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Becton Dickinson pbs t
Human bocavirus (HBoV)-specific IgG avidities are shown as the means and standard deviation of avidity index (AI). Sera were collected from mice on study week 8 and diluted 1 : 200 in <t>PBS-T.</t> The AI was calculated as follows[AI = (OD 450 with urea)/(OD 450 without urea) × 100%],analysis of variance was used to compare the IgG avidity indexes between immunization via intradermal (i.d.) and intramuscular (i.m.) routes with and without alum adjuvant The Student's t -test was used to compare the IgG avidity index between HBoV1 and HBoV2 virus-like particles (VLPs). * P
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Article Title: Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice

Journal: Immunology

doi: 10.1111/imm.12202

Human bocavirus (HBoV)-specific IgG avidities are shown as the means and standard deviation of avidity index (AI). Sera were collected from mice on study week 8 and diluted 1 : 200 in PBS-T. The AI was calculated as follows[AI = (OD 450 with urea)/(OD 450 without urea) × 100%],analysis of variance was used to compare the IgG avidity indexes between immunization via intradermal (i.d.) and intramuscular (i.m.) routes with and without alum adjuvant The Student's t -test was used to compare the IgG avidity index between HBoV1 and HBoV2 virus-like particles (VLPs). * P
Figure Legend Snippet: Human bocavirus (HBoV)-specific IgG avidities are shown as the means and standard deviation of avidity index (AI). Sera were collected from mice on study week 8 and diluted 1 : 200 in PBS-T. The AI was calculated as follows[AI = (OD 450 with urea)/(OD 450 without urea) × 100%],analysis of variance was used to compare the IgG avidity indexes between immunization via intradermal (i.d.) and intramuscular (i.m.) routes with and without alum adjuvant The Student's t -test was used to compare the IgG avidity index between HBoV1 and HBoV2 virus-like particles (VLPs). * P

Techniques Used: Standard Deviation, Mouse Assay

Related Articles

Incubation:

Article Title: Hypoxia promotes stem cell phenotypes and poor prognosis through epigenetic regulation of DICER
Article Snippet: .. After washing sections in PBS, sections were permeabilized in PBS containing 0.5% Triton X-100 for 15 minutes, wash 3×5 mins in PBS-T and then incubated in a primary antibody cocktail of mouse anti-human CD44 (1:75, BD Pharmingen) and rat anti-mouse CD31 (1:300, BD Pharmingen) or anti-human H3K27me3 (1:500, C36B11; Cell Signalling) overnight at room temperature. .. Sections were subsequently washed 3×5 mins in PBS-T followed by incubation in a secondary antibody cocktail of goat anti-mouse Alexafluor 488 (1:200, Life Technologies) and goat anti-rat Alexafluor 555 (1:200, Life technologies) or goat anti-mouse Alexafluor 488 (1:200, Life Technologies) for 1 hour at room temperature.

Article Title: Testicular endothelial cells are a critical population in the germline stem cell niche
Article Snippet: .. Testis sections (10 or 30 μM) were blocked (5% normal goat serum and 0.1% bovine serum albumin in 0.1% phosphate-buffered saline with Tween-20 (PBS-T)) for 1 h and washed in 0.1% PBS-T. Primary antibodies, anti-CD31 rat-pAb (1:50; BD science, Cat: 550274), anti-PLZF rabbit polyclonal Ab (1:50; Santa Cruz, Cat: 22839), anti-DDX4 rabbit pAb (1:200; Abcam, Cat: 13840), anti-Sox9 rabbit pAb (1:200; Millipore, Cat: ABE579), anti-GDNF rabbit polyclonal Ab (1:50; Santa Cruz, Cat: SC328), were diluted in blocking buffer and incubated for 2 h at room temperature (RT), then incubated with either anti-mouse Alexa 594 (1:1000, Thermo Fischer, Cat: A110323) or anti-rabbit Alexa 488 (1:1000, Thermo Fischer, Cat: A32723) at RT for 30 min. .. Immunofluorescence images of testis regular sections were captured with AxioVision software (Zeiss) mounted on a Zeiss Imager M2 microscope or 30 μM thickness sections were captured Z -stack with Zeiss LSM 710 confocal, then all Z -stack images were reconstructed as a projection images by ImageJ (National Institutes of Health).

Article Title: Immunogenicity of recombinant human bocavirus-1,2 VP2 gene virus-like particles in mice
Article Snippet: .. After aspiration of the cell suspension, wells were washed first with deionized water and then with PBS-T. Then, 0·2 µg biotin-labelled anti-mouse IFN- γ antibody (BD Biosciences) was added and incubated for 2 hr at room temperature. .. After washing three times with PBS-T, horseradish peroxidase-labelled streptavidin was added at a dilution of 1 : 100, and incubated for 1 hr at room temperature.

