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Agilent technologies pbs
Pbs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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pbs - by Bioz Stars, 2021-03
86/100 stars

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Article Title: Enhanced Open-Circuit Voltage of PbS Nanocrystal Quantum Dot Solar Cells
Article Snippet: Electrical characterization of devices The current density–voltage (J −V ) measurements were performed with a parameter analyzer (Agilent 4156C) in air without any encapsulation under the spectral output from a 150 W solar simulator (Newport Corporation ) using an AM 1.5 G filter.

Immunohistochemistry:

Article Title: Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
Article Snippet: .. Immunohistochemistry For immunofluorescence staining of tissues, paraffin sections were deparaffinized, and antigens were retrieved by boiling in Dako Antigen Retrieval Agent pH 9 (Dako) for 20 min. After PBS wash, blocking was performed for 1 h with 5% BSA in PBS/PBS++ or Dako Antibody Diluent at RT. .. Primary antibodies diluted in either AB buffer or Dako Antibody Diluent were applied O/N at 4 °C in a humidified chamber.

Immunofluorescence:

Article Title: Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
Article Snippet: .. Immunohistochemistry For immunofluorescence staining of tissues, paraffin sections were deparaffinized, and antigens were retrieved by boiling in Dako Antigen Retrieval Agent pH 9 (Dako) for 20 min. After PBS wash, blocking was performed for 1 h with 5% BSA in PBS/PBS++ or Dako Antibody Diluent at RT. .. Primary antibodies diluted in either AB buffer or Dako Antibody Diluent were applied O/N at 4 °C in a humidified chamber.

Staining:

Article Title: Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
Article Snippet: .. Immunohistochemistry For immunofluorescence staining of tissues, paraffin sections were deparaffinized, and antigens were retrieved by boiling in Dako Antigen Retrieval Agent pH 9 (Dako) for 20 min. After PBS wash, blocking was performed for 1 h with 5% BSA in PBS/PBS++ or Dako Antibody Diluent at RT. .. Primary antibodies diluted in either AB buffer or Dako Antibody Diluent were applied O/N at 4 °C in a humidified chamber.

Blocking Assay:

Article Title: Polarity signaling ensures epidermal homeostasis by coupling cellular mechanics and genomic integrity
Article Snippet: .. Immunohistochemistry For immunofluorescence staining of tissues, paraffin sections were deparaffinized, and antigens were retrieved by boiling in Dako Antigen Retrieval Agent pH 9 (Dako) for 20 min. After PBS wash, blocking was performed for 1 h with 5% BSA in PBS/PBS++ or Dako Antibody Diluent at RT. .. Primary antibodies diluted in either AB buffer or Dako Antibody Diluent were applied O/N at 4 °C in a humidified chamber.

