Structured Review

Thermo Fisher pbs t
Ser-266 is a novel phosphorylation site regulated by cAMP signaling. A , different amounts of regular peptide ( top ) or phosphopeptide ( bottom ) were spotted on a nitrocellulose membrane, air-dried, blocked for 2 h in <t>PBS-T</t> containing 20% horse serum, and
Pbs T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t/product/Thermo Fisher
Average 95 stars, based on 3038 article reviews
Price from $9.99 to $1999.99
pbs t - by Bioz Stars, 2020-07
95/100 stars

Images

1) Product Images from "Dephosphorylation at a Conserved SP Motif Governs cAMP Sensitivity and Nuclear Localization of Class IIa Histone Deacetylases *"

Article Title: Dephosphorylation at a Conserved SP Motif Governs cAMP Sensitivity and Nuclear Localization of Class IIa Histone Deacetylases *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M112.445668

Ser-266 is a novel phosphorylation site regulated by cAMP signaling. A , different amounts of regular peptide ( top ) or phosphopeptide ( bottom ) were spotted on a nitrocellulose membrane, air-dried, blocked for 2 h in PBS-T containing 20% horse serum, and
Figure Legend Snippet: Ser-266 is a novel phosphorylation site regulated by cAMP signaling. A , different amounts of regular peptide ( top ) or phosphopeptide ( bottom ) were spotted on a nitrocellulose membrane, air-dried, blocked for 2 h in PBS-T containing 20% horse serum, and

Techniques Used:

2) Product Images from "A Single-Stranded DNA Aptamer That Selectively Binds to Staphylococcus aureus Enterotoxin B"

Article Title: A Single-Stranded DNA Aptamer That Selectively Binds to Staphylococcus aureus Enterotoxin B

Journal: PLoS ONE

doi: 10.1371/journal.pone.0033410

APT SEB1 binds to SEB, but not BSA. Aptamer-precipitation of SEB from 10-fold excess of BSA using several DNA sequences was visualized by 4–12% SDS-PAGE with silver stain. Dynabeads® M-270 Streptavidin magnetic beads coated with APT SEB1 (lane 3), random 78-base ssDNA (lanes 4–6), PCR forward primer (used in this study, lane 7), and nothing (lane 8), were incubated in 500 µl PBS-T incurred with 10 µg BSA and 1 µg SEB. After washing the Dynabeads with PBS-T, the protein eluate (lanes 3–8) was loaded onto the SDS-PAGE gel. Lanes 1 and 2 contain 50 ng of standard BSA and SEB, respectively. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.
Figure Legend Snippet: APT SEB1 binds to SEB, but not BSA. Aptamer-precipitation of SEB from 10-fold excess of BSA using several DNA sequences was visualized by 4–12% SDS-PAGE with silver stain. Dynabeads® M-270 Streptavidin magnetic beads coated with APT SEB1 (lane 3), random 78-base ssDNA (lanes 4–6), PCR forward primer (used in this study, lane 7), and nothing (lane 8), were incubated in 500 µl PBS-T incurred with 10 µg BSA and 1 µg SEB. After washing the Dynabeads with PBS-T, the protein eluate (lanes 3–8) was loaded onto the SDS-PAGE gel. Lanes 1 and 2 contain 50 ng of standard BSA and SEB, respectively. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.

Techniques Used: SDS Page, Silver Staining, Magnetic Beads, Polymerase Chain Reaction, Incubation, Labeling

APT SEB1 is selective for SEB within a complex mixture. The toxin-rich cell-free culture supernatant from four S. aureus strains was assayed for the presence of SEB by aptamer-precipitation. Five microliters of each culture supernatant was loaded onto an 4–12% SDS-PAGE gel to determine the protein content (lanes 2, 4, 6, 8). Three milliliters of each culture supernatant was incubated with APT SEB1 -coated Dynabeads. After washing with PBS-T, the resultant protein eluate from the APT SEB1 -coated Dynabeads was analyzed (lanes 3, 5, 7, 9). By PCR and ELISA analysis (Sandra Tallent, personal communication) the four strains potentially express a total of 17 enterotoxins and toxic shock syndrome toxin. However, only strain BAA1747 contains the gene for SEB. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.
Figure Legend Snippet: APT SEB1 is selective for SEB within a complex mixture. The toxin-rich cell-free culture supernatant from four S. aureus strains was assayed for the presence of SEB by aptamer-precipitation. Five microliters of each culture supernatant was loaded onto an 4–12% SDS-PAGE gel to determine the protein content (lanes 2, 4, 6, 8). Three milliliters of each culture supernatant was incubated with APT SEB1 -coated Dynabeads. After washing with PBS-T, the resultant protein eluate from the APT SEB1 -coated Dynabeads was analyzed (lanes 3, 5, 7, 9). By PCR and ELISA analysis (Sandra Tallent, personal communication) the four strains potentially express a total of 17 enterotoxins and toxic shock syndrome toxin. However, only strain BAA1747 contains the gene for SEB. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.

