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Cytoskeleton Inc pbmcs
Analysis of expression of activation surface markers in <t>PBMCs</t> stimulated with A. hydrophila ATCC ® 7966 TM <t>OMVs.</t> PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
Pbmcs, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbmcs/product/Cytoskeleton Inc
Average 93 stars, based on 4 article reviews
Price from $9.99 to $1999.99
pbmcs - by Bioz Stars, 2020-09
93/100 stars

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1) Product Images from "The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells"

Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.02765

Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
Figure Legend Snippet: Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

Techniques Used: Expressing, Activation Assay, Cell Culture, Flow Cytometry, Cytometry

Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P
Figure Legend Snippet: Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

Techniques Used: Crocin Bleaching Assay

Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P
Figure Legend Snippet: Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

Techniques Used: Expressing, Inhibition, Cell Culture, Flow Cytometry, Cytometry

Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P
Figure Legend Snippet: Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

Techniques Used: Cell Culture, Flow Cytometry, Cytometry

Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.
Figure Legend Snippet: Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

Techniques Used: Staining, Confocal Microscopy

2) Product Images from "The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells"

Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2018.02765

Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
Figure Legend Snippet: Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

Techniques Used: Expressing, Activation Assay, Cell Culture, Flow Cytometry, Cytometry

Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P
Figure Legend Snippet: Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

Techniques Used: Crocin Bleaching Assay

Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P
Figure Legend Snippet: Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

Techniques Used: Expressing, Inhibition, Cell Culture, Flow Cytometry, Cytometry

Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P
Figure Legend Snippet: Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

Techniques Used: Cell Culture, Flow Cytometry, Cytometry

Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.
Figure Legend Snippet: Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

Techniques Used: Staining, Confocal Microscopy

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    Cytoskeleton Inc pbmcs
    Analysis of expression of activation surface markers in <t>PBMCs</t> stimulated with A. hydrophila ATCC ® 7966 TM <t>OMVs.</t> PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
    Pbmcs, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs/product/Cytoskeleton Inc
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pbmcs - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    92
    Cytoskeleton Inc pbmcs aeromonas hydrophila omvs
    Analysis of expression of activation surface markers in <t>PBMCs</t> stimulated with A. <t>hydrophila</t> ATCC ® 7966 TM <t>OMVs.</t> PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
    Pbmcs Aeromonas Hydrophila Omvs, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmcs aeromonas hydrophila omvs/product/Cytoskeleton Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    pbmcs aeromonas hydrophila omvs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Cytoskeleton Inc pbmc cultures
    Analysis of expression of activation surface markers in <t>PBMCs</t> stimulated with A. <t>hydrophila</t> ATCC ® 7966 TM <t>OMVs.</t> PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P
    Pbmc Cultures, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbmc cultures/product/Cytoskeleton Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pbmc cultures - by Bioz Stars, 2020-09
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    Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    Article Snippet: Effect of OMVs on PBMCs Cytoskeleton PBMCs were fixed onto cover slips by duplicated and permeabilized with Triton X-100 0.5% in PBS.

    Techniques: Expressing, Activation Assay, Cell Culture, Flow Cytometry, Cytometry

    Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

    Article Snippet: Effect of OMVs on PBMCs Cytoskeleton PBMCs were fixed onto cover slips by duplicated and permeabilized with Triton X-100 0.5% in PBS.

    Techniques: Crocin Bleaching Assay

    Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

    Article Snippet: Effect of OMVs on PBMCs Cytoskeleton PBMCs were fixed onto cover slips by duplicated and permeabilized with Triton X-100 0.5% in PBS.

    Techniques: Expressing, Inhibition, Cell Culture, Flow Cytometry, Cytometry

    Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

    Article Snippet: Effect of OMVs on PBMCs Cytoskeleton PBMCs were fixed onto cover slips by duplicated and permeabilized with Triton X-100 0.5% in PBS.

    Techniques: Cell Culture, Flow Cytometry, Cytometry

    Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

    Article Snippet: Effect of OMVs on PBMCs Cytoskeleton PBMCs were fixed onto cover slips by duplicated and permeabilized with Triton X-100 0.5% in PBS.