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  • 97
    Agilent technologies pbs
    Treatment of 005 GSC-derived tumors with systemic immune checkpoint inhibitors, intratumoral G47 Δ-mIL12, or the combination prolongs survival (A–B) Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with G47Δ-mIL12 (5 × 10 5 pfu) or <t>PBS</t> injected IT on day 12 (upward arrow) and isotype control IgG (10 mg/kg), anti-(α)PD-L1 antibody (A) , or anti-(α)PD-1 antibody (B) injected IP on days 15, 18 and 21 (downward arrows). Values from a single experiment, with Mock (treated with PBS and IgG) and G47Δ-mIL12 groups the same in A and B. Median survival of Mock (33.5 days; n=6) was compared to anti-PD-1 (39 days; n=8, p=0.02), anti-PD-L1 (42 days; n=7, p=0.003), or G47Δ-mIL12 (39 days; n=8, p=0.01) by Log-rank analysis. Similarly, G47Δ-mIL12 was compared to the combination of G47Δ-mIL12 with anti-PD-1 (49 days; n=7, p=0.02) or -PD-L1 (50 days; n=7, p=0.03), and antibodies were compared to the combination of G47Δ-mIL12 with anti-PD-1 (p=0.053) or anti-PD-L1 (p=0.08). Experiment was conducted once (A) or twice (B). C. Mice implanted with 2 × 10 4 005 GSCs on day 0 and treated with G47Δ-mIL12 or PBS injected IT on day 8 and anti-(α)CTLA-4 antibody or isotype control IgG (5 mg/kg) injected IP on days 8, 11 and 14 (n=8/group, except for G47Δ-mIL12 n=7). Median survival of Mock (37.5 days) was compared to anti-CTLA-4 (45 days; p=0.002) or G47Δ-mIL12 (40 days; p=0.03) alone by Log-rank analysis. Similarly, combination of G47Δ-mIL12 with anti-CTLA-4 (58 days) was compared to anti-CTLA-4 (p=0.05) or G47Δ-mIL12 (p=0.008). Experiment was conducted 2 times. D. Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with PBS (right; n=2), rat anti-(α)PD-1 antibody (middle; 200 μg/mouse; n=2), or rat anti-(α)PD-L1 antibody (left; 200 μg/mouse; n=2) injected IP on day 25, and sacrificed 3 hr later. Antibodies were detected with <t>HRP-conjugated</t> anti-(α)rat Ig (brown; right) or control HRP-conjugated anti-(α)rabbit Ig (left). * normal brain adjacent to tumor. Scale bar=100 μm. E. 005 GSCs (1.5 × 10 5 ) implanted on day 0, treated with PBS or G47Δ-mIL12 (5 × 10 5 .
    Pbs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
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    pbs - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Agilent technologies uv irradiated mhv68 virus stock pbs
    B cell-intrinsic IRF-1 deficiency selectively attenuates <t>MHV68-driven</t> germinal center response. (A to D) CD19 Cre-positive and Cre-negative littermates were intravenously infected with 2 × 10 6 PFU of LCMV clone 13 (CL13). Spleens were collected at 14 days postinfection and processed into a single-cell suspension, and the germinal center response was measured with germinal center B cells (A and B) defined as B220 + GL7 + CD95 + cells and T follicular helper cells (C and D) defined as CD3 + CD4 + CXCR5 + PD-1 + cells. (E and F) Abundance of LCMV-specific IgG (E) and IgM (F) antibodies in the sera. Each symbol represents an individual spleen. P values are as indicated. **, P
    Uv Irradiated Mhv68 Virus Stock Pbs, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    97
    Agilent technologies anti cd8 pb
    NY-ESO-1-specific proliferation, cytokine expression and CD107a expression of T cells with no alloreactive immune responses. ( A ) CD4 + and <t>CD8</t> + T cells show a specific proliferation in response to NY-ESO-1. T cells of the final in vitro expanded T-cell
    Anti Cd8 Pb, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Treatment of 005 GSC-derived tumors with systemic immune checkpoint inhibitors, intratumoral G47 Δ-mIL12, or the combination prolongs survival (A–B) Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with G47Δ-mIL12 (5 × 10 5 pfu) or PBS injected IT on day 12 (upward arrow) and isotype control IgG (10 mg/kg), anti-(α)PD-L1 antibody (A) , or anti-(α)PD-1 antibody (B) injected IP on days 15, 18 and 21 (downward arrows). Values from a single experiment, with Mock (treated with PBS and IgG) and G47Δ-mIL12 groups the same in A and B. Median survival of Mock (33.5 days; n=6) was compared to anti-PD-1 (39 days; n=8, p=0.02), anti-PD-L1 (42 days; n=7, p=0.003), or G47Δ-mIL12 (39 days; n=8, p=0.01) by Log-rank analysis. Similarly, G47Δ-mIL12 was compared to the combination of G47Δ-mIL12 with anti-PD-1 (49 days; n=7, p=0.02) or -PD-L1 (50 days; n=7, p=0.03), and antibodies were compared to the combination of G47Δ-mIL12 with anti-PD-1 (p=0.053) or anti-PD-L1 (p=0.08). Experiment was conducted once (A) or twice (B). C. Mice implanted with 2 × 10 4 005 GSCs on day 0 and treated with G47Δ-mIL12 or PBS injected IT on day 8 and anti-(α)CTLA-4 antibody or isotype control IgG (5 mg/kg) injected IP on days 8, 11 and 14 (n=8/group, except for G47Δ-mIL12 n=7). Median survival of Mock (37.5 days) was compared to anti-CTLA-4 (45 days; p=0.002) or G47Δ-mIL12 (40 days; p=0.03) alone by Log-rank analysis. Similarly, combination of G47Δ-mIL12 with anti-CTLA-4 (58 days) was compared to anti-CTLA-4 (p=0.05) or G47Δ-mIL12 (p=0.008). Experiment was conducted 2 times. D. Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with PBS (right; n=2), rat anti-(α)PD-1 antibody (middle; 200 μg/mouse; n=2), or rat anti-(α)PD-L1 antibody (left; 200 μg/mouse; n=2) injected IP on day 25, and sacrificed 3 hr later. Antibodies were detected with HRP-conjugated anti-(α)rat Ig (brown; right) or control HRP-conjugated anti-(α)rabbit Ig (left). * normal brain adjacent to tumor. Scale bar=100 μm. E. 005 GSCs (1.5 × 10 5 ) implanted on day 0, treated with PBS or G47Δ-mIL12 (5 × 10 5 .

