Structured Review

Bio-Rad pbs solution
a SDS-PAGE of blank LN homogenate, free dye, <t>bevacizumab</t> in <t>PBS</t> (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node
Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs solution/product/Bio-Rad
Average 99 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pbs solution - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "Pharmacokinetics, Lymph Node Uptake, and Mechanistic PK Model of Near-Infrared Dye-Labeled Bevacizumab After IV and SC Administration in Mice"

Article Title: Pharmacokinetics, Lymph Node Uptake, and Mechanistic PK Model of Near-Infrared Dye-Labeled Bevacizumab After IV and SC Administration in Mice

Journal: The AAPS Journal

doi: 10.1208/s12248-012-9342-9

a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node
Figure Legend Snippet: a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

Techniques Used: SDS Page

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Incubation:

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Purification:

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  • 92
    Bio-Rad pbs solution
    a SDS-PAGE of blank LN homogenate, free dye, <t>bevacizumab</t> in <t>PBS</t> (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node
    Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Bio-Rad
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Bio-Rad sterile pbs solution
    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of <t>22L</t> prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of <t>PBS,</t> were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.
    Sterile Pbs Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile pbs solution/product/Bio-Rad
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    sterile pbs solution - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Bio-Rad β lactamase pbs buffer solution
    UV–vis absorption of nitrocefin solution with PCo-PPB copolymers (PCo-PPB-1 as an example) after adding <t>β</t> -lactamase for 1 h. 50 μ L of nitrocefin (DMSO solution 1.0 mg/mL) and PCo-PPB copolymers with different amounts (100, 200, and 400 μ g) were added into 1 mL of H 2 O and stirred for 12 h. Then, 1 μ L of β -lactamase <t>PBS</t> buffer solution (0.1 mg/mL, determined by Biorad Protein Assay) was added to the above solution. A characteristic absorption peak appeared near 480 nm due to hydrolysis of the β -lactam ring in nitrocefin.
    β Lactamase Pbs Buffer Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β lactamase pbs buffer solution/product/Bio-Rad
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    β lactamase pbs buffer solution - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Journal: The AAPS Journal

    Article Title: Pharmacokinetics, Lymph Node Uptake, and Mechanistic PK Model of Near-Infrared Dye-Labeled Bevacizumab After IV and SC Administration in Mice

    doi: 10.1208/s12248-012-9342-9

    Figure Lengend Snippet: a SDS-PAGE of blank LN homogenate, free dye, bevacizumab in PBS (2 μg), bevacizumab–800CW conjugate (2 μg) in PBS and bevacizumab–800CW conjugate (2 μg) spiked in blank mouse lymph node

    Article Snippet: Briefly, 8 μL of 500 μg/mL of bevacizumab IRDye 800CW conjugate or 500 μg/mL of bevacizumab in PBS solution was mixed with 12 μL of sample buffer (Bio-Rad, 2% SDS w/v , 25% glycerol v/v , 0.001% bromophenol blue w/v , 62.5 mM Tris–HCl, pH 6.8) with 5% β-mercaptoethanol, and 10 μL of each mixture was loaded and separated on a 10% polyacrylamide gel.

    Techniques: SDS Page

    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Journal: Scientific Reports

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

    doi: 10.1038/s41598-018-25966-9

    Figure Lengend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Article Snippet: Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France).

    Techniques: Mouse Assay, Positron Emission Tomography

    Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Journal: Scientific Reports

    Article Title: Low doses of bioherbicide favour prion aggregation and propagation in vivo

    doi: 10.1038/s41598-018-25966-9

    Figure Lengend Snippet: Mice treated with 5 mg/kg of A6 compound exhibit a decreased lifespan and an increased PrP Sc burden. ( a ) Table summarizing the different groups of animals: prion inoculations, treatment performed, number of sick animals out of total number of animals (*means that in this group, one mouse was sacrificed while asymptomatic) and median survival time ± IQR (interquartile range). (b) Kaplan-Meier survival curves of mice intra-cerebrally (i.c.) inoculated with 5 μL of 22L prions, and treated with 50 μL of DMSO (n = 9, blue) or with 5 mg/kg of A6 (n = 9, red) by intraperitoneal (i.p.) route, (+22L + DM versus +22L + A6–5: non–parametric Mantel-Cox log-rank test, ** p- value = 0,0067). Healthy control mice, inoculated with 5 μL of PBS, were treated with 5 mg/kg of A6 (n = 5, black dashed, −22L + A6–5) or non-treated (n = 5, brown, −22L + DM). (c) Left panel: PET-blots analysis of coronal sections of control healthy mice non-inoculated with prions and treated with 5 mg/kg of A6 (−22L + A6–5) (1) ; mice inoculated with 22L prions and either treated with DMSO (+22L + DM) (2) or treated with A6 (+22L + A6–5) (3) . Mice were all sacrificed at the same time (149, 149 and 147 dpi respectively) either in an asymptomatic or sick stage. Right panel: PET-blots analysis of coronal sections of sick mice: +22L + DM (4) and +22L + A6–5 (5) sacrificed at the same time (162 and 163 dpi, respectively). Sections were labelled with SAF84 antibody to detect PrP Sc and revealed with Vectastain ABC-AmP kit.

    Article Snippet: Aggregation assay Brains from a terminally sick mouse (infected with 22L prions) or from a non-infected mouse were homogenized in 10% (w/v) sterile PBS solution using microbead-containing tubes and a Ribolysor apparatus (Biorad, Marnes la Coquette, France).

    Techniques: Mouse Assay, Positron Emission Tomography

    UV–vis absorption of nitrocefin solution with PCo-PPB copolymers (PCo-PPB-1 as an example) after adding β -lactamase for 1 h. 50 μ L of nitrocefin (DMSO solution 1.0 mg/mL) and PCo-PPB copolymers with different amounts (100, 200, and 400 μ g) were added into 1 mL of H 2 O and stirred for 12 h. Then, 1 μ L of β -lactamase PBS buffer solution (0.1 mg/mL, determined by Biorad Protein Assay) was added to the above solution. A characteristic absorption peak appeared near 480 nm due to hydrolysis of the β -lactam ring in nitrocefin.

    Journal: ACS infectious diseases

    Article Title: Trio Act of Boronolectin with Antibiotic-Metal Complexed Macromolecules toward Broad-Spectrum Antimicrobial Efficacy

    doi: 10.1021/acsinfecdis.7b00132

    Figure Lengend Snippet: UV–vis absorption of nitrocefin solution with PCo-PPB copolymers (PCo-PPB-1 as an example) after adding β -lactamase for 1 h. 50 μ L of nitrocefin (DMSO solution 1.0 mg/mL) and PCo-PPB copolymers with different amounts (100, 200, and 400 μ g) were added into 1 mL of H 2 O and stirred for 12 h. Then, 1 μ L of β -lactamase PBS buffer solution (0.1 mg/mL, determined by Biorad Protein Assay) was added to the above solution. A characteristic absorption peak appeared near 480 nm due to hydrolysis of the β -lactam ring in nitrocefin.

    Article Snippet: 50 μ L of nitrocefin (DMSO solution 1.0 mg/mL) and PCo-PPB copolymers with different amounts (100, 200, and 400 μ g) were added into 1 mL of H2 O and stirred for 12 h. Then, 1 μ L of β -lactamase PBS buffer solution (0.1 mg/mL, determined by Biorad Protein Assay) was added to the above solution.

    Techniques: