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TaKaRa pbs t vector
Pbs T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs t vector/product/TaKaRa
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
pbs t vector - by Bioz Stars, 2020-07
88/100 stars

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Clone Assay:

Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis)
Article Snippet: .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). .. Forward and reverse M13 primers were used to screen for positive clones via PCR.

Article Title: Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus) Families
Article Snippet: .. According to the standard protocol, the purified products were ligated into a PBS-T vector with a TA cloning kit (Takara ) and then cloned into TOP 10 Escherichia coli competent cells (TransGen Biotech ). .. Positive clones were screened in PCR reactions with the cloning primers T7 and M13R.

Agarose Gel Electrophoresis:

Article Title: Cloning and characterization of two Argonaute genes in wheat (Triticum aestivum L.)
Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5′ -Full RACE Kit and 3′ -Full RACE Core Set Ver.

TA Cloning:

Article Title: Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus) Families
Article Snippet: .. According to the standard protocol, the purified products were ligated into a PBS-T vector with a TA cloning kit (Takara ) and then cloned into TOP 10 Escherichia coli competent cells (TransGen Biotech ). .. Positive clones were screened in PCR reactions with the cloning primers T7 and M13R.

Purification:

Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis)
Article Snippet: .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). .. Forward and reverse M13 primers were used to screen for positive clones via PCR.

Article Title: Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus) Families
Article Snippet: .. According to the standard protocol, the purified products were ligated into a PBS-T vector with a TA cloning kit (Takara ) and then cloned into TOP 10 Escherichia coli competent cells (TransGen Biotech ). .. Positive clones were screened in PCR reactions with the cloning primers T7 and M13R.

Sequencing:

Article Title: Cloning and characterization of two Argonaute genes in wheat (Triticum aestivum L.)
Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5′ -Full RACE Kit and 3′ -Full RACE Core Set Ver.

Transformation Assay:

Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis)
Article Snippet: .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). .. Forward and reverse M13 primers were used to screen for positive clones via PCR.

Plasmid Preparation:

Article Title: MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis)
Article Snippet: .. The purified fragments were cloned into a PBS-T vector (Takara) according to the standard PBS-T vector protocol (Takara) and then transformed into TOP 10 Escherichia coli competent cells (TransGen Biotech). .. Forward and reverse M13 primers were used to screen for positive clones via PCR.

Article Title: Cloning and characterization of two Argonaute genes in wheat (Triticum aestivum L.)
Article Snippet: .. After the products were separated by 1% agarose gel electrophoresis, their bands were recovered and sub-cloned into the pBS-T vector (TakaRa, China) for sequencing. .. The RACE technique was performed with a TaKaRa kit (5′ -Full RACE Kit and 3′ -Full RACE Core Set Ver.

Article Title: Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder (Paralichthys olivaceus) Families
Article Snippet: .. According to the standard protocol, the purified products were ligated into a PBS-T vector with a TA cloning kit (Takara ) and then cloned into TOP 10 Escherichia coli competent cells (TransGen Biotech ). .. Positive clones were screened in PCR reactions with the cloning primers T7 and M13R.

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  • 85
    TaKaRa pb t paf vector
    Plasmid and doxycycline dose–response curves. ( A – C ) The foci yield and protein expression levels obtained varying ( A ) PBase, ( B ) PB-RB, and ( C ) <t>PB-T-PAF</t> construct 4 while keeping the other two plasmids constant. Error bars represent SD
    Pb T Paf Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pb t paf vector/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pb t paf vector - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    88
    TaKaRa pbs t vector
    Plasmid and doxycycline dose–response curves. ( A – C ) The foci yield and protein expression levels obtained varying ( A ) PBase, ( B ) PB-RB, and ( C ) <t>PB-T-PAF</t> construct 4 while keeping the other two plasmids constant. Error bars represent SD
    Pbs T Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs t vector/product/TaKaRa
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbs t vector - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Plasmid and doxycycline dose–response curves. ( A – C ) The foci yield and protein expression levels obtained varying ( A ) PBase, ( B ) PB-RB, and ( C ) PB-T-PAF construct 4 while keeping the other two plasmids constant. Error bars represent SD

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

    doi: 10.1073/pnas.1218620110

    Figure Lengend Snippet: Plasmid and doxycycline dose–response curves. ( A – C ) The foci yield and protein expression levels obtained varying ( A ) PBase, ( B ) PB-RB, and ( C ) PB-T-PAF construct 4 while keeping the other two plasmids constant. Error bars represent SD

    Article Snippet: To allow a comparison between the PB system and that of the classical integration method, the genes encoding each protein were subcloned into the PB-T-PAF vector ( ) and the pIRES-puro3 vector (Clontech; ).

    Techniques: Plasmid Preparation, Expressing, Construct

    The inducible PB system is efficient and consistent. ( A ) The PB system is composed of a plasmid encoding the target protein (PB-T-PAF or PB-T-RfA) and a plasmid encoding the rtTA inducer (PB-RB or RN). The PB-T-PAF plasmid expresses an N-terminal Protein

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Simple piggyBac transposon-based mammalian cell expression system for inducible protein production

    doi: 10.1073/pnas.1218620110

    Figure Lengend Snippet: The inducible PB system is efficient and consistent. ( A ) The PB system is composed of a plasmid encoding the target protein (PB-T-PAF or PB-T-RfA) and a plasmid encoding the rtTA inducer (PB-RB or RN). The PB-T-PAF plasmid expresses an N-terminal Protein

    Article Snippet: To allow a comparison between the PB system and that of the classical integration method, the genes encoding each protein were subcloned into the PB-T-PAF vector ( ) and the pIRES-puro3 vector (Clontech; ).

    Techniques: Plasmid Preparation