pbr322 dna bsuri haeiii marker  (Thermo Fisher)


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    Name:
    pBR322 DNA BsuRI HaeIII Marker
    Description:
    Thermo Scientific pBR322 DNA BsuRI HaeIII Marker is recommended for sizing and approximate quantification of small linear double stranded DNA fragments in agarose and non denaturing polyacrylamide gels pBR322 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme then purified and dissolved in storage buffer The DNA fragments contain blunt or sticky ends depending on the restriction enzyme used for the marker s preparationThe marker can be labeled radioactively with T4 Polynucleotide Kinase see Resources for protocol Highlights• Sizing and approximate quantification of small DNA fragments• Sharp bands• Supplied with loading dye for sample DNANote• Bands at 587 540 and 458 bp form an anomalous pattern on polyacrylamide gels • The shortest fragments oblique are not visible in standard electrophoresis • Fragment lengths are predicted by computer analysis of the respective DNA sequences
    Catalog Number:
    sm0271
    Price:
    None
    Category:
    Standards Ladders Controls
    Applications:
    Agarose Gel Electrophoresis|DNA & RNA Purification & Analysis|Nucleic Acid Gel Electrophoresis & Blotting
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    Structured Review

    Thermo Fisher pbr322 dna bsuri haeiii marker
    Thermo Scientific pBR322 DNA BsuRI HaeIII Marker is recommended for sizing and approximate quantification of small linear double stranded DNA fragments in agarose and non denaturing polyacrylamide gels pBR322 DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme then purified and dissolved in storage buffer The DNA fragments contain blunt or sticky ends depending on the restriction enzyme used for the marker s preparationThe marker can be labeled radioactively with T4 Polynucleotide Kinase see Resources for protocol Highlights• Sizing and approximate quantification of small DNA fragments• Sharp bands• Supplied with loading dye for sample DNANote• Bands at 587 540 and 458 bp form an anomalous pattern on polyacrylamide gels • The shortest fragments oblique are not visible in standard electrophoresis • Fragment lengths are predicted by computer analysis of the respective DNA sequences
    https://www.bioz.com/result/pbr322 dna bsuri haeiii marker/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbr322 dna bsuri haeiii marker - by Bioz Stars, 2021-03
    95/100 stars

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    Related Articles

    Marker:

    Article Title: The DNA methylation level against the background of the genome size and t-heterochromatin content in some species of the genus Secale L
    Article Snippet: .. Simultaneously with the samples the pBR322 DNA/Bsu RI (Hae III) marker (Fermentas) was prepared and loaded onto gel. .. After electrophoresis bands were detected by silver staining technique using Silver Sequence™ DNA Sequencing System (Promega, Madison, USA), according to manufacturer’s protocol.

    Article Title: Transcriptome Analysis of Androgenic Gland for Discovery of Novel Genes from the Oriental River Prawn, Macrobrachium nipponense, Using Illumina Hiseq 2000
    Article Snippet: The PCR reaction conditions were as follows: the DNA was first denatured at 94°C for 3 min; followed by 30 cycles of denaturation at 94°C for 30 s, annealing at 50–58°C for 30 s, and elongation at 72°C for 40 s; with a final elongation at 72°C for 10 min. PCR products were size-fractionated on 6% polyacrylamide gels by silver staining. .. The pBR322 DNA/BsuRI (Hae III) marker (Fermentas, Shenzhen, China) was used to determine the allele sizes. .. POPGEN32 software was used to analyze allele frequencies, Ho , and He for each locus and statistical significance of HWE .

    Nucleic Acid Electrophoresis:

    Article Title: Intercalative DNA binding governs fluorescence enhancement of SYBR Gold
    Article Snippet: We used the channel with absorption and emission wavelengths of 494 nm and 518 nm, respectively, which are the closest match to those of SYBR Gold (495 nm and 537 nm, respectively) and read out the fluorescence intensities at 24 °C from the top. .. For the gel electrophoresis we added Gel Loading Dye Purple (6x) (NEB) to pBR322 DNA. .. We used 1%-agarose (Carl Roth) gels and TAE buffer (40 mM Tris, 20 mM acetic acid, and 1 mM EDTA, pH 8.6).

    Hybridization:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity
    Article Snippet: .. Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses. .. We also performed a genome-wide linkage analysis on the Brazilian family, which was genotyped using the same Affymetrix platform.

    BAC Assay:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity
    Article Snippet: .. Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses. .. We also performed a genome-wide linkage analysis on the Brazilian family, which was genotyped using the same Affymetrix platform.

    other:

    Article Title: Promoter scanning during transcription initiation in Saccharomyces cerevisiae: Pol II in the “shooting gallery”
    Article Snippet: We used Samtools [ ] to extract the alignments to build genome coverage for visualization and start and end position of sequenced DNA fragments.

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  • 95
    Thermo Fisher iii 8 dna
    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual <t>III-8.</t> Solid-shaded large
    Iii 8 Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iii 8 dna/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    iii 8 dna - by Bioz Stars, 2021-03
    95/100 stars
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    Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large

    Journal:

    Article Title: Linkage to chromosome 2q36.1 in autosomal dominant Dandy-Walker malformation with occipital cephalocele and evidence for genetic heterogeneity

    doi: 10.1007/s00439-008-0467-y

    Figure Lengend Snippet: Pedigree of the Vietnamese-American ADDWOC family. Generation number is listed along the left margin, and individuals are numbered underneath each symbol . Thus, the proband (indicated by an asterisk ) corresponds to individual III-8. Solid-shaded large

    Article Snippet: Comparative genomic hybridization of I-8 and III-8 DNA on a 1 Mb resolution BAC array ( ) failed to identify any significant gains or losses.

    Techniques: