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Sysmex Corporation pbmcs
Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as <t>pDCs,</t> 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells <t>(PBMCs)</t> treated with CpG alone.
Pbmcs, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Effect of Interleukin-29 on Interferon-α Secretion by Peripheral Blood Mononuclear Cells"

Article Title: Effect of Interleukin-29 on Interferon-α Secretion by Peripheral Blood Mononuclear Cells

Journal: Cell Journal (Yakhteh)

doi:

Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as pDCs, 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells (PBMCs) treated with CpG alone.
Figure Legend Snippet: Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as pDCs, 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells (PBMCs) treated with CpG alone.

Techniques Used: Flow Cytometry, Cytometry, Produced

Related Articles

Isolation:

Article Title: Hydroxychloroquine inhibits trained immunity - implications for COVID-19
Article Snippet: .. Cell counts of whole blood and isolated PBMCs were also analyzed using a sysmex XN-450 automated hematology analyzer (Sysmex). .. Training and inhibition experimentsHuman PBMCs were trained as described before.

Article Title: Long noncoding RNA profiling revealed differentially expressed lncRNAs associated with disease activity in PBMCs from patients with rheumatoid arthritis
Article Snippet: .. PBMCs were isolated using Ficoll density gradient centrifugation and were counted with a blood cell analyzer (Sysmex, Kobe, Japan). .. Total RNA was subsequently extracted from the PBMCs using TRIzol (Invitrogen, Carlsbad, CA, USA).

Article Title: Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes
Article Snippet: .. Total white blood cells in PBMCs were counted by a Sysmex XE-2100 automated hematology analyzer immediately after isolation (pre-freeze) and after cryopreservation and recovery (post-thaw). (A) The yield of PBMCs per mL of input blood pre-freeze. (B) The yield of PBMCs per mL of input blood post-thaw. (C) Percent of cell recovery post-thaw. ..

Gradient Centrifugation:

Article Title: Long noncoding RNA profiling revealed differentially expressed lncRNAs associated with disease activity in PBMCs from patients with rheumatoid arthritis
Article Snippet: .. PBMCs were isolated using Ficoll density gradient centrifugation and were counted with a blood cell analyzer (Sysmex, Kobe, Japan). .. Total RNA was subsequently extracted from the PBMCs using TRIzol (Invitrogen, Carlsbad, CA, USA).

Transferring:

Article Title: The risk factor of false‐negative and false‐positive for T‐ SPOT. TB in active tuberculosis. The risk factor of false‐negative and false‐positive for T‐SPOT.TB in active tuberculosis
Article Snippet: .. Transferring 100 μL of the PBMCs into new tubes and calculated the number of cell by automatic blood cell counter (XS‐1000i; Sysmex, Shanghai, China). .. Transferring 100 μL of the PBMCs into new tubes and calculated the number of cell by automatic blood cell counter (XS‐1000i; Sysmex, Shanghai, China).

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    Sysmex Corporation pbmcs
    Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as <t>pDCs,</t> 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells <t>(PBMCs)</t> treated with CpG alone.
    Pbmcs, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Sysmex Corporation
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    88
    Sysmex Corporation feline pbmcs
    Kinetics of innate cytokine expression in feline <t>PBMCs</t> after <t>IRM</t> stimulation. Feline PBMCs of one cat were incubated for 6, 12, 24 and 48 h with (A) 144 μg/ml dSLIM™, (B) 20 μg/ml R-848 and (C) 20 μg/ml Poly IC. mRNA expression of the indicated genes was measured by real-time qPCR and normalized to expression of two feline housekeeping genes ( GUSB and YWHAZ ). Depicted expression factors at each time point represent the ratio of measured mRNA levels in the IRM-stimulated samples compared to samples treated with a negative control. Only genes indicating at least a 2-fold modulation in their expression at any time point are shown. Data represent means of duplicate reactions for each time point. Standard deviations are shown as an indication for reproducibility.
    Feline Pbmcs, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/feline pbmcs/product/Sysmex Corporation
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    feline pbmcs - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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    Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as pDCs, 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells (PBMCs) treated with CpG alone.

    Journal: Cell Journal (Yakhteh)

    Article Title: Effect of Interleukin-29 on Interferon-α Secretion by Peripheral Blood Mononuclear Cells

    doi:

    Figure Lengend Snippet: Production of IFN-α in plasmacytoid dendritic cells (pDC) in response to CpG or IL-29 as measured by intracellular flow cytometry. A. I (control). Among cells gated as pDCs, 2.96% of cells produced IFN-α. B. II (CpG alone). The cells that produced IFN-α increased to 4.52% compared to the control. C. III (IL-29 alone). Among cells gated as pDC, 1.20% produced IFN-α. D. IV (CpG and IL- 29). A total of 3.95% of cells produced IFN-α which was similar to peripheral blood mononuclear cells (PBMCs) treated with CpG alone.