    Journal: Cancer cell

    Article Title: MACROPHAGE POLARIZATION CONTRIBUTES TO GLIOBLASTOMA ERADICATION BY COMBINATION IMMUNOVIROTHERAPY AND IMMUNE CHECKPOINT BLOCKADE

    doi: 10.1016/j.ccell.2017.07.006

    Figure Lengend Snippet: Treatment of 005 GSC-derived tumors with systemic immune checkpoint inhibitors, intratumoral G47 Δ-mIL12, or the combination prolongs survival (A–B) Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with G47Δ-mIL12 (5 × 10 5 pfu) or PBS injected IT on day 12 (upward arrow) and isotype control IgG (10 mg/kg), anti-(α)PD-L1 antibody (A) , or anti-(α)PD-1 antibody (B) injected IP on days 15, 18 and 21 (downward arrows). Values from a single experiment, with Mock (treated with PBS and IgG) and G47Δ-mIL12 groups the same in A and B. Median survival of Mock (33.5 days; n=6) was compared to anti-PD-1 (39 days; n=8, p=0.02), anti-PD-L1 (42 days; n=7, p=0.003), or G47Δ-mIL12 (39 days; n=8, p=0.01) by Log-rank analysis. Similarly, G47Δ-mIL12 was compared to the combination of G47Δ-mIL12 with anti-PD-1 (49 days; n=7, p=0.02) or -PD-L1 (50 days; n=7, p=0.03), and antibodies were compared to the combination of G47Δ-mIL12 with anti-PD-1 (p=0.053) or anti-PD-L1 (p=0.08). Experiment was conducted once (A) or twice (B). C. Mice implanted with 2 × 10 4 005 GSCs on day 0 and treated with G47Δ-mIL12 or PBS injected IT on day 8 and anti-(α)CTLA-4 antibody or isotype control IgG (5 mg/kg) injected IP on days 8, 11 and 14 (n=8/group, except for G47Δ-mIL12 n=7). Median survival of Mock (37.5 days) was compared to anti-CTLA-4 (45 days; p=0.002) or G47Δ-mIL12 (40 days; p=0.03) alone by Log-rank analysis. Similarly, combination of G47Δ-mIL12 with anti-CTLA-4 (58 days) was compared to anti-CTLA-4 (p=0.05) or G47Δ-mIL12 (p=0.008). Experiment was conducted 2 times. D. Mice implanted with 2 × 10 4 005 GSCs on day 0, treated with PBS (right; n=2), rat anti-(α)PD-1 antibody (middle; 200 μg/mouse; n=2), or rat anti-(α)PD-L1 antibody (left; 200 μg/mouse; n=2) injected IP on day 25, and sacrificed 3 hr later. Antibodies were detected with HRP-conjugated anti-(α)rat Ig (brown; right) or control HRP-conjugated anti-(α)rabbit Ig (left). * normal brain adjacent to tumor. Scale bar=100 μm. E. 005 GSCs (1.5 × 10 5 ) implanted on day 0, treated with PBS or G47Δ-mIL12 (5 × 10 5 .

    Article Snippet: Ten μm sections were air-dried, followed by wash and rehydration in PBS, incubation with 3% H2 O2 , washed in PBS, incubated with 5% bovine serum albumin and 10% goat serum, and then with HRP-conjugated anti-rat Ig, control HRP-conjugated anti-rabbit Ig, or PBS, followed by DAB staining (DAKO).

    Techniques: Derivative Assay, Mouse Assay, Injection

    Apoptotic response. Caspase-3 activity ( A ) and Poly ADP ribose polymerase (PARP) ( B ) in the lungs of C57BL/6 (B6) or Fas-deficient LPR mice treated with intratracheal installations of either PBS or E. coli LPS, 15 ng/kg, followed by either spontaneous breathing (SP) or four hours of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram. Caspase-3 activity was significantly higher in the lpr mice exposed to MV + LPS. n = at least 6/group. Double-labeling for TUNEL (green) and cytokeratin (red) reveals that the TUNEL positive cells are located in the alveolar wall, but most of them are cytokeratin negative.

    Journal: Respiratory Research

    Article Title: Fas-deficient mice have impaired alveolar neutrophil recruitment and decreased expression of anti-KC autoantibody:KC complexes in a model of acute lung injury

    doi: 10.1186/1465-9921-13-91

    Figure Lengend Snippet: Apoptotic response. Caspase-3 activity ( A ) and Poly ADP ribose polymerase (PARP) ( B ) in the lungs of C57BL/6 (B6) or Fas-deficient LPR mice treated with intratracheal installations of either PBS or E. coli LPS, 15 ng/kg, followed by either spontaneous breathing (SP) or four hours of mechanical ventilation (MV) with tidal volumes of 10 mL per kilogram. Caspase-3 activity was significantly higher in the lpr mice exposed to MV + LPS. n = at least 6/group. Double-labeling for TUNEL (green) and cytokeratin (red) reveals that the TUNEL positive cells are located in the alveolar wall, but most of them are cytokeratin negative.