Techniques Used: SDS Page, Incubation, Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Labeling

APT SEB1 is selective for SEB but not other closely related enterotoxins. Aptamer-precipitation of SEB from a mixture of enterotoxins was visualized by 4–12% SDS-PAGE with silver stain. Dynabeads® M-270 Streptavidin magnetic beads coated with APT SEB1 were incubated in 1000 µl PBS-T incurred with 1 µg each of SEA, SEC 1 , SEC 2 , SEC 3 , SED, and SEE. The aptamer-precipitation was carried out either with (lane 3) or without (lane 4) 1 µg SEB present in the mixture. After washing the Dynabeads with PBS-T, the protein eluate (lanes 3–8) was loaded onto the SDS-PAGE gel. Lanes 1 and 2 contain 200 ng of standard BSA and SEB, respectively. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.
Figure Legend Snippet: APT SEB1 is selective for SEB but not other closely related enterotoxins. Aptamer-precipitation of SEB from a mixture of enterotoxins was visualized by 4–12% SDS-PAGE with silver stain. Dynabeads® M-270 Streptavidin magnetic beads coated with APT SEB1 were incubated in 1000 µl PBS-T incurred with 1 µg each of SEA, SEC 1 , SEC 2 , SEC 3 , SED, and SEE. The aptamer-precipitation was carried out either with (lane 3) or without (lane 4) 1 µg SEB present in the mixture. After washing the Dynabeads with PBS-T, the protein eluate (lanes 3–8) was loaded onto the SDS-PAGE gel. Lanes 1 and 2 contain 200 ng of standard BSA and SEB, respectively. The protein bands labeled as “SA” represent the monomer of streptavidin liberated by the elution protocol.

Techniques Used: SDS Page, Silver Staining, Magnetic Beads, Incubation, Size-exclusion Chromatography, Labeling

3) Product Images from "A Coproantigen Diagnostic Test for Strongyloides Infection"

Article Title: A Coproantigen Diagnostic Test for Strongyloides Infection

Journal: PLoS Neglected Tropical Diseases

doi: 10.1371/journal.pntd.0000955

Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant. (A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.
Figure Legend Snippet: Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant. (A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.

Techniques Used: Preserving, Infection, Concentration Assay

Related Articles

Incubation:

Article Title: Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy
Article Snippet: .. After 2 h incubation, samples were washed five times with PBS-T and reacted with the biotinylated anti-bovine TNF-α rabbit antibody13 for 1 h. After washing for five times with PBS-T, peroxidase-labeled Pierce™ NeutrAvidin™ Protein1 was added and incubated for 1 h. Finally, the mixture was washed five times with PBS-T and reacted with the TMB one component substrate for 10 min in the dark. ..

Article Title: Dural lymphatics regulate clearance of extracellular tau from the CNS
Article Snippet: .. Sections were incubated with LYVE1-e660 (ThermoFisher) at 1:200 overnight at 40 C. Sections were washed in PBS-T prior to mounting on slides in Prolong Gold antifade reagent with DAPI (ThermoFisher) mounting medium. .. Slides were imaged on Zeiss Axio Imager Z2 fluorescence microscope and Cytation 5 imaging reader (Biotek Imaging, Inc.).

Article Title: A Single-Stranded DNA Aptamer That Selectively Binds to Staphylococcus aureus Enterotoxin B
Article Snippet: .. Afterwards, the beads were again washed 3 times with 500 µl PBS-T. After the final wash was removed, 50 µl of 1X LDS sample buffer (Life Technologies) supplemented with 0.5 M NaCl was added on top of the coated beads, and the mixture was incubated at 50°C with agitation for 10 minutes. .. For a positive control, standards of BSA and SEB (100 ng, each) were diluted into 50 µl of 1X LDS loading buffer (Life Technologies) supplemented with 0.5 M NaCl.

Article Title: A Coproantigen Diagnostic Test for Strongyloides Infection
Article Snippet: .. The plates were again washed 4 times in PBS-T and 50 µl of NeutrAvidin-HRP (Thermo Scientific) diluted 1∶10,000 was added, and the plates incubated for a further 60 min at room temperature. ..

Article Title: A switch in transcription and cell fate governs the onset of an epigenetically-deregulated tumor in Drosophila
Article Snippet: .. After washing with 0.1% PBS-T for 15 min, DAPI (Invitrogen #62248, 1:500) was added and incubated for 15 min at RT. .. Imaginal discs were then dissected in PBS 1x and mounted in a slide with Vectashield mounting medium (Vector Laboratories).

Article Title: Dephosphorylation at a Conserved SP Motif Governs cAMP Sensitivity and Nuclear Localization of Class IIa Histone Deacetylases *
Article Snippet: .. After another set of six 8-min washes in PBS-T, membranes were incubated in PBS (two times for 5 min each) and then visualized on film after 5 min of incubation in Supersignal enhanced chemiluminescent solution (Pierce). .. The anti-HDAC4 antibody was affinity-purified from polyclonal rabbit antisera generated against an N-terminal fragment of HDAC4 ( ).

Article Title: Islands of retroelements are major components of Drosophila centromeres
Article Snippet: .. Slides were washed three times for 5 min in PBS-T and incubated with secondary antibodies (Life Technologies Alexa-488, 546, or 647 conjugated, 1:500) diluted in blocking solution and incubated at room temperature for 1 h or overnight at 4°C. ..

