing Jiancheng Technology Co., Ltd., Nanjing, China), according to manufacturer’s protocol.

    Techniques:

    Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma

    doi: 10.12659/MSM.906945

    Figure Lengend Snippet: Reducing Ezrin suppressed metastasis of EGF-injected MG63 and U20S xenografts of BALB/c-nu mice. ( A ) BALB/c-mu mouse with osteosarcoma xenografts. ( B ) Liver and lung weights of tumorigenic mice. ( C ) HPRT mRNA level in livers and lungs of tumorigenic mice. ( D ) E-cadherin, vimentin, and p65 protein levels in livers and lungs of tumorigenic mice. (HPRT, hypoxanthine guanine phosphoribosyl transferase; Con.+PBS, tumor xenografts and PBS injection; siRNA+PBS, tumor xenografts with silencing Ezrin and PBS injection; Con.+EGF, tumor xenografts and EGF injection; siRNA+EGF, tumor xenografts with silencing Ezrin and EGF injection; * compared to Con.+PBS, P

    Article Snippet: When the mice developed palpable xenografts (more than 0.5 cm diameter), we injected the tumor with phosphate-buffered solution (PBS) (Beyotime) or EGF (0.02 mg/kg) twice a week for 5 weeks.

    Techniques: Injection, Mouse Assay