    Article Snippet: Immediately after TUNEL labeling the slides were washed three times in PBS, blocked with Protein Block (Dako, Carpinteria CA) and incubated for 2 hr in the dark at 37°C with the mouse monoclonal pan-cytokeratin antibody C11 (Abcam, Cambridge, UK) previously labeled with Alexa Fluor 555 (Invitrogen, Eugene, OR).

    Techniques: Activity Assay, Mouse Assay, Labeling, TUNEL Assay

    B cell-intrinsic IRF-1 deficiency selectively attenuates MHV68-driven germinal center response. (A to D) CD19 Cre-positive and Cre-negative littermates were intravenously infected with 2 × 10 6 PFU of LCMV clone 13 (CL13). Spleens were collected at 14 days postinfection and processed into a single-cell suspension, and the germinal center response was measured with germinal center B cells (A and B) defined as B220 + GL7 + CD95 + cells and T follicular helper cells (C and D) defined as CD3 + CD4 + CXCR5 + PD-1 + cells. (E and F) Abundance of LCMV-specific IgG (E) and IgM (F) antibodies in the sera. Each symbol represents an individual spleen. P values are as indicated. **, P

    Journal: Journal of Virology

    Article Title: B Cell-Intrinsic Expression of Interferon Regulatory Factor 1 Supports Chronic Murine Gammaherpesvirus 68 Infection

    doi: 10.1128/JVI.00399-20

    Figure Lengend Snippet: B cell-intrinsic IRF-1 deficiency selectively attenuates MHV68-driven germinal center response. (A to D) CD19 Cre-positive and Cre-negative littermates were intravenously infected with 2 × 10 6 PFU of LCMV clone 13 (CL13). Spleens were collected at 14 days postinfection and processed into a single-cell suspension, and the germinal center response was measured with germinal center B cells (A and B) defined as B220 + GL7 + CD95 + cells and T follicular helper cells (C and D) defined as CD3 + CD4 + CXCR5 + PD-1 + cells. (E and F) Abundance of LCMV-specific IgG (E) and IgM (F) antibodies in the sera. Each symbol represents an individual spleen. P values are as indicated. **, P

    Article Snippet: Briefly, Nunc MaxiSorp plates (Fisher Scientific, Pittsburgh, PA) were coated with either anti-mouse IgG antibodies (heavy plus light chain) or anti-mouse IgM antibodies (total IgG and IgM levels) (Jackson ImmunoResearch, West Grove, PA), UV-irradiated MHV68 virus stock–PBS (Stratalinker UV Crosslinker 1800; Agilent Technologies, Santa Clara, CA) (740,000 μJ/cm2 × 2), LCMV clone 13-infected BHK cell lysates, or dsDNA from Escherichia coli (12.5 μg/ml; Sigma-Aldrich, St. Louis, MO) overnight at 4°C.

    Techniques: Infection

    NY-ESO-1-specific proliferation, cytokine expression and CD107a expression of T cells with no alloreactive immune responses. ( A ) CD4 + and CD8 + T cells show a specific proliferation in response to NY-ESO-1. T cells of the final in vitro expanded T-cell

    Journal: Oncoimmunology

    Article Title: Rapid generation of NY-ESO-1-specific CD4+ THELPER1 cells for adoptive T-cell therapy

    doi: 10.1080/2162402X.2014.1002723

    Figure Lengend Snippet: NY-ESO-1-specific proliferation, cytokine expression and CD107a expression of T cells with no alloreactive immune responses. ( A ) CD4 + and CD8 + T cells show a specific proliferation in response to NY-ESO-1. T cells of the final in vitro expanded T-cell

    Article Snippet: Flow cytometry of cells was performed using the following antibodies: anti-CD3 BV-510, anti-CD56-BV711, anti-CD45RO-BV785 (Biolegend), anti-CD4-AF-700 (Exbio), anti-CD3-PE-Cy7 (clone SK7), anti-CD4-APC-H7, anti-CD4-PerCP, anti-CD8-FITC, anti-CD8-APC-H7, anti-CD3-APC, anti-CD45RA-FITC, anti-CD45RO-PE, anti-CD27-FITC, anti-CD27-PE-CF594, anti-CD107a-APC, anti-IFN-γ-APC, anti-IFN-γ-PE (all BD Bioscience) anti-CD62L-FITC, anti-CD28-PE, anti-CD80-PE, anti-CD83-FITC (PharMingen BD), anti-CD3-FITC (clone MEM 57) anti-CD25-PE (both Exbio) anti-CD8-PB (Dako), anti-CCR7-PE (R & D Systems), anti-TNFα-APC, anti-CD28-PECy7 (eBioscience), anti-IFNγ-PE, anti-IL-10-APC (both Miltenyi Biotech).

    Techniques: Expressing, In Vitro