    Article Snippet: Absolute pDC numbers were estimated by multiplying the percentage of pDCs by the number of PBMCs, which was calculated by multiplying the number of white blood cells (WBCs) by the percentage of lymphocytes plus monocytes in WBCs as measured in the Sysmex XE-2100 apparatus (Sysmex Corporation; Kobe, Japan).

    Techniques: Flow Cytometry, Cytometry, Produced

    Kinetics of innate cytokine expression in feline PBMCs after IRM stimulation. Feline PBMCs of one cat were incubated for 6, 12, 24 and 48 h with (A) 144 μg/ml dSLIM™, (B) 20 μg/ml R-848 and (C) 20 μg/ml Poly IC. mRNA expression of the indicated genes was measured by real-time qPCR and normalized to expression of two feline housekeeping genes ( GUSB and YWHAZ ). Depicted expression factors at each time point represent the ratio of measured mRNA levels in the IRM-stimulated samples compared to samples treated with a negative control. Only genes indicating at least a 2-fold modulation in their expression at any time point are shown. Data represent means of duplicate reactions for each time point. Standard deviations are shown as an indication for reproducibility.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: The innate antiviral immune system of the cat: Molecular tools for the measurement of its state of activation

    doi: 10.1016/j.vetimm.2011.06.005

    Figure Lengend Snippet: Kinetics of innate cytokine expression in feline PBMCs after IRM stimulation. Feline PBMCs of one cat were incubated for 6, 12, 24 and 48 h with (A) 144 μg/ml dSLIM™, (B) 20 μg/ml R-848 and (C) 20 μg/ml Poly IC. mRNA expression of the indicated genes was measured by real-time qPCR and normalized to expression of two feline housekeeping genes ( GUSB and YWHAZ ). Depicted expression factors at each time point represent the ratio of measured mRNA levels in the IRM-stimulated samples compared to samples treated with a negative control. Only genes indicating at least a 2-fold modulation in their expression at any time point are shown. Data represent means of duplicate reactions for each time point. Standard deviations are shown as an indication for reproducibility.

    Article Snippet: For experiments including IRM stimulation or FIV inoculation of feline PBMCs, the isolated cells were counted using the Sysmex XT 2000 iV (Sysmex, Norderstedt, Germany) as previously described ( ) and dispersed in 96-well plates at a density of 3 × 105 cells per well in 100 μl complete RPMI.

    Techniques: Expressing, Incubation, Real-time Polymerase Chain Reaction, Negative Control

    Innate immune responses to FIV infection of feline PBMCs in vitro. PBMCs of one cat were inoculated with 50 TCID50 of FIV. mRNA expression of the indicated genes was measured 3, 6, 12, 24 and 48 h after inoculation and normalized to expression of two feline housekeeping genes ( GUSB and YWHAZ ) at each time point. Depicted expression factors at each time point represent the ratio of measured mRNA levels in the IRM-stimulated samples compared to samples treated with a negative control. Only genes indicating at least a 2-fold modulation in their expression at any time point are shown. Data represent means of duplicate reactions for each time point. Standard deviations are shown as an indication for reproducibility. GzmB = Granzyme B.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: The innate antiviral immune system of the cat: Molecular tools for the measurement of its state of activation

    doi: 10.1016/j.vetimm.2011.06.005

    Figure Lengend Snippet: Innate immune responses to FIV infection of feline PBMCs in vitro. PBMCs of one cat were inoculated with 50 TCID50 of FIV. mRNA expression of the indicated genes was measured 3, 6, 12, 24 and 48 h after inoculation and normalized to expression of two feline housekeeping genes ( GUSB and YWHAZ ) at each time point. Depicted expression factors at each time point represent the ratio of measured mRNA levels in the IRM-stimulated samples compared to samples treated with a negative control. Only genes indicating at least a 2-fold modulation in their expression at any time point are shown. Data represent means of duplicate reactions for each time point. Standard deviations are shown as an indication for reproducibility. GzmB = Granzyme B.

    Article Snippet: For experiments including IRM stimulation or FIV inoculation of feline PBMCs, the isolated cells were counted using the Sysmex XT 2000 iV (Sysmex, Norderstedt, Germany) as previously described ( ) and dispersed in 96-well plates at a density of 3 × 105 cells per well in 100 μl complete RPMI.

    Techniques: Infection, In Vitro, Expressing, Negative Control