Article Title: AMPA Receptors Commandeer an Ancient Cargo Exporter for Use as an Auxiliary Subunit for Signaling
Article Snippet: .. Then cells were incubated with the respective primary antibodies in 2% NGS in PBS-T for 1 hour at RT (rabbit anti-CNIH-2, 1∶250, ; mouse anti-GM130, 1∶100, BD Transduction Laboratories; chicken anti-MAP2, 1∶10.000, Abcam). .. Immunoreactivity was finally visualized by secondary anti-mouse, anti-rabbit and anti-chicken antibodies conjugated to cy-2, cy-3, or cy-5 (1∶250 in 10% NGS in PBS-T, Dianova).

Article Title: The Essential Role Played by B Cells in Supporting Protective Immunity Against Trichuris muris Infection Is by Controlling the Th1/Th2 Balance in the Mesenteric Lymph Nodes and Depends on Host Genetic Background
Article Snippet: .. Non-specific binding were blocked with 100 μl of 3% bovine serum albumin (BSA) (Melford Laboratories)/PBS at 37°C for 45 min. Plates were washed three times with PBS-T, and 50 μl of double diluted serum in PBS (1:20, 1:40, 1:80, 1:160, 1:320, 1:640, 1:1280, 1:2560) was added to plates and incubated for 60 min. Plates were washed three times with PBS-T and 50 μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a/c (1:1,000, BD Bioscience), or rat anti-mouse IgM (1:500, BD Bioscience) added to wells and incubated for 60 min. Plates were then washed with PBS-T three times before the plates were incubated with 75 μl of Streptavidin peroxidase (1:1,000, Sigma) for 60 min. Plates were washed three times with PBS-T and then the substrate, 100 μl of 0.03% hydrogen peroxidase-activated ABTS (10% 2,2'azino 3-thyl benzthiazoline in 0.045 M citrate buffer), was added. .. Plates were read at 450 nm on a VersaMax Microplate reader (Molecular Devices).

Article Title: Autophagy modulates SNCA/α-synuclein release, thereby generating a hostile microenvironment
Article Snippet: .. After air-drying, the membrane was blocked with 1% BSA in PBS-T at RT for 15 min and incubated with primary antibody against SNCA (1:1,000; BD Transduction Laboratories) or CD63 (1:200; SantaCruz, sc-15363) overnight at 4°C. .. After washing with PBS-Tween the membrane was incubated with HRP-conjugated goat anti-mouse secondary antibody (1:10,000, Jackson Immuno, 115–035–068) for 1 h at RT.

Binding Assay:

Article Title: The Essential Role Played by B Cells in Supporting Protective Immunity Against Trichuris muris Infection Is by Controlling the Th1/Th2 Balance in the Mesenteric Lymph Nodes and Depends on Host Genetic Background
Article Snippet: .. Non-specific binding were blocked with 100 μl of 3% bovine serum albumin (BSA) (Melford Laboratories)/PBS at 37°C for 45 min. Plates were washed three times with PBS-T, and 50 μl of double diluted serum in PBS (1:20, 1:40, 1:80, 1:160, 1:320, 1:640, 1:1280, 1:2560) was added to plates and incubated for 60 min. Plates were washed three times with PBS-T and 50 μl of either biotinylated rat anti-mouse IgG1 (1:500, BD Bioscience), rat anti-mouse IgG2a/c (1:1,000, BD Bioscience), or rat anti-mouse IgM (1:500, BD Bioscience) added to wells and incubated for 60 min. Plates were then washed with PBS-T three times before the plates were incubated with 75 μl of Streptavidin peroxidase (1:1,000, Sigma) for 60 min. Plates were washed three times with PBS-T and then the substrate, 100 μl of 0.03% hydrogen peroxidase-activated ABTS (10% 2,2'azino 3-thyl benzthiazoline in 0.045 M citrate buffer), was added. .. Plates were read at 450 nm on a VersaMax Microplate reader (Molecular Devices).

Next-Generation Sequencing:

Article Title: AMPA Receptors Commandeer an Ancient Cargo Exporter for Use as an Auxiliary Subunit for Signaling
Article Snippet: .. Then cells were incubated with the respective primary antibodies in 2% NGS in PBS-T for 1 hour at RT (rabbit anti-CNIH-2, 1∶250, ; mouse anti-GM130, 1∶100, BD Transduction Laboratories; chicken anti-MAP2, 1∶10.000, Abcam). .. Immunoreactivity was finally visualized by secondary anti-mouse, anti-rabbit and anti-chicken antibodies conjugated to cy-2, cy-3, or cy-5 (1∶250 in 10% NGS in PBS-T, Dianova).