Blocking Assay:

Article Title: Islands of retroelements are major components of Drosophila centromeres
Article Snippet: .. Slides were washed three times for 5 min in PBS-T and incubated with secondary antibodies (Life Technologies Alexa-488, 546, or 647 conjugated, 1:500) diluted in blocking solution and incubated at room temperature for 1 h or overnight at 4°C. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher pbs pbs t pbs prolong gold antifade mountant
    Removing the top coverslip 20 μl is an excessive volume for mounting 22×22 mm coverslips, so the top coverslip should be easily removed by flicking the slide. If the top coverslip does not readily slide off the fiber coverslip, place the microscope slide in a container with enough volume of 2×SSC (after hybridization) or <t>PBS-T</t> (after an amplification layer) to cover the slide and gently shake the slide until the top coverslip floats off.
    Pbs Pbs T Pbs Prolong Gold Antifade Mountant, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs pbs t pbs prolong gold antifade mountant/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs pbs t pbs prolong gold antifade mountant - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    95
    Thermo Fisher pbs t
    Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant. (A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in <t>PBS-T</t> and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.
    Pbs T, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Thermo Fisher
    Average 95 stars, based on 3038 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Removing the top coverslip 20 μl is an excessive volume for mounting 22×22 mm coverslips, so the top coverslip should be easily removed by flicking the slide. If the top coverslip does not readily slide off the fiber coverslip, place the microscope slide in a container with enough volume of 2×SSC (after hybridization) or PBS-T (after an amplification layer) to cover the slide and gently shake the slide until the top coverslip floats off.

    Journal: Current protocols in microbiology

    Article Title: Human Papillomavirus Integration: Analysis by Molecular Combing and Fiber-FISH

    doi: 10.1002/cpmc.61

    Figure Lengend Snippet: Removing the top coverslip 20 μl is an excessive volume for mounting 22×22 mm coverslips, so the top coverslip should be easily removed by flicking the slide. If the top coverslip does not readily slide off the fiber coverslip, place the microscope slide in a container with enough volume of 2×SSC (after hybridization) or PBS-T (after an amplification layer) to cover the slide and gently shake the slide until the top coverslip floats off.

    Article Snippet: Shaker Coplin jars 0.2 M NaOH 70%, 90%, 100% EtOH 22×22 mm, #1.5 glass coverslips Hybridization buffer (see recipe) Human Cot-1 DNA (ThermoFisher, 15279011) Biotinylated HPV probe (Basic Protocol 2) Rubber cement (e.g., Elmer’s) 2× SSC 2× SSC/50% formamide (deionized, ThermoFisher, AM9342) 5% BSA in PBS (BSA/PBS) (IgG- and protease-free, Jackson Immunoresearch, 001-000-162) Biotinylated anti-streptavidin antibody (Vector Laboratories, BA-0500) Streptavidin, Alexa Fluor® 488 conjugate (ThermoFisher, ) Mouse anti-DNA antibody, single stranded, clone 16–19, IgG2a (EMD Millipore, MAB3034) Goat anti-mouse IgG2a Cross-Adsorbed secondary antibody, Alexa Fluor® 647 (Invitrogen, A-21241) 0.1% Triton X-100 in PBS (PBS-T) PBS ProLong® Gold Antifade Mountant (ThermoFisher, ) ThermoBrite® Slide Denaturation and Hybridization System and Slide Warmer (Leica) Place slides with baked fiber coverslips from Basic Protocol 3 in a coplin jar and denature DNA fibers for 20 minutes in 0.2 M NaOH.

    Techniques: Microscopy, Hybridization, Amplification

    Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant. (A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.

    Journal: PLoS Neglected Tropical Diseases

    Article Title: A Coproantigen Diagnostic Test for Strongyloides Infection

    doi: 10.1371/journal.pntd.0000955

    Figure Lengend Snippet: Formalin preservation allows specific discrimination of infected rat fecal supernatant from uninfected fecal supernatant. (A) Effect of assay specificity after various treatments. Pooled uninfected fecal supernatant collected from 6 rats (nRFS) and infected rat fecal supernatant collected from 2 rats infected with S. ratti (13 and 21 dpi) (iRFS) were extracted simultaneously in PBS-T and 4% formalin (4%F). Only extraction in formalin led to a positive discrimination between nRFS and iRFS. All points are mean ± SEM of OD values obtained from duplicate samples. (B) Detection of known amounts of E/S products diluted in PBS or uninfected rat fecal supernatant (nRFS) extracted in 4% formalin. The lowest concentration of E/S products detected was 80 ng/ml and 325 ng/ml when diluted in PBS and nRFS, respectively. All points are mean ± SEM of OD values obtained from 4 samples.

    Article Snippet: The plates were again washed 4 times in PBS-T and 50 µl of NeutrAvidin-HRP (Thermo Scientific) diluted 1∶10,000 was added, and the plates incubated for a further 60 min at room temperature.

    Techniques: Preserving, Infection, Concentration Assay