pbs casein solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pbs casein solution
    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of <t>PBS.</t> At 10 and 25 days, serum samples were taken to determine the titers of <t>IgG</t> subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Pbs Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs casein solution/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs casein solution - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer"

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    Journal: Journal of Immunology Research

    doi: 10.1155/2019/7076942

    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Figure Legend Snippet: Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Techniques Used: Mouse Assay, Indirect ELISA

    Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P
    Figure Legend Snippet: Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Techniques Used: Mouse Assay, Indirect ELISA

    2) Product Images from "Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer"

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    Journal: Journal of Immunology Research

    doi: 10.1155/2019/7076942

    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Figure Legend Snippet: Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Techniques Used: Mouse Assay, Indirect ELISA

    Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P
    Figure Legend Snippet: Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Techniques Used: Mouse Assay, Indirect ELISA

    Related Articles

    ALP Assay:

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
    Article Snippet: .. Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C. .. The wells were washed and subsequently developed via a 20-minute incubation at 37°C with 100 μ l of a 1 mg/ml solution of pNpp (Merck) in ALP buffer.

    Incubation:

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
    Article Snippet: .. Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C. .. The wells were washed and subsequently developed via a 20-minute incubation at 37°C with 100 μ l of a 1 mg/ml solution of pNpp (Merck) in ALP buffer.

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
    Article Snippet: .. Subsequently, 100 μ l of a 1% PBS-casein solution containing goat serum anti-mouse IgG subclasses (i.e., IgG1, IgG2a, IgG2b, and IgG3) conjugated to HRP (1 : 10,000 dilution) from Thermo Scientific was added and incubated for 30 minutes at 37°C. .. The plates were washed in the same manner as described above, and 100 μ l of 1-Step™ Ultra TMB-ELISA solution (Thermo Scientific) was added.

    Labeling:

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer
    Article Snippet: .. Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C. .. The wells were washed and subsequently developed via a 20-minute incubation at 37°C with 100 μ l of a 1 mg/ml solution of pNpp (Merck) in ALP buffer.

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    Thermo Fisher pbs solution
    Pbs Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs solution/product/Thermo Fisher
    Average 99 stars, based on 369 article reviews
    Price from $9.99 to $1999.99
    pbs solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    93
    Thermo Fisher pbs casein solution
    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of <t>PBS.</t> At 10 and 25 days, serum samples were taken to determine the titers of <t>IgG</t> subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P
    Pbs Casein Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbs casein solution/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbs casein solution - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Humoral and cellular immune responses of the mice from the prophylactic bioassay utilizing the oral cancer orthotopic model consisting of C3H/He mice challenged with the AT-84 E7 Luc cell line. Groups of five female C3H/He mice were immunized with one of the following treatments: 100 μ g of KLH or FLH, 50 μ g of KLH or FLH plus 10 μ g of QS-21, and 10 μ g of QS-21 or 100 μ l of PBS. At 10 and 25 days, serum samples were taken to determine the titers of IgG subclasses by indirect ELISA. DTH determination was performed on day 15 of the bioassay. (a) Total anti-hemocyanin IgG titers in mouse sera. Data are shown as means ± SEM. Anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with QS-21 at day 10 and at day 21. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA

    Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Journal: Journal of Immunology Research

    Article Title: Immunotherapeutic Potential of Mollusk Hemocyanins in Combination with Human Vaccine Adjuvants in Murine Models of Oral Cancer

    doi: 10.1155/2019/7076942

    Figure Lengend Snippet: Specific humoral and cellular immune responses in C57BL/6 mice immunized with hemocyanins in combination with adjuvants. Groups of three female C57BL/6 mice were immunized subcutaneously on days 1 and 16 with one of the following treatments: 50 μ g of KLH, CCH, or FLH alone or in combination with 100 μ g of alum or 10 μ g of QS-21 in a 1 : 1 (vol/vol) ratio with AddaVax, all in a total volume of 100 μ l of PBS (vehicle). A serum sample was taken from each animal on day 37 after the second immunization for analysis by indirect ELISA. (a) Total anti-hemocyanin IgG titers in mouse sera. Values are presented as means ± SEM. The anti-hemocyanin IgG titers for each hemocyanin alone were compared by one-way ANOVA with the same hemocyanin in combination with an adjuvant. ∗∗∗∗ P

    Article Snippet: Subsequently, 100 μ l of a 1% PBS-casein solution containing goat anti-mouse IgG (H + L) serum labeled with alkaline phosphatase (ALP; Thermo Scientific) was added at a 1 : 2500 dilution and incubated for 30 minutes at 37°C.

    Techniques: Mouse Assay, Indirect ELISA