Blocking Assay:

Article Title: Testicular endothelial cells are a critical population in the germline stem cell niche
Article Snippet: .. Testis sections (10 or 30 μM) were blocked (5% normal goat serum and 0.1% bovine serum albumin in 0.1% phosphate-buffered saline with Tween-20 (PBS-T)) for 1 h and washed in 0.1% PBS-T. Primary antibodies, anti-CD31 rat-pAb (1:50; BD science, Cat: 550274), anti-PLZF rabbit polyclonal Ab (1:50; Santa Cruz, Cat: 22839), anti-DDX4 rabbit pAb (1:200; Abcam, Cat: 13840), anti-Sox9 rabbit pAb (1:200; Millipore, Cat: ABE579), anti-GDNF rabbit polyclonal Ab (1:50; Santa Cruz, Cat: SC328), were diluted in blocking buffer and incubated for 2 h at room temperature (RT), then incubated with either anti-mouse Alexa 594 (1:1000, Thermo Fischer, Cat: A110323) or anti-rabbit Alexa 488 (1:1000, Thermo Fischer, Cat: A32723) at RT for 30 min. .. Immunofluorescence images of testis regular sections were captured with AxioVision software (Zeiss) mounted on a Zeiss Imager M2 microscope or 30 μM thickness sections were captured Z -stack with Zeiss LSM 710 confocal, then all Z -stack images were reconstructed as a projection images by ImageJ (National Institutes of Health).

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    Becton Dickinson pb t cells
    CXCR4 and <t>CCR5</t> are co-recruited into the IS. ( A ) Jurkat cells expressing GFP-tagged CCR5 or CXCR4 were incubated with SEE-loaded B cells for 10 min in the presence of AMD3100, TAK779, CXCL12 or CCL5. ( B ) <t>PB</t> T cells expressing GFP-tagged CCR5 or CXCR4
    Pb T Cells, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pb t cells/product/Becton Dickinson
    Average 88 stars, based on 1 article reviews
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    pb t cells - by Bioz Stars, 2020-07
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    Becton Dickinson pbs t
    CXCR4 and <t>CCR5</t> are co-recruited into the IS. ( A ) Jurkat cells expressing GFP-tagged CCR5 or CXCR4 were incubated with SEE-loaded B cells for 10 min in the presence of AMD3100, TAK779, CXCL12 or CCL5. ( B ) <t>PB</t> T cells expressing GFP-tagged CCR5 or CXCR4
    Pbs T, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t/product/Becton Dickinson
    Average 92 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    pbs t - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    CXCR4 and CCR5 are co-recruited into the IS. ( A ) Jurkat cells expressing GFP-tagged CCR5 or CXCR4 were incubated with SEE-loaded B cells for 10 min in the presence of AMD3100, TAK779, CXCL12 or CCL5. ( B ) PB T cells expressing GFP-tagged CCR5 or CXCR4

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CXCR4-CCR5: A couple modulating T cell functions

    doi: 10.1073/pnas.0804286105

    Figure Lengend Snippet: CXCR4 and CCR5 are co-recruited into the IS. ( A ) Jurkat cells expressing GFP-tagged CCR5 or CXCR4 were incubated with SEE-loaded B cells for 10 min in the presence of AMD3100, TAK779, CXCL12 or CCL5. ( B ) PB T cells expressing GFP-tagged CCR5 or CXCR4

    Article Snippet: The expression of CCR5 and CXCR4 on Jurkat, A7 and PB T cells was assessed by flow cytometry analysis (FACSCalibur or FACS Canto; Becton Dickinson) by using the commercial anti-human CCR5 mAb (R & D, clone: 45531; or R & D, clone: CTC5) and the commercial anti-CXCR4 mAb (BD PharMingen, clone: 12G5; or R & D, clone: 44717).

    Techniques: Expressing, Incubation

    CXCL12-induced costimulation requires CCR5 expression. IFN-γ production in resting ( A ), activated ( B ), CCR5-transfected resting ( C ), or CCR5Δ32 activated ( D ) human PB T cells stimulated with beads coated with anti-CD3 mAb in the presence

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: CXCR4-CCR5: A couple modulating T cell functions

    doi: 10.1073/pnas.0804286105

    Figure Lengend Snippet: CXCL12-induced costimulation requires CCR5 expression. IFN-γ production in resting ( A ), activated ( B ), CCR5-transfected resting ( C ), or CCR5Δ32 activated ( D ) human PB T cells stimulated with beads coated with anti-CD3 mAb in the presence

    Article Snippet: The expression of CCR5 and CXCR4 on Jurkat, A7 and PB T cells was assessed by flow cytometry analysis (FACSCalibur or FACS Canto; Becton Dickinson) by using the commercial anti-human CCR5 mAb (R & D, clone: 45531; or R & D, clone: CTC5) and the commercial anti-CXCR4 mAb (BD PharMingen, clone: 12G5; or R & D, clone: 44717).

    Techniques: Expressing, Transfection