    Techniques: Staining, Confocal Microscopy

    Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Analysis of expression of activation surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Activation was measured by flow cytometry using mAbs against surface molecules (CD69, CD86). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. ∗ P

    Article Snippet: A. hydrophila ATCC® 7966TM OMVs Affects Morphological Shape and Cytoskeleton Structure of PBMCs Aeromonas hydrophila OMVs (10 μg/mL) induced activation of lymphocytes cells from PBMCs; cell activation induced changes in morphology and cytoskeleton organization.

    Techniques: Expressing, Activation Assay, Cell Culture, Flow Cytometry, Cytometry

    Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Cytokine quantification from PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. Inflammatory cytokines were determined by CBA cytometric assay at different time points. A. hydrophila OMVs induced high levels of inflammatory cytokines such as IL-8, IL-1β, IL-6, and TNFα. ∗ P

    Article Snippet: A. hydrophila ATCC® 7966TM OMVs Affects Morphological Shape and Cytoskeleton Structure of PBMCs Aeromonas hydrophila OMVs (10 μg/mL) induced activation of lymphocytes cells from PBMCs; cell activation induced changes in morphology and cytoskeleton organization.

    Techniques: Crocin Bleaching Assay

    Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Analysis of expression of inhibition surface markers in PBMCs stimulated with A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with OMVs from A. hydrophila . Inhibition was measured by flow cytometry using mAbs against surface molecules (PD-1, PD-L1). Monocyte (CD91+) and lymphocytes (CD3+, CD19+) were gated to analyze individually the expression of surface molecules. FKB were used as control for the expression of surface markers. ∗ P

    Article Snippet: A. hydrophila ATCC® 7966TM OMVs Affects Morphological Shape and Cytoskeleton Structure of PBMCs Aeromonas hydrophila OMVs (10 μg/mL) induced activation of lymphocytes cells from PBMCs; cell activation induced changes in morphology and cytoskeleton organization.

    Techniques: Expressing, Inhibition, Cell Culture, Flow Cytometry, Cytometry

    Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Apoptosis and DNA damage of PBMCs induced by A. hydrophila ATCC ® 7966 TM OMVs. PBMCs from healthy donor were co-cultured with different concentrations of A. hydrophila OMVs during 24 h. Apoptosis and DNA damaged was evaluated by flow cytometry with mAbs anti-PARP and anti-H2AX. CCCP was used as control for apoptosis induction. ∗ P

    Article Snippet: A. hydrophila ATCC® 7966TM OMVs Affects Morphological Shape and Cytoskeleton Structure of PBMCs Aeromonas hydrophila OMVs (10 μg/mL) induced activation of lymphocytes cells from PBMCs; cell activation induced changes in morphology and cytoskeleton organization.

    Techniques: Cell Culture, Flow Cytometry, Cytometry

    Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

    Journal: Frontiers in Microbiology

    Article Title: The Outer Membrane Vesicles of Aeromonas hydrophila ATCC® 7966TM: A Proteomic Analysis and Effect on Host Cells

    doi: 10.3389/fmicb.2018.02765

    Figure Lengend Snippet: Morphological alterations induced by A. hydrophila ATCC ® 7966 TM OMVs in PBMCs. (A) After 2 h post-stimulation with A. hydrophila OMVs, no morphology changes of the PBMCs were observed compared with PBMCs after 24 h post-stimulation with vesicles, after 24 h post-stimulation conglomerated cells were observed (arrowheads) (B) . Giemsa staining revealed differences in the affinity of the dye to the nucleus and cytoplasm, bigger cells, and vacuolization of the stimulated PBMCs with A. hydrophila OMVs (arrowheads) (C) . Confocal microscopy, showed defective tubulin polymerization in PBMCs stimulated with OMVs (D) . bars = 50 μm.

    Article Snippet: A. hydrophila ATCC® 7966TM OMVs Affects Morphological Shape and Cytoskeleton Structure of PBMCs Aeromonas hydrophila OMVs (10 μg/mL) induced activation of lymphocytes cells from PBMCs; cell activation induced changes in morphology and cytoskeleton organization.

    Techniques: Staining, Confocal